- Email: [email protected]
Study on bacterial physiological response towards different classes of pharmaceuticals
Journal of Biotechnology 239S (2016) 1–3
Contents lists available at ScienceDirect
Journal of Biotechnology journal homepage: www.elsevier.com/locate/jbiotec
Abstracts of supplement issue “Euro Biotech 2016 Supplement”
A novel method of teaching information technology applied in health monitoring Gabriela Gladiola Andruseac 1,∗ , Sanda Victorinne Paturca 2 , Cosmin Karl Banica 2 , Ilona Madalina Costea 3 , Cristian Rotariu 1 1
Faculty of Medical Bioengineering, “Grigore T. Popa” University of Medicine and Pharmacy, Iasi, Romania 2 Faculty of Electrical Engineering, University “Politehnica” of Bucharest, Romania 3 Faculty of Transports, University “Politehnica” of Bucharest, Romania E-mail address: [email protected] (G.G. Andruseac). It’s a present reality that Information Technology and Communication (ITC), combined with health monitoring wearable devices, gain more and more popularity. The authors of this paper took that as an opportunity to effectively teach university students the key elements regarding the application of IT in health monitoring. The method proposed in this paper consists in involving students in project-oriented learning and working. It was chosen an attractive wearable device as classroom equipment – a chest belt having integrated ECG monitoring circuits, accelerometers, temperature sensor etc. The project has moderate to high complexity, mainly due to its inter-disciplinary character. Project team members are organized in groups, each one having assigned a specific task to complete and to present to the other groups. The main covered topics are: • Sensors and signal conditioning, communication, all integrated in the wearable equipment; • Development of PC application for real-time data visualization, processing and storing, for a specified application scenario (e.g. subjects are members of a sport team); • Development of data-center: database server and management, database structured according to the designated scenario; • Development of client application for user-friendly query and visualization; • Working with powerful software tools: Matlab, LabVIEW.
By being involved in the big project with their part of work, students are motivated to do their best for the project success.
0168-1656/
Acknowledgement: The work has been carried out within the program Joint Applied Research Projects, funded by the Romanian National Authority for Scientific Research, project PN-II-PT-PCCA2013-4-0761, no. 21/2014 (SIACT). http://dx.doi.org/10.1016/j.jbiotec.2016.09.019 Study on bacterial physiological response towards different classes of pharmaceuticals Madars Davids 1,∗ , Vadims Bartkevics 2 , Olga Muter 1 , Turs Selga 3 1 Institute of Microbiology & Biotechnology University of Latvia, Riga, Latvia 2 Faculty of Chemistry, University of Latvia, Riga, Latvia 3 Faculty of Biology, University of Latvia, Riga, Latvia
E-mail address: [email protected] (M. Davids). The occurrence of pharmaceuticals entering the water cycle attracts an increasing attention of water resource institutions and society. Toxicity of these pollutants could considerably inhibit biological processes taking place in the wastewater treatment. This study was aimed at comparing the toxicity of 29 pharmaceuticals for Pseudomonas fluorescens and Micrococcus luteus, as well as for five bacterial consortia. Bacterial consortia were previously isolated either from soils or wastewaters contaminated with different organics e.g., hydrocarbons, detergents, cellulose. Bacteria response to the presence of pharmaceuticals (1 mg/L) was evaluated by growth inhibition in liquid broth, as well as colony forming ability, swarming motility on agarized medium and confocal laser scanning microscopy for possible morphological changes determination. The most pronounced inhibition in the liquid medium for P. fluorescens was detected by ciprofloxacin, whereas for M. luteus – ciprofloxacin, azithromycin, clarithromycin, erythromycin A and estriol, respectively. Swarming motility of P. fluorescens on a soft (0.3%) agar was reduced in the presence of azithromycin and ciprofloxacin up to 45.2% and 81.7%, respectively, as compared to control. Binary mixtures, i.e., combination of ciprofloxacin with atorvastatin, estrone, valsartan and xylazine strengthened an inhibition of swarming
2
Abstracts / Journal of Biotechnology 239S (2016) 1–3
motility as compared to single ciprofloxacin. However these chemicals did not show the inhibition effect, when added separately. The results indicate that ciprofloxacin possesses the strongest antibacterial effect among 29 compounds tested. Acknowledgement: Research was financially supported by the Project No. NFI/R/2014/010 “Establishing of the scientific capacity for the management of pharmaceutical products residues in the environment of Latvia and Norway”. http://dx.doi.org/10.1016/j.jbiotec.2016.09.020 The use of probiotics in cattle feeding: The health status of calves Monica Parvu 1,∗ , Violeta Elena Simion 1 , Elena Mitranescu 2 , Laurentiu Tudor 2 1
Spiru Haret University, Faculty of Veterinary Medicine, Bucharest, Romania 2 University of Agricultural Sciences and Veterinary Medicine, Faculty of Veterinary Medicine, Bucharest, Romania E-mail address: [email protected] (M. Parvu). The experiment was conducted in a private farm with semiintensive system of rearing cattle. The thirty-two Holstein cows were divided in two groups: control (C1) and experimental (E1). Only the cows in the experimental group received probiotics, since the last trimester of gestation until the second month of lactation. The polyculture was obtained from Lactobacillus species (L. acidophilus + L. plantarum + L. casei) and Enterococcus faecium. The strains were not genetically modified. After birth, the calves obtained from the control group did not receive probiotics (C2); the calves from the experimental group consumed milk from cows fed with probiotics (E2). Diarrhoea has been reported at C2 group, causing 4.5% mortality and 5.1% loss in body weight. The calves received 50 ml of probiotics per day; the diarrhoea stopped after three days of treatment. At E2 group, the calves did not have diarrhoea, as an effect of the use of probiotics in nutrition of mother-cows. http://dx.doi.org/10.1016/j.jbiotec.2016.09.021 Detection of heat loss in a dryer and pellet stove installation using infrared thermography technique F.J. Moral ∗ , Raúl Kassir Alkarany Department of Graphic Expression Engineering, School of Industrial Engineering, University of Extremadura, Spain E-mail address: fwa [email protected] (F.J. Moral). A nondestructive thermal study has been developed in the present study using infrared thermography technique for the detection of heat loss from a drying installation. A laboratory screw dryer was used as part of the installation. A biomass stove was used to provide hot gases as a drying medium for the drying process of grape pomace. A thermographic inspection has been realized during the experiments as an application, to evaluate the thermal patterns of the installation and its constructive elements such as, pipes and conduits of hot gases of combustion. The temperatures of different parts of the dryer have been measured at various points along the dryer using an infrared camera. The thermal images obtained have been analyzed using a PC software. The results shown that the measured temperatures were decreased
along the dryer because of heat transfer to the grape pomance, the dryer drum and the exterior (as a heat loss). The work has shown the utility of this technique in detecting various non-visible leakages in energetic and thermal installations Keywords: Biomass; Drying; Heat transfer; Thermography http://dx.doi.org/10.1016/j.jbiotec.2016.09.022 Evaluation of nitrates and nitrites content in some vegetables Alina-Madalina Lato 1 , Oana Suciu 1 , Cristina Petrescu 1 , Brigitha Vlaicu 1 , Matilda Radulescu 1 , Adina Berbecea 2 , Isidora Radulov 2 , Iaroslav Lato 2,∗ 1
Victor Babes¸ University of Medicine and Pharmacy, Timis¸oara, Romania 2 University of Agricultural Sciences and Veterinary Medicine of Banat, King Michael I of Romania, Timis¸oara, Romania E-mail address: [email protected] (I. Lato). Vegetables are a vital part of the human diet, being a source of minerals, vitamins, fiber. They contain nitrates and nitrites, which affect human health. By reduction, they form the nitrites who determine methaemoglobinaemia and cancer. Acceptable daily intake for 60 kg body weight is 220 mg/person-nitrates and 8 mg/personnitrites. We selected the vegetables (turnip, cucumber, spinach, lettuce, celery stem and leafs), because this are the most consumed ones, information obtained on a questioner applied to 100 persons. In the summer, the lowest amount of nitrates was determinate in case of cucumbers 21.264 mg/kg and the highest one in case of spinach 327.82 mg/kg. In the winter period the lowest accumulation of nitrates was determined for lettuce 48.730 mg/kg but the celery stems accumulate the maximum amount of 398.700 mg/kg. In case of nitrites, we determine a minimum content 98.7 mg/kg for lettuce in the summer and a maximum content 345.45 mg/kg for spinach. During the winter we obtained the lowest amount for lettuce 115.15 mg/kg and the highest amount for spinach 658 mg/kg. In all the studied vegetables nitrite content are not exceeding the values presented in the literature, being higher in winter than in summer as in the case of nitrates. http://dx.doi.org/10.1016/j.jbiotec.2016.09.023 Study on diversity of strongyle infective larvae populations in naturally infected horses, Romania Sabrina Maria Anutescu, Marius Catalin Buzatu ∗ , Alexandra Gruianu, Andreea Cristina Paltin, Ioan Liviu Mitrea, Mariana Ionita Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine of Bucharest, Romania E-mail address: [email protected] (M.C. Buzatu). Knowledge on species composition of equid strongylid communities is essential for biological and epidemiological studies. Often, mixed infections occur but the similar morphology of strongyle eggs does not allow species differentiation. Therefore, the present study aimed to examine the structure of strongyle populations in horses on the basis of the morphology of the third-larval (L3)
Contents lists available at ScienceDirect
Journal of Biotechnology journal homepage: www.elsevier.com/locate/jbiotec
Abstracts of supplement issue “Euro Biotech 2016 Supplement”
A novel method of teaching information technology applied in health monitoring Gabriela Gladiola Andruseac 1,∗ , Sanda Victorinne Paturca 2 , Cosmin Karl Banica 2 , Ilona Madalina Costea 3 , Cristian Rotariu 1 1
Faculty of Medical Bioengineering, “Grigore T. Popa” University of Medicine and Pharmacy, Iasi, Romania 2 Faculty of Electrical Engineering, University “Politehnica” of Bucharest, Romania 3 Faculty of Transports, University “Politehnica” of Bucharest, Romania E-mail address: [email protected] (G.G. Andruseac). It’s a present reality that Information Technology and Communication (ITC), combined with health monitoring wearable devices, gain more and more popularity. The authors of this paper took that as an opportunity to effectively teach university students the key elements regarding the application of IT in health monitoring. The method proposed in this paper consists in involving students in project-oriented learning and working. It was chosen an attractive wearable device as classroom equipment – a chest belt having integrated ECG monitoring circuits, accelerometers, temperature sensor etc. The project has moderate to high complexity, mainly due to its inter-disciplinary character. Project team members are organized in groups, each one having assigned a specific task to complete and to present to the other groups. The main covered topics are: • Sensors and signal conditioning, communication, all integrated in the wearable equipment; • Development of PC application for real-time data visualization, processing and storing, for a specified application scenario (e.g. subjects are members of a sport team); • Development of data-center: database server and management, database structured according to the designated scenario; • Development of client application for user-friendly query and visualization; • Working with powerful software tools: Matlab, LabVIEW.
By being involved in the big project with their part of work, students are motivated to do their best for the project success.
0168-1656/
Acknowledgement: The work has been carried out within the program Joint Applied Research Projects, funded by the Romanian National Authority for Scientific Research, project PN-II-PT-PCCA2013-4-0761, no. 21/2014 (SIACT). http://dx.doi.org/10.1016/j.jbiotec.2016.09.019 Study on bacterial physiological response towards different classes of pharmaceuticals Madars Davids 1,∗ , Vadims Bartkevics 2 , Olga Muter 1 , Turs Selga 3 1 Institute of Microbiology & Biotechnology University of Latvia, Riga, Latvia 2 Faculty of Chemistry, University of Latvia, Riga, Latvia 3 Faculty of Biology, University of Latvia, Riga, Latvia
E-mail address: [email protected] (M. Davids). The occurrence of pharmaceuticals entering the water cycle attracts an increasing attention of water resource institutions and society. Toxicity of these pollutants could considerably inhibit biological processes taking place in the wastewater treatment. This study was aimed at comparing the toxicity of 29 pharmaceuticals for Pseudomonas fluorescens and Micrococcus luteus, as well as for five bacterial consortia. Bacterial consortia were previously isolated either from soils or wastewaters contaminated with different organics e.g., hydrocarbons, detergents, cellulose. Bacteria response to the presence of pharmaceuticals (1 mg/L) was evaluated by growth inhibition in liquid broth, as well as colony forming ability, swarming motility on agarized medium and confocal laser scanning microscopy for possible morphological changes determination. The most pronounced inhibition in the liquid medium for P. fluorescens was detected by ciprofloxacin, whereas for M. luteus – ciprofloxacin, azithromycin, clarithromycin, erythromycin A and estriol, respectively. Swarming motility of P. fluorescens on a soft (0.3%) agar was reduced in the presence of azithromycin and ciprofloxacin up to 45.2% and 81.7%, respectively, as compared to control. Binary mixtures, i.e., combination of ciprofloxacin with atorvastatin, estrone, valsartan and xylazine strengthened an inhibition of swarming
2
Abstracts / Journal of Biotechnology 239S (2016) 1–3
motility as compared to single ciprofloxacin. However these chemicals did not show the inhibition effect, when added separately. The results indicate that ciprofloxacin possesses the strongest antibacterial effect among 29 compounds tested. Acknowledgement: Research was financially supported by the Project No. NFI/R/2014/010 “Establishing of the scientific capacity for the management of pharmaceutical products residues in the environment of Latvia and Norway”. http://dx.doi.org/10.1016/j.jbiotec.2016.09.020 The use of probiotics in cattle feeding: The health status of calves Monica Parvu 1,∗ , Violeta Elena Simion 1 , Elena Mitranescu 2 , Laurentiu Tudor 2 1
Spiru Haret University, Faculty of Veterinary Medicine, Bucharest, Romania 2 University of Agricultural Sciences and Veterinary Medicine, Faculty of Veterinary Medicine, Bucharest, Romania E-mail address: [email protected] (M. Parvu). The experiment was conducted in a private farm with semiintensive system of rearing cattle. The thirty-two Holstein cows were divided in two groups: control (C1) and experimental (E1). Only the cows in the experimental group received probiotics, since the last trimester of gestation until the second month of lactation. The polyculture was obtained from Lactobacillus species (L. acidophilus + L. plantarum + L. casei) and Enterococcus faecium. The strains were not genetically modified. After birth, the calves obtained from the control group did not receive probiotics (C2); the calves from the experimental group consumed milk from cows fed with probiotics (E2). Diarrhoea has been reported at C2 group, causing 4.5% mortality and 5.1% loss in body weight. The calves received 50 ml of probiotics per day; the diarrhoea stopped after three days of treatment. At E2 group, the calves did not have diarrhoea, as an effect of the use of probiotics in nutrition of mother-cows. http://dx.doi.org/10.1016/j.jbiotec.2016.09.021 Detection of heat loss in a dryer and pellet stove installation using infrared thermography technique F.J. Moral ∗ , Raúl Kassir Alkarany Department of Graphic Expression Engineering, School of Industrial Engineering, University of Extremadura, Spain E-mail address: fwa [email protected] (F.J. Moral). A nondestructive thermal study has been developed in the present study using infrared thermography technique for the detection of heat loss from a drying installation. A laboratory screw dryer was used as part of the installation. A biomass stove was used to provide hot gases as a drying medium for the drying process of grape pomace. A thermographic inspection has been realized during the experiments as an application, to evaluate the thermal patterns of the installation and its constructive elements such as, pipes and conduits of hot gases of combustion. The temperatures of different parts of the dryer have been measured at various points along the dryer using an infrared camera. The thermal images obtained have been analyzed using a PC software. The results shown that the measured temperatures were decreased
along the dryer because of heat transfer to the grape pomance, the dryer drum and the exterior (as a heat loss). The work has shown the utility of this technique in detecting various non-visible leakages in energetic and thermal installations Keywords: Biomass; Drying; Heat transfer; Thermography http://dx.doi.org/10.1016/j.jbiotec.2016.09.022 Evaluation of nitrates and nitrites content in some vegetables Alina-Madalina Lato 1 , Oana Suciu 1 , Cristina Petrescu 1 , Brigitha Vlaicu 1 , Matilda Radulescu 1 , Adina Berbecea 2 , Isidora Radulov 2 , Iaroslav Lato 2,∗ 1
Victor Babes¸ University of Medicine and Pharmacy, Timis¸oara, Romania 2 University of Agricultural Sciences and Veterinary Medicine of Banat, King Michael I of Romania, Timis¸oara, Romania E-mail address: [email protected] (I. Lato). Vegetables are a vital part of the human diet, being a source of minerals, vitamins, fiber. They contain nitrates and nitrites, which affect human health. By reduction, they form the nitrites who determine methaemoglobinaemia and cancer. Acceptable daily intake for 60 kg body weight is 220 mg/person-nitrates and 8 mg/personnitrites. We selected the vegetables (turnip, cucumber, spinach, lettuce, celery stem and leafs), because this are the most consumed ones, information obtained on a questioner applied to 100 persons. In the summer, the lowest amount of nitrates was determinate in case of cucumbers 21.264 mg/kg and the highest one in case of spinach 327.82 mg/kg. In the winter period the lowest accumulation of nitrates was determined for lettuce 48.730 mg/kg but the celery stems accumulate the maximum amount of 398.700 mg/kg. In case of nitrites, we determine a minimum content 98.7 mg/kg for lettuce in the summer and a maximum content 345.45 mg/kg for spinach. During the winter we obtained the lowest amount for lettuce 115.15 mg/kg and the highest amount for spinach 658 mg/kg. In all the studied vegetables nitrite content are not exceeding the values presented in the literature, being higher in winter than in summer as in the case of nitrates. http://dx.doi.org/10.1016/j.jbiotec.2016.09.023 Study on diversity of strongyle infective larvae populations in naturally infected horses, Romania Sabrina Maria Anutescu, Marius Catalin Buzatu ∗ , Alexandra Gruianu, Andreea Cristina Paltin, Ioan Liviu Mitrea, Mariana Ionita Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine of Bucharest, Romania E-mail address: [email protected] (M.C. Buzatu). Knowledge on species composition of equid strongylid communities is essential for biological and epidemiological studies. Often, mixed infections occur but the similar morphology of strongyle eggs does not allow species differentiation. Therefore, the present study aimed to examine the structure of strongyle populations in horses on the basis of the morphology of the third-larval (L3)