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Study of interaction between human serum albumin and Norfloxacin in the presence of counter ions using synchronous fluorescence spectroscopy
S170
Abstracts
[email protected] (K. Aberumand), [email protected] (M. Zakerzade) Introduction: Acid phosphatases (ACP) (EC 3.1.3.4) belong to the hydrolase class of enzymes and occur as several isoenzymes with a common enzymatic function (the hydrolytic breakdown of phosphate monoesters). They all show optimal enzyme activity below a pH of 7.0. They possess some tissue specificity (greatest concentrations occur in prostate, liver, spleen, and bone). The major forms are coded for by different genes, and also possess different molecular weights and structures. Methods: The human placenta was hemogenised in saline solution and then buthanol and acetone was used for isolation and purification of ACP. Then with utilization of sulphate ammonium 30% and 70%, the ACP was rest in supernatant and then bottom of the solution respectively. Sulphate ammonium was isolated with dialysis and concentrated enzyme with utilization of Gel-filtration chromatography and G-100 sephadex was divided to different fractions. After collection the different fractions, estimated the protein concentration in 280 nm and enzyme activity. Results: The result of this investigation was marked that ACP is present in human placenta and their different fractions. Conclusion: The prescence of ACP in human placenta can utilizes in future scientific progect and probably application for detection of placental disease. Keywords: Enzyme, Acid phosphatase (ACP), Enzyme activity, Placenta doi:10.1016/j.clinbiochem.2011.08.431
Poster – [A-10-984-2] Disorders of glucose challenge test (GCT) in pregnant women referred to laboratory Khatam-o-allanbia of Arak city in 2009 Alimohammadi Mohammad, Rostami Morad Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran E-mail addresses: [email protected] (A. Mohammad), [email protected] (R. Morad) Introduction: Incidence of glucose challenge test (GCT) disorders in verified diabetics pregnant women, to take into consideration of GCT ≥ 140 and GCT ≥ 130 mg/dl, 80% and 90% were shown, respectively. Incidence od gestational diabetes in IRAN, variable between 1.3-8.9%. Materials and Methods: In this cross-sectional study, from each referred pregnant woman during 24–28 weeks of gestation to laboratory Khatam-o-allanbia of Arak city in 2009, 5 ml of the venous blood was drawn and tests were done with Mindary BS-300 autoanalyser and kits of Pars azmun company. Results: The average age of freefred pregnant women are 27.37 ± 10.24 (16–39) years. From 417 pregnant women, 14 (3.36%) individuals have abnormal fasting plasma glucose (FPG). To take into consideration of GCT ≥ 140 and GCT ≥ 130 mg/dl, 60 (14.39%) and 109 (26.14%) individuals were have abnormal GCT. Average FPG and GCT were, 83.86 (73–243) and 118.32 (140–351) mg/dl, respectively. In 9 (64.29%) individual with abnormal FPG, the GCT test were too abnormal. Conclusion: This study, showed that GCT disorders in women with abnormal FPG, were higher and in cases with normal FPG too, always to have in mind the probability of abnormal GCT. Keywords: Glucose challenge test (GCT), Fasting plasma glucose (FPG) doi:10.1016/j.clinbiochem.2011.08.432
Poster – [A-10-989-1] Study of interaction between human serum albumin and Norfloxacin in the presence of counter ions using synchronous fluorescence spectroscopy Behnaz Bakaeiana, Mohammad Reza Saberib, Jamshid Khan Chamania a Department of Biology, Faculty of Sciences, Islamic Azad University-Mashhad Branch, Mashhad, Iran b Medical Chemistry Department, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran E-mail address: [email protected] (B. Bakaeian) Introduction: Protein–drug interaction plays an important role in pharmacokinetics and pharmacodynamics of the drugs. This interaction can take place with a variety of agents in blood, including proteins such as human serum albumin (HSA). HSA has been used as a model protein for many years. Human serum albumin, the most abundant protein in plasma, has the capability to bind a wide range of endogenous and exogenous compounds. Norfloxacin,1-ethyl-6-fluoro-1,4-dihydro-4oxo-7-(1-piperazinyl)-3-quinoline carboxylic acid is a synthetic broad spectrum antibacterial drug, which is mainly used in the treatment of urinary tract infections. Synchronous fluorescence technique gives information about the molecular environment in a vicinity of the chromophore molecule. When D-value (Δλ) between excitation and emission wavelength is stabilized at 15 or 60 nm, the synchronous fluorescence gives the typical information of tyrosine and tryptophan residues, respectively. The binding of Norfloxacin to Human serum albumin in presence of various kind of ions (Al - Pb - Cu - Mg ) has been investigated using Synchronous fluorescence technique. Conclusion: Our results show that, with increasing concentration of counter ions and a fixed concentration of HAS with NRF, maximum emission wavelength of HAS has afaint red shift upon binding of different ions. In presence of Al and Cu maximum emission of HAS and NRF has an obvious red shift when Δλ is 60 nm. The red shift expressed that the conformational changes of Trp and Tyr microenvironment. This study can predict the effect of counter ions on the function of Norfloxacin in drug therapy. Keywords: Human serum albumin, Norfloxacin, Counter ions, Synchronous fluorescence spectroscopy
doi:10.1016/j.clinbiochem.2011.08.433
Poster — [A-10-991-1] Nandrolone decanoate's effects on biochemical blood parameters in rat Mohammad Reza Ehsan, Seyed Majdodin Vahidi Tourchi, Ali Mahdavi No 139, Shahid Mozdorani alley, Sepah Street, Iran E-mail addresses: [email protected] (M.R. Ehsan), [email protected] (S.M. Vahidi Tourchi) Introduction: Anemia is one of the most common side effects of surgeries or disorders following bleeding. It may not be easily treated by prescribed supplements or it is even incurable. A wide range of medications, hormones and vitamins motivate blood generation and can promote the situation. Nandrolone decanoate is one of medications that could cure Aplastic anemia. Therefore, following animal's patterns in order to find out the side effects and the function seems necessary. Material and methods: To carry out this study, 20 rats were divided to two groups and fed with the same diet. The test group had 2.5 mg/kg Nandrolone decanoate IM weekly for 4 weeks while the control group was fed with regular diet. After the period, the blood samples were taken and analyzed by biochemical and hematological
Abstracts
[email protected] (K. Aberumand), [email protected] (M. Zakerzade) Introduction: Acid phosphatases (ACP) (EC 3.1.3.4) belong to the hydrolase class of enzymes and occur as several isoenzymes with a common enzymatic function (the hydrolytic breakdown of phosphate monoesters). They all show optimal enzyme activity below a pH of 7.0. They possess some tissue specificity (greatest concentrations occur in prostate, liver, spleen, and bone). The major forms are coded for by different genes, and also possess different molecular weights and structures. Methods: The human placenta was hemogenised in saline solution and then buthanol and acetone was used for isolation and purification of ACP. Then with utilization of sulphate ammonium 30% and 70%, the ACP was rest in supernatant and then bottom of the solution respectively. Sulphate ammonium was isolated with dialysis and concentrated enzyme with utilization of Gel-filtration chromatography and G-100 sephadex was divided to different fractions. After collection the different fractions, estimated the protein concentration in 280 nm and enzyme activity. Results: The result of this investigation was marked that ACP is present in human placenta and their different fractions. Conclusion: The prescence of ACP in human placenta can utilizes in future scientific progect and probably application for detection of placental disease. Keywords: Enzyme, Acid phosphatase (ACP), Enzyme activity, Placenta doi:10.1016/j.clinbiochem.2011.08.431
Poster – [A-10-984-2] Disorders of glucose challenge test (GCT) in pregnant women referred to laboratory Khatam-o-allanbia of Arak city in 2009 Alimohammadi Mohammad, Rostami Morad Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran E-mail addresses: [email protected] (A. Mohammad), [email protected] (R. Morad) Introduction: Incidence of glucose challenge test (GCT) disorders in verified diabetics pregnant women, to take into consideration of GCT ≥ 140 and GCT ≥ 130 mg/dl, 80% and 90% were shown, respectively. Incidence od gestational diabetes in IRAN, variable between 1.3-8.9%. Materials and Methods: In this cross-sectional study, from each referred pregnant woman during 24–28 weeks of gestation to laboratory Khatam-o-allanbia of Arak city in 2009, 5 ml of the venous blood was drawn and tests were done with Mindary BS-300 autoanalyser and kits of Pars azmun company. Results: The average age of freefred pregnant women are 27.37 ± 10.24 (16–39) years. From 417 pregnant women, 14 (3.36%) individuals have abnormal fasting plasma glucose (FPG). To take into consideration of GCT ≥ 140 and GCT ≥ 130 mg/dl, 60 (14.39%) and 109 (26.14%) individuals were have abnormal GCT. Average FPG and GCT were, 83.86 (73–243) and 118.32 (140–351) mg/dl, respectively. In 9 (64.29%) individual with abnormal FPG, the GCT test were too abnormal. Conclusion: This study, showed that GCT disorders in women with abnormal FPG, were higher and in cases with normal FPG too, always to have in mind the probability of abnormal GCT. Keywords: Glucose challenge test (GCT), Fasting plasma glucose (FPG) doi:10.1016/j.clinbiochem.2011.08.432
Poster – [A-10-989-1] Study of interaction between human serum albumin and Norfloxacin in the presence of counter ions using synchronous fluorescence spectroscopy Behnaz Bakaeiana, Mohammad Reza Saberib, Jamshid Khan Chamania a Department of Biology, Faculty of Sciences, Islamic Azad University-Mashhad Branch, Mashhad, Iran b Medical Chemistry Department, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran E-mail address: [email protected] (B. Bakaeian) Introduction: Protein–drug interaction plays an important role in pharmacokinetics and pharmacodynamics of the drugs. This interaction can take place with a variety of agents in blood, including proteins such as human serum albumin (HSA). HSA has been used as a model protein for many years. Human serum albumin, the most abundant protein in plasma, has the capability to bind a wide range of endogenous and exogenous compounds. Norfloxacin,1-ethyl-6-fluoro-1,4-dihydro-4oxo-7-(1-piperazinyl)-3-quinoline carboxylic acid is a synthetic broad spectrum antibacterial drug, which is mainly used in the treatment of urinary tract infections. Synchronous fluorescence technique gives information about the molecular environment in a vicinity of the chromophore molecule. When D-value (Δλ) between excitation and emission wavelength is stabilized at 15 or 60 nm, the synchronous fluorescence gives the typical information of tyrosine and tryptophan residues, respectively. The binding of Norfloxacin to Human serum albumin in presence of various kind of ions (Al - Pb - Cu - Mg ) has been investigated using Synchronous fluorescence technique. Conclusion: Our results show that, with increasing concentration of counter ions and a fixed concentration of HAS with NRF, maximum emission wavelength of HAS has afaint red shift upon binding of different ions. In presence of Al and Cu maximum emission of HAS and NRF has an obvious red shift when Δλ is 60 nm. The red shift expressed that the conformational changes of Trp and Tyr microenvironment. This study can predict the effect of counter ions on the function of Norfloxacin in drug therapy. Keywords: Human serum albumin, Norfloxacin, Counter ions, Synchronous fluorescence spectroscopy
doi:10.1016/j.clinbiochem.2011.08.433
Poster — [A-10-991-1] Nandrolone decanoate's effects on biochemical blood parameters in rat Mohammad Reza Ehsan, Seyed Majdodin Vahidi Tourchi, Ali Mahdavi No 139, Shahid Mozdorani alley, Sepah Street, Iran E-mail addresses: [email protected] (M.R. Ehsan), [email protected] (S.M. Vahidi Tourchi) Introduction: Anemia is one of the most common side effects of surgeries or disorders following bleeding. It may not be easily treated by prescribed supplements or it is even incurable. A wide range of medications, hormones and vitamins motivate blood generation and can promote the situation. Nandrolone decanoate is one of medications that could cure Aplastic anemia. Therefore, following animal's patterns in order to find out the side effects and the function seems necessary. Material and methods: To carry out this study, 20 rats were divided to two groups and fed with the same diet. The test group had 2.5 mg/kg Nandrolone decanoate IM weekly for 4 weeks while the control group was fed with regular diet. After the period, the blood samples were taken and analyzed by biochemical and hematological