- Email: [email protected]
Stereoselective biocatalysis in green solvents
Abstracts / Journal of Biotechnology 256S (2017) S44–S116
evaluated by microdilution, to the best of our knowledge for the first time. Acknowledgments: This work was supported by Anadolu University Scientific Research Projects (BAP-1401S008) which is part of the PhD thesis of ZF. http://dx.doi.org/10.1016/j.jbiotec.2017.06.976
S53
rolls was successfully used in continuous biocatalyst reactor for hydrolysis of lactose. http://dx.doi.org/10.1016/j.jbiotec.2017.06.978 Bioinformatics/System Biology Open-source bioinformatics software
Stereoselective biocatalysis in green solvents Manuela Panic ∗ , Petra Marin, Dora Hrestak, Martina Rajn, Valentina FiCko, Ivana RadojCic Redovnikovic, Marina Cvjetko Bubalo Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia E-mail address: [email protected] (M. Panic). The introduction of green chemistry principles into the preparation of various commercial products, such as chiral building blocks for drug preparation, is becoming an imperative in the scientific community. There are numerous tools of green chemistry such as the use of alternative solvents and environmentally benign raw (or renewable) materials and performing reactions by (bio)catalysis. The aim of this study is the application of two biocatalysts, purified lipase enzymes and whole-cell biocatalysis (plant cells), for the preparation of interesting chiral intermediates within environmentally friendly solvents (natural deep eutectic solvents, NADESs). For that purpose, enantioselective hydrolysis of (R, S)-1-phenylethyl acetate by immobilized Candida antarctica lipase B and Daucus carota root in cholinium-based NADESs as solvents, was conducted. Based on the results, we can conclude that for both biocatalysts the NADESs are superior to the traditional solvents when used in enantioselective hydrolysis of (R, S)-1-phenylethyl acetate, in terms of product yield and the enantiomeric excess (e.e.). Finally, if we consider environmental benefits of applying NADESs as solvents, they are excellent candidates for environment-friendly biocatalysis. http://dx.doi.org/10.1016/j.jbiotec.2017.06.977 Immobilization of beta-galactosidase on glass cloth rolls – Efficiency study
Constantin Vlagioiu 1,∗ , Vlad Vuta 2 , Florica Barbuceanu 2 , Gabriel Predoi 1 , Nicolae Tudor 1 1
Faculty of Veterinary Medicine, Bucharest, Romania Institute for Diagnosis and Animal Health, Bucharest, Romania 2
E-mail address: [email protected] (C. Vlagioiu). Copyright is the most common method used to protect the computer software and is legalized in most countries. The harmonization of the legislation is according to Bern Convention from 9th of September 1886, and Council Directive 91/25/CEE, transposed into Romanian law no. 8/1996. In this respect, this paper presents few of most used open-source software in biomedical research but not only, such as: Apache OpenOffice-platform for word processing, spreadsheets, presentations, graphics, databases; GIMP-for image manipulation program; GENtle software for viewing and editing DNA and amino acid sequences, integrated alignment, PCR, BLAST searches; Image J-an open source image processing program designed for scientific multidimensional images; R-a free software environment for statistical computing and graphics; QGIS-a free and open source Geographic Information System. The greatest advantage is no cost of ownership of open source software. The absence of licensing fees and maintenance fees are the major attraction. Other important advantages are: very large support you can get from the users community, it is no need to think to anti-piracy measures like product activation or serial number, the open source software continually evolving in real time, which means increased quality. Finally, the Open Source gives you a boundless freedom for your research. http://dx.doi.org/10.1016/j.jbiotec.2017.06.979
Faculty of Chemistry and Chemical Engineering, University of Maribor, Maribor, Slovenia
Developing an in silico analysis portal for unrevealing genomic signature of Archaic DNA that are related to modern human genetic diseases
E-mail address: [email protected] (D. Pecar).
Niyazi Senturk 1,∗ , Mahmut Cerkez Ergoren 2
In this study, we developed a method for -galactosidase immobilization using special glass cloth rolls as a new designed support. The effectiveness of glass cloth as a fibrous matrix with high porosity and open structure was explored. The new procedure provides simple, inexpensive and industrially applicable method and also support for active and stable immobilized biocatalyst. Glutaraldehyde was used as a non-specific cross-linking agent for fixing the -galactosidase on the glass cloth. Enzyme was covalently bonded on modified support. We tested the efficiency of bonding performing well-known hydrolysis of lactose. All experiments were carried out in the laboratory continuous reactor. The influence of reaction temperature (20, 25 and 30 ◦ C), substrate flow rate (1, 2 and 3 mL/min) and pH (6, 7, 8) on reaction conversion were studied. Reaction efficiency was monitored by measuring the glucose concentration using spectrophotometer. We reached high immobilization efficiency and final enzyme activity. The optimum reaction temperature, substrate flow rate and pH were found. -galactosidase immobilized on glass cloth
1 Department of Biomedical Engineering, Faculty of Engineering, Near East University, Nicosia, Cyprus 2 Department of Medical Biology, Medical Faculty, Near East University, Nicosia, Cyprus
Darja Pecar ∗ , Katja Zecevic, Andreja Gorsek
E-mail address: [email protected] (N. Senturk). During the history of human evolution, pathogens and the diseases they cause have been among the most significant selective forces experienced. Mutations or introgression can cause and rise adaptive allele up which some can be beneficial alleles. Archaic humans lived in Europe and Western Asia for more than 200,000 years. They were probably well adapted to this environment and its local pathogens. It is therefore conceivable that modern humans entering Europe and Western Asia who admixed with them obtained a substantial immune advantage from the introgression of archaic alleles. First aim of the study is to determine the genetic disease caused alleles that are intogressed from Archaics. Secondly, we designed the in-silico modelling (http://www.archaics2phenotype.
evaluated by microdilution, to the best of our knowledge for the first time. Acknowledgments: This work was supported by Anadolu University Scientific Research Projects (BAP-1401S008) which is part of the PhD thesis of ZF. http://dx.doi.org/10.1016/j.jbiotec.2017.06.976
S53
rolls was successfully used in continuous biocatalyst reactor for hydrolysis of lactose. http://dx.doi.org/10.1016/j.jbiotec.2017.06.978 Bioinformatics/System Biology Open-source bioinformatics software
Stereoselective biocatalysis in green solvents Manuela Panic ∗ , Petra Marin, Dora Hrestak, Martina Rajn, Valentina FiCko, Ivana RadojCic Redovnikovic, Marina Cvjetko Bubalo Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia E-mail address: [email protected] (M. Panic). The introduction of green chemistry principles into the preparation of various commercial products, such as chiral building blocks for drug preparation, is becoming an imperative in the scientific community. There are numerous tools of green chemistry such as the use of alternative solvents and environmentally benign raw (or renewable) materials and performing reactions by (bio)catalysis. The aim of this study is the application of two biocatalysts, purified lipase enzymes and whole-cell biocatalysis (plant cells), for the preparation of interesting chiral intermediates within environmentally friendly solvents (natural deep eutectic solvents, NADESs). For that purpose, enantioselective hydrolysis of (R, S)-1-phenylethyl acetate by immobilized Candida antarctica lipase B and Daucus carota root in cholinium-based NADESs as solvents, was conducted. Based on the results, we can conclude that for both biocatalysts the NADESs are superior to the traditional solvents when used in enantioselective hydrolysis of (R, S)-1-phenylethyl acetate, in terms of product yield and the enantiomeric excess (e.e.). Finally, if we consider environmental benefits of applying NADESs as solvents, they are excellent candidates for environment-friendly biocatalysis. http://dx.doi.org/10.1016/j.jbiotec.2017.06.977 Immobilization of beta-galactosidase on glass cloth rolls – Efficiency study
Constantin Vlagioiu 1,∗ , Vlad Vuta 2 , Florica Barbuceanu 2 , Gabriel Predoi 1 , Nicolae Tudor 1 1
Faculty of Veterinary Medicine, Bucharest, Romania Institute for Diagnosis and Animal Health, Bucharest, Romania 2
E-mail address: [email protected] (C. Vlagioiu). Copyright is the most common method used to protect the computer software and is legalized in most countries. The harmonization of the legislation is according to Bern Convention from 9th of September 1886, and Council Directive 91/25/CEE, transposed into Romanian law no. 8/1996. In this respect, this paper presents few of most used open-source software in biomedical research but not only, such as: Apache OpenOffice-platform for word processing, spreadsheets, presentations, graphics, databases; GIMP-for image manipulation program; GENtle software for viewing and editing DNA and amino acid sequences, integrated alignment, PCR, BLAST searches; Image J-an open source image processing program designed for scientific multidimensional images; R-a free software environment for statistical computing and graphics; QGIS-a free and open source Geographic Information System. The greatest advantage is no cost of ownership of open source software. The absence of licensing fees and maintenance fees are the major attraction. Other important advantages are: very large support you can get from the users community, it is no need to think to anti-piracy measures like product activation or serial number, the open source software continually evolving in real time, which means increased quality. Finally, the Open Source gives you a boundless freedom for your research. http://dx.doi.org/10.1016/j.jbiotec.2017.06.979
Faculty of Chemistry and Chemical Engineering, University of Maribor, Maribor, Slovenia
Developing an in silico analysis portal for unrevealing genomic signature of Archaic DNA that are related to modern human genetic diseases
E-mail address: [email protected] (D. Pecar).
Niyazi Senturk 1,∗ , Mahmut Cerkez Ergoren 2
In this study, we developed a method for -galactosidase immobilization using special glass cloth rolls as a new designed support. The effectiveness of glass cloth as a fibrous matrix with high porosity and open structure was explored. The new procedure provides simple, inexpensive and industrially applicable method and also support for active and stable immobilized biocatalyst. Glutaraldehyde was used as a non-specific cross-linking agent for fixing the -galactosidase on the glass cloth. Enzyme was covalently bonded on modified support. We tested the efficiency of bonding performing well-known hydrolysis of lactose. All experiments were carried out in the laboratory continuous reactor. The influence of reaction temperature (20, 25 and 30 ◦ C), substrate flow rate (1, 2 and 3 mL/min) and pH (6, 7, 8) on reaction conversion were studied. Reaction efficiency was monitored by measuring the glucose concentration using spectrophotometer. We reached high immobilization efficiency and final enzyme activity. The optimum reaction temperature, substrate flow rate and pH were found. -galactosidase immobilized on glass cloth
1 Department of Biomedical Engineering, Faculty of Engineering, Near East University, Nicosia, Cyprus 2 Department of Medical Biology, Medical Faculty, Near East University, Nicosia, Cyprus
Darja Pecar ∗ , Katja Zecevic, Andreja Gorsek
E-mail address: [email protected] (N. Senturk). During the history of human evolution, pathogens and the diseases they cause have been among the most significant selective forces experienced. Mutations or introgression can cause and rise adaptive allele up which some can be beneficial alleles. Archaic humans lived in Europe and Western Asia for more than 200,000 years. They were probably well adapted to this environment and its local pathogens. It is therefore conceivable that modern humans entering Europe and Western Asia who admixed with them obtained a substantial immune advantage from the introgression of archaic alleles. First aim of the study is to determine the genetic disease caused alleles that are intogressed from Archaics. Secondly, we designed the in-silico modelling (http://www.archaics2phenotype.