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Subject index for volume 28
CRYOBIOLOGY
28,
605-613 (191)
Subject index for Volume 28 A
Abstracts papers presented at 28th Annual Meeting of the Society for Cryobiology, 511 Amino acid composition ice nucleator protein (Melampus bidentatus), 483 Amino acids excitatory, role in spinal cord seizures evoked by sudden cooling (frog, toad), 255 Annealing temperature, solidified aqueous solutions of glycerol, analysis, 268 Arteries femoral, reactive properties, intrinsic alteration during hypothermia (rabbit), 499 Autotomy development after peripheral cryogenic nerve lesion (rat), 460 B
Bacteria Escherichia coli HBlOl, with recombinant plasmid, viability and genetic stability, effects of preservation methods, 251 Biotransformation in liver slices, effects of vitrification (human), 216 Blastogenesis lymphocytes, effect of cryopreservation (pig), 405 Book reviews Low Temperature Biotechnology: Emerging Applications and Engineering Contributions. J. J. McGrath and K. R. Diller (Eds.), 1988,203 Quantitative Cryofixation. W. B. Bald, 1987, 302
Calcium effect on stability of phosphatidylcholine sonicated vesicles during freeze-thaw cycles, 279 trehalose cryoprotection of phosphatidylcholine vesicles, 347 free intracelhdar, lymphocytes, effect of cryopreservation (pig), 405 uptake and accumulation in cardiac sarcoplasmic reticulum, effects of hibernation (ground squirrel), 354 Calcium currents and sodium currents, in ventricular muscle, temperature effects (hedgehog, rat), %
Calorimetry differential scanning, see Differential scanning calorimetry Cell swelling dilution-associated, after freeze-thawing, prevention by sorbitol (human erythrocytes), 237 hypothermia-induced, suppression in cold-stored liver, functional effects (rat), 150 Chlorpromazine effect as donor pretreatment in liver preservation (rat), 422 Cold injury acute inflammatory response of pawskin, inhibition by corticotropin-releasing factor (rat), 185 nonfreezing, associated degradation of membrane phospholipids, protection by quinacrine (rabbit leg), 177 Cold storage associated depletion of tissue and cellular ghrtathione (rabbit), 143 hepatocytes, lipid peroxidation, effect of polyethylene glycol (rat), 1 liver effects of sugars in preservation solution (rat), 428 fasting effects (rabbit), 227 subsequent hypothermia-induced cell swelling, suppression: functional effects (rat), 150 Collagen denatured, gel structure at low temperature, effects of cryosolvents, 335 Computer applications determination of optimal location for cooling rate measurements in extended freezing sample, 115 Cooling to heat of fusion, and subsequent rapid rewarming, effects on microvascular corrosion casts (rat hindpaw), 294 super-, see Supercooling Cooling rate effect on intracellular ice formation in hepatocytes (rat), 436 in extended freezing sample, determination of specific location for, computer simulations, 115 solidified aqueous solutions of glycerol, analysis, 268 sudden, evoked spinal cord seizures, role of excitatory amino acids (frog, toad), 255 Cornea effects of propane-1,Zdiol in vitritication solution (rabbit), Vol. 27, p. 42; erratum, 509 605 OOll-2240/91 $3.00 Coewiaht 0 1991 by Academic Press, Inc. Au&l& of reprcd~ction in any form nservd
606
SUBJECT
endothelium, effects of equimolar combination of propane-1,Zdiol and glycerol (rabbit), 314 tolerance to vitrifiable concentrations of propane1,2-diol (rabbit), 159; errctl(M, 509 Corrosion casts microvascular, effects of cooling to heat of fusion and subsequent rapid rewarming (rat hindpaw), 294 Corticotropin-releasing factor inhibition of acute inflammatory response of pawskin to cold injury (rat), 185 Cryoanalgesia effects on behavior and spinal substance P (rat), 460 Cryomicroscopy in analysis of membrane osmotic properties of monocytes at subfreezing temperatures (human), 391 in characterization of intracellular ice formation during freezing of oocytes without cryoadditives (mouse), 55 in hepatocytes (rat), 436 Cryopreservation heart, by freezing at high subzero temperatures (rat), 171 liver slices (human, pig), 131 lymphocytes, effect on in vitro reactivity (pig), 405 pea shoot tips in cryoprotectant-supplemented medium, survival, effects of days of culture and terminal freezing temperature, 288 semen, cryosurvival of Trichomonas vaginalis during, evaluation (human), 246 and storage, Escherichia coli HBlOl with recombinant plasmid, effects on viability and genetic stability, 251 Cryoprotectants in combination with vehicle solutions, comparative effects on cultured vascular endothelial cells (pig), 413 Cryoprotection hepatocytes: intracellular ice formation under various chemical and physical conditions (rat), 436 phosphatidylcholine vesicles effects of carbohydrate derivatives, 327 by trehalose, effect of Ca*‘, 347 phospholipid bilayers by dimethyl Sulfoxide, mechanism, 467 Crystallization temperature in analysis of tissue distribution of ice-nucleating agents in larvae (rice stem borer), 376 Culture media cryoprotectant-supplemented, pea shoot tips cryopreserved in, survival: effect of days of culture, 288 Hepes-buffered, effect on survival of hypothermiaexposed V79 cells, 8 Cytotoxicity cell-mediated, lymphocytes, effect of cryopreservation (pig), 405
INDEX
fluorescent light, effect on survival of hypothermiaexposed V79 cells, 8 D
Development vitritied oocytes after in vitro fertilization, effects of dilution methods (mouse), 50 Differential scanning calorimetry in analysis of cryobehavior of solidified aqueous solutions of glycerol, 268 glassy state in vitrified glycerol solutions, 87 structural states of freeze-dried and freezeconcentrated phosphates, 364 Dilution methods associated cell swelling after freeze-thawing, prevention by sorbitol (human erythrocytes), 237 effects on spermatozoal motility in semen frozen and unfrozen in absence of glycerol (sheep), 43 survival of semen frozen in absence of glycerol (sheep), 36 Dimethyl sulfoxide cryoprotective mechanism for phospholipid bilayem, 467 effects on protein synthesis and membrane transport in hepatic mitochondria (rat), 445 and other cryoprotectants, in combination with RPS-2 or HP-5NP vehicle solutions, comparative effects on cultured vascular endothelial cells (pig), 413 in solutions and tissues, quantitation by HPLC (porcine myocardium), 210 DSC, see Differential scanning calorimetry E
Egg yolk in freezing extender, effect on spermatozoal motility in semen frozen and unfrozen in absence of glycerol (sheep), 43 Electron microscopy scanning, in study of structural states of freeze-dried and freeze-concentrated phosphates, 364 Embryo intracellular ice formation, subfreezing volumetric behavior and stochastic modeling (Drosophila melanogaster), 72 Endothelial cells vascular, in vitro effects of cryoprotectants in combination with vehicle solutions (pig), 413 Endothelium comeal, effects of equimolar combination of pro pane-l ,2-diol and glycerol (rabbit), 314 Erythrocytes dilution-associated swelling after freeze-thawing, prevention by sorbitol (human), 237
SUBJECT
suspended in sodium chloride solutions, freeze-thaw injury, effect of initial tonicity (human), 18 Ethanediol and other cryoprotectants, in combination with BPS-2 or HP-SNP vehicle solutions, comparative effects on cultured vascular endothelial cells (pig), 413 Ethylene glycol effects on low temperature gel structure of denatured collagen, 335 Eye, see specific parts F
Fasting effects on quality of liver preservation methods (rabbit), 227 Fertility effects of glycerol in frozen and unfrozen semen (sheep), 36 Fertilization vitrified oocytes in vitro, subsequent development, effects of dilution methods (mouse), 50 Fibroblasts V79 (Chinese hamster lung), survival after exposure to hypothermia, effects of Hepes, free radicals, and H,O, under light and dark conditions, 8 Fluorescent light and dark conditions, effects on survival of hypothermia-exposed V79 cells, 8 Free radicals effects on survival of hypothermia-exposed V79 cells, 8 Freeze-drying phosphate structural states after, SEM and DSC studies, 364 and storage, Escherichia co/i HBlOl with recombinant plasmid, effects on viability and genetic stability, 251 Freeze-thawing cycles, stability of phosphatidylcholine sonicated vesicles during, effect of Ca*+, 279 erythrocytes injury to cells suspended in sodium chloride solutions, effect of initial tonicity (human), 18 subsequent cell swelling after dilution, prevention by sorbitol (human), 237 phosphatidylcholine vesicles cryoprotection by trehalose, effect of Ca2+ , 347 cryoprotective effects of carbohydrate derivatives, 327 Freezing concentrated phosphates, structural states, SEM and DSC studies, 364 foot muscle, analysis of associated damage (Melampus bidentatus), 491 heart at high subzero temperatures for preservation (m), 171
607
INDEX
low-temperature, and storage, Escherichiu coli HBlOl with recombinant plasmid, effects on viability and genetic stability, 251 oocytes without cryoadditives, intracelhdar ice formation during, cryomicroscopic analysis, 55 orange trees, effects on main stem wood, 382 plate-shaped container for, specific location in sample for determination of cooling rate, computer simulations, 115 semen, development of extender: effects of osmotic pressure, egg yolk levels, sugar types, and method of dilution (sheep), 43 slow, associated mechanical stress due to extracellular ice crystal formation, induction of lamellar to hexagonal II phase transitions in hyphal tonoplasts (mushroom), 191 yeast, enhanced survival in cells expressing antifreeze gene analog, 474 Frog isolated spinal cord, seizures evoked by sudden cooling, role of excitatory amino acids, 255 Fungi mushroom, hyphal tonoplasts, lamellar to hexagonal II phase transitions, induction by mechanical stress due to extracellular ice crystal formation, 191 Saccharomyces cerevisiae, expressing antifreeze gene analog, enhanced survival after freezing, 474 G
Galactose cryoprotective effects on phosphatidylcholine vesicles, 327 Gels denatured collagen, structure at low temperature, effects of cryosolvents, 335 Genes antifreeze, analog, yeast expressing, enhancement of survival after freezing, 474 Genetic stability and viability, Escherichiu coli HBlOl with recombinant plasmid, effects of preservation methods, 251 Glass transitions and relaxation, in vitrified glycerol solutions, DSC study, 87 various, solidified aqueous solutions of glycerol, analysis, 268 Glucose in preservation solution, effect on cold-stored liver (rat), 428 Glycerol aqueous solutions, glassy state, DSC study, 87 effect on functional recovery of isolated heart after freezing preservation at high subzero temperatures (rat), 171
SUBJECT INDEX
608
in equimolar combination with propane-l ,Zdiol, effects on cornea (rabbit), 314 in frozen and unfrozen semen, effects on fertility rates (sheep), 36 and other cryoprotectants, in combination with RPS-2 or HP-SNP vehicle solutions, comparative effects on cultured vascular endothelial cells (pig), 413 solidified aqueous solutions, cryobehavior analysis, 268 Glycine prevention of cold ischemic injury in isolated hepatocytes (rat), 105 Ground squirrel cardiac sarcoplasmic reticulum calcium uptake and accumulation, effects of hibernation, 354 H
Heart glutathione depletion in cold storage (rabbit), 143 isolated, freezing preservation at high subzero temperatures (rat), 171 right ventricle, papillary muscle, sodium and calcium currents, temperature effects (hedgehog, rat), 96 sarcoplasmic reticulum, calcium uptake and accumulation, effects of hibernation (ground squirrel), 354 Hedgehog ventricular muscle, sodium and calcium currents, temperature effects, 96 Hepatocytes, see also Liver cold ischemic injury, prevention by glycine (rat), 105 cold-stored, lipid peroxidation, effect of polyethylene glycol (rat), 1 intracelhdar ice formation, characterization by cryomicroscopy (rat), 436 Hibernation effects on calcium uptake and accumulation in cardiac sarcoplasmic reticulum (ground squirrel), 354 High-performance liquid chromatography in quantitation of dimethyl sulfoxide in solutions and tissues (porcine myocardium), 210 Hindpaw microvascular patency after cooling to heat of fusion and subsequent rapid rewarming, evaluation by corrosion casting (rat), 294 HP-SNP and RPS-2, in combination with four cryoprotectants, comparative effects on cultured vascular endothelial cells (pig), 413 Human erythrocytes dilution-associated swelling after freeze-thawing, prevention by sorbitol, 237
suspended in sodium chloride solutions, freezethaw injury, effect of initial tonicity, 18 liver slices biotransformation in, effects of vitrification, 216 cryopreservation, 131 monocytes, membrane osmotic properties at subfreezing temperatures, cryomicroscopic analysis, 391 semen cryopreservation, cryosurvival of Trichomonas vaginalis during, evaluation, 246 Hydrogen peroxide effects on survival of hypothermia-exposed V79 cells, 8 Hypothermia exposed V79 cells, survival, effects of Hepes, free radicals, and H,O, under light and dark conditions, 8 induced cell swelling, suppression in cold-stored liver, functional effects (rat), 150 intrinsic alteration of arterial reactive properties (rabbit), 499
Ice extracellular formation crystals, resulting mechanical stress, induction of lamellar to hexagonal II phase transitions in hyphal tonoplasts (mushroom), 191 in foot muscle, associated damage, analysis (Melampus bidentatus), 491 intracellular formation in embryos: subfreezing volumetric behavior and stochastic modeling (Drosophila melanogaster), 72 in hepatocytes, characterization by cryomicroscopy (rat), 436 in oocytes during freezing without cryoadditives, cryomicroscopic analysis (mouse), 55 nucleation agents, tissue distribution in larvae (rice stem borer), 376 Inflammation pawskin, acute response to cold injury, inhibition by corticotropin-releasing factor (rat), 185 Injury cold, see Cold injury cold ischemic, in isolated hepatocytes, prevention by glycine (rat), 105 freeze-thawing, erythrocytes suspended in sodium chloride solutions, effect of initial tonicity (human), 18 Insects fruitfly (Drosophila melanogaster), embryos, intracellular ice formation: subfreezing volumetric behavior and stochastic modeling, 72 rice stem borer (Chile suppressalis), larvae, tissue distribution of ice-nucleating agents, 376
SUBJECT
Ischemia cold, induced injury in isolated hepatocytes, prevention by glycine (rat), 105 K Kidney glutathione depletion in cold storage (rabbit), 143 L Larvae tissue distribution of ice-nucleating agents (rice stem borer), 376 L-33 tissue membrane phospholipids, nonfreezing cold injury-associated degradation, protection by quinacrine (rabbit), 177 Light fluorescent, see Fluorescent light Lipid peroxidation in cold-stored hepatocytes, effect of polyethylene glYCO1(rat), 1 Liposomes cryoprotective mechanism of dimethyl sulfoxide, 467 Liver, see also Hepatocytes cold-stored, effects of sugars in preservation solution (rat), 428 isolated perfused, preservation, effects of donor pretreatment with chlorpromazine, quinacrine, and verapamil (rat), 422 mitochondria, protein synthesis and membrane transport, effects of cryopreservation (rat), 445 preservation by various methods, effects of fasting (rabbit), 227 preserved by cold storage, functional effects of sup pression of hypothermia-induced cell swelling (rat), 150 slices biotransformation in, effects of vitrification (human), 216 cryopreservation (human, pig), 131 tissue and cellular glutathione depletion in cold storage (rabbit, rat), 143 Lymphocytes fresh and cryopreserved, in vitro reactivity, comparison (pig), 405
INDEX
609
phospholipid bilayer, cryoprotective mechanism of dimethyl sulfoxide, 467 Membrane transport in hepatic mitochondria, effect of cryopreservation (rat), 445 Methanol effects on low temperature gel structure of denatured collagen, 335 Mitochondria hepatic, protein synthesis and membrane transport, effects of cryopreservation (rat), 445 Models mathematical, heat transfer: optimal location of thermal sensor, 115 stochastic, intracellular ice formation in embryos (Drosophila melanogaster), 72 Monocytes membrane osmotic properties at subfreezing temperatures, cryomicroscopic analysis (human), 391 Motility spermatozoa in semen frozen and unfrozen in absence of glycerol, effects of dilution methods, osmolality, egg yolk levels, and sugar types (sheep), 43 Mouse oocytes intracellular ice formation during freezing without cryoadditives, cryomicroscopic analysis, 55 vitrified, development after in vitro fertilization, effects of dilution methods, 50 Muscle foot, ice formation and freezing damage (Melampus bidentatus), 491 ventricular, sodium and calcium currents, temperature effects (hedgehog, rat), % N Nerves
sciatic, autotomy and decrease in spinal substance P after cryolesioning (rat), 460 Norepinephrine arterial responsiveness, effects of hypothermia (rabbit), 499 0
Maltose cryoprotective effects on phosphatidylcholine vesicles, 327 Membranes monocyte, osmotic properties at subfreezing temperatures, cryomicroscopic analysis (human), 391 phosphatidylcholine vesicle, cryoprotective effects of carbohydrate derivatives, 327
Obituaries Ronald I. N. Greaves, 307 Jacob Levitt, 111 Vernon P. Perry, 207 Oocytes intracellular ice formation during freezing without cryoadditives, cryomicroscopic analysis (mouse), 55 vitrified, development after in vitro fertilization, effects of dilution methods (mouse), 50
SUBJECT INDEX
610
Osmolality freezing extender, effect on spermatozoal motility in semen frozen and unfrozen in absence of glycerol (sheep), 43 ovary thecal gland, seasonal changes (ground squirrel), 503 P
Perfusion continuous, liver, effects of fasting (rabbit), 227 Permeability osmotic, monocyte membranes at subfreezing temperatures, cryomicroscopic analysis (human), 391 Phase transitions lamellar to hexagonal II, in hyphal tonoplasts, induction by mechanical stress due to extracellular ice crystal formation (mushroom), 191 Phosphates freeze-concentrated and freeze-dried, structural states, SEM and DSC studies, 364 Phosphatidylcholine vesicles cryoprotection by carbohydrate derivatives, 327 trehalose, effect of Ca”, 347 sonicated, stability during freeze-thaw cycles, effect of Ca2+, 279 Phospholipids bilayers, cryoprotective mechanism of dimethyl sulfoxide, 467 membrane, nonfreezing cold injury-associated degradation, protection by quinacrine (rabbit leg), 177 pig liver slices, cryopreservation, 131 lymphocytes, fresh and cryopreserved, in vitro reactivity, comparison, 405 myocardium, dimethyl sulfoxide levels, HPLC assay, 210 sperm, cryopreserved, viability assessment by thermal stress test, 454 vascular endothelial cells in vitro, effects of cryoprotectants in combination with vehicle solutions, 413 Plants orange (Citrus sinensis), main stem wood, changes during supercooling and freezing, 382 pea (Pisum sativum), cryopreserved shoot tips, survival, effects of number of days in cryoprotectant-supplemented culture medium and terminal freezing temperature, 288 Plasmids recombinant, in Escherichia coli HBIOl, viability and genetic stability, effects of preservation methods, 251
Polyethylene glycol effect on lipid peroxidation in cold-stored hepatocytes (rat), 1 Preservation cryo-, see Cryopreservation liver, cold-stored, effects of donor pretreatment with chlorpromazine, quinacrine, and verapamil (rat), 422 Preservation solutions compositional changes, suppression of hypothermia-induced cell swelling in cold-stored liver (rat), 150 sugars in, effects on cold-stored liver (rat), 428 Propane-l ,2-diol, see Propylene glycol Propylene glycol in equimolar combination with glycerol, effects on cornea (rabbit), 314 and other cryoprotectants, in combination with RPS-2 or HP-5NP vehicle solutions, comparative effects on cultured vascular endothelial cells (pig), 413 vitrifiable concentrations, cornea1 tolerance (rabbit), 159; erratum, 509 in vitrification solution, effect on corneas (rabbit), Vol. 27, p. 42; erratum, 509 Proteins antifreeze, encoding gene analog, yeast expressing, enhanced survival after freezing, 474 ice nucleator, purification and partial characterization (Melampus bidentarus), 483 synthesis in hepatic mitochondria, effect of cryopreservation (rat), 445 Protozoa Trichomonas vaginalis, cryosurvival during cryopreservation of semen, evaluation (human), 246 Q Quinacrine effect as donor pretreatment in liver preservation (rat), 422 protection of membrane phospholipids from nonfreezing cold injury-associated degradation (rabbit leg), 177 R
Rabbit cornea effects of propane-1,2-diol in vitrification solution, Vol. 27, p. 42; erratum, 509 endothelium, effects of equimolar combination of propane-l ,2-dial and glycerol, 3 14 tolerance to vitrifiable concentrations of propanel,Zdiol, 159; erratum, 509 femoral artery, reactive properties, intrinsic alteration during hypothermia, 499 leg tissue, nonfreezing cold injury, protection by quinacrine, 177
SUBJECT
liver, quality of preservation methods, effects of fasting, 227 tissue glutathione, effects of cold storage, 143 Raffinose in preservation solution, effect on cold-stored liver (rat), 428 Rat heart, isolated, freezing preservation at high subzero temperatures, 171 hepatocytes cold ischemic injury, prevention by glycine, 105 cold-stored, lipid peroxidation, effect of polyethylene glycol, 1 glutathione depletion in cold storage, analysis, 143 intracellular ice formation, characterization by cryomicroscopy, 436 hindpaw, microvascular patency after cooling to heat of fusion and subsequent rapid rewarming, evaluation by corrosion casting, 294 liver cold-stored effects of sugars in preservation solution, 428 hypothermia-induced cell swelling, suppression: functional effects, 150 isolated perfused, preservation, effects of donor pretreatment with quinacrine, chlorpromazine, and verapamil, 422 mitochondria, hepatic, protein synthesis and membrane transport in, effects of cryopreservation, 445 pawskin, acute inflammatory response to cold injury, inhibition by corticotropin-releasing factor, 185 sciatic nerve cryolesion, subsequent autotomy and decrease in spinal substance P, 460 thyrotropin and thyroid hormones, effects of exposure to chronic-intermittent cold, 400 ventricular muscle, sodium and calcium currents, temperature effects, 96 Rewarming rate rapid, after cooling to heat of fusion, effects on microvascular corrosion casts (rat hindpaw), 294 RPS-2 and HP-SNP, in combination with four cryoprotectants, comparative effects on cultured vascular endothelial cells (pig), 413 S Sarcoplasmic reticulum cardiac, calcium uptake and accumulation, effects of hibernation (ground squirrel), 354 Seasonal change in ovarian thecal gland (ground squirrel), 503 Seizures spinal cord, evoked by sudden cooling, role of excitatory amino acids (frog, toad), 255
INDEX
611
Semen cryopreservation. cryosurvival of Trichomonas vagin& during, evaluation (human), 246 frozen and unfrozen in absence of glycerol: effects of osmolahty, egg yolk levels, sugar types, and method of dilution (sheep), 43 in presence or absence of glycerol, analysis: fertility trials and effects of dilution methods (sheep), 36 Sheep semen, frozen and unfrozen in absence of glycerol: effects of osmolahty, egg yolk levels, sugar types, and method of dilution, 43 in presence or absence of glycerol, analysis: fertility trials and effects of dilution methods, 36 Shoot tips cryopreserved, survival, effects of number of days in cryoprotectant-supplemented culture medium and terminal freezing temperature (pea), 288 Skin paw, acute inflammatory response to cold injury, inhibition by corticotropin-releasing factor (rat), 185 Snail foot muscle, ice formation and freezing damage, 491 ice nucleator protein, purification and partial characterization, 483 Sodium chloride solutions, erythrocytes suspended in, freeze-thaw injury, effect of initial tonicity (human), 18 Sodium currents and calcium currents, in ventricular muscle, temperature effects (hedgehog, rat), % Solutions aqueous glycerol, solidified, cryobehavior analysis, 268 vitrified glycerol, DSC study, 87 cryopreservative, dimethyl sulfoxide in, quantitation by HPLC, 210 preservation, see Preservation solutions sodium chloride, erythrocytes suspended in, freezethaw injury, effect of initial tonicity (human), 18 vehicle, in combination with cryoprotectants, comparative effects on cultured vascular endothelial cells (pig), 413 vitritication, see Vitrification solutions Sorbitol prevention of dilution-associated cell swelling after freeze-thawing (human erythrocytes), 237 Spermatozoa cryopreserved, viability assessment by thermal stress test (pig), 454 Spinal cord isolated, seizures evoked by sudden cooling, role of excitatory amino acids (frog, toad), 255
612
SUBJECT INDEX
substance P content, decrease after peripheral cryogenic nerve lesion (rat), 460 Squirrel ovarian thecal gland, seasonal changes, 503 Starvation effect on crystallization temperature of larval tissues (rice stem borer), 376 Storage cold, see Cold storage long-term, Escherichia coli HBlOl with recombinant plasmid preserved by different methods, effects on viability and genetic stability, 251 Stress mechanical, due to extracellular ice crystal formation, induction of lamellar to hexagonal II phase transitions in hyphal tonoplasts (mushroom), 191 Stress test thermal, in assessmet of viability of cryopreserved sperm (Pig), 454 Structural states freeze-concentrated and freeze-dried phosphates, SEM and DSC studies, 364 Substance P spinal content, decrease after peripheral cryogenic nerve lesion (rat), 460 Sucrose in preservation solution, effect on cold-stored liver (rat), 428 sugars various types in freezing extender, effect on spermatozoal motility in semen frozen and unfrozen in absence of glycerol (sheep), 43 Supercooling effects on main stem wood (orange), 382 Survival pea shoot tips cryopreserved in cryoprotectantsupplemented medium, effects of days of culture and terminal freezing temperature, 288 spermatozoa in semen frozen in absence of glycerol, effects of dilution methods (sheep), 36 Trichomonas vaginalis in semen during cryopreservation, evaluation (human), 246 V79 cells after exposure to hypothermia, effects of Hepes, free radicals, and H,O, under light and dark conditions, 8 yeast expressing antifreeze gene analog, enhancement after freezing, 474
T Temperature annealing, solidified aqueous solutions of glycerol, analysis, 268 effects on sodium and calcium currents in ventricular muscle (hedgehog, rat), 96
low chronic-intermittent exposure, effects on thyrotropin and thyroid hormones (rat), 400 denatured collagen gel structure at, effects of cryosolvents, 335 subfreezing, monocyte membrane osmotic properties at, cryomicroscopic analysis (human), 391 terminal freezing, effect on survival of pea shoot tips cryopreserved in cryoprotectant-supplemented medium, 288 Thecal gland ovarian, seasonal changes (ground squirrel), 503 Thyrotropin effects of chronic-intermittent cold (rat), 400 Thyroxine effects of chronic-intermittent cold (rat), 400 Tissues dimethyl sulfoxide levels, HPLC assay, 210 distribution of ice-nucleating agents in larvae (rice stem borer), 376 Toad isolated spinal cord, seizures evoked by sudden cooling, role of excitatory amino acids, 255 Tonicity initial, sodium chloride solutions, effect on freezethaw injury to suspended erythrocytes (human), 18 Tonoplasts hyphal, lamellar to hexagonal II phase transitions, induction by mechanical stress due to extracellular ice crystal formation (mushroom), 191 Trehalose cryoprotection of phosphatidylcholine vesicles, effect of Ca*+ , 347 Triiodothyronine effects of chronic-intermittent cold (rat), 400 V
Verapamil effect as donor pretreatment in liver preservation (rat), 422 Vesicles phosphatidylcholine, see Phosphatidylcholine vesicles Viability cryopreserved sperm, assessment by thermal stress test (pi), 454 and genetic stability, Escherichia coli HBlOl with recombinant plasmid, effects of preservation methods, 251 Vitrification glycerol solutions, glass transition and relaxation, DSC study, 87 liver slices, effects on biotransformation activity (human), 216 oocytes cryopreserved by, development after in
SUBJECT
virro fertilization, effects of dilution methods (mouse), 50 Vitrification solutions propane- 1,Zdiol effect on cornea (rabbit), Vol. 27, p. 42; erratum, 509 equimolar combination with glycerol, effects on cornea (rabbit), 314 in various concentrations, comeal tolerance (rabbit), 159; erratum, 509
613
INDEX
Volumetric behavior subfreezing, during intracellular ice formation in embryos, stochastic modeling (Drosophila melanogaster), 72 W wood
main stem, changes during supercooling and freezing (orange), 382
28,
605-613 (191)
Subject index for Volume 28 A
Abstracts papers presented at 28th Annual Meeting of the Society for Cryobiology, 511 Amino acid composition ice nucleator protein (Melampus bidentatus), 483 Amino acids excitatory, role in spinal cord seizures evoked by sudden cooling (frog, toad), 255 Annealing temperature, solidified aqueous solutions of glycerol, analysis, 268 Arteries femoral, reactive properties, intrinsic alteration during hypothermia (rabbit), 499 Autotomy development after peripheral cryogenic nerve lesion (rat), 460 B
Bacteria Escherichia coli HBlOl, with recombinant plasmid, viability and genetic stability, effects of preservation methods, 251 Biotransformation in liver slices, effects of vitrification (human), 216 Blastogenesis lymphocytes, effect of cryopreservation (pig), 405 Book reviews Low Temperature Biotechnology: Emerging Applications and Engineering Contributions. J. J. McGrath and K. R. Diller (Eds.), 1988,203 Quantitative Cryofixation. W. B. Bald, 1987, 302
Calcium effect on stability of phosphatidylcholine sonicated vesicles during freeze-thaw cycles, 279 trehalose cryoprotection of phosphatidylcholine vesicles, 347 free intracelhdar, lymphocytes, effect of cryopreservation (pig), 405 uptake and accumulation in cardiac sarcoplasmic reticulum, effects of hibernation (ground squirrel), 354 Calcium currents and sodium currents, in ventricular muscle, temperature effects (hedgehog, rat), %
Calorimetry differential scanning, see Differential scanning calorimetry Cell swelling dilution-associated, after freeze-thawing, prevention by sorbitol (human erythrocytes), 237 hypothermia-induced, suppression in cold-stored liver, functional effects (rat), 150 Chlorpromazine effect as donor pretreatment in liver preservation (rat), 422 Cold injury acute inflammatory response of pawskin, inhibition by corticotropin-releasing factor (rat), 185 nonfreezing, associated degradation of membrane phospholipids, protection by quinacrine (rabbit leg), 177 Cold storage associated depletion of tissue and cellular ghrtathione (rabbit), 143 hepatocytes, lipid peroxidation, effect of polyethylene glycol (rat), 1 liver effects of sugars in preservation solution (rat), 428 fasting effects (rabbit), 227 subsequent hypothermia-induced cell swelling, suppression: functional effects (rat), 150 Collagen denatured, gel structure at low temperature, effects of cryosolvents, 335 Computer applications determination of optimal location for cooling rate measurements in extended freezing sample, 115 Cooling to heat of fusion, and subsequent rapid rewarming, effects on microvascular corrosion casts (rat hindpaw), 294 super-, see Supercooling Cooling rate effect on intracellular ice formation in hepatocytes (rat), 436 in extended freezing sample, determination of specific location for, computer simulations, 115 solidified aqueous solutions of glycerol, analysis, 268 sudden, evoked spinal cord seizures, role of excitatory amino acids (frog, toad), 255 Cornea effects of propane-1,Zdiol in vitritication solution (rabbit), Vol. 27, p. 42; erratum, 509 605 OOll-2240/91 $3.00 Coewiaht 0 1991 by Academic Press, Inc. Au&l& of reprcd~ction in any form nservd
606
SUBJECT
endothelium, effects of equimolar combination of propane-1,Zdiol and glycerol (rabbit), 314 tolerance to vitrifiable concentrations of propane1,2-diol (rabbit), 159; errctl(M, 509 Corrosion casts microvascular, effects of cooling to heat of fusion and subsequent rapid rewarming (rat hindpaw), 294 Corticotropin-releasing factor inhibition of acute inflammatory response of pawskin to cold injury (rat), 185 Cryoanalgesia effects on behavior and spinal substance P (rat), 460 Cryomicroscopy in analysis of membrane osmotic properties of monocytes at subfreezing temperatures (human), 391 in characterization of intracellular ice formation during freezing of oocytes without cryoadditives (mouse), 55 in hepatocytes (rat), 436 Cryopreservation heart, by freezing at high subzero temperatures (rat), 171 liver slices (human, pig), 131 lymphocytes, effect on in vitro reactivity (pig), 405 pea shoot tips in cryoprotectant-supplemented medium, survival, effects of days of culture and terminal freezing temperature, 288 semen, cryosurvival of Trichomonas vaginalis during, evaluation (human), 246 and storage, Escherichia coli HBlOl with recombinant plasmid, effects on viability and genetic stability, 251 Cryoprotectants in combination with vehicle solutions, comparative effects on cultured vascular endothelial cells (pig), 413 Cryoprotection hepatocytes: intracellular ice formation under various chemical and physical conditions (rat), 436 phosphatidylcholine vesicles effects of carbohydrate derivatives, 327 by trehalose, effect of Ca*‘, 347 phospholipid bilayers by dimethyl Sulfoxide, mechanism, 467 Crystallization temperature in analysis of tissue distribution of ice-nucleating agents in larvae (rice stem borer), 376 Culture media cryoprotectant-supplemented, pea shoot tips cryopreserved in, survival: effect of days of culture, 288 Hepes-buffered, effect on survival of hypothermiaexposed V79 cells, 8 Cytotoxicity cell-mediated, lymphocytes, effect of cryopreservation (pig), 405
INDEX
fluorescent light, effect on survival of hypothermiaexposed V79 cells, 8 D
Development vitritied oocytes after in vitro fertilization, effects of dilution methods (mouse), 50 Differential scanning calorimetry in analysis of cryobehavior of solidified aqueous solutions of glycerol, 268 glassy state in vitrified glycerol solutions, 87 structural states of freeze-dried and freezeconcentrated phosphates, 364 Dilution methods associated cell swelling after freeze-thawing, prevention by sorbitol (human erythrocytes), 237 effects on spermatozoal motility in semen frozen and unfrozen in absence of glycerol (sheep), 43 survival of semen frozen in absence of glycerol (sheep), 36 Dimethyl sulfoxide cryoprotective mechanism for phospholipid bilayem, 467 effects on protein synthesis and membrane transport in hepatic mitochondria (rat), 445 and other cryoprotectants, in combination with RPS-2 or HP-5NP vehicle solutions, comparative effects on cultured vascular endothelial cells (pig), 413 in solutions and tissues, quantitation by HPLC (porcine myocardium), 210 DSC, see Differential scanning calorimetry E
Egg yolk in freezing extender, effect on spermatozoal motility in semen frozen and unfrozen in absence of glycerol (sheep), 43 Electron microscopy scanning, in study of structural states of freeze-dried and freeze-concentrated phosphates, 364 Embryo intracellular ice formation, subfreezing volumetric behavior and stochastic modeling (Drosophila melanogaster), 72 Endothelial cells vascular, in vitro effects of cryoprotectants in combination with vehicle solutions (pig), 413 Endothelium comeal, effects of equimolar combination of pro pane-l ,2-diol and glycerol (rabbit), 314 Erythrocytes dilution-associated swelling after freeze-thawing, prevention by sorbitol (human), 237
SUBJECT
suspended in sodium chloride solutions, freeze-thaw injury, effect of initial tonicity (human), 18 Ethanediol and other cryoprotectants, in combination with BPS-2 or HP-SNP vehicle solutions, comparative effects on cultured vascular endothelial cells (pig), 413 Ethylene glycol effects on low temperature gel structure of denatured collagen, 335 Eye, see specific parts F
Fasting effects on quality of liver preservation methods (rabbit), 227 Fertility effects of glycerol in frozen and unfrozen semen (sheep), 36 Fertilization vitrified oocytes in vitro, subsequent development, effects of dilution methods (mouse), 50 Fibroblasts V79 (Chinese hamster lung), survival after exposure to hypothermia, effects of Hepes, free radicals, and H,O, under light and dark conditions, 8 Fluorescent light and dark conditions, effects on survival of hypothermia-exposed V79 cells, 8 Free radicals effects on survival of hypothermia-exposed V79 cells, 8 Freeze-drying phosphate structural states after, SEM and DSC studies, 364 and storage, Escherichia co/i HBlOl with recombinant plasmid, effects on viability and genetic stability, 251 Freeze-thawing cycles, stability of phosphatidylcholine sonicated vesicles during, effect of Ca*+, 279 erythrocytes injury to cells suspended in sodium chloride solutions, effect of initial tonicity (human), 18 subsequent cell swelling after dilution, prevention by sorbitol (human), 237 phosphatidylcholine vesicles cryoprotection by trehalose, effect of Ca2+ , 347 cryoprotective effects of carbohydrate derivatives, 327 Freezing concentrated phosphates, structural states, SEM and DSC studies, 364 foot muscle, analysis of associated damage (Melampus bidentatus), 491 heart at high subzero temperatures for preservation (m), 171
607
INDEX
low-temperature, and storage, Escherichiu coli HBlOl with recombinant plasmid, effects on viability and genetic stability, 251 oocytes without cryoadditives, intracelhdar ice formation during, cryomicroscopic analysis, 55 orange trees, effects on main stem wood, 382 plate-shaped container for, specific location in sample for determination of cooling rate, computer simulations, 115 semen, development of extender: effects of osmotic pressure, egg yolk levels, sugar types, and method of dilution (sheep), 43 slow, associated mechanical stress due to extracellular ice crystal formation, induction of lamellar to hexagonal II phase transitions in hyphal tonoplasts (mushroom), 191 yeast, enhanced survival in cells expressing antifreeze gene analog, 474 Frog isolated spinal cord, seizures evoked by sudden cooling, role of excitatory amino acids, 255 Fungi mushroom, hyphal tonoplasts, lamellar to hexagonal II phase transitions, induction by mechanical stress due to extracellular ice crystal formation, 191 Saccharomyces cerevisiae, expressing antifreeze gene analog, enhanced survival after freezing, 474 G
Galactose cryoprotective effects on phosphatidylcholine vesicles, 327 Gels denatured collagen, structure at low temperature, effects of cryosolvents, 335 Genes antifreeze, analog, yeast expressing, enhancement of survival after freezing, 474 Genetic stability and viability, Escherichiu coli HBlOl with recombinant plasmid, effects of preservation methods, 251 Glass transitions and relaxation, in vitrified glycerol solutions, DSC study, 87 various, solidified aqueous solutions of glycerol, analysis, 268 Glucose in preservation solution, effect on cold-stored liver (rat), 428 Glycerol aqueous solutions, glassy state, DSC study, 87 effect on functional recovery of isolated heart after freezing preservation at high subzero temperatures (rat), 171
SUBJECT INDEX
608
in equimolar combination with propane-l ,Zdiol, effects on cornea (rabbit), 314 in frozen and unfrozen semen, effects on fertility rates (sheep), 36 and other cryoprotectants, in combination with RPS-2 or HP-SNP vehicle solutions, comparative effects on cultured vascular endothelial cells (pig), 413 solidified aqueous solutions, cryobehavior analysis, 268 Glycine prevention of cold ischemic injury in isolated hepatocytes (rat), 105 Ground squirrel cardiac sarcoplasmic reticulum calcium uptake and accumulation, effects of hibernation, 354 H
Heart glutathione depletion in cold storage (rabbit), 143 isolated, freezing preservation at high subzero temperatures (rat), 171 right ventricle, papillary muscle, sodium and calcium currents, temperature effects (hedgehog, rat), 96 sarcoplasmic reticulum, calcium uptake and accumulation, effects of hibernation (ground squirrel), 354 Hedgehog ventricular muscle, sodium and calcium currents, temperature effects, 96 Hepatocytes, see also Liver cold ischemic injury, prevention by glycine (rat), 105 cold-stored, lipid peroxidation, effect of polyethylene glycol (rat), 1 intracelhdar ice formation, characterization by cryomicroscopy (rat), 436 Hibernation effects on calcium uptake and accumulation in cardiac sarcoplasmic reticulum (ground squirrel), 354 High-performance liquid chromatography in quantitation of dimethyl sulfoxide in solutions and tissues (porcine myocardium), 210 Hindpaw microvascular patency after cooling to heat of fusion and subsequent rapid rewarming, evaluation by corrosion casting (rat), 294 HP-SNP and RPS-2, in combination with four cryoprotectants, comparative effects on cultured vascular endothelial cells (pig), 413 Human erythrocytes dilution-associated swelling after freeze-thawing, prevention by sorbitol, 237
suspended in sodium chloride solutions, freezethaw injury, effect of initial tonicity, 18 liver slices biotransformation in, effects of vitrification, 216 cryopreservation, 131 monocytes, membrane osmotic properties at subfreezing temperatures, cryomicroscopic analysis, 391 semen cryopreservation, cryosurvival of Trichomonas vaginalis during, evaluation, 246 Hydrogen peroxide effects on survival of hypothermia-exposed V79 cells, 8 Hypothermia exposed V79 cells, survival, effects of Hepes, free radicals, and H,O, under light and dark conditions, 8 induced cell swelling, suppression in cold-stored liver, functional effects (rat), 150 intrinsic alteration of arterial reactive properties (rabbit), 499
Ice extracellular formation crystals, resulting mechanical stress, induction of lamellar to hexagonal II phase transitions in hyphal tonoplasts (mushroom), 191 in foot muscle, associated damage, analysis (Melampus bidentatus), 491 intracellular formation in embryos: subfreezing volumetric behavior and stochastic modeling (Drosophila melanogaster), 72 in hepatocytes, characterization by cryomicroscopy (rat), 436 in oocytes during freezing without cryoadditives, cryomicroscopic analysis (mouse), 55 nucleation agents, tissue distribution in larvae (rice stem borer), 376 Inflammation pawskin, acute response to cold injury, inhibition by corticotropin-releasing factor (rat), 185 Injury cold, see Cold injury cold ischemic, in isolated hepatocytes, prevention by glycine (rat), 105 freeze-thawing, erythrocytes suspended in sodium chloride solutions, effect of initial tonicity (human), 18 Insects fruitfly (Drosophila melanogaster), embryos, intracellular ice formation: subfreezing volumetric behavior and stochastic modeling, 72 rice stem borer (Chile suppressalis), larvae, tissue distribution of ice-nucleating agents, 376
SUBJECT
Ischemia cold, induced injury in isolated hepatocytes, prevention by glycine (rat), 105 K Kidney glutathione depletion in cold storage (rabbit), 143 L Larvae tissue distribution of ice-nucleating agents (rice stem borer), 376 L-33 tissue membrane phospholipids, nonfreezing cold injury-associated degradation, protection by quinacrine (rabbit), 177 Light fluorescent, see Fluorescent light Lipid peroxidation in cold-stored hepatocytes, effect of polyethylene glYCO1(rat), 1 Liposomes cryoprotective mechanism of dimethyl sulfoxide, 467 Liver, see also Hepatocytes cold-stored, effects of sugars in preservation solution (rat), 428 isolated perfused, preservation, effects of donor pretreatment with chlorpromazine, quinacrine, and verapamil (rat), 422 mitochondria, protein synthesis and membrane transport, effects of cryopreservation (rat), 445 preservation by various methods, effects of fasting (rabbit), 227 preserved by cold storage, functional effects of sup pression of hypothermia-induced cell swelling (rat), 150 slices biotransformation in, effects of vitrification (human), 216 cryopreservation (human, pig), 131 tissue and cellular glutathione depletion in cold storage (rabbit, rat), 143 Lymphocytes fresh and cryopreserved, in vitro reactivity, comparison (pig), 405
INDEX
609
phospholipid bilayer, cryoprotective mechanism of dimethyl sulfoxide, 467 Membrane transport in hepatic mitochondria, effect of cryopreservation (rat), 445 Methanol effects on low temperature gel structure of denatured collagen, 335 Mitochondria hepatic, protein synthesis and membrane transport, effects of cryopreservation (rat), 445 Models mathematical, heat transfer: optimal location of thermal sensor, 115 stochastic, intracellular ice formation in embryos (Drosophila melanogaster), 72 Monocytes membrane osmotic properties at subfreezing temperatures, cryomicroscopic analysis (human), 391 Motility spermatozoa in semen frozen and unfrozen in absence of glycerol, effects of dilution methods, osmolality, egg yolk levels, and sugar types (sheep), 43 Mouse oocytes intracellular ice formation during freezing without cryoadditives, cryomicroscopic analysis, 55 vitrified, development after in vitro fertilization, effects of dilution methods, 50 Muscle foot, ice formation and freezing damage (Melampus bidentatus), 491 ventricular, sodium and calcium currents, temperature effects (hedgehog, rat), % N Nerves
sciatic, autotomy and decrease in spinal substance P after cryolesioning (rat), 460 Norepinephrine arterial responsiveness, effects of hypothermia (rabbit), 499 0
Maltose cryoprotective effects on phosphatidylcholine vesicles, 327 Membranes monocyte, osmotic properties at subfreezing temperatures, cryomicroscopic analysis (human), 391 phosphatidylcholine vesicle, cryoprotective effects of carbohydrate derivatives, 327
Obituaries Ronald I. N. Greaves, 307 Jacob Levitt, 111 Vernon P. Perry, 207 Oocytes intracellular ice formation during freezing without cryoadditives, cryomicroscopic analysis (mouse), 55 vitrified, development after in vitro fertilization, effects of dilution methods (mouse), 50
SUBJECT INDEX
610
Osmolality freezing extender, effect on spermatozoal motility in semen frozen and unfrozen in absence of glycerol (sheep), 43 ovary thecal gland, seasonal changes (ground squirrel), 503 P
Perfusion continuous, liver, effects of fasting (rabbit), 227 Permeability osmotic, monocyte membranes at subfreezing temperatures, cryomicroscopic analysis (human), 391 Phase transitions lamellar to hexagonal II, in hyphal tonoplasts, induction by mechanical stress due to extracellular ice crystal formation (mushroom), 191 Phosphates freeze-concentrated and freeze-dried, structural states, SEM and DSC studies, 364 Phosphatidylcholine vesicles cryoprotection by carbohydrate derivatives, 327 trehalose, effect of Ca”, 347 sonicated, stability during freeze-thaw cycles, effect of Ca2+, 279 Phospholipids bilayers, cryoprotective mechanism of dimethyl sulfoxide, 467 membrane, nonfreezing cold injury-associated degradation, protection by quinacrine (rabbit leg), 177 pig liver slices, cryopreservation, 131 lymphocytes, fresh and cryopreserved, in vitro reactivity, comparison, 405 myocardium, dimethyl sulfoxide levels, HPLC assay, 210 sperm, cryopreserved, viability assessment by thermal stress test, 454 vascular endothelial cells in vitro, effects of cryoprotectants in combination with vehicle solutions, 413 Plants orange (Citrus sinensis), main stem wood, changes during supercooling and freezing, 382 pea (Pisum sativum), cryopreserved shoot tips, survival, effects of number of days in cryoprotectant-supplemented culture medium and terminal freezing temperature, 288 Plasmids recombinant, in Escherichia coli HBIOl, viability and genetic stability, effects of preservation methods, 251
Polyethylene glycol effect on lipid peroxidation in cold-stored hepatocytes (rat), 1 Preservation cryo-, see Cryopreservation liver, cold-stored, effects of donor pretreatment with chlorpromazine, quinacrine, and verapamil (rat), 422 Preservation solutions compositional changes, suppression of hypothermia-induced cell swelling in cold-stored liver (rat), 150 sugars in, effects on cold-stored liver (rat), 428 Propane-l ,2-diol, see Propylene glycol Propylene glycol in equimolar combination with glycerol, effects on cornea (rabbit), 314 and other cryoprotectants, in combination with RPS-2 or HP-5NP vehicle solutions, comparative effects on cultured vascular endothelial cells (pig), 413 vitrifiable concentrations, cornea1 tolerance (rabbit), 159; erratum, 509 in vitrification solution, effect on corneas (rabbit), Vol. 27, p. 42; erratum, 509 Proteins antifreeze, encoding gene analog, yeast expressing, enhanced survival after freezing, 474 ice nucleator, purification and partial characterization (Melampus bidentarus), 483 synthesis in hepatic mitochondria, effect of cryopreservation (rat), 445 Protozoa Trichomonas vaginalis, cryosurvival during cryopreservation of semen, evaluation (human), 246 Q Quinacrine effect as donor pretreatment in liver preservation (rat), 422 protection of membrane phospholipids from nonfreezing cold injury-associated degradation (rabbit leg), 177 R
Rabbit cornea effects of propane-1,2-diol in vitrification solution, Vol. 27, p. 42; erratum, 509 endothelium, effects of equimolar combination of propane-l ,2-dial and glycerol, 3 14 tolerance to vitrifiable concentrations of propanel,Zdiol, 159; erratum, 509 femoral artery, reactive properties, intrinsic alteration during hypothermia, 499 leg tissue, nonfreezing cold injury, protection by quinacrine, 177
SUBJECT
liver, quality of preservation methods, effects of fasting, 227 tissue glutathione, effects of cold storage, 143 Raffinose in preservation solution, effect on cold-stored liver (rat), 428 Rat heart, isolated, freezing preservation at high subzero temperatures, 171 hepatocytes cold ischemic injury, prevention by glycine, 105 cold-stored, lipid peroxidation, effect of polyethylene glycol, 1 glutathione depletion in cold storage, analysis, 143 intracellular ice formation, characterization by cryomicroscopy, 436 hindpaw, microvascular patency after cooling to heat of fusion and subsequent rapid rewarming, evaluation by corrosion casting, 294 liver cold-stored effects of sugars in preservation solution, 428 hypothermia-induced cell swelling, suppression: functional effects, 150 isolated perfused, preservation, effects of donor pretreatment with quinacrine, chlorpromazine, and verapamil, 422 mitochondria, hepatic, protein synthesis and membrane transport in, effects of cryopreservation, 445 pawskin, acute inflammatory response to cold injury, inhibition by corticotropin-releasing factor, 185 sciatic nerve cryolesion, subsequent autotomy and decrease in spinal substance P, 460 thyrotropin and thyroid hormones, effects of exposure to chronic-intermittent cold, 400 ventricular muscle, sodium and calcium currents, temperature effects, 96 Rewarming rate rapid, after cooling to heat of fusion, effects on microvascular corrosion casts (rat hindpaw), 294 RPS-2 and HP-SNP, in combination with four cryoprotectants, comparative effects on cultured vascular endothelial cells (pig), 413 S Sarcoplasmic reticulum cardiac, calcium uptake and accumulation, effects of hibernation (ground squirrel), 354 Seasonal change in ovarian thecal gland (ground squirrel), 503 Seizures spinal cord, evoked by sudden cooling, role of excitatory amino acids (frog, toad), 255
INDEX
611
Semen cryopreservation. cryosurvival of Trichomonas vagin& during, evaluation (human), 246 frozen and unfrozen in absence of glycerol: effects of osmolahty, egg yolk levels, sugar types, and method of dilution (sheep), 43 in presence or absence of glycerol, analysis: fertility trials and effects of dilution methods (sheep), 36 Sheep semen, frozen and unfrozen in absence of glycerol: effects of osmolahty, egg yolk levels, sugar types, and method of dilution, 43 in presence or absence of glycerol, analysis: fertility trials and effects of dilution methods, 36 Shoot tips cryopreserved, survival, effects of number of days in cryoprotectant-supplemented culture medium and terminal freezing temperature (pea), 288 Skin paw, acute inflammatory response to cold injury, inhibition by corticotropin-releasing factor (rat), 185 Snail foot muscle, ice formation and freezing damage, 491 ice nucleator protein, purification and partial characterization, 483 Sodium chloride solutions, erythrocytes suspended in, freeze-thaw injury, effect of initial tonicity (human), 18 Sodium currents and calcium currents, in ventricular muscle, temperature effects (hedgehog, rat), % Solutions aqueous glycerol, solidified, cryobehavior analysis, 268 vitrified glycerol, DSC study, 87 cryopreservative, dimethyl sulfoxide in, quantitation by HPLC, 210 preservation, see Preservation solutions sodium chloride, erythrocytes suspended in, freezethaw injury, effect of initial tonicity (human), 18 vehicle, in combination with cryoprotectants, comparative effects on cultured vascular endothelial cells (pig), 413 vitritication, see Vitrification solutions Sorbitol prevention of dilution-associated cell swelling after freeze-thawing (human erythrocytes), 237 Spermatozoa cryopreserved, viability assessment by thermal stress test (pig), 454 Spinal cord isolated, seizures evoked by sudden cooling, role of excitatory amino acids (frog, toad), 255
612
SUBJECT INDEX
substance P content, decrease after peripheral cryogenic nerve lesion (rat), 460 Squirrel ovarian thecal gland, seasonal changes, 503 Starvation effect on crystallization temperature of larval tissues (rice stem borer), 376 Storage cold, see Cold storage long-term, Escherichia coli HBlOl with recombinant plasmid preserved by different methods, effects on viability and genetic stability, 251 Stress mechanical, due to extracellular ice crystal formation, induction of lamellar to hexagonal II phase transitions in hyphal tonoplasts (mushroom), 191 Stress test thermal, in assessmet of viability of cryopreserved sperm (Pig), 454 Structural states freeze-concentrated and freeze-dried phosphates, SEM and DSC studies, 364 Substance P spinal content, decrease after peripheral cryogenic nerve lesion (rat), 460 Sucrose in preservation solution, effect on cold-stored liver (rat), 428 sugars various types in freezing extender, effect on spermatozoal motility in semen frozen and unfrozen in absence of glycerol (sheep), 43 Supercooling effects on main stem wood (orange), 382 Survival pea shoot tips cryopreserved in cryoprotectantsupplemented medium, effects of days of culture and terminal freezing temperature, 288 spermatozoa in semen frozen in absence of glycerol, effects of dilution methods (sheep), 36 Trichomonas vaginalis in semen during cryopreservation, evaluation (human), 246 V79 cells after exposure to hypothermia, effects of Hepes, free radicals, and H,O, under light and dark conditions, 8 yeast expressing antifreeze gene analog, enhancement after freezing, 474
T Temperature annealing, solidified aqueous solutions of glycerol, analysis, 268 effects on sodium and calcium currents in ventricular muscle (hedgehog, rat), 96
low chronic-intermittent exposure, effects on thyrotropin and thyroid hormones (rat), 400 denatured collagen gel structure at, effects of cryosolvents, 335 subfreezing, monocyte membrane osmotic properties at, cryomicroscopic analysis (human), 391 terminal freezing, effect on survival of pea shoot tips cryopreserved in cryoprotectant-supplemented medium, 288 Thecal gland ovarian, seasonal changes (ground squirrel), 503 Thyrotropin effects of chronic-intermittent cold (rat), 400 Thyroxine effects of chronic-intermittent cold (rat), 400 Tissues dimethyl sulfoxide levels, HPLC assay, 210 distribution of ice-nucleating agents in larvae (rice stem borer), 376 Toad isolated spinal cord, seizures evoked by sudden cooling, role of excitatory amino acids, 255 Tonicity initial, sodium chloride solutions, effect on freezethaw injury to suspended erythrocytes (human), 18 Tonoplasts hyphal, lamellar to hexagonal II phase transitions, induction by mechanical stress due to extracellular ice crystal formation (mushroom), 191 Trehalose cryoprotection of phosphatidylcholine vesicles, effect of Ca*+ , 347 Triiodothyronine effects of chronic-intermittent cold (rat), 400 V
Verapamil effect as donor pretreatment in liver preservation (rat), 422 Vesicles phosphatidylcholine, see Phosphatidylcholine vesicles Viability cryopreserved sperm, assessment by thermal stress test (pi), 454 and genetic stability, Escherichia coli HBlOl with recombinant plasmid, effects of preservation methods, 251 Vitrification glycerol solutions, glass transition and relaxation, DSC study, 87 liver slices, effects on biotransformation activity (human), 216 oocytes cryopreserved by, development after in
SUBJECT
virro fertilization, effects of dilution methods (mouse), 50 Vitrification solutions propane- 1,Zdiol effect on cornea (rabbit), Vol. 27, p. 42; erratum, 509 equimolar combination with glycerol, effects on cornea (rabbit), 314 in various concentrations, comeal tolerance (rabbit), 159; erratum, 509
613
INDEX
Volumetric behavior subfreezing, during intracellular ice formation in embryos, stochastic modeling (Drosophila melanogaster), 72 W wood
main stem, changes during supercooling and freezing (orange), 382