- Email: [email protected]
STR data for the AmpFlSTR profiler plus loci from Majorcan, Minorcan and Valencian populations (Eastern Spain)
Forensic Science International 121 (2001) 201±204
Announcement of population data
STR data for the AmpFlSTR pro®ler plus loci from Majorcan, Minorcan and Valencian populations (Eastern Spain) Carmen TomaÁs, AntoÁnia Picornell*, Jose A. Castro, Ma. MisericoÁrdia Laboratori de GeneÁtica, Departament de Biologia, Universitat de les Illes Balears, Crta. Valldemossa, km 7.5. 07071-Palma de Mallorca, Balears, Spain Received 15 December 2000; received in revised form 10 January 2001; accepted 10 January 2001
Abstract Frequency data of the nine STRs included in the AmpFlSTR pro®ler plus kit were determined in a sample of 304 unrelated individuals from three Spanish populations: 103 from Majorca, 100 from Minorca, and 101 from Valencia. # 2001 Elsevier Science Ireland Ltd. All rights reserved. Keywords: Short tandem repeats; AmpFlSTR pro®ler plus; Population data; Eastern Spain
Population: Autochthonous unrelated individuals from Valencia and the islands of Majorca and Minorca. Extraction: Standard phenol/chloroform DNA extraction [1]. PCR: The co-ampli®cation of these loci was performed following the manufacturer's instructions, using approximately 2 ng of genomic DNA in a total reaction volume of 10 ml. Typing: ABI 310 and reference sequenced ladders (PE Applied Biosystems). Results: See Tables 1 and 2. Analysis of data: GENEPOP [2], BIOSYS-1 [3] packages. Other remarks: No deviations from Hardy±Weinberg equilibrium were observed. Comparisons between all of the
three populations studied showed no signi®cant differences, with the exception of the FGA system (P < 0:001). FST values between these populations and other Spanish and Portuguese populations showed only signi®cant differences between Basques and Minorcans. No evidence of signi®cant allele association was observed in the pairwise comparisons between loci. The AmpFlSTR pro®ler plus was used in 26 paternity investigation cases, previously tested with other loci. Ten were exclusion cases (38.5%). Exclusions in between two and seven loci were observed, eight of them (80%) were con®rmed in at least four loci. All the cases showing no exclusion paternity had probability of paternity > 99:95% (PI > 2000), which is above the limit usually accepted by some courts for attribution of paternity [4].
* Corresponding author. Tel.: 34-971-173332; fax: 34-971-173184. E-mail address: [email protected] (A. Picornell).
0379-0738/01/$ ± see front matter # 2001 Elsevier Science Ireland Ltd. All rights reserved. PII: S 0 3 7 9 - 0 7 3 8 ( 0 1 ) 0 0 4 0 2 - 9
C. TomaÁs et al. / Forensic Science International 121 (2001) 201±204
202
Table 1 Allele frequencies of the STRs D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317 and D7S820 in the populations studied (number of individuals in parenthesis) Locus D3S1358
vWA
FGA
D8S1179
D21S11
Allele
Majorca (103)
Minorca (100)
Valencia (101)
Pooled (304)
11 13 14 15 16 17 18 19
± ± 0.117 0.199 0.296 0.160 0.218 0.010
0.005 ± 0.095 0.190 0.295 0.245 0.165 0.005
± 0.005 0.109 0.223 0.262 0.203 0.178 0.020
0.002 0.002 0.107 0.204 0.285 0.202 0.188 0.012
11 13 14 15 16 17 18 19 20
0.005 0.005 0.102 0.102 0.218 0.296 0.189 0.068 0.015
± ± 0.100 0.090 0.305 0.230 0.185 0.090 ±
± ± 0.119 0.134 0.248 0.282 0.139 0.064 0.015
0.002 0.002 0.107 0.109 0.257 0.270 0.171 0.074 0.010
18 19 20 21 21.2 22 22.2 23 23.2 24 24.2 25 26 27 28
0.015 0.087 0.117 0.252 ± 0.175 0.010 0.087 0.005 0.102 ± 0.112 0.034 ± 0.005
± 0.075 0.185 0.145 ± 0.195 0.030 0.125 ± 0.140 ± 0.055 0.045 0.005 ±
0.025 0.069 0.158 0.183 0.010 0.109 ± 0.119 ± 0.158 0.005 0.129 0.025 0.010 ±
0.013 0.077 0.153 0.194 0.003 0.160 0.013 0.110 0.002 0.133 0.002 0.099 0.035 0.005 0.002
7 8 9 10 11 12 13 14 15 16 17
0.005 0.005 ± 0.093 0.098 0.127 0.314 0.186 0.118 0.044 0.010
± 0.030 ± 0.160 0.115 0.110 0.305 0.135 0.125 0.020 ±
± 0.010 0.015 0.089 0.099 0.139 0.297 0.168 0.153 0.030 ±
0.002 0.015 0.005 0.114 0.104 0.125 0.305 0.163 0.132 0.031 0.003
24.2 26 27 28 29 30
0.005 0.010 0.015 0.109 0.243 0.297
± ± 0.015 0.195 0.235 0.195
± ± 0.025 0.124 0.203 0.267
0.002 0.003 0.018 0.142 0.227 0.253
C. TomaÁs et al. / Forensic Science International 121 (2001) 201±204
203
Table 1 (Continued ) Locus
D18S51
D5S818
D13S317
D7S820
Allele
Majorca (103)
Minorca (100)
Valencia (101)
Pooled (304)
30.2 31 31.2 32 32.2 33.2 34 34.2
0.035 0.059 0.079 0.015 0.099 0.025 0.005 0.005
0.055 0.065 0.095 0.010 0.095 0.030 ± 0.010
0.050 0.064 0.099 0.005 0.139 0.020 ± 0.005
0.046 0.063 0.091 0.010 0.111 0.025 0.002 0.007
9 10 11 12 13 14 15 16 17 18 19 20 21 22
0.010 ± 0.025 0.088 0.108 0.172 0.118 0.181 0.157 0.059 0.039 0.025 0.020 ±
0.005 0.005 0.010 0.145 0.160 0.130 0.160 0.100 0.125 0.085 0.050 0.015 0.005 0.005
± 0.005 0.005 0.168 0.104 0.144 0.139 0.158 0.119 0.089 0.054 0.010 0.005 ±
0.005 0.003 0.013 0.134 0.124 0.149 0.139 0.147 0.134 0.078 0.048 0.017 0.010 0.002
8 9 10 11 12 13 14
± 0.034 0.121 0.320 0.364 0.146 0.015
± 0.025 0.120 0.360 0.370 0.120 0.005
0.015 0.020 0.089 0.347 0.396 0.124 0.010
0.005 0.026 0.110 0.342 0.377 0.130 0.010
8 9 10 11 12 13 14
0.121 0.068 0.058 0.291 0.359 0.073 0.029
0.095 0.065 0.055 0.255 0.375 0.120 0.035
0.144 0.079 0.040 0.347 0.252 0.094 0.045
0.120 0.071 0.051 0.298 0.329 0.095 0.036
7 8 9 10 11 12 13 14
0.029 0.214 0.121 0.316 0.189 0.102 0.019 0.010
0.025 0.130 0.115 0.225 0.280 0.185 0.040 ±
0.015 0.153 0.114 0.287 0.228 0.173 0.020 0.010
0.023 0.166 0.117 0.276 0.232 0.153 0.026 0.007
C. TomaÁs et al. / Forensic Science International 121 (2001) 201±204
204
Table 2 Statistical parameters for the STRs studied in the pooled populationsa System
Number of alleles
H (observed/ expected)
Deficiency of heterozygotes
P-exact test
PD
CE
CE2
D3S1358 vWA FGA D8S1179 D21S11 D18S51 D5S818 D13S317 D7S820 Average
8 9 15 11 14 14 7 7 8 10.3
0.780/0.791 0.806/0.805 0.885/0.868 0.812/0.823 0.808/0.838 0.861/0.879 0.701/0.712 0.793/0.772 0.789/0.805 0.804/0.810
0.278 0.651 0.247 0.517 0.261 0.470 0.411 0.834 0.344 ±
0.818 0.274 0.068 0.590 0.090 0.322 0.851 0.250 0.881 0.342
0.923 0.933 0.967 0.947 0.954 0.972 0.867 0.915 0.933 1/1.137 E11
0.582 0.613 0.730 0.654 0.679 0.749 0.468 0.567 0.613 0.99989
0.403 0.435 0.572 0.480 0.510 0.596 0.340 0.389 0.436 0.99662
a H: heterozygosity; PD: power of discrimination; CE: chance of exclusion; CE2: chance of exclusion if only one parent and child are typed.
Acknowledgements This work was supported by grant PM97-0041 from the DireccioÂn General de EnsenÄanza Superior (Spain). C.T. is a recipient of a F.P.I. fellowship from the Ministerio de EduacioÂn y Cultura (Spain). References [1] J. Sambrook, E.F. Fritsch, T. Maniatis, Molecular cloning: a laboratory manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, 1989.
[2] M. Raymond, F. Rousset, GENEPOP (Version 1.2): population genetics software for exact tests and ecumenicism, J. Heredity 86 (1995) 248±249. [3] D.L. Swofford, R.B. Selander, BIOSYS-1. A Computer Program for the Analysis of Allelic Variation in Population Genetics and Biochemical Systematics, Release 1.7, University of Illinois, Urbana, IL, 1989. [4] R. Domenici, R. Bibbiani, S. Fornaciari, M.A. Nardone, I. Rocchi, M. Spinetti, M. Venturi, M. Bargagna, Application of conventional and DNA polymorphism to paternity cases, in: B. Olaisen, B. Brikmann, P. Lincolln (Eds.), Progress in Forensic Genetics, Vol. 7, Excerpta Medica, Amsterdam, 1998, pp. 89±91.
Announcement of population data
STR data for the AmpFlSTR pro®ler plus loci from Majorcan, Minorcan and Valencian populations (Eastern Spain) Carmen TomaÁs, AntoÁnia Picornell*, Jose A. Castro, Ma. MisericoÁrdia Laboratori de GeneÁtica, Departament de Biologia, Universitat de les Illes Balears, Crta. Valldemossa, km 7.5. 07071-Palma de Mallorca, Balears, Spain Received 15 December 2000; received in revised form 10 January 2001; accepted 10 January 2001
Abstract Frequency data of the nine STRs included in the AmpFlSTR pro®ler plus kit were determined in a sample of 304 unrelated individuals from three Spanish populations: 103 from Majorca, 100 from Minorca, and 101 from Valencia. # 2001 Elsevier Science Ireland Ltd. All rights reserved. Keywords: Short tandem repeats; AmpFlSTR pro®ler plus; Population data; Eastern Spain
Population: Autochthonous unrelated individuals from Valencia and the islands of Majorca and Minorca. Extraction: Standard phenol/chloroform DNA extraction [1]. PCR: The co-ampli®cation of these loci was performed following the manufacturer's instructions, using approximately 2 ng of genomic DNA in a total reaction volume of 10 ml. Typing: ABI 310 and reference sequenced ladders (PE Applied Biosystems). Results: See Tables 1 and 2. Analysis of data: GENEPOP [2], BIOSYS-1 [3] packages. Other remarks: No deviations from Hardy±Weinberg equilibrium were observed. Comparisons between all of the
three populations studied showed no signi®cant differences, with the exception of the FGA system (P < 0:001). FST values between these populations and other Spanish and Portuguese populations showed only signi®cant differences between Basques and Minorcans. No evidence of signi®cant allele association was observed in the pairwise comparisons between loci. The AmpFlSTR pro®ler plus was used in 26 paternity investigation cases, previously tested with other loci. Ten were exclusion cases (38.5%). Exclusions in between two and seven loci were observed, eight of them (80%) were con®rmed in at least four loci. All the cases showing no exclusion paternity had probability of paternity > 99:95% (PI > 2000), which is above the limit usually accepted by some courts for attribution of paternity [4].
* Corresponding author. Tel.: 34-971-173332; fax: 34-971-173184. E-mail address: [email protected] (A. Picornell).
0379-0738/01/$ ± see front matter # 2001 Elsevier Science Ireland Ltd. All rights reserved. PII: S 0 3 7 9 - 0 7 3 8 ( 0 1 ) 0 0 4 0 2 - 9
C. TomaÁs et al. / Forensic Science International 121 (2001) 201±204
202
Table 1 Allele frequencies of the STRs D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317 and D7S820 in the populations studied (number of individuals in parenthesis) Locus D3S1358
vWA
FGA
D8S1179
D21S11
Allele
Majorca (103)
Minorca (100)
Valencia (101)
Pooled (304)
11 13 14 15 16 17 18 19
± ± 0.117 0.199 0.296 0.160 0.218 0.010
0.005 ± 0.095 0.190 0.295 0.245 0.165 0.005
± 0.005 0.109 0.223 0.262 0.203 0.178 0.020
0.002 0.002 0.107 0.204 0.285 0.202 0.188 0.012
11 13 14 15 16 17 18 19 20
0.005 0.005 0.102 0.102 0.218 0.296 0.189 0.068 0.015
± ± 0.100 0.090 0.305 0.230 0.185 0.090 ±
± ± 0.119 0.134 0.248 0.282 0.139 0.064 0.015
0.002 0.002 0.107 0.109 0.257 0.270 0.171 0.074 0.010
18 19 20 21 21.2 22 22.2 23 23.2 24 24.2 25 26 27 28
0.015 0.087 0.117 0.252 ± 0.175 0.010 0.087 0.005 0.102 ± 0.112 0.034 ± 0.005
± 0.075 0.185 0.145 ± 0.195 0.030 0.125 ± 0.140 ± 0.055 0.045 0.005 ±
0.025 0.069 0.158 0.183 0.010 0.109 ± 0.119 ± 0.158 0.005 0.129 0.025 0.010 ±
0.013 0.077 0.153 0.194 0.003 0.160 0.013 0.110 0.002 0.133 0.002 0.099 0.035 0.005 0.002
7 8 9 10 11 12 13 14 15 16 17
0.005 0.005 ± 0.093 0.098 0.127 0.314 0.186 0.118 0.044 0.010
± 0.030 ± 0.160 0.115 0.110 0.305 0.135 0.125 0.020 ±
± 0.010 0.015 0.089 0.099 0.139 0.297 0.168 0.153 0.030 ±
0.002 0.015 0.005 0.114 0.104 0.125 0.305 0.163 0.132 0.031 0.003
24.2 26 27 28 29 30
0.005 0.010 0.015 0.109 0.243 0.297
± ± 0.015 0.195 0.235 0.195
± ± 0.025 0.124 0.203 0.267
0.002 0.003 0.018 0.142 0.227 0.253
C. TomaÁs et al. / Forensic Science International 121 (2001) 201±204
203
Table 1 (Continued ) Locus
D18S51
D5S818
D13S317
D7S820
Allele
Majorca (103)
Minorca (100)
Valencia (101)
Pooled (304)
30.2 31 31.2 32 32.2 33.2 34 34.2
0.035 0.059 0.079 0.015 0.099 0.025 0.005 0.005
0.055 0.065 0.095 0.010 0.095 0.030 ± 0.010
0.050 0.064 0.099 0.005 0.139 0.020 ± 0.005
0.046 0.063 0.091 0.010 0.111 0.025 0.002 0.007
9 10 11 12 13 14 15 16 17 18 19 20 21 22
0.010 ± 0.025 0.088 0.108 0.172 0.118 0.181 0.157 0.059 0.039 0.025 0.020 ±
0.005 0.005 0.010 0.145 0.160 0.130 0.160 0.100 0.125 0.085 0.050 0.015 0.005 0.005
± 0.005 0.005 0.168 0.104 0.144 0.139 0.158 0.119 0.089 0.054 0.010 0.005 ±
0.005 0.003 0.013 0.134 0.124 0.149 0.139 0.147 0.134 0.078 0.048 0.017 0.010 0.002
8 9 10 11 12 13 14
± 0.034 0.121 0.320 0.364 0.146 0.015
± 0.025 0.120 0.360 0.370 0.120 0.005
0.015 0.020 0.089 0.347 0.396 0.124 0.010
0.005 0.026 0.110 0.342 0.377 0.130 0.010
8 9 10 11 12 13 14
0.121 0.068 0.058 0.291 0.359 0.073 0.029
0.095 0.065 0.055 0.255 0.375 0.120 0.035
0.144 0.079 0.040 0.347 0.252 0.094 0.045
0.120 0.071 0.051 0.298 0.329 0.095 0.036
7 8 9 10 11 12 13 14
0.029 0.214 0.121 0.316 0.189 0.102 0.019 0.010
0.025 0.130 0.115 0.225 0.280 0.185 0.040 ±
0.015 0.153 0.114 0.287 0.228 0.173 0.020 0.010
0.023 0.166 0.117 0.276 0.232 0.153 0.026 0.007
C. TomaÁs et al. / Forensic Science International 121 (2001) 201±204
204
Table 2 Statistical parameters for the STRs studied in the pooled populationsa System
Number of alleles
H (observed/ expected)
Deficiency of heterozygotes
P-exact test
PD
CE
CE2
D3S1358 vWA FGA D8S1179 D21S11 D18S51 D5S818 D13S317 D7S820 Average
8 9 15 11 14 14 7 7 8 10.3
0.780/0.791 0.806/0.805 0.885/0.868 0.812/0.823 0.808/0.838 0.861/0.879 0.701/0.712 0.793/0.772 0.789/0.805 0.804/0.810
0.278 0.651 0.247 0.517 0.261 0.470 0.411 0.834 0.344 ±
0.818 0.274 0.068 0.590 0.090 0.322 0.851 0.250 0.881 0.342
0.923 0.933 0.967 0.947 0.954 0.972 0.867 0.915 0.933 1/1.137 E11
0.582 0.613 0.730 0.654 0.679 0.749 0.468 0.567 0.613 0.99989
0.403 0.435 0.572 0.480 0.510 0.596 0.340 0.389 0.436 0.99662
a H: heterozygosity; PD: power of discrimination; CE: chance of exclusion; CE2: chance of exclusion if only one parent and child are typed.
Acknowledgements This work was supported by grant PM97-0041 from the DireccioÂn General de EnsenÄanza Superior (Spain). C.T. is a recipient of a F.P.I. fellowship from the Ministerio de EduacioÂn y Cultura (Spain). References [1] J. Sambrook, E.F. Fritsch, T. Maniatis, Molecular cloning: a laboratory manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, 1989.
[2] M. Raymond, F. Rousset, GENEPOP (Version 1.2): population genetics software for exact tests and ecumenicism, J. Heredity 86 (1995) 248±249. [3] D.L. Swofford, R.B. Selander, BIOSYS-1. A Computer Program for the Analysis of Allelic Variation in Population Genetics and Biochemical Systematics, Release 1.7, University of Illinois, Urbana, IL, 1989. [4] R. Domenici, R. Bibbiani, S. Fornaciari, M.A. Nardone, I. Rocchi, M. Spinetti, M. Venturi, M. Bargagna, Application of conventional and DNA polymorphism to paternity cases, in: B. Olaisen, B. Brikmann, P. Lincolln (Eds.), Progress in Forensic Genetics, Vol. 7, Excerpta Medica, Amsterdam, 1998, pp. 89±91.