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STR data for 09 autosomal STR markers from Rio Grande do Sul (southern Brazil)
Forensic Science International 132 (2003) 223–224
Announcement of population data
STR data for 09 autosomal STR markers from Rio Grande do Sul (southern Brazil) Fa´bio P.N. Leite*, Felipe J. Menegassi, Solange P. Schwengber, Paulo E. Raimann, Trı´cia K. Albuquerque Setor de Biologia Molecular, Laborato´rio de Perı´cias, Instituto Geral de Perı´cias, Porto Alegre, Brazil Received 23 November 2002; accepted 2 January 2003
Abstract Allele frequencies for 09 STR autosomal loci (D3S1358, D5S818, D7S820, D8S1179, D13S317, D18S51, D21S11, FGA and VWA) included in the AmpF‘STR1 Profiler PlusTM were obtained from a sample of unrelated individuals from Rio Grande do Sul (southern Brazil). # 2003 Published by Elsevier Science Ireland Ltd. Keywords: Population data; STR; Profiler plusTM; Brazilian population
Population: Unrelated healthy volunteers donors from the region. Extraction: Phenol chloroform procedure [1].
Results: See Table 1. Analysis of data: GENEPOP [2].
PCR: 0.125 ng/ml target DNA, according to the manufacturer’s instruction (AmpF‘STR1 Profiler PlusTM, PE Applied Biosystems).
Other remarks: Four rare alleles, which were not detected in a previous study of the Brazilian population [3], were observed: allele 16.1 (locus FGA), allele 15.2 (locus vWA), allele 34.2 (locus D21S11) and allele 15.2 (locus D3S1358).
Typing: Capillary sequencer ABI 310 and reference sequenced ladders (PE Applied Biosystems).
This paper follows the guidelines for publication of population data requested by the journal [4].
* Corresponding author. Tel.: þ55-51-32882664; fax: þ55-51-32882667. E-mail address: [email protected] (F.P.N. Leite).
0379-0738/03/$ – see front matter # 2003 Published by Elsevier Science Ireland Ltd. doi:10.1016/S0379-0738(03)00017-3
224
F.P.N. Leite et al. / Forensic Science International 132 (2003) 223–224
Table 1 Allele frequencies and statistical parameters in a population sample from Rio Grande do Sul, Brazil Allelea 7 8 9 10 11 12 13 13.2 14 15 15.2 16 16.1 17 18 19 19.2 20 20.2 21 21.2 22 23 24 25 26 27 28 29 30 31 31.2 32.2 33.2 34 34.2 Hex Hob PD CE P
D3 (N: 104)
vWA (N: 103)
FGA (N: 101)
0.1827 0.1346 0.0048
0.2767 0.1602 0.0534 0.0146
0.7795 0.7788 0.9148 0.5603 0.0819
0.8012 0.7864 0.9302 0.574 0.0065
D18 (N: 93)
0.1971 0.149
0.0161 0.0054 0.1183 0.1237 0.0054 0.1828 0.1237
0.0198 0.005 0.005 0.005 0.0248 0.005 0.104 0.005 0.1881 0.0099 0.203 0.1238 0.1386 0.0842 0.0545 0.0149 0.005
0.0144
0.1398
0.0048
0.1237 0.0699 0.591
0.8708 0.7723 0.9681 0.5486 0.1185
0.8132 0.8077 0.9396 0.6134 0.0151
0.0096 0.0096 0.0583 0.1408 0.0097 0.2718
D21 (N: 102)
0.0288 0.101 0.0865 0.101 0.3173
0.0146
0.0769 0.2788 0.0048 0.2981
D8 (N: 104)
D5 (N: 104)
D13 (N: 104)
D7 (N: 98)
0.0144 0.0096 0.0481 0.0865 0.3702 0.3365 0.1058
0.101 0.0962 0.0577 0.2596 0.274 0.1731
0.0102 0.1582 0.1531 0.2806 0.2041 0.1633 0.0255
0.0192
0.0385
0.0051
0.8072 0.8462 0.9338 0.6874 0.6952
0.8078 0.7959 0.9327 0.4786 0.4406
0.0096
0.0323
0.0147 0.1618 0.1863 0.2059 0.0931 0.1324 0.098 0.392 0.098 0.0049 0.8616 0.8529 0.8447 0.7005 0.1011
0.8822 0.8817 0.6856 0.7582 0.1080
0.7315 0.7115 0.8837 0.4462 0.0014
a
Hex: expected heterozygosity; Hob: observed heterozygosity; PD: power of discrimination; CE: a priori chance of exclusion. P: Hardy Weinberg equilibrium, exact test.
References [1] J. Sambrook, E.F. Fritsch, T. Maniantis, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, 1989. [2] M. Raymond, F. Rousset, GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism, J. Heredity 86 (1995) 248–249.
[3] D. Grattapaglia, A.B. Schimidt, C. Costa e Silva, C. Stringher, A.P. Fernandes, M.E. Ferreira, Brazilian population data base for the 13 STR loci of AmpFlSTR1 Profiler PlusTM multiplex kits, Forensic Sci. Int. 118 (2001) 91–94. [4] P. Lincoln, A. Carracedo, Publication of population data of human polymorphisms, Forensic Sci. Int. 110 (2000) 3–5.
Announcement of population data
STR data for 09 autosomal STR markers from Rio Grande do Sul (southern Brazil) Fa´bio P.N. Leite*, Felipe J. Menegassi, Solange P. Schwengber, Paulo E. Raimann, Trı´cia K. Albuquerque Setor de Biologia Molecular, Laborato´rio de Perı´cias, Instituto Geral de Perı´cias, Porto Alegre, Brazil Received 23 November 2002; accepted 2 January 2003
Abstract Allele frequencies for 09 STR autosomal loci (D3S1358, D5S818, D7S820, D8S1179, D13S317, D18S51, D21S11, FGA and VWA) included in the AmpF‘STR1 Profiler PlusTM were obtained from a sample of unrelated individuals from Rio Grande do Sul (southern Brazil). # 2003 Published by Elsevier Science Ireland Ltd. Keywords: Population data; STR; Profiler plusTM; Brazilian population
Population: Unrelated healthy volunteers donors from the region. Extraction: Phenol chloroform procedure [1].
Results: See Table 1. Analysis of data: GENEPOP [2].
PCR: 0.125 ng/ml target DNA, according to the manufacturer’s instruction (AmpF‘STR1 Profiler PlusTM, PE Applied Biosystems).
Other remarks: Four rare alleles, which were not detected in a previous study of the Brazilian population [3], were observed: allele 16.1 (locus FGA), allele 15.2 (locus vWA), allele 34.2 (locus D21S11) and allele 15.2 (locus D3S1358).
Typing: Capillary sequencer ABI 310 and reference sequenced ladders (PE Applied Biosystems).
This paper follows the guidelines for publication of population data requested by the journal [4].
* Corresponding author. Tel.: þ55-51-32882664; fax: þ55-51-32882667. E-mail address: [email protected] (F.P.N. Leite).
0379-0738/03/$ – see front matter # 2003 Published by Elsevier Science Ireland Ltd. doi:10.1016/S0379-0738(03)00017-3
224
F.P.N. Leite et al. / Forensic Science International 132 (2003) 223–224
Table 1 Allele frequencies and statistical parameters in a population sample from Rio Grande do Sul, Brazil Allelea 7 8 9 10 11 12 13 13.2 14 15 15.2 16 16.1 17 18 19 19.2 20 20.2 21 21.2 22 23 24 25 26 27 28 29 30 31 31.2 32.2 33.2 34 34.2 Hex Hob PD CE P
D3 (N: 104)
vWA (N: 103)
FGA (N: 101)
0.1827 0.1346 0.0048
0.2767 0.1602 0.0534 0.0146
0.7795 0.7788 0.9148 0.5603 0.0819
0.8012 0.7864 0.9302 0.574 0.0065
D18 (N: 93)
0.1971 0.149
0.0161 0.0054 0.1183 0.1237 0.0054 0.1828 0.1237
0.0198 0.005 0.005 0.005 0.0248 0.005 0.104 0.005 0.1881 0.0099 0.203 0.1238 0.1386 0.0842 0.0545 0.0149 0.005
0.0144
0.1398
0.0048
0.1237 0.0699 0.591
0.8708 0.7723 0.9681 0.5486 0.1185
0.8132 0.8077 0.9396 0.6134 0.0151
0.0096 0.0096 0.0583 0.1408 0.0097 0.2718
D21 (N: 102)
0.0288 0.101 0.0865 0.101 0.3173
0.0146
0.0769 0.2788 0.0048 0.2981
D8 (N: 104)
D5 (N: 104)
D13 (N: 104)
D7 (N: 98)
0.0144 0.0096 0.0481 0.0865 0.3702 0.3365 0.1058
0.101 0.0962 0.0577 0.2596 0.274 0.1731
0.0102 0.1582 0.1531 0.2806 0.2041 0.1633 0.0255
0.0192
0.0385
0.0051
0.8072 0.8462 0.9338 0.6874 0.6952
0.8078 0.7959 0.9327 0.4786 0.4406
0.0096
0.0323
0.0147 0.1618 0.1863 0.2059 0.0931 0.1324 0.098 0.392 0.098 0.0049 0.8616 0.8529 0.8447 0.7005 0.1011
0.8822 0.8817 0.6856 0.7582 0.1080
0.7315 0.7115 0.8837 0.4462 0.0014
a
Hex: expected heterozygosity; Hob: observed heterozygosity; PD: power of discrimination; CE: a priori chance of exclusion. P: Hardy Weinberg equilibrium, exact test.
References [1] J. Sambrook, E.F. Fritsch, T. Maniantis, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, 1989. [2] M. Raymond, F. Rousset, GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism, J. Heredity 86 (1995) 248–249.
[3] D. Grattapaglia, A.B. Schimidt, C. Costa e Silva, C. Stringher, A.P. Fernandes, M.E. Ferreira, Brazilian population data base for the 13 STR loci of AmpFlSTR1 Profiler PlusTM multiplex kits, Forensic Sci. Int. 118 (2001) 91–94. [4] P. Lincoln, A. Carracedo, Publication of population data of human polymorphisms, Forensic Sci. Int. 110 (2000) 3–5.