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Study on the inflammatory cytokines produced in jaw cyst walls
025E 6. Study on the Inflammatory Cytokines Produced in Jaw Cyst Walls
Kimura, H., Akanuma, M., Kusumi, A. Department of Dentistry & Oral Surgery, Hirosaki University School of Medicine To investigate the role of inflammatory cytokines in jaw cyst enlargement, the amount of interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-c0 in cyst walls were measured by enzyme-linked immunosorbent assay (ELISA). Furthermore, the localization of both cytokines in cyst wall was investigated by immunohistochemical method. Sixty-five cyst walls, including 14 of dentigerous cyst, 38 of radicular cyst and 13 of postoperative maxillary cyst, and 10 normal gingivae were used as samples. The amount and the positive staining ratio of cytokines were estimated according to the degree of inflammation determined by histological findings in H-E stained section of the same cyst wall. Both cytokines were detected in 41 cyst walls, but the range of values was very wide from 32 to 5034 pg/g wet tissue (IL-6) and from 46 to 1484 pg/g wet tissue (TNF-c0. However, the amount of cytokines reflected the degree of inflammation of cyst wall and there was a significant (P<0.01, n=33) positive correlation between IL-6 and TNFc~in the same samples. According to the classification of jaw cysts, no significant differences were found in the amount of cytokines. Positive stainings for IL-6 and TNF-c~ were observed at the almost same site in cyst wall, that is, at fibrous tissues, epithelial cells, inflammatory cells and blood capillaries. The positive staining ratio of fibrous tissues showed the highest value among those described organs. These findings suggest that both IL-6 and TNF-c~ produced in cyst wall are deeply involved in the bone resorption and the enlargement of jaw cysts.
7. Oral Mucosal Malonedialdehyde (MDA) Levels in Behcet's Patients With and Without Oral Lesions
A. Kaynar, A. 1, Guven, yz, Uslu, E. 3, Yildiz, E. 2, Sarioglu, S. 2, Kose, A. 4, Oner, B. a, Aybar, B. 1, Sarica, R. 4, Azizierli, G.4 Univ~ersity of Istanbul, School of Dentistry, Department of Oral !Surgery, 2 University of Istanbul, School of Dentistry, Dep@tment of Biochemistry, 3University of Istanbul, Cerrahpasa Medical School, Department of Biochemistry, 4University of Istanbul, Istanbul Medical School, Department of Dermatology Behcet's Disease (BD) is a systemic inflammatory disorder of unknown etiology. BD refers to a toad of recurrent oral aphth0us ulcers, genital aphthae, and conjunctivitis. Additional clinical manifestations include arthritis, thrombophiebitis, erythema nodosum-like lesions and neurologic involvement. This diversity makes early diagnosis difficult, however oral lesions are usually the first sign of the disease in
Maxillofacial pathology and medicine
35
70-90% of cases. The lesion is a vasculitis with neutrophilic infiltration. Superoxide radicals, possible mediators for tissue damage in BD, are elevated in neutrophils and plasma. We studied Malonedialdehyde (MDA) levels in the intact and ulcerated mucosal tissues of BD patients. The attack of biomembranes by oxygen radicals produces MDA via lipid peroxidation. Patients with clinically active disease were included and classified according to the presence of oral lesions. Intact and ulcerated oral mucosa of patients and healthy subjects were biopsied. MDA levels were measured using 2-Thiobarbituric acid (TBA) method. In healthy subjects (n=lS), MDA level was 1.63-+0.82 mnol/mg protein, in Behcet's group (n=25) it was 2.06-+ 1.23. Results were analyzed using Mean Whitney U test. The difference between the groups was significant (z=4.57, P<0.001). MDA levels of BD patients with (n= 17) and without lesion (n = 8) were 1.97-+ 1.34 and 2.03 -+1.11, respectively. MDA levels were slightly higher in the group without lesion, but the difference was not statistically significant. There was no correlation between MDA level and oral lesions. However, increased MDA levels in the mucosa is commensurate with increased superoxide radical generation in BD.
8. The Use of Liquid Nitrogen Cryotherapy in the Management of Jaw Lesions
Schmidt, B., Pogrel, M. University of California, San Francisco Purpose To evaluate the use of enucleation and cryosurgery in the management of locally 'aggressive jaw lesions. Significance A number of locally aggressive jaw lesions occur the oral and maxillofacial region. These lesions, including ameloblastoma, odontogenic keratocyst (OKC), myxomas and giant cell lesions, have a high incidence of recurrence following enucleation alone. Liquid nitrogen cryotherapy offers the unique advantage of killing cells within the bone while leaving the osseous framework intact 1. Encouraging results have been produced with the use and cryotherapy in the management of these lesions 2. The purpose of this study was tO evaluate the use of enucleation and cryosurgery with liquid nitrogen in the management of locally aggressive jaw lesions'. Materials and methods The design of the study was a retrospective review. 30 patients with locally aggressive jaw lesions were treated. The cases consisted of 26 OKCs, 3 myxomas and one giant cell lesion. All patients were treated with a combination of enucleation and cryosurgery. Postoperative follow-up consisted of clinical and radiographic examinations. Results A total of 30 patients were treated with enucleation and cryotherapy; 15 of the patients were male and 15 were fe-
Kimura, H., Akanuma, M., Kusumi, A. Department of Dentistry & Oral Surgery, Hirosaki University School of Medicine To investigate the role of inflammatory cytokines in jaw cyst enlargement, the amount of interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-c0 in cyst walls were measured by enzyme-linked immunosorbent assay (ELISA). Furthermore, the localization of both cytokines in cyst wall was investigated by immunohistochemical method. Sixty-five cyst walls, including 14 of dentigerous cyst, 38 of radicular cyst and 13 of postoperative maxillary cyst, and 10 normal gingivae were used as samples. The amount and the positive staining ratio of cytokines were estimated according to the degree of inflammation determined by histological findings in H-E stained section of the same cyst wall. Both cytokines were detected in 41 cyst walls, but the range of values was very wide from 32 to 5034 pg/g wet tissue (IL-6) and from 46 to 1484 pg/g wet tissue (TNF-c0. However, the amount of cytokines reflected the degree of inflammation of cyst wall and there was a significant (P<0.01, n=33) positive correlation between IL-6 and TNFc~in the same samples. According to the classification of jaw cysts, no significant differences were found in the amount of cytokines. Positive stainings for IL-6 and TNF-c~ were observed at the almost same site in cyst wall, that is, at fibrous tissues, epithelial cells, inflammatory cells and blood capillaries. The positive staining ratio of fibrous tissues showed the highest value among those described organs. These findings suggest that both IL-6 and TNF-c~ produced in cyst wall are deeply involved in the bone resorption and the enlargement of jaw cysts.
7. Oral Mucosal Malonedialdehyde (MDA) Levels in Behcet's Patients With and Without Oral Lesions
A. Kaynar, A. 1, Guven, yz, Uslu, E. 3, Yildiz, E. 2, Sarioglu, S. 2, Kose, A. 4, Oner, B. a, Aybar, B. 1, Sarica, R. 4, Azizierli, G.4 Univ~ersity of Istanbul, School of Dentistry, Department of Oral !Surgery, 2 University of Istanbul, School of Dentistry, Dep@tment of Biochemistry, 3University of Istanbul, Cerrahpasa Medical School, Department of Biochemistry, 4University of Istanbul, Istanbul Medical School, Department of Dermatology Behcet's Disease (BD) is a systemic inflammatory disorder of unknown etiology. BD refers to a toad of recurrent oral aphth0us ulcers, genital aphthae, and conjunctivitis. Additional clinical manifestations include arthritis, thrombophiebitis, erythema nodosum-like lesions and neurologic involvement. This diversity makes early diagnosis difficult, however oral lesions are usually the first sign of the disease in
Maxillofacial pathology and medicine
35
70-90% of cases. The lesion is a vasculitis with neutrophilic infiltration. Superoxide radicals, possible mediators for tissue damage in BD, are elevated in neutrophils and plasma. We studied Malonedialdehyde (MDA) levels in the intact and ulcerated mucosal tissues of BD patients. The attack of biomembranes by oxygen radicals produces MDA via lipid peroxidation. Patients with clinically active disease were included and classified according to the presence of oral lesions. Intact and ulcerated oral mucosa of patients and healthy subjects were biopsied. MDA levels were measured using 2-Thiobarbituric acid (TBA) method. In healthy subjects (n=lS), MDA level was 1.63-+0.82 mnol/mg protein, in Behcet's group (n=25) it was 2.06-+ 1.23. Results were analyzed using Mean Whitney U test. The difference between the groups was significant (z=4.57, P<0.001). MDA levels of BD patients with (n= 17) and without lesion (n = 8) were 1.97-+ 1.34 and 2.03 -+1.11, respectively. MDA levels were slightly higher in the group without lesion, but the difference was not statistically significant. There was no correlation between MDA level and oral lesions. However, increased MDA levels in the mucosa is commensurate with increased superoxide radical generation in BD.
8. The Use of Liquid Nitrogen Cryotherapy in the Management of Jaw Lesions
Schmidt, B., Pogrel, M. University of California, San Francisco Purpose To evaluate the use of enucleation and cryosurgery in the management of locally 'aggressive jaw lesions. Significance A number of locally aggressive jaw lesions occur the oral and maxillofacial region. These lesions, including ameloblastoma, odontogenic keratocyst (OKC), myxomas and giant cell lesions, have a high incidence of recurrence following enucleation alone. Liquid nitrogen cryotherapy offers the unique advantage of killing cells within the bone while leaving the osseous framework intact 1. Encouraging results have been produced with the use and cryotherapy in the management of these lesions 2. The purpose of this study was tO evaluate the use of enucleation and cryosurgery with liquid nitrogen in the management of locally aggressive jaw lesions'. Materials and methods The design of the study was a retrospective review. 30 patients with locally aggressive jaw lesions were treated. The cases consisted of 26 OKCs, 3 myxomas and one giant cell lesion. All patients were treated with a combination of enucleation and cryosurgery. Postoperative follow-up consisted of clinical and radiographic examinations. Results A total of 30 patients were treated with enucleation and cryotherapy; 15 of the patients were male and 15 were fe-