Survivin Expression in Canine Tumours with Sebaceous Differentiation

Survivin Expression in Canine Tumours with Sebaceous Differentiation

141:4, 2009 ESVP/ECVP Proceedings 2009 281 13 14 CONCURRENT UVEODERMATOLOGICAL (VOGTKOYANAGI-HARADA-LIKE) SYNDROME AND POLYMYOSITIS IN A JACK RUS...

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141:4, 2009

ESVP/ECVP Proceedings 2009

281

13

14

CONCURRENT UVEODERMATOLOGICAL (VOGTKOYANAGI-HARADA-LIKE) SYNDROME AND POLYMYOSITIS IN A JACK RUSSELL TERRIER K. Baiker, E. Scurrell, T. Wagner, D. Walker, L. SolanoGallego, B. Summers, B. Smyth and S. Scho¨niger The Royal Veterinary College, University of London, Hatfield, UK Introduction: We describe the concurrent presence of uveodermatological (Vogt-Koyanagi-Harada-like) syndrome and polymyositis in a dog. Materials and Methods: Histopathological examination was carried out on the right globe of a 1-year-old male Jack Russell terrier after a history of bilateral anterior uveitis and bilateral enucleation in 2006. Three years later, the dog was presented with skin irritation, progressive weight loss, dysphagia and stiff gait. The dog was humanely destroyed due to poor prognosis and was submitted for post-mortem examination. Results: The enucleated globe showed marked granulomatous panuveitis with numerous melanin-laden macrophages. The inflammation extended into extraocular muscles. At necropsy the dog had generalized muscle atrophy. Microscopical examination revealed mononuclear polymyositis affecting skeletal muscles of the rump, extremities and oesophagus. Skin and mucous membranes showed mononuclear lichenoid interface inflammation with intralesional melanomacrophages. Examined lymph nodes contained numerous plasma cells and melanomacrophages. Conclusions: To our knowledge this is the first report of concurrent uveodermatological syndrome and polymyositis in a dog. In this case, autoimmune polymyositis was suspected due to the absence of a detectable infectious cause and the widespread distribution of the muscle lesions. Epitope spreading is one possible cause for the development of two or more immune-mediated diseases in one patient.

CUTANEOUS ANGIOCENTRIC LYMPHOMA (LYMPHOMATOID GRANULOMATOSIS) IN A CAT B. Baum *, H.-M. Dauner y and M. Hewicker-Trautweinz *Vetmedlabor, Div. Idexx Laboratories, Ludwigsburg, ySmall Animal Practice, Sindelfingen, Hannover and zUniversity of Veterinary Medicine, Hannover, Germany Introduction: Lymphomatoid granulomatosis (LG) is an unusual round cell tumour with angiocentric extravascular growth behaviour. The tumour is well known in human medicine, but is rarely diagnosed in animals. It occurs most commonly in the lung, but cutaneous and renal involvement are observed. A report of LG in a cat with the pulmonary form represents the only previous report in this species. Materials and Methods: A 12-year-old female domestic short hair cat showed deep necroses of the facial skin. The cat was humanely destroyed. Results: Histological examination revealed round cell accumulations with angiotropism and extensive infiltration of the walls of large and medium-sized vessels. Immunohistochemical studies showed that cells infiltrating the walls of blood vessels labelled positively for CD3. Conclusions: The findings are consistent with lymphomatoid granulomatosis and represent the first report of cutaneous manifestation of the disease in a cat.

15 PATHOGENESIS OF HIGH AND LOW PATHOGENIC AVIAN INFLUENZA VIRUSES IN PARTRIDGES K. Bertran *, A. Chaves *, R. Dolz *, A. Ramis *,y, E. Pe´rez z, z U. Hoffle , R. Valle *, R. Rivas *, N. Busquets * and N. Majo´*,y *Animal Health Research Center (CReSA), yDepartment of Health and Animal Anatomy, Autonomous University of Barcelona, Barcelona and z IREC, Spain Introduction: In the present study, an experimental infection with HPAI and LPAI viruses was carried out in partridges to study clinical signs, gross and microscopical lesions, viral distribution in tissues and viral shedding. Materials and Methods: Partridges (Alectoris rufa) were infected intranasally with H7N1 (HPAI) and H7N9 (LPAI) viruses. Clinical signs were recorded and oropharyngeal and cloacal swabs and feather pulp samples were obtained from all birds each day. At 3, 5, 10 and 15 days post-infection (dpi), three birds from each infected group were killed. Gross lesions were evaluated and tissue samples were obtained for histopathological studies. Results: Partridges infected with HPA1 first showed clinical signs (ruffled feathers, inactivity) at 3 dpi and mortality started at 4 dpi. At 8 dpi, surviving birds showed severe nervous signs. Immunohistochemistry and real time polymerase chain reaction confirmed the presence of viral antigen in tissues and viral excretion, respectively. No clinical signs were observed in LPAI virus infected partridges, but viral shedding was detected in some birds. Conclusions: The present study demonstrates that HPAI viruses cause severe disease and mortality in partridges.

16 SURVIVIN EXPRESSION IN CANINE TUMOURS WITH SEBACEOUS DIFFERENTIATION L. Bongiovanni, D. Malatesta and L. Della Salda Department of Comparative Biomedical Science, University of Teramo, Teramo, Italy Introduction: Survivin is a member of the inhibitor of apoptosis protein (IAP) family and exhibits low expression in most normal adult tissues. Elevated expression in tissues during embryonic development and in the majority of cancers, as well as in human sebaceous tumours, indicates potential diagnostic utility. In normal adult human skin, survivin has been recently proposed as a marker for keratinocyte stem cells. Materials and Methods: Fifteen canine tumours with sebaceous differentiation (2 carcinomas, 9 adenomas, 1 epithelioma, 3 hyperplasia) and five specimens of normal canine skin were evaluated histologically and immunohistochemically. Full-length survivin (0.7 mg/ml, rabbit polyclonal antibody, NOVUS Biologicals) expression was correlated with p53 (1 in 400, rabbit polyclonal anti-human antibody, CM1, Novocastra) expression and mitotic index (MI). Results: Survivin-positive nuclei in normal sebaceous glands were few and scattered. Pre-neoplastic lesions and benign tumours showed several survivin-positive nuclei and, apart from one case, there was no evidence of p53 expression. Numerous survivin-positive nuclei occurred in carcinomas together with a higher mitotic index and large numbers of p53 positive nuclei. Conclusions: The present study revealed for the first time that in dogs, as in man, nuclear survivin expression is increased in sebaceous carcinomas compared with normal glands and benign lesions. The findings suggest a direct correlation with biological behaviour, consistent with the anti-apoptotic and proliferative functions of the molecule.