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Structure and physiological activity of pectic polysaccharides of the top of the Mongolian onion Allium mongolicum
Achievements in the Life Sciences 10 (2016) S35
Contents lists available at ScienceDirect
Achievements in the Life Sciences journal homepage: www.elsevier.com/locate/als
Abstracts
Structure and physiological activity of pectic polysaccharides of the top of the Mongolian onion Allium mongolicum Olga A. Patova a, Victoria V. Golovchenko a,⁎, Nataliya Ya. Mikhaleva a, Naranmandakh Shinen b, Dagiisuren b, Ganbaatar Jamsranjav c, Alexander S. Shashkov d a
Institute of Physiology, Komi Science Centre, The Urals Branch RAS, Syktyvkar, Russia National University of Mongolia, School of Life Sciences, Ulaanbaatar-51, Mongolia c Institute of Chemistry and Chemical Technology, Mongolian Academy of Sciences, Ulaanbaatar-51, Mongolia d N.D.Zelinsky Institute of Organic Chemistry RAS, Moscow, Russia b
Alium mongolicum and Allium polyrhizum are the most common and important plants of Mongolian desert and steppe. The leaves of onion А. mongolicum and А. polyrhizum are widely used in human food and livestock feeding, compensating the lack of plant foods in these climates. This paper presents the results of the structure study and immunological activity of pectic polysaccharide isolated from the top of the Mongolian onion A. mongolicum using 0.7% aqueous ammonium oxalate. NMR-spectral data determined, that obtained polysaccharide was characterized by typical pectin structure and had a relatively high content of rhamnose residues. Due to the high degree of substitution of rhamnose residues of the backbone the signals of 1,4-linked β-D-Galp residues and 1,5-linked α-L-Araf residues of side chains were only determined in the NMR spectra. The pectin was subjected to a partial acid (0.05 M TFA) hydrolysis and controlled enzymatic (1,4-α-D-polygalacturonase with endoand exo-activity) hydrolysis for the structure study and substitution type of the backbone determination. In a result, the linear regions of galacturonan and the side chains were removed. The obtained polysaccharide fragment (26% yield), a regular ramnogalacturonan, was characterized by Rha and GalA ratio of 1:1. Such structure of the backbone provides a high concentration of side chains at C-4 position of rhamnose residues on a single repeating unit in the backbone of pectin. The presence of 2,4-di-O-substituted rhamnose residues, 3,4-diO-substituted and 1,4-linked arabinose residues, 1,3-, 1,6-linked and 3,4-, 3,6-, 4,6-di-O-substituted galactose or glucose residues in pectin of A. mongolicum were determined by chromato-mass spectrometry. Inhibitory effect of pectin from the top of the Mongolian onion A. mongolicum was in vitro demonstrated on the growth of cervical epithelial cell line HeLa. The pectin exhibited a dose-dependent activity.
⁎ Corresponding author. E-mail address: [email protected] (V.V. Golovchenko). Peer review under responsibility of Far Eastern Federal University.
http://dx.doi.org/10.1016/j.als.2016.12.034 2078-1520/© 2017 Far Eastern Federal University. Hosting by Elsevier B.V. All rights reserved.
Contents lists available at ScienceDirect
Achievements in the Life Sciences journal homepage: www.elsevier.com/locate/als
Abstracts
Structure and physiological activity of pectic polysaccharides of the top of the Mongolian onion Allium mongolicum Olga A. Patova a, Victoria V. Golovchenko a,⁎, Nataliya Ya. Mikhaleva a, Naranmandakh Shinen b, Dagiisuren b, Ganbaatar Jamsranjav c, Alexander S. Shashkov d a
Institute of Physiology, Komi Science Centre, The Urals Branch RAS, Syktyvkar, Russia National University of Mongolia, School of Life Sciences, Ulaanbaatar-51, Mongolia c Institute of Chemistry and Chemical Technology, Mongolian Academy of Sciences, Ulaanbaatar-51, Mongolia d N.D.Zelinsky Institute of Organic Chemistry RAS, Moscow, Russia b
Alium mongolicum and Allium polyrhizum are the most common and important plants of Mongolian desert and steppe. The leaves of onion А. mongolicum and А. polyrhizum are widely used in human food and livestock feeding, compensating the lack of plant foods in these climates. This paper presents the results of the structure study and immunological activity of pectic polysaccharide isolated from the top of the Mongolian onion A. mongolicum using 0.7% aqueous ammonium oxalate. NMR-spectral data determined, that obtained polysaccharide was characterized by typical pectin structure and had a relatively high content of rhamnose residues. Due to the high degree of substitution of rhamnose residues of the backbone the signals of 1,4-linked β-D-Galp residues and 1,5-linked α-L-Araf residues of side chains were only determined in the NMR spectra. The pectin was subjected to a partial acid (0.05 M TFA) hydrolysis and controlled enzymatic (1,4-α-D-polygalacturonase with endoand exo-activity) hydrolysis for the structure study and substitution type of the backbone determination. In a result, the linear regions of galacturonan and the side chains were removed. The obtained polysaccharide fragment (26% yield), a regular ramnogalacturonan, was characterized by Rha and GalA ratio of 1:1. Such structure of the backbone provides a high concentration of side chains at C-4 position of rhamnose residues on a single repeating unit in the backbone of pectin. The presence of 2,4-di-O-substituted rhamnose residues, 3,4-diO-substituted and 1,4-linked arabinose residues, 1,3-, 1,6-linked and 3,4-, 3,6-, 4,6-di-O-substituted galactose or glucose residues in pectin of A. mongolicum were determined by chromato-mass spectrometry. Inhibitory effect of pectin from the top of the Mongolian onion A. mongolicum was in vitro demonstrated on the growth of cervical epithelial cell line HeLa. The pectin exhibited a dose-dependent activity.
⁎ Corresponding author. E-mail address: [email protected] (V.V. Golovchenko). Peer review under responsibility of Far Eastern Federal University.
http://dx.doi.org/10.1016/j.als.2016.12.034 2078-1520/© 2017 Far Eastern Federal University. Hosting by Elsevier B.V. All rights reserved.