- Email: [email protected]
Substitution of the gene LolCDE from Pseudomonas aeruginosa into Escherichia coli
Abstracts / Journal of Biotechnology 231S (2016) S4–S109
Capitalizing agrotourist potential of the mountain region from Romania Tabita Cornelia Adamov, Dorinel Dragoi ∗ , Tiberiu Iancu, Andrea Feher Department of Management and Rural Development, Faculty of Agricultural Management, Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” from Timisoara, Romania E-mail address: [email protected] (D. Dragoi). The theme proposed, mountain agrotourism signs in the concerns taken at national and European level regarding the identification of some viable solutions that additionally leads to the development of mountain areas, both in terms of economic growth and also of employment. Romanian mountain areas offer many opportunities in terms of practicing rural tourism, in general and of agrotourism and ecotourism, in particular. The development of agrotourist activities in mountain areas constitutes a real opportunity for economic growth of Romanian mountain villages, highlighting the natural and cultural potential of Romanian rural areas. Promoting and encouraging agrotourism’s development causes besides, direct economic and social benefits, activities of agricultural biodiversity protection, favoring also the adaptation of agricultural productions and food obtained in peasant households from mountain areas at the high demand, existent on the market for the traditional Romanian products. Encouraging agrotourist activities in mountain areas should start, firstly, from the awareness of the available potential of these areas and from the necessity of implementing the sustainable rural development programs which allow the removal of the gaps between the mountains disadvantaged areas and those economically developed. National policy regarding the development of the mountain areas should target: - Economic development and increasing farmers’ competitiveness; - Increasing the attractiveness degree of mountain tourist areas and reducing the depopulation of these areas; - Environmental protection and biodiversity conservation of mountain areas; - Conservation and capitalizing on cultural and folklore resources. http://dx.doi.org/10.1016/j.jbiotec.2016.05.354 Romanian imports evolution of fish and fish products according to quality classes Ioana Mihaela Balan 1 , Sabin S. Chis 1,∗ , Simona Cristina Constantinescu 1 , Ramona Mariana Ciolac 1 , Oana Maria Sicoe-Murg 1 , Sabin Chis 2 1
Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” from Timisoara, Romania 2 “Aurel Vlaicu” University, Arad, Romania E-mail address: [email protected] (S.S. Chis). Romania is a country where fish consumption is constantly present in the population diet. Trade in fish and fish products on the internal market is achieved through fisheries, industrial factories, wholesalers and retailers importers, depending on the quality classes.
S101
Fish as a raw material, it is processed both frozen and chilled form. Also, in the last 4–5 years, at the level the aquaculture farms are found interest in creating conditioning and processing of primary production before commercialization. Evolution of imports of fish and fishery products in Romania in the past five years have seen an upward trend, registering an increase in the value of imported products in 2013 compared to 2009 of 11% and an increase of 16% compared to 2011. In 2014 Romania imported mainly frozen fish excluding fillets and other fish meat in Spain and 11,542 tonnes (13.695 million euros) and in the Netherlands 10,058 tonnes (11.164 million euros). The most important export quantities and values that were obtained by purchasing frozen fish (41% of the total imports), frozen mackerel owns the largest share, especially in the years 2012 and 2013. This is explained by market research showing that the mackerel is the first place in consumer preference. http://dx.doi.org/10.1016/j.jbiotec.2016.05.355 Substitution of the gene LolCDE from Pseudomonas aeruginosa into Escherichia coli Evelina Turnova 1,∗ , Zuzana Firdova 1 , Thomas Dougherty 2 , Marcela Bielikova 1 , Stephen Lory 2 , Andrej Dudas 1 1
Department of Molecular Biology, Faculty of Natural Sciences, Comenius University, Mlynska dolina, 842 15 Bratislava, Slovak Republic 2 Department of Microbiology and Immunobiology, Harvard Medical School, 77 Avenue Louis Pasteur, 021 15 Boston, MA, USA E-mail address: [email protected] (E. Turnova). Multiply drug resistant bacteria pose a serious challenge in clinical medicine. Currently, the options for treatment of serious Gram negative organism infections are narrowing. One of the challenging aspects of Gram negative antibiotic therapy is the presence of two dissimilar membranes, the cytoplasmic and outer membranes. The lipoprotein transport pathway has five protein components: The LolCDE complex that has ATPase activity, is essential and resides in the cytoplasmic membrane. The LolCDE complex of Escherichia coli has been shown to be consist of one copy each of the membrane spanning subunits LolC and LolE, and two copies of the ATPase subunit LolD. The disclosure of a small molecule inhibitor of the Lol pathway in E. coli with a pyrazole core was reported. This compound was shown to inhibit Lol transport by demonstrating the blocking of release of lipoprotein from E. coli spheroplasts, and by compound resistance mutations that were isolated in both the LolC and LolE components. On the other hand, the compound did not inhibit this pathway in Pseudomonas aeruginosa. The aim of our study is to substitute the essential gene LolCDE from E. coli into P. aeruginosa, and delete the version on the chromosome. Moreover, we want to test if the P. aeruginosa cells with gene from E. coli will be viable and sensitive to the pyrazole core based Lol pathway inhibitor. http://dx.doi.org/10.1016/j.jbiotec.2016.05.356
Capitalizing agrotourist potential of the mountain region from Romania Tabita Cornelia Adamov, Dorinel Dragoi ∗ , Tiberiu Iancu, Andrea Feher Department of Management and Rural Development, Faculty of Agricultural Management, Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” from Timisoara, Romania E-mail address: [email protected] (D. Dragoi). The theme proposed, mountain agrotourism signs in the concerns taken at national and European level regarding the identification of some viable solutions that additionally leads to the development of mountain areas, both in terms of economic growth and also of employment. Romanian mountain areas offer many opportunities in terms of practicing rural tourism, in general and of agrotourism and ecotourism, in particular. The development of agrotourist activities in mountain areas constitutes a real opportunity for economic growth of Romanian mountain villages, highlighting the natural and cultural potential of Romanian rural areas. Promoting and encouraging agrotourism’s development causes besides, direct economic and social benefits, activities of agricultural biodiversity protection, favoring also the adaptation of agricultural productions and food obtained in peasant households from mountain areas at the high demand, existent on the market for the traditional Romanian products. Encouraging agrotourist activities in mountain areas should start, firstly, from the awareness of the available potential of these areas and from the necessity of implementing the sustainable rural development programs which allow the removal of the gaps between the mountains disadvantaged areas and those economically developed. National policy regarding the development of the mountain areas should target: - Economic development and increasing farmers’ competitiveness; - Increasing the attractiveness degree of mountain tourist areas and reducing the depopulation of these areas; - Environmental protection and biodiversity conservation of mountain areas; - Conservation and capitalizing on cultural and folklore resources. http://dx.doi.org/10.1016/j.jbiotec.2016.05.354 Romanian imports evolution of fish and fish products according to quality classes Ioana Mihaela Balan 1 , Sabin S. Chis 1,∗ , Simona Cristina Constantinescu 1 , Ramona Mariana Ciolac 1 , Oana Maria Sicoe-Murg 1 , Sabin Chis 2 1
Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” from Timisoara, Romania 2 “Aurel Vlaicu” University, Arad, Romania E-mail address: [email protected] (S.S. Chis). Romania is a country where fish consumption is constantly present in the population diet. Trade in fish and fish products on the internal market is achieved through fisheries, industrial factories, wholesalers and retailers importers, depending on the quality classes.
S101
Fish as a raw material, it is processed both frozen and chilled form. Also, in the last 4–5 years, at the level the aquaculture farms are found interest in creating conditioning and processing of primary production before commercialization. Evolution of imports of fish and fishery products in Romania in the past five years have seen an upward trend, registering an increase in the value of imported products in 2013 compared to 2009 of 11% and an increase of 16% compared to 2011. In 2014 Romania imported mainly frozen fish excluding fillets and other fish meat in Spain and 11,542 tonnes (13.695 million euros) and in the Netherlands 10,058 tonnes (11.164 million euros). The most important export quantities and values that were obtained by purchasing frozen fish (41% of the total imports), frozen mackerel owns the largest share, especially in the years 2012 and 2013. This is explained by market research showing that the mackerel is the first place in consumer preference. http://dx.doi.org/10.1016/j.jbiotec.2016.05.355 Substitution of the gene LolCDE from Pseudomonas aeruginosa into Escherichia coli Evelina Turnova 1,∗ , Zuzana Firdova 1 , Thomas Dougherty 2 , Marcela Bielikova 1 , Stephen Lory 2 , Andrej Dudas 1 1
Department of Molecular Biology, Faculty of Natural Sciences, Comenius University, Mlynska dolina, 842 15 Bratislava, Slovak Republic 2 Department of Microbiology and Immunobiology, Harvard Medical School, 77 Avenue Louis Pasteur, 021 15 Boston, MA, USA E-mail address: [email protected] (E. Turnova). Multiply drug resistant bacteria pose a serious challenge in clinical medicine. Currently, the options for treatment of serious Gram negative organism infections are narrowing. One of the challenging aspects of Gram negative antibiotic therapy is the presence of two dissimilar membranes, the cytoplasmic and outer membranes. The lipoprotein transport pathway has five protein components: The LolCDE complex that has ATPase activity, is essential and resides in the cytoplasmic membrane. The LolCDE complex of Escherichia coli has been shown to be consist of one copy each of the membrane spanning subunits LolC and LolE, and two copies of the ATPase subunit LolD. The disclosure of a small molecule inhibitor of the Lol pathway in E. coli with a pyrazole core was reported. This compound was shown to inhibit Lol transport by demonstrating the blocking of release of lipoprotein from E. coli spheroplasts, and by compound resistance mutations that were isolated in both the LolC and LolE components. On the other hand, the compound did not inhibit this pathway in Pseudomonas aeruginosa. The aim of our study is to substitute the essential gene LolCDE from E. coli into P. aeruginosa, and delete the version on the chromosome. Moreover, we want to test if the P. aeruginosa cells with gene from E. coli will be viable and sensitive to the pyrazole core based Lol pathway inhibitor. http://dx.doi.org/10.1016/j.jbiotec.2016.05.356