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Status of vaccination for cervical cancer in India
S90
Abstracts
Poster Session, Sunday 29 January 2017
696 POSTER Medicinal ShenLingLan influences ovarian cancer cell migratory behaviour potentially through the GSK-3 pathway
698 POSTER Perception of cervical cancer and human papillomavirus in Korean female university students
S. Owen1 , F. Ruge1 , Y. Gao2 , Y. Yang3 , J. Hou3 , J. Chen3 , Y. Gao4 , H. Wang5 , C. Wei5 , Y. Wu6 , W.G. Jiang1 . 1 Cardiff School of MedicineCardiff University, Cardiff China Medical Research Collabroative, CardiffWales, United Kingdom; 2 Peking University Cancer Hospital, Department of Gynaecological Oncology, Beijing, China; 3 YuHuangDing Hospital, Departments of Gynaecology and Medical Oncology, YanTai, China; 4 Yiling Medical Research Institute, Yiling Medical Research Institute, Heibei, China; 5 State Key Laboratory of Collateral Disease Research and Innovation, Yiling Medical Research Institute, HeiBei, China; 6 Key Disciplines of State Administration of TCM for Collateral Disease, Yiling Medical Research Institute, HeiBei, China
H. Kim1 . 1 Seoul National University, College of Nursing, Seoul, South Korea
Background: Ovarian cancer, referred to as the ‘silent killer’, is often diagnosed during the later stages of the disease. Up to twenty percent of cases at time of diagnosis have evidence of metastasis. Glycogen synthase kinase-3 (GSK-3) is a protein which has been identified with a wide variety of oncogenic traits including cell cycle progression, migration and therapy resistance. ShenLingLan (SLDM) is herbal medicinal formula in which early indications show promise as an anti-cancer agent. This study therefore aimed to determine the expression levels of GSK-3 in an ovarian clinical cohort and analyse how the addition of SLDM in vitro affected ovarian cancer cell migration. Materials and Methods: Phosphorylation changes in GSK-3 were identified using a KinexusTM protein kinase array using protein lysates from immortalised ovarian cancer cells. Fresh ovarian tumours (n = 113) were collected immediately after surgery and processed for histological and molecular analyses. Histopathologcial and clinical information including staging, differentiation and outcome were also collected and analysed against GSK-3 transcript levels using quantitative PCR (qPCR). The effects of SLDM on 3 different immortalised ovarian cell lines (SKOV-3, A2780 and COV504) and their ability to attach and migrate were evaluated using Electrical Cell-substrate Impedance Sensing (ECIS) both in the presence of SLDM and a combination of SLDM and GSK-3 small inhibitor. Results: Significantly increased GSK-3b transcript levels were seen in ovarian tumours which were poorly differentiated (p < 005, vs well differentiated), in patients who had recurrence (p < 005, vs disease free) and in those patients who had died from ovarian cancer (p < 005, vs patients who were alive). Treating SKOV-3 ovarian cells with SLDM resulted in a reduction in pan-specific GSK-3 and several specific phosphorylation sites of GSK-3 (Y279). Treatment with SLDM reduced all three ovarian cell lines’ attachment and migration in a concentration dependent manner, which was further reduced in the presence of TWS119, particularly in the SKOV-3 and A2780 cells. Conclusions: In ovarian cancer both isomers of GSK-3 have been linked to disease, GSK-alpha in chemo-resistance and GSK3-beta with ovarian cancer cell proliferation. In the clinical cohort GSK-3b transcript levels were associated with poor differentiation, recurrence and clinical outcome. Furthermore, this study identified a potential mechanism by which SLDM may have anti-metastatic effects by influencing ovarian cancer cell attachment and migration. No conflict of interest. 697 Status of vaccination for cervical cancer in India
POSTER
R. Kaushik1 . 1 MSM hospital, Gynecology, Kota, India Background: India contributes to almost 1/3rd of deaths due to cervical cancer in the world. Keeping this in mind it becomes imperative that the country should be focused on prevention of the disease through vaccination. But are we really doing so? We have done this study to find answers for this question. Materials and Methods: A review of literature, media reports, government records and statistics was done to find out the current state of affairs. Result: We found that the current rate of vaccination for cervical cancer is very low in India. The lower rate of pap smear screening confounds this problem. The main issue for vaccination is still awareness followed by financial constraints. That the vaccine is manufactured out of India and is imported adds to the cost. The health burden of cervical cancer on Indian society is huge and if there is no government intervention to reduce the cost or manufacture the vaccine in India this situation will worsen. Conclusion: Cervical cancer is a major killer and more so in the Indian context. If the situation is to be improved we must create awareness and also reduce the cost of the vaccine for it to be used by the masses. No conflict of interest.
Background: Cervical cancer is the second leading cause of death in women globally. HPV has known the primary causing factor of cervix cancer. Human papillomavirus (HPV) is highly prevalent sexually transmitted disease (STD) among young age group. Despite increased awareness of HPV and HPV vaccination in Korea, but there are lacking of preventing cervical cancer for unmarried women in Korea. This study examined the level of HPV knowledge and severity of cervical cancer in Korean female university students. Methods: A survey design was utilized to collect cross sectional and retrospective data. A convenient sample of 303 female students attending university in Korea were recruited in this study. The inclusion criteria was that the students were unmarried. Level of HPV knowledge was assessed using 8 items. The severity perception of the cervical cancer was assessed using 5 items. And Socio-demographic data were assessed. Data analysis procedures included descriptive analysis using SPSS package program. Results: 45.5% students answered heard of HPV and 41.9% answered heard of HPV vaccine. Almost were not get HPV vaccinated. The level of HPV knowledge was low in general. Most known HPV item was “HPV vaccine prevent HPV” (corrected answer, 75.2%) and most unknown HPV were “HPV is treated by drug or surgery” (8.2%), “Genital warts cause cervix cancer” (10.2%). In response to the severity of cervix cancer, most students answered “disagree” (4 score) or “neutral” (3 score) on all 5 items using 5 likert scale. Conclusions: HPV awareness among female university students was low in Korea. However, the students showed the attitude toward cervical cancer as not so severe or neutral. It is needed to focus on their perception related to preventive health behavior for preventing cervix cancer among unmarried women. Acknowledgement: This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2015R1-A1-A3A0-4001267). No conflict of interest. 699 POSTER Value of body mass index, pathological complete response, and residual tumors after complete cytoreductive surgery plus hyperthermic intraperitoneal chemotherapy for patients with recurrent ovarian cancers. Experience from MISR Cancer Center G. Amira1 , A. Sherif2 , I. Sallam1 , M. Sherif2 , K. Diab1 , A. Saber3 . 1 MISR Cancer Center, Surgical Oncology, Giza, Egypt; 2 MISR International Hospital, General and GIT Surgery, Cairo, Egypt; 3 MISR Cancer Center, Medical Oncology, Giza, Egypt Background: Most patients with primary ovarian cancer develop a recurrence that is associated with a poor prognosis. Cytoreductive surgery (CRS) and hyperthermic intraperitoneal chemotherapy HIPEC have a promising results in patients with Recurrent Ovarian Cancer (ROC). Pathologic complete response (pCR) after HIPEC is an important prognosticator in the management of ROC. As rates of obesity is high among Egyptian Females, in this study it is clear that body mass index (BMI) affects the likelihood of achieving pCR and complete CRS. We describe an analysis of the relationship between BMI at diagnosis and pCR (absence of residual or recurrent disease) in ROC cases. With unfavorable levels of complete CRS, another important predictor of management outcomes in patients with ROC. Methods: 39 Study participants were ROC presented to our center from 7/2012 to 11/2015 of which 28 patients underwent the primary surgery at MISR Cancer Center and 11 are referred from other centers. As part of a prospective trial on residual tumor and whose postoperative pathological review definitively described pCR. Clinical characteristics potentially associated with pCR including BMI, age, race, residual tumor size/biomarkers, nodal status, morbidity issues(fistulas and Chest complications) and the presence of extra-pelvic metastasis were examined, as were the relationships between BMI, pCR, and residual tumors. We reported proportions, adjusted odds ratios (OR), and 95% confidence intervals (CI) significant at 2-tailed p < 005. Results: Of 39 patients, 24 (61%) had pCR; 27 (69%) were obese (BMI 30), and 15 (38%) had residual tumor. There was a difference between obese and non-obese (BMI <30) patients’ pCR rates(61% [24/39] vs. 38% [15/39], p = 0.69) or residual tumor(38% [15/39] vs. 61% [24/39],
Abstracts
Poster Session, Sunday 29 January 2017
696 POSTER Medicinal ShenLingLan influences ovarian cancer cell migratory behaviour potentially through the GSK-3 pathway
698 POSTER Perception of cervical cancer and human papillomavirus in Korean female university students
S. Owen1 , F. Ruge1 , Y. Gao2 , Y. Yang3 , J. Hou3 , J. Chen3 , Y. Gao4 , H. Wang5 , C. Wei5 , Y. Wu6 , W.G. Jiang1 . 1 Cardiff School of MedicineCardiff University, Cardiff China Medical Research Collabroative, CardiffWales, United Kingdom; 2 Peking University Cancer Hospital, Department of Gynaecological Oncology, Beijing, China; 3 YuHuangDing Hospital, Departments of Gynaecology and Medical Oncology, YanTai, China; 4 Yiling Medical Research Institute, Yiling Medical Research Institute, Heibei, China; 5 State Key Laboratory of Collateral Disease Research and Innovation, Yiling Medical Research Institute, HeiBei, China; 6 Key Disciplines of State Administration of TCM for Collateral Disease, Yiling Medical Research Institute, HeiBei, China
H. Kim1 . 1 Seoul National University, College of Nursing, Seoul, South Korea
Background: Ovarian cancer, referred to as the ‘silent killer’, is often diagnosed during the later stages of the disease. Up to twenty percent of cases at time of diagnosis have evidence of metastasis. Glycogen synthase kinase-3 (GSK-3) is a protein which has been identified with a wide variety of oncogenic traits including cell cycle progression, migration and therapy resistance. ShenLingLan (SLDM) is herbal medicinal formula in which early indications show promise as an anti-cancer agent. This study therefore aimed to determine the expression levels of GSK-3 in an ovarian clinical cohort and analyse how the addition of SLDM in vitro affected ovarian cancer cell migration. Materials and Methods: Phosphorylation changes in GSK-3 were identified using a KinexusTM protein kinase array using protein lysates from immortalised ovarian cancer cells. Fresh ovarian tumours (n = 113) were collected immediately after surgery and processed for histological and molecular analyses. Histopathologcial and clinical information including staging, differentiation and outcome were also collected and analysed against GSK-3 transcript levels using quantitative PCR (qPCR). The effects of SLDM on 3 different immortalised ovarian cell lines (SKOV-3, A2780 and COV504) and their ability to attach and migrate were evaluated using Electrical Cell-substrate Impedance Sensing (ECIS) both in the presence of SLDM and a combination of SLDM and GSK-3 small inhibitor. Results: Significantly increased GSK-3b transcript levels were seen in ovarian tumours which were poorly differentiated (p < 005, vs well differentiated), in patients who had recurrence (p < 005, vs disease free) and in those patients who had died from ovarian cancer (p < 005, vs patients who were alive). Treating SKOV-3 ovarian cells with SLDM resulted in a reduction in pan-specific GSK-3 and several specific phosphorylation sites of GSK-3 (Y279). Treatment with SLDM reduced all three ovarian cell lines’ attachment and migration in a concentration dependent manner, which was further reduced in the presence of TWS119, particularly in the SKOV-3 and A2780 cells. Conclusions: In ovarian cancer both isomers of GSK-3 have been linked to disease, GSK-alpha in chemo-resistance and GSK3-beta with ovarian cancer cell proliferation. In the clinical cohort GSK-3b transcript levels were associated with poor differentiation, recurrence and clinical outcome. Furthermore, this study identified a potential mechanism by which SLDM may have anti-metastatic effects by influencing ovarian cancer cell attachment and migration. No conflict of interest. 697 Status of vaccination for cervical cancer in India
POSTER
R. Kaushik1 . 1 MSM hospital, Gynecology, Kota, India Background: India contributes to almost 1/3rd of deaths due to cervical cancer in the world. Keeping this in mind it becomes imperative that the country should be focused on prevention of the disease through vaccination. But are we really doing so? We have done this study to find answers for this question. Materials and Methods: A review of literature, media reports, government records and statistics was done to find out the current state of affairs. Result: We found that the current rate of vaccination for cervical cancer is very low in India. The lower rate of pap smear screening confounds this problem. The main issue for vaccination is still awareness followed by financial constraints. That the vaccine is manufactured out of India and is imported adds to the cost. The health burden of cervical cancer on Indian society is huge and if there is no government intervention to reduce the cost or manufacture the vaccine in India this situation will worsen. Conclusion: Cervical cancer is a major killer and more so in the Indian context. If the situation is to be improved we must create awareness and also reduce the cost of the vaccine for it to be used by the masses. No conflict of interest.
Background: Cervical cancer is the second leading cause of death in women globally. HPV has known the primary causing factor of cervix cancer. Human papillomavirus (HPV) is highly prevalent sexually transmitted disease (STD) among young age group. Despite increased awareness of HPV and HPV vaccination in Korea, but there are lacking of preventing cervical cancer for unmarried women in Korea. This study examined the level of HPV knowledge and severity of cervical cancer in Korean female university students. Methods: A survey design was utilized to collect cross sectional and retrospective data. A convenient sample of 303 female students attending university in Korea were recruited in this study. The inclusion criteria was that the students were unmarried. Level of HPV knowledge was assessed using 8 items. The severity perception of the cervical cancer was assessed using 5 items. And Socio-demographic data were assessed. Data analysis procedures included descriptive analysis using SPSS package program. Results: 45.5% students answered heard of HPV and 41.9% answered heard of HPV vaccine. Almost were not get HPV vaccinated. The level of HPV knowledge was low in general. Most known HPV item was “HPV vaccine prevent HPV” (corrected answer, 75.2%) and most unknown HPV were “HPV is treated by drug or surgery” (8.2%), “Genital warts cause cervix cancer” (10.2%). In response to the severity of cervix cancer, most students answered “disagree” (4 score) or “neutral” (3 score) on all 5 items using 5 likert scale. Conclusions: HPV awareness among female university students was low in Korea. However, the students showed the attitude toward cervical cancer as not so severe or neutral. It is needed to focus on their perception related to preventive health behavior for preventing cervix cancer among unmarried women. Acknowledgement: This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2015R1-A1-A3A0-4001267). No conflict of interest. 699 POSTER Value of body mass index, pathological complete response, and residual tumors after complete cytoreductive surgery plus hyperthermic intraperitoneal chemotherapy for patients with recurrent ovarian cancers. Experience from MISR Cancer Center G. Amira1 , A. Sherif2 , I. Sallam1 , M. Sherif2 , K. Diab1 , A. Saber3 . 1 MISR Cancer Center, Surgical Oncology, Giza, Egypt; 2 MISR International Hospital, General and GIT Surgery, Cairo, Egypt; 3 MISR Cancer Center, Medical Oncology, Giza, Egypt Background: Most patients with primary ovarian cancer develop a recurrence that is associated with a poor prognosis. Cytoreductive surgery (CRS) and hyperthermic intraperitoneal chemotherapy HIPEC have a promising results in patients with Recurrent Ovarian Cancer (ROC). Pathologic complete response (pCR) after HIPEC is an important prognosticator in the management of ROC. As rates of obesity is high among Egyptian Females, in this study it is clear that body mass index (BMI) affects the likelihood of achieving pCR and complete CRS. We describe an analysis of the relationship between BMI at diagnosis and pCR (absence of residual or recurrent disease) in ROC cases. With unfavorable levels of complete CRS, another important predictor of management outcomes in patients with ROC. Methods: 39 Study participants were ROC presented to our center from 7/2012 to 11/2015 of which 28 patients underwent the primary surgery at MISR Cancer Center and 11 are referred from other centers. As part of a prospective trial on residual tumor and whose postoperative pathological review definitively described pCR. Clinical characteristics potentially associated with pCR including BMI, age, race, residual tumor size/biomarkers, nodal status, morbidity issues(fistulas and Chest complications) and the presence of extra-pelvic metastasis were examined, as were the relationships between BMI, pCR, and residual tumors. We reported proportions, adjusted odds ratios (OR), and 95% confidence intervals (CI) significant at 2-tailed p < 005. Results: Of 39 patients, 24 (61%) had pCR; 27 (69%) were obese (BMI 30), and 15 (38%) had residual tumor. There was a difference between obese and non-obese (BMI <30) patients’ pCR rates(61% [24/39] vs. 38% [15/39], p = 0.69) or residual tumor(38% [15/39] vs. 61% [24/39],