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Study of the cutaneous microcirculation in tetralogy of fallot
110 008 REGULATION OF TRANSGLUTAMINASE TYPE 1 IN CULTURED NORMAL HUMAN KERATINOCYTES Y AOYAMA* M.SEISHIMA* S.MORI* Y.OKANO** Y.NOZAWA** M.SEISHIMA*** and A.NOMA ***, Departments of *Dermatology, **Biochemistry and ***Laboratory medicine, Gifu University School of Medicine, 40 Tukasa-machi Gifu city Gifu Japan Epidermal differentiation is characterized by a series of coordinated morphological and biochemical changes resulting in a highly specialized, highly organized, stratified squamous epithelia. Cultured human keratinocytes express a transglutaminase Type 1 which appears in epidermis only during later stages of normal keratinocyte differentiation. Northern blot analysis using specific cDNA probe for transglutaminase Type 1 showed that exposure of normal human keratinocytes to high calcium (1.2mM) medium caused a marked increase in mRNA levels of this enzyme. This change was observed as early as 12h after the calcium concentration in medium was increased from 0.05mM to 1.2mM. Maximal induction of transglutaminase Type 1 mRNA occurred in 18h. Phorbol ester treatment of cultured normal human keratinocytes in high-calcium medium induced increase in mRNA levels of transglutaminase Type 1. These results suggest that calcium is a key component for regulation of transglutaminasc Type 1 mRNA through the action of protein kinase C.
009 EFFECT OF MINOXIDIL-SULFATE ON CULTURED WCs) AND ROOT SHEATH FIHROHLASTS(RSFsl.
HUMAS HAIR PAPII.L1 CELLS
4.ARAI! C.SEIDEl: K.KATSUOKA AND SNISHIYAYA 'Department of Dermatology, Kitasato University School of Neditine. I 15 1. Kitasato. Sagamihara.Japan and *Department of Dermatology. Friedrich-Alexander University. Hartmannstr.14. Erlangen. Germany. Ruhl et al. demonstrated that YinoxidiI~sulfate(YNX-So can be a mandatory metabolite affecting hair growth. To confirm the effects of MNX-S on hair grouth,we used human papilla celIs(PCs~ and root sheath fibroblasts(RSFs) and exposed various concentrations of MNX-S in vitro The result of immunohistochemical staining of S~Rromo~Z~deoxy-uridine revealed over 70 )&ml of MNX-S was toxic for both human PCs and RSFs. 15.day growth curves were obtained under various concentrations of MNX-S from 5 UP to 70 &ml.As the results. 5 IO pa/ml proved to be the idial concentration for the growth in vitro. Quite low concentration of MNX-S can affect on growth of the cultured PCs. Our results SURReSt that MNX S stimulates not only hair follicle keratinocytes but also dermal PCs in r-ivo.
012 STUDY OF THE CUTANEOUS TETRALOGY
CHUNG-"SING
MICROCIRCULATION
IN
OF FALLOT CHANG,
HSIN-SU
YU
Department of Dermatology, Kaohslung Medical College, No.100, Shih-Chuan 1st Road, Ksohsiung, Taiwan, R.O.C. Cutaneous microcirculationwas studied in 15 cases of Tetralogy of F&lot for the effect of long-term hypoxia. There were 7 males and 8 females, ranging in age fran 2 to 16 years and 20 healthy children with age match served as Controls. The capillary microscope-television-computersystem was used for nailfold capillary morphology analysis and velocity measurement. We found that nailfold capillaries became dilated, torturous and branching with increase of total rngth and vascular area. The degree of dilatation and vascularlty was closely related to the hemoglobin concentration. Capillary blood cell velocity declined with the increase of hematocnt, significant decrease was noted during Hb>19 g/d1 or Hct>60 % (0.08 ? 0.05 vs 0.60 + 0.32 rrrn/sec. pc 1.05). Laser Doppler flowretry reveaied significant increase of perfusion in clubbng fingers (82.2 i 19.1 vs 61.9 t8.0 p.u., ~~0.05). Vasorrotionwave was reserved with 6-10 cycle/run except u? extrernelydilated vessels.
009 EFFECT OF MINOXIDIL-SULFATE ON CULTURED WCs) AND ROOT SHEATH FIHROHLASTS(RSFsl.
HUMAS HAIR PAPII.L1 CELLS
4.ARAI! C.SEIDEl: K.KATSUOKA AND SNISHIYAYA 'Department of Dermatology, Kitasato University School of Neditine. I 15 1. Kitasato. Sagamihara.Japan and *Department of Dermatology. Friedrich-Alexander University. Hartmannstr.14. Erlangen. Germany. Ruhl et al. demonstrated that YinoxidiI~sulfate(YNX-So can be a mandatory metabolite affecting hair growth. To confirm the effects of MNX-S on hair grouth,we used human papilla celIs(PCs~ and root sheath fibroblasts(RSFs) and exposed various concentrations of MNX-S in vitro The result of immunohistochemical staining of S~Rromo~Z~deoxy-uridine revealed over 70 )&ml of MNX-S was toxic for both human PCs and RSFs. 15.day growth curves were obtained under various concentrations of MNX-S from 5 UP to 70 &ml.As the results. 5 IO pa/ml proved to be the idial concentration for the growth in vitro. Quite low concentration of MNX-S can affect on growth of the cultured PCs. Our results SURReSt that MNX S stimulates not only hair follicle keratinocytes but also dermal PCs in r-ivo.
012 STUDY OF THE CUTANEOUS TETRALOGY
CHUNG-"SING
MICROCIRCULATION
IN
OF FALLOT CHANG,
HSIN-SU
YU
Department of Dermatology, Kaohslung Medical College, No.100, Shih-Chuan 1st Road, Ksohsiung, Taiwan, R.O.C. Cutaneous microcirculationwas studied in 15 cases of Tetralogy of F&lot for the effect of long-term hypoxia. There were 7 males and 8 females, ranging in age fran 2 to 16 years and 20 healthy children with age match served as Controls. The capillary microscope-television-computersystem was used for nailfold capillary morphology analysis and velocity measurement. We found that nailfold capillaries became dilated, torturous and branching with increase of total rngth and vascular area. The degree of dilatation and vascularlty was closely related to the hemoglobin concentration. Capillary blood cell velocity declined with the increase of hematocnt, significant decrease was noted during Hb>19 g/d1 or Hct>60 % (0.08 ? 0.05 vs 0.60 + 0.32 rrrn/sec. pc 1.05). Laser Doppler flowretry reveaied significant increase of perfusion in clubbng fingers (82.2 i 19.1 vs 61.9 t8.0 p.u., ~~0.05). Vasorrotionwave was reserved with 6-10 cycle/run except u? extrernelydilated vessels.