Survival and pregnancy outcome after ultrarapid freezing of human embryos

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Citations from the Literature

and 44% per procedure in the FF group. We conclude that the use of FF for capacitation and as a gamete transfer medium significantly improves GIFT pregnancy rates and outcome. Survival and pregnancy of human embryos

outcome

after ultrarapid

freezing

Gordts S; Roziers P; Campo R; Noto V Medical Center for Fertility Diagnostics and In Vitro Fertilization and Embryo Transfer, Tiensevest 168, B-3000 Leuven, BEL FERTIL STERIL 1990,53/3 (469-472) Contrary to the belief that rapid cooling and thawing of mammalian embryos is detrimental to survival, it has been shown that under certain conditions mammalian embryos can survive rapid freezing-thawing. In this study, 237 human fertilized oocytes (93 pronucleates, 20 multipronucleates, and 124 cleaved embryos) were frozen for a period of 1 day to 7 months using the ultrarapid freezing system. After thawing, 94% of the embryos in the pronucleate group and 89.5% in the multipronucieate group showed normal morphological features, and 79% and 71%, respectively, started to cleave. Forty cleaved embryos were also frozen-thawed, but only 15 (37.5%) survived at thawing. Thirty-four frozen-thawed cleaved embryos were transferred to 20 patients during spontaneous cycles. Four patients became pregnant.

GYNECOLOGICAL ENDOCRINOLOGY Endocrine and behavioral responses to psychological stress in byderandrogenic women

Model1 E; Goldstein D; Reyes FI Division of Reproductive Endocrinology, Department of Obstetrics and Gynecology, Box 24, State University of New York Health Science Center, 450 Clarkson Avenue, Brooklyn, NY 11203, USA FERTIL STERIL 1990,53/3 (454-459) Stress has been implicated in the physiopathology of the ovarian androgenic syndrome. To explore further this notion, we compared the behavioral and endocrine responses to a mental stressor between women with hyperandrogenism (n = 13) and normals (n = 11). The standardized psychological stimulus produced higher levels of anxiety in the hyperandrogenic group than in controls. The endocrine (cortisol, prolactin, growth hormone, /I-endorphin) responses post stressor were definitely dissociated. Both groups showed a comparable anticipatory stress cortisol-secretion response. The cortisol release was greater following the mental stressor in the hyperandrogenie group than in the normals. Thus, hyperandrogenic women appear to have an abnormally affected pituitary-adrenal activation, which may play a role in the pituitary ovarian disruption characteristic of the ovarian androgenic syndrome. Clinical pharmacodynamics of urinary follicle-stimulating hormone and its application for pharmacokinetic simulation program

Mizunuma H; Takagi T; Honjyo S; Ibuki Y; Igarashi M

Int J Gynecol Obstet 34

Department of Obstetrics and Gynecology, Gunma University School of Medicine, Gunma 371, JPN FERTIL STERIL 1990,53/3 (440-445) To clarify the pharmacodynamics of urinary gonadotropins, same doses of Metrodin (Serono laboratories, Aubonne, Switzerland) or Pergonal (Serono, Rome, Italy) were injected intramuscularly into normal adult men in a crossover manner, and serum follicle stimulating hormone (FSH) and luteinizing hormone were measured by radioimmunoassay. Foilicie-stimulating hormone bioavailability parameters of Metrodin (150 IU), such as peak concentration (c(max)), the time when C(max) is observed, half-life, and area under concentration, were8.9 + 2.5 mIU/mL, 7.7 + 2.1 hours, 36.0 f 16.4 hours, and 258.6 + 47.9 mIU/mL x hour, respectively, and were not statistically different from those of Pergonal. On the other hand, by daily administration of Metrodin into women with isolate gonadotropin deficiency, serum FSH levels were elevated gradually, reached peak levels within 4 days, and maintained the same levels until the doses were increased. This pattern of FSH accumulation was parallel with the simulation pattern calculated using bioavailability parameters obtained from normal men, suggesting that exogenously administered FSH distributes into peripheral circulation in the manner of a one compartment model. Increasing the human menopausal gonadotropin dose- does the response really improve? Pantos C; Thornton SJ; Speirs AL; Johnston I

Department of Obstetrics and Gynaecology, University of Melbourne, 132 Grattan Street, Carlton, Vic3053, A US FERTIL STERIL 1990,53/3 (436-439) This study assesses the effects of attempts to optimize human menopausal gonadotropin (hMG) dosage in 271 patients who had at least two hyperstimulation cycles for in vitro fertilization or gamete intrafallopian transfer. In the first cycle, all patients received clomiphene citrate and hMG 150 IU/d. In the second cycle, the hMG dose was increased in 45% of patients to try to increase the egg yield. In spite of the increase, the population response was practically identical in both cycles. Median numbers of eggs retrieved (6 versus 6), no eggs retrieved (0.4% versus l%), only one or two eggs retrieved (10% versus lo%), and canceled cycles (10% versus 10.7%). This suggests that increasing the hMG dosage above 150 IU does not increase the number of e,ggs retrieved. A poor response may be due to inherent differences in follicular development that cannot be overcome by increases in hMG dosage. \ Can growth hormone increase, after cionidine administration, predict the dose of human menopausal hormone needed for induction of ovulation?

Menashe Y; Lunenfeld B; Pariente C; Frenkel Y; Mashiach S Department of Obstetrics and Gynecology, Chaim Sheba Medical Center, Tel Hashomer 52621, ISR FERTIL STERIL 1990,53/3 (432-435) Recent observations claimed that growth hormone (GH) administration increased the sensitivity of the ovary to gonado-