- Email: [email protected]
Supercritical fluid chromatography in natural product analysis
Abstracts / European Journal of Integrative Medicine 6 (2014) 686–745
related symptoms is in agreement with the use of R6 in Chinese medicine to nourish Yin. The effect of R6 was found to be different for the Yin deficiency subjects and the Qi deficiency subjects. Measuring personalized treatment effects is therefore an essential step in the development of personalized medicine. http://dx.doi.org/10.1016/j.eujim.2014.09.032 Supercritical fluid chromatography in natural product analysis Sonja Sturm, Judith Taibon, Hermann Stuppner ∗ Institute of Pharmacy/Pharmacognosy, University of Innsbruck, Center of Molecular Biosciences Innsbruck (CMBI), Innrain 80/82, 6020 Innsbruck, Austria E-mail address: [email protected] (H. Stuppner). When supercritical fluid chromatography was first developed more than 50 years ago, it was considered as a revolutionary separation technique. However, it never emerged from the shade of GC and HPLC and its reputation slowly ebbed over the years. Within the last decade, the revived interest of manufacturers and scientists for this complementary technique which combines some of the best features of LC as well as GC lead to tremendous improvement of the SFC instrumentation resulting in a stable analytical platform with many advantages over the existing chromatographic methods. SFC has been approved as versatile, cost-efficient, green, user friendly high resolution technique with multi-detector compatibility. It can be applied for the separation of complex mixtures instead of normal phase LC minimizing the use of toxic solvents or reversed phase LC without the need to convert samples to highly aqueous diluents, or in the case of hydrophobic thermo-labile or non-volatile compounds instead of GC without any derivatization. Whereas in the past SFC has mainly been used for the analytical or preparative separation of chiral compounds, it is getting more and more attention in the arena of achiral separation nowadays. Several recent reviews reported various applications in the field of drug discovery or food analysis with emphasis on exceeding lipophilic substance classes as lipids or fatty acids. However, reports on the successful separation of more polar natural compounds are rare up to now. In our laboratories we recently evaluated the applicability of SFC for qualitative and quantitative analysis of various classes of compounds with interesting biological activities; amongst them plant and fungus derived constituents, as cyclic peptides, triterpenoids, diterpenoids, alkaloids and many more. SFC might be the longed for technique to fill the gap between LC and GC allowing high-throughput, high-resolution but also cost-effective and environmental-friendly analysis for a tremendous expanded field of possible applications in natural product analysis. http://dx.doi.org/10.1016/j.eujim.2014.09.033
699
Novel analytical tools for quality control in Traditional Chinese Medicine Christian W. Huck Institute of Analytical Chemistry and Radiochemistry, CCB – Center for Chemistry and Biomedicine, Leopold-Franzens University, Innrain 80/82, 6020 Innsbruck, Austria E-mail address: [email protected]. Due to the amount of immunomodulatory, anti-inflammatory and neuroprotective agents in Traditional Chinese Medicine (TCM) novel analytical tools are permanently demanded to ensure at any time quality and safeness. Therefore, it is our aim to further develop vibrational spectroscopic methodologies allowing estimating the quality of a medicinal plant during breeding by near-infrared spectroscopy (NIRS), infrared (IR) imaging on one hand and mass spectroscopic imaging techniques on the other hand. The immunomodulatory, anti-inflammatory and neuroprotective potential of deriving extracts are optimised employing Aquasolv® -procedures next to conventional extraction methods. IR based metabolic profiling is carried out employing multivariate data analysis to monitor any changes in quality. For a more detailed, selective and sensitive insight miniaturised liquid chromatographic methods hyphenated to mass spectrometry (-LC–MS) via an electrospray (ESI) or matrix-free material enhanced laser desorption ionisation (mf-MELDI) interface are applied using designed and tailored stationary phases. These analytical tools are helpful to monitor the immunomodulatory, anti-inflammatory and neuroprotective effects and are also applicable in bioguided isolation approaches and cell based screening assays. As some potent ingredients might be available in only very low amounts special pre-concentration techniques, e.g. hollow monoliths, are introduced. In this presentation the establishment of a systematic analytical platform for the screening of wrong constituents while increasing the quality and safety is reported. http://dx.doi.org/10.1016/j.eujim.2014.09.034 Challenges and solutions for the quality control of natural health products Yuan-Chun Ma Canadian Phytopharmaceuticals Corp., Vancouver, BC, Canada E-mail address: [email protected]. Standardization and efficient quality control are the most important issues in the natural health products industry today and require world-wide attention, action, and resolution. After analyzing a number of commercial products including some of the most popular options currently in the market, it is evident that major quality control problems plague the industry. Such issues include: the incorrect labelling of plant species, the lack of detectable active compounds present, lowered concentrations, and extracts spiked with related compounds in a bid to meet the
related symptoms is in agreement with the use of R6 in Chinese medicine to nourish Yin. The effect of R6 was found to be different for the Yin deficiency subjects and the Qi deficiency subjects. Measuring personalized treatment effects is therefore an essential step in the development of personalized medicine. http://dx.doi.org/10.1016/j.eujim.2014.09.032 Supercritical fluid chromatography in natural product analysis Sonja Sturm, Judith Taibon, Hermann Stuppner ∗ Institute of Pharmacy/Pharmacognosy, University of Innsbruck, Center of Molecular Biosciences Innsbruck (CMBI), Innrain 80/82, 6020 Innsbruck, Austria E-mail address: [email protected] (H. Stuppner). When supercritical fluid chromatography was first developed more than 50 years ago, it was considered as a revolutionary separation technique. However, it never emerged from the shade of GC and HPLC and its reputation slowly ebbed over the years. Within the last decade, the revived interest of manufacturers and scientists for this complementary technique which combines some of the best features of LC as well as GC lead to tremendous improvement of the SFC instrumentation resulting in a stable analytical platform with many advantages over the existing chromatographic methods. SFC has been approved as versatile, cost-efficient, green, user friendly high resolution technique with multi-detector compatibility. It can be applied for the separation of complex mixtures instead of normal phase LC minimizing the use of toxic solvents or reversed phase LC without the need to convert samples to highly aqueous diluents, or in the case of hydrophobic thermo-labile or non-volatile compounds instead of GC without any derivatization. Whereas in the past SFC has mainly been used for the analytical or preparative separation of chiral compounds, it is getting more and more attention in the arena of achiral separation nowadays. Several recent reviews reported various applications in the field of drug discovery or food analysis with emphasis on exceeding lipophilic substance classes as lipids or fatty acids. However, reports on the successful separation of more polar natural compounds are rare up to now. In our laboratories we recently evaluated the applicability of SFC for qualitative and quantitative analysis of various classes of compounds with interesting biological activities; amongst them plant and fungus derived constituents, as cyclic peptides, triterpenoids, diterpenoids, alkaloids and many more. SFC might be the longed for technique to fill the gap between LC and GC allowing high-throughput, high-resolution but also cost-effective and environmental-friendly analysis for a tremendous expanded field of possible applications in natural product analysis. http://dx.doi.org/10.1016/j.eujim.2014.09.033
699
Novel analytical tools for quality control in Traditional Chinese Medicine Christian W. Huck Institute of Analytical Chemistry and Radiochemistry, CCB – Center for Chemistry and Biomedicine, Leopold-Franzens University, Innrain 80/82, 6020 Innsbruck, Austria E-mail address: [email protected]. Due to the amount of immunomodulatory, anti-inflammatory and neuroprotective agents in Traditional Chinese Medicine (TCM) novel analytical tools are permanently demanded to ensure at any time quality and safeness. Therefore, it is our aim to further develop vibrational spectroscopic methodologies allowing estimating the quality of a medicinal plant during breeding by near-infrared spectroscopy (NIRS), infrared (IR) imaging on one hand and mass spectroscopic imaging techniques on the other hand. The immunomodulatory, anti-inflammatory and neuroprotective potential of deriving extracts are optimised employing Aquasolv® -procedures next to conventional extraction methods. IR based metabolic profiling is carried out employing multivariate data analysis to monitor any changes in quality. For a more detailed, selective and sensitive insight miniaturised liquid chromatographic methods hyphenated to mass spectrometry (-LC–MS) via an electrospray (ESI) or matrix-free material enhanced laser desorption ionisation (mf-MELDI) interface are applied using designed and tailored stationary phases. These analytical tools are helpful to monitor the immunomodulatory, anti-inflammatory and neuroprotective effects and are also applicable in bioguided isolation approaches and cell based screening assays. As some potent ingredients might be available in only very low amounts special pre-concentration techniques, e.g. hollow monoliths, are introduced. In this presentation the establishment of a systematic analytical platform for the screening of wrong constituents while increasing the quality and safety is reported. http://dx.doi.org/10.1016/j.eujim.2014.09.034 Challenges and solutions for the quality control of natural health products Yuan-Chun Ma Canadian Phytopharmaceuticals Corp., Vancouver, BC, Canada E-mail address: [email protected]. Standardization and efficient quality control are the most important issues in the natural health products industry today and require world-wide attention, action, and resolution. After analyzing a number of commercial products including some of the most popular options currently in the market, it is evident that major quality control problems plague the industry. Such issues include: the incorrect labelling of plant species, the lack of detectable active compounds present, lowered concentrations, and extracts spiked with related compounds in a bid to meet the