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Studies of the Avian Infectious Bronchitis Virus (Wachtel Strain) in the Cynomolgus Monkey
1448
RESEARCH NOTES
STUDIES OF T H E AVIAN I N F E C T I O U S B R O N C H I T I S VIRUS (WACHTEL STRAIN) I N T H E CYNOMOLGUS M O N K E Y R E G I N A L D L. R E A G A N , F R A N C E S Y A N C E Y AND A. L. B R U E C K N E R
Virus Laboratory, Live Stock Sanitary Service, University of Maryland, College Park, Maryland, (Received for publication August 10, 1955)
were fed on a diet of bananas, oranges, bread, evaporated milk, greens and vitamins. T h e y were carefully checked in the morning and afternoon with daily temperature and were held for a 14 day observation period. Results are shown in Table 1. TABLE 1.'—Response of cynomolgus monkey to infectious bronchitis virus after intranasal instillation and response of cynomolgus monkey to normal allantoic chick embryofluidafter intranasal instillation Monkey No.
Showing respiratory symptoms
Temperature
1 2 3 4 5 6 Control 7 8
None None None None None None None None None
Normal Normal Normal Normal Normal Normal Normal Normal Normal
T h e infectious bronchitis virus used in this study was obtained from Dr. F . R. Beaudette of the New Jersey Agricultural Experiment Station, who isolated it from infected birds which were proved to have infectious bronchitis. T h e strain was the 28th passage of the Wachtel 1BV. I t was subjected to one additional passage a t this laboratory. One-hundred t w e n t y hours after injection of the 28th passage into eggs, the embryos died, and allantoic fluid was collected from each and pooled. Normal eggs injected with normal egg allantoic -amniotic fluid was done likewise. Sterility tests from test and control fluid indicated t h a t the material was bacteria free.
Six of the exposed monkeys showed no sign of infectious bronchitis during a 14d a y observation period and the two controls appeared normal. From this observation monkeys are n o t susceptible to this infectious bronchitis strain (Wachtel).
Eight young monkeys (Cynomolgus, weight 2 lbs.) which h a d been tuberculosis tested and were in excellent health were held in isolation for 7 days. T h e y remained completely normal throughout this period. One ml. of the virus-bearing allantoic-amniotic yolk fluid (prepared as same technique as above) was instilled nasally into two monkeys and monkeys
Bushner, L. D., and C. A. Brandly, 1933. Laryngotracheitis in chicks. Poultry Sci. 21: SS-60. Delaha, E. C, R. L. Reagan, S. R. Cook and A. L. Brueckner, 1954. Transmission of the avian infectious bronchitis virus to the cynomolgus monkey. Cornell Vet. XLIV: 433-436. Schalk, A. F., and M. C. Hawn, 1931. An apparently new respiratory disease of baby chicks. J. Amer. Vet. Assoc. 78:413^20.
SUMMARY
REFERENCES
Downloaded from http://ps.oxfordjournals.org/ at University of Otago Science Library on April 22, 2015
Schalk and Hawn (1931) first described avian infectious bronchitis, and Bushnell and Brandly (1933) concluded t h a t a filterable agent was its cause. Since the disease has been definitely identified only in chickens and since it is believed to be specific for the chicken, and when Delaha et al. (1954) were able to produce the disease in monkeys, we thought due to the importance of their findings, t h a t trials for confirmation should be tried using the same strain as used previously for infectious bronchitis virus.
RESEARCH NOTES
STUDIES OF T H E AVIAN I N F E C T I O U S B R O N C H I T I S VIRUS (WACHTEL STRAIN) I N T H E CYNOMOLGUS M O N K E Y R E G I N A L D L. R E A G A N , F R A N C E S Y A N C E Y AND A. L. B R U E C K N E R
Virus Laboratory, Live Stock Sanitary Service, University of Maryland, College Park, Maryland, (Received for publication August 10, 1955)
were fed on a diet of bananas, oranges, bread, evaporated milk, greens and vitamins. T h e y were carefully checked in the morning and afternoon with daily temperature and were held for a 14 day observation period. Results are shown in Table 1. TABLE 1.'—Response of cynomolgus monkey to infectious bronchitis virus after intranasal instillation and response of cynomolgus monkey to normal allantoic chick embryofluidafter intranasal instillation Monkey No.
Showing respiratory symptoms
Temperature
1 2 3 4 5 6 Control 7 8
None None None None None None None None None
Normal Normal Normal Normal Normal Normal Normal Normal Normal
T h e infectious bronchitis virus used in this study was obtained from Dr. F . R. Beaudette of the New Jersey Agricultural Experiment Station, who isolated it from infected birds which were proved to have infectious bronchitis. T h e strain was the 28th passage of the Wachtel 1BV. I t was subjected to one additional passage a t this laboratory. One-hundred t w e n t y hours after injection of the 28th passage into eggs, the embryos died, and allantoic fluid was collected from each and pooled. Normal eggs injected with normal egg allantoic -amniotic fluid was done likewise. Sterility tests from test and control fluid indicated t h a t the material was bacteria free.
Six of the exposed monkeys showed no sign of infectious bronchitis during a 14d a y observation period and the two controls appeared normal. From this observation monkeys are n o t susceptible to this infectious bronchitis strain (Wachtel).
Eight young monkeys (Cynomolgus, weight 2 lbs.) which h a d been tuberculosis tested and were in excellent health were held in isolation for 7 days. T h e y remained completely normal throughout this period. One ml. of the virus-bearing allantoic-amniotic yolk fluid (prepared as same technique as above) was instilled nasally into two monkeys and monkeys
Bushner, L. D., and C. A. Brandly, 1933. Laryngotracheitis in chicks. Poultry Sci. 21: SS-60. Delaha, E. C, R. L. Reagan, S. R. Cook and A. L. Brueckner, 1954. Transmission of the avian infectious bronchitis virus to the cynomolgus monkey. Cornell Vet. XLIV: 433-436. Schalk, A. F., and M. C. Hawn, 1931. An apparently new respiratory disease of baby chicks. J. Amer. Vet. Assoc. 78:413^20.
SUMMARY
REFERENCES
Downloaded from http://ps.oxfordjournals.org/ at University of Otago Science Library on April 22, 2015
Schalk and Hawn (1931) first described avian infectious bronchitis, and Bushnell and Brandly (1933) concluded t h a t a filterable agent was its cause. Since the disease has been definitely identified only in chickens and since it is believed to be specific for the chicken, and when Delaha et al. (1954) were able to produce the disease in monkeys, we thought due to the importance of their findings, t h a t trials for confirmation should be tried using the same strain as used previously for infectious bronchitis virus.