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Subcutaneous Embryonal Rhabdomyosarcoma in a Dog
280
ESVP/ECVP Proceedings 2009
141:4, 2009
9
10
SUBCUTANEOUS EMBRYONAL RHABDOMYOSARCOMA IN A DOG N. Pinto da Cunha *, C. Palmieri y, L. Della Salda y, D. Stefanello z, P. Roccabianca *, M. Caniatti * and G. Avallone Giancarlo* *DIPAV, University of Milan, zDSC, University of Milan and yDCBS, University of Teramo, Italy Introduction: Canine rhabdomyosarcomas (RBDs) are classified into embryonal, botryoid, alveolar and pleomorphic forms. Although rare, the embryonal type is the most common and is reported to have a locally invasive behaviour and metastatic potential. Materials and Methods: An 11-year-old pointer had a subcutaneous mass in the left forearm and an enlarged prescapular lymph node. Cytology, immunocytochemistry (ICC), histology and electron microscopy (EM) were performed. Results: Cytology of the mass and lymph node showed neoplastic cells with morphological features consistent with an undifferentiated sarcoma. Cytological atypia was severe. Histology revealed a subcutaneous, unencapsulated neoplasm composed of sheets of cells with the same morphology observed in aspirates. The lymph node was positive for metastasis. Expression of vimentin, desmin and myoglobin was demonstrated using ICC and indicated a diagnosis of RBD. EM revealed numerous mitochondria, large nuclei and nucleoli and rare dense masses of tangled myofilaments, suggestive of poorly differentiated rhabdomyoblasts. The findings were consistent with an embryonal RBD. Six months after treatment the dog remains free of disease. Conclusions: Cytology and ICC are pivotal for the diagnosis of RBD. Despite the guarded prognosis, in this case of metastatic embryonal RBD the dog responded well to therapy.
ENCEPHALOMALACIA AND INTRAHEPATIC CHOLANGIOCELLULAR CARCINOMA IN A BUDGERIGAR (MELOPSITTACUS UNDULATUS) H. Azarabad *, M. Nouri y, F. Sasani * and S.H. Marjanmehr* *University of Tehran, Department of Veterinary Pathology, Tehran and y Veterinary Practitioner, Tehran, Iran Introduction: This is believed to be the first report of encephalomalacia associated with intrahepatic bile duct carcinoma in an adult budgerigar. Materials and Methods: An adult male budgerigar was presented for necropsy examination in May 2009 with a history of ataxia and star gazing of several days duration. The liver was pale and enlarged and two pale areas were detected in the cortex of the cerebrum. Representative samples from all organs were fixed in formalin and stained with haematoxylin and eosin. Results: Histological examination of the liver showed numerous epithelial lined ducts with an associated dense fibrous stroma. Based on morphological criteria, the tumour was classified as a low-grade bile duct carcinoma. In the cerebrum, spongiform change in the gray matter was accompanied by status spongiosus in the white matter. Perivascular and perineuronal spaces were dilated. Conclusions: A case of encephalomalacia and intrahepatic bile duct carcinoma in an adult male budgerigar is reported. Vitamin E deficiency may have contributed to the development of encephalomalacia in this bird.
11 PROGNOSTIC VALUE OF INTRATUMOURAL VESSEL DENSITY IN CUTANEOUS MAST CELL TUMOURS I. Badurek *, M. Sobczak-Filipiak *, M. Wo´dkiewicz y, M. Skrzypczak * and E. Malicka* *Warsaw University of Life Sciences and yUniversity of Warsaw, Poland Introduction: Mast cell tumours (MCTs) are amongst the most common skin tumours of dogs. The current grading system established by Patanik et al. (1984) is based on histomorphological features. The main disadvantage of the system relates to standardization of histological criteria, especially for grade II MCT. The purpose of this study was to determine whether microvessel density is a useful supplementary marker to Patnaik’s system. Materials and Methods: Paraffin wax-embedded tissue sections obtained from 65 cases of MCT were stained with haematoxylin and eosin, toluidine blue and classified according to Patnaik’s grading system. Assessment of microvessel density was performed in sections immunolabelled for expression of factor VIII related antigen according to Weidner et al. (1991). Results: Of the 65 tumours sampled, 19 were grade I, 31 were grade II and 15 were grade III. No significant correlation was observed between Patnaik’s histopathological grade and microvessel density (Spearman R 5 0.03, P O 0.8). There was no significant difference in microvessel density between tumours of different grade (ANOVA P O 0.1). Conclusions: Measurement of tumour angiogensis is not a useful additional tool in histomorphological assessment of canine MCT. The release of angiogenic factors contained in secretory granules of neoplastic mast cells is being investigated.
12 RECIPROCAL RELATIONSHIP OF BLOOD PROFILES AS AN IMMUNOLOGICAL BIOINDICATOR IN GREAT STURGEON (HUSO HUSO) T. Bagheri * and A. Hedayati*,y *Department of Fishery, Gorgan University of Agricultural Science, Gorgan and yDepartment of Marine Biology, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran Introduction: Recognition of blood profiles and awareness of ongoing related reciprocal relationships is important in aquaculture, especially in diagnosis of disease. The aim of this study was to measure blood profiles in 4 to 5-year-old great sturgeon cultured in brackish water pools in BAFGH-Iran. Materials and Methods: Blood samples were collected from the caudal vein every 3 months and plasma was frozen for later analysis. Hormone levels were measured by radioimmunoassay (RIA), glucose with an autoanalyser, calcium and magnesium with a spectrophotometer and sodium and potassium with a film photometer. Results and Conclusions: Significant correlations were detected between the following biochemical parameters: glucose/calcium, sodium/potassium, sodium/magnesium, potassium/calcium and potassium/magnesium. Of the hormonal profiles, testosterone/oestradiol had significant direct correlation. Significant correlations also occurred between cortisol and glucose, and between sodium and potassium. Levels of cortisol showed no correlation with those of other hormones. Blood profiles in great sturgeon are similar to those reported in other sturgeon.
ESVP/ECVP Proceedings 2009
141:4, 2009
9
10
SUBCUTANEOUS EMBRYONAL RHABDOMYOSARCOMA IN A DOG N. Pinto da Cunha *, C. Palmieri y, L. Della Salda y, D. Stefanello z, P. Roccabianca *, M. Caniatti * and G. Avallone Giancarlo* *DIPAV, University of Milan, zDSC, University of Milan and yDCBS, University of Teramo, Italy Introduction: Canine rhabdomyosarcomas (RBDs) are classified into embryonal, botryoid, alveolar and pleomorphic forms. Although rare, the embryonal type is the most common and is reported to have a locally invasive behaviour and metastatic potential. Materials and Methods: An 11-year-old pointer had a subcutaneous mass in the left forearm and an enlarged prescapular lymph node. Cytology, immunocytochemistry (ICC), histology and electron microscopy (EM) were performed. Results: Cytology of the mass and lymph node showed neoplastic cells with morphological features consistent with an undifferentiated sarcoma. Cytological atypia was severe. Histology revealed a subcutaneous, unencapsulated neoplasm composed of sheets of cells with the same morphology observed in aspirates. The lymph node was positive for metastasis. Expression of vimentin, desmin and myoglobin was demonstrated using ICC and indicated a diagnosis of RBD. EM revealed numerous mitochondria, large nuclei and nucleoli and rare dense masses of tangled myofilaments, suggestive of poorly differentiated rhabdomyoblasts. The findings were consistent with an embryonal RBD. Six months after treatment the dog remains free of disease. Conclusions: Cytology and ICC are pivotal for the diagnosis of RBD. Despite the guarded prognosis, in this case of metastatic embryonal RBD the dog responded well to therapy.
ENCEPHALOMALACIA AND INTRAHEPATIC CHOLANGIOCELLULAR CARCINOMA IN A BUDGERIGAR (MELOPSITTACUS UNDULATUS) H. Azarabad *, M. Nouri y, F. Sasani * and S.H. Marjanmehr* *University of Tehran, Department of Veterinary Pathology, Tehran and y Veterinary Practitioner, Tehran, Iran Introduction: This is believed to be the first report of encephalomalacia associated with intrahepatic bile duct carcinoma in an adult budgerigar. Materials and Methods: An adult male budgerigar was presented for necropsy examination in May 2009 with a history of ataxia and star gazing of several days duration. The liver was pale and enlarged and two pale areas were detected in the cortex of the cerebrum. Representative samples from all organs were fixed in formalin and stained with haematoxylin and eosin. Results: Histological examination of the liver showed numerous epithelial lined ducts with an associated dense fibrous stroma. Based on morphological criteria, the tumour was classified as a low-grade bile duct carcinoma. In the cerebrum, spongiform change in the gray matter was accompanied by status spongiosus in the white matter. Perivascular and perineuronal spaces were dilated. Conclusions: A case of encephalomalacia and intrahepatic bile duct carcinoma in an adult male budgerigar is reported. Vitamin E deficiency may have contributed to the development of encephalomalacia in this bird.
11 PROGNOSTIC VALUE OF INTRATUMOURAL VESSEL DENSITY IN CUTANEOUS MAST CELL TUMOURS I. Badurek *, M. Sobczak-Filipiak *, M. Wo´dkiewicz y, M. Skrzypczak * and E. Malicka* *Warsaw University of Life Sciences and yUniversity of Warsaw, Poland Introduction: Mast cell tumours (MCTs) are amongst the most common skin tumours of dogs. The current grading system established by Patanik et al. (1984) is based on histomorphological features. The main disadvantage of the system relates to standardization of histological criteria, especially for grade II MCT. The purpose of this study was to determine whether microvessel density is a useful supplementary marker to Patnaik’s system. Materials and Methods: Paraffin wax-embedded tissue sections obtained from 65 cases of MCT were stained with haematoxylin and eosin, toluidine blue and classified according to Patnaik’s grading system. Assessment of microvessel density was performed in sections immunolabelled for expression of factor VIII related antigen according to Weidner et al. (1991). Results: Of the 65 tumours sampled, 19 were grade I, 31 were grade II and 15 were grade III. No significant correlation was observed between Patnaik’s histopathological grade and microvessel density (Spearman R 5 0.03, P O 0.8). There was no significant difference in microvessel density between tumours of different grade (ANOVA P O 0.1). Conclusions: Measurement of tumour angiogensis is not a useful additional tool in histomorphological assessment of canine MCT. The release of angiogenic factors contained in secretory granules of neoplastic mast cells is being investigated.
12 RECIPROCAL RELATIONSHIP OF BLOOD PROFILES AS AN IMMUNOLOGICAL BIOINDICATOR IN GREAT STURGEON (HUSO HUSO) T. Bagheri * and A. Hedayati*,y *Department of Fishery, Gorgan University of Agricultural Science, Gorgan and yDepartment of Marine Biology, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran Introduction: Recognition of blood profiles and awareness of ongoing related reciprocal relationships is important in aquaculture, especially in diagnosis of disease. The aim of this study was to measure blood profiles in 4 to 5-year-old great sturgeon cultured in brackish water pools in BAFGH-Iran. Materials and Methods: Blood samples were collected from the caudal vein every 3 months and plasma was frozen for later analysis. Hormone levels were measured by radioimmunoassay (RIA), glucose with an autoanalyser, calcium and magnesium with a spectrophotometer and sodium and potassium with a film photometer. Results and Conclusions: Significant correlations were detected between the following biochemical parameters: glucose/calcium, sodium/potassium, sodium/magnesium, potassium/calcium and potassium/magnesium. Of the hormonal profiles, testosterone/oestradiol had significant direct correlation. Significant correlations also occurred between cortisol and glucose, and between sodium and potassium. Levels of cortisol showed no correlation with those of other hormones. Blood profiles in great sturgeon are similar to those reported in other sturgeon.