- Email: [email protected]
Structural Studies of Physiologically Active Polysaccharides of the Quinine Conk Fomitopsis Officinalis
Achievements in the Life Sciences 10 (2016) S13
Contents lists available at ScienceDirect
Achievements in the Life Sciences journal homepage: www.elsevier.com/locate/als
Abstracts
Structural Studies of Physiologically Active Polysaccharides of the Quinine Conk Fomitopsis Officinalis Victoria V. Golovchenko a,⁎, Olga A. Patova a, Dmitriy A. Latkin a, Naranmandakh Shinen b, Dagiisuren b, Ganbaatar Jamsranjav c, Alexander S. Shashkov d a
Institute of Physiology, Komi Science Centre, The Urals Branch RAS, Syktyvkar, Russia National University of Mongolia, School of Life Sciences, Ulaanbaatar-51, Mongolia c Institute of Chemistry and Chemical Technology, The Mongolian Academy of Sciences, Ulaanbaatar-51, Mongolia d N.D. Zelinsky Institute of Organic Chemistry RAS, Moscow, Russia b
Higher basidiomycetes mushrooms are a source of biologically active substances. The immunomodulating and antitumor effect of basidiomycetes mushrooms was connected with β-D-1,3-glucans and their protein derived peptides that basidiomycetes mushrooms produced. The quinine conk Fomitopsis officinalis (Vill.: Fr.) Bond. et Sing. is of interest because it was actively used in the folk medicine and its reserves are significant in Western Europe, North America, the Urals and Siberia. The aim of this work was to study the structure and cytostatic activity of three polysaccharides HWE, CA, HA obtained from fruiting body of quinine conk by the successive extraction with water at 100 °С, 4 h, and 5% aqueous sodium hydroxide at 4 °C, 24 h and 80 °C, 6 h, respectively. Polysaccharides HWE, CA, HA were differed by the content of the majore components (glucose, fucose, galactose and mannose) in carbohydrate chains. The carbohydrate chains of CA contain only the glucose residues, whereas carbohydrate chains of HWE and HA included different number of fucose, mannose and galactose residues and had similar structure. The carbohydrate chains of HWE included significant number (13%) of fucose residues. The data of NMR and GLC-MS determined that carbohydrate chains of glucan CA were characterized by the most simplest structure. Its carbohydrate chains contained 1,3-β-linked D-glucopyranose residues, and 3,6-substituted D-glucopyranose residues as branched points. Polysaccharides HWE and HA were characterized by the similar structure, their carbohydrate chains contained terminal, 1,3- and 1,6-linked β-D-glucopyranose residues, 1,3-linked α-D-mannopyranose residues, 1,3-linked α-L-fucopyranose residues. Polysaccharide HA included disaccharide fragments: α-L-Fucp(1 → 2)-β-Xylp-(1→), that attached to the 4-position of the 1,3-linked α-D-mannopyranose residues. Inhibitory effect of polysaccharides CA, HWE and HA was in vitro demonstrated on the growth of cervical epithelial cell line HeLa. The polysaccharides exhibited a dose-dependent activity within the concentration range of 20–300 μg/ml. Polysaccharide HA showed more antiproliferative activity than that of CA, HWE at all concentrations (р b 0,05). The inhibitory effect of HA was maximal (59%) at a concentration of 300 μg/ml.
⁎ Corresponding author. E-mail address: [email protected] (V.V. Golovchenko). Peer review under responsibility of Far Eastern Federal University.
http://dx.doi.org/10.1016/j.als.2016.12.013 2078-1520/© 2017 Far Eastern Federal University. Hosting by Elsevier B.V. All rights reserved.
Contents lists available at ScienceDirect
Achievements in the Life Sciences journal homepage: www.elsevier.com/locate/als
Abstracts
Structural Studies of Physiologically Active Polysaccharides of the Quinine Conk Fomitopsis Officinalis Victoria V. Golovchenko a,⁎, Olga A. Patova a, Dmitriy A. Latkin a, Naranmandakh Shinen b, Dagiisuren b, Ganbaatar Jamsranjav c, Alexander S. Shashkov d a
Institute of Physiology, Komi Science Centre, The Urals Branch RAS, Syktyvkar, Russia National University of Mongolia, School of Life Sciences, Ulaanbaatar-51, Mongolia c Institute of Chemistry and Chemical Technology, The Mongolian Academy of Sciences, Ulaanbaatar-51, Mongolia d N.D. Zelinsky Institute of Organic Chemistry RAS, Moscow, Russia b
Higher basidiomycetes mushrooms are a source of biologically active substances. The immunomodulating and antitumor effect of basidiomycetes mushrooms was connected with β-D-1,3-glucans and their protein derived peptides that basidiomycetes mushrooms produced. The quinine conk Fomitopsis officinalis (Vill.: Fr.) Bond. et Sing. is of interest because it was actively used in the folk medicine and its reserves are significant in Western Europe, North America, the Urals and Siberia. The aim of this work was to study the structure and cytostatic activity of three polysaccharides HWE, CA, HA obtained from fruiting body of quinine conk by the successive extraction with water at 100 °С, 4 h, and 5% aqueous sodium hydroxide at 4 °C, 24 h and 80 °C, 6 h, respectively. Polysaccharides HWE, CA, HA were differed by the content of the majore components (glucose, fucose, galactose and mannose) in carbohydrate chains. The carbohydrate chains of CA contain only the glucose residues, whereas carbohydrate chains of HWE and HA included different number of fucose, mannose and galactose residues and had similar structure. The carbohydrate chains of HWE included significant number (13%) of fucose residues. The data of NMR and GLC-MS determined that carbohydrate chains of glucan CA were characterized by the most simplest structure. Its carbohydrate chains contained 1,3-β-linked D-glucopyranose residues, and 3,6-substituted D-glucopyranose residues as branched points. Polysaccharides HWE and HA were characterized by the similar structure, their carbohydrate chains contained terminal, 1,3- and 1,6-linked β-D-glucopyranose residues, 1,3-linked α-D-mannopyranose residues, 1,3-linked α-L-fucopyranose residues. Polysaccharide HA included disaccharide fragments: α-L-Fucp(1 → 2)-β-Xylp-(1→), that attached to the 4-position of the 1,3-linked α-D-mannopyranose residues. Inhibitory effect of polysaccharides CA, HWE and HA was in vitro demonstrated on the growth of cervical epithelial cell line HeLa. The polysaccharides exhibited a dose-dependent activity within the concentration range of 20–300 μg/ml. Polysaccharide HA showed more antiproliferative activity than that of CA, HWE at all concentrations (р b 0,05). The inhibitory effect of HA was maximal (59%) at a concentration of 300 μg/ml.
⁎ Corresponding author. E-mail address: [email protected] (V.V. Golovchenko). Peer review under responsibility of Far Eastern Federal University.
http://dx.doi.org/10.1016/j.als.2016.12.013 2078-1520/© 2017 Far Eastern Federal University. Hosting by Elsevier B.V. All rights reserved.