- Email: [email protected]
Sweat duct milia — Computer-generated 3-dimensional reconstruction anarysis of structure
JSID Abstracts/J. Dematol. Sci. 8 (1994) 54-88
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109
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INLKJCIION OF CYCLIC HAIR GROWTH IN NUDE MICE BY ALLOGENHC BONE MARROW TR4NSPLANXXlION PLUS THYMUS GRAFT Y OHNISI%INOUE and T. HORIO, Department of Dermatology, Kansai Mcdii University, Osaka, Japan.
EXPRESSION OF ANOROGEN RECEPTOR, TYPE I AND TYPE II 5 a -REDUCTASE IN HUIAN DERYAL PAPILLA CELLS
It is unknown why nude mice arc haiiless, which luck thymus and normal Tall ontogeny. To cxaminc whcthcr thcrc is a d&t mlatiomhip bctwcen thcsc two phamtypcs of an autcsomal ruxssivc single gcac nu, i.e. hairless and T&xagc bonnal@, BALB/&Jcl+u/nu (BALB/wu) mice wcrc lethally itmdiaa and transplanted with auogeneic bone marrow cells (BMT) witi$wihwt thymus grai? (THG) fium wild type CS?JXbSJ-+/+ (B6/J) mice. Maawqic hair growth was obscrwd in a group of [B6/JBMTtB6/J-THG], but not in a group of [BM-BMT]. ‘Ibc hair gmwtb in the forma group was cyclic at tic& 3 week intervals and rcpcatcd several times during the observation period up to 4 months. Our observation may indicate that T&age alls play a role to qulatc the hair cycle in nude mice.
.I. NAKANISHI’, S. ITAMP, K. ADACHI’ and 8. TAKAYASU2, ’ Aeachi Research Laboratories, ShlwJdn Resewoh Center. and * DepartrMnt
of Dermatology,
Oita Medical
Universi(%
Japan
WI invrrligatsd Ihe expression of wdrbDan rmxplor, lypa I and type II Sa-reductrre in Cultured human dmmbl paptlh cells (DPQ tram VW~OUI body tltet By pOly~IWrbta chlln reaCtiOn rmpllficrtion. Androgan receptor mRNAs wore detected in DPC from beard, prilluy hair and frontal scalp hair, whik tha exprrrion was weak in DPC from Occipital scalp hair. Qpa II 5a-raductasa vat expressed only in beard DPC. btd type I 6 ~1-reduc(ase was equally expressed in all types of DPC. Th sxprrsslons of the rrwptor and Iha enzymes wem not bffeccted by androgm tnrtment. There results indicate lhal DPC from beard, uiltary hair and frontal scalp hair am mdrogpn tar@ cells and ttm difference of sonsitlvity of hairs to androgan is partially controlled by Me cell-typo specific expression
01 5 a -reductats.
113
110 SWEAT DUCT MILlA - COMPUTER-GENERATED RECONSTRUCTION ANARYSIS OF STRUCTURE
3-DIMENSIONAL
ELEVATED PLASMA SOD LEVELS IN SYSTEMIC SCLEROSIS
-
A. MORITA and T. TSUJI, Department of Dermatology, Nagoya City University MedicalSchool. Nagoya,Japan Y. HONDA, Kumamoto Kumamoto,
K. EGAWA, T. ONO, Department University khool of Medicine. Japan
of Dermatolow.
The structures of d complete and an mromplete sweat duct nullurn were studled by three-dlmenslonal reconstructi~l (JDR) analyst based on sew11 sectlom stammg with hernatoxylmeosm and ~mmunohrstochem~cal stammg wth CEA and CA 50 antibodies. The mode of connectxa ot the eccrine duct wth the cysts was slmllar m the tu’o mlha; the eccrme duct penetrated into the cyst wall at the center ot its base, formed a circular path wthm the wall, and opened into the mner cavity. CA 50 was detected throushout the entxe cyst of the complete mihum. whk ,t was not detected at the most apx3.l portion ot the mcomplete millurn, where the cyst wall fused wth the overlying eplderrms. Our results demonstrated that an mcomplete mkhum LS the result of fusion between cells derived from an eccrme duct and those derived tram the surroundmg epldermls. whk the formatlo” of a complete mihum dose not mvolve fusion.
Although the etiology and pathogenesis of systemic sclerosis (SS) are essentially unknown, SS has teen designatedas a collagen vamlar disease. The pathophysiokgy of SS is understood on the basis of the following three concepts: injury of the vessel wall, disturbed immune response and defective control of connectivetissue metabolism. Several environmental agents that generate oxygen free radicals have been reported to be associated with SS. The following hypothesis was previously suggested:free radiils interact with liids, DNA, proteins, prokoglycans and collagenand damagecapillary walls through pemxidationof lipid componentsof the endothelial cell membrane. Thus, endothelial cell damage may precede fibrosis. Oxygen free radical in plasmaof SS, however. has not been estimated. In this study we analyzedplasma sufxoxide dkmutase (SOD) laveb and fcund that plasma SOD lavels in SS were significantly higher than those in cunfrols. The SS patients with hipher SOD levels had a prevalence of skin sclerosis. These data suggest that the elevated plasma SOD levels may reflect a high producrionof oxygen free radicalsin SS.
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114
LOCALlUTlON OF GR0IWll-l FACTORS IN HUMAN FETAL HAlR FOLLICLES IN THE SECONDTRIMESTER
Disaccharide analysis of glywwminoglycans produced by fiioblwts from the peripheral and central park of morphea in the thrwdimensional culture.
MAKIYAMA’, L.T.SMITl+, and KA.HOLSROO@. ‘Depaltmenfs of Siokgkal Sb’udure and Medklne/DemMtokgy. Unlvwsity of Washlngkn school Of Medicine, Seattle. WA, end zlklvenily of florida. Gainesville, FL, U.S.A.
Y. YOKOYAMA. 0. ISHIKAWA and Y. MIYACHI. Department of Dermatology. Gunma University School of Medidne, Maebashi, Japan
Certain growlh fac4orshave been mpottad to kfluence follkular development in animal. In human. immatura hair folliis dlfferenliate into the bulbous hair peg stage at 18 to 15 wsaks esumatadgaalatknaI rge (EGA), and afler 19 weeks EGA, they a#xoad~ matum morphology. Anllbodies that racognize EGF. TGF-a. PDGF A and B chains warn uwd to study human fetal hair follkles in this second trimester. Both wilh anti-EGF and an6TGF-a antibodies,weak to moderate Immunomadivny was seen in the inner mot sheath (IRS), the bulge, the inner petl of the outer roof sheath (ORS). hair medulla, cottax, and matrix csllr; Me sebaceousgfand axhlb4ladsbungar staining. Confocal laser scanning micmscopyrevwlad pundate staking for EGF and TGF-a in both cytoplasmk and nuclear compa&wnts of the lnMgec&Is. Using anti-PDGFA chain and 6 chain antitxniies. moderate immunoreadivify was detected in IRS, the sebaceousgtand, md the interkr cells of the bulge; the IRS and the bulge sho%d malnly diffuaa. cy3oplaamkslaking along together v&h punclate paitem. Themsuits kdkatal that soluble gmMh facto!~ indudlng EGF. TOFU, PDGFs may play a mla In the grwth mgulatiin of fatal hair follicles.
Diwccharida analysis of glycowminoglycans (GAGS) of dermai fiiroblasts from the peripheral and central pads of two patients with motphea was performed by HPLC after PMP labeling. Fiirobiasts supplemented with L-ascorbic acid 2-phosphate can render cells to the organization of the dermis-like structure in vitro. The total amount of GAG was almost the same in both peripheral and central parts. With respect to the GAG composition, ADi4S (dermatan sulfate) increased in both parts in comparison with that of fibroblasts from normal individuals. This compositional change was similar to the sclerotic skin of systemic sderoderma. In eddilkm. the ratios of ADMS / ADI-HA (hyaluronic add) in the peripheral parts were much higher than those of the central parts. This may reflect that the sclerotic tiange In the peripheral part Is in progress and in remisaion in the central part, In conclusion, cultured fiirobiasts from morphea can conserve the abnormal phenotype in this culture system.
13
109
112
INLKJCIION OF CYCLIC HAIR GROWTH IN NUDE MICE BY ALLOGENHC BONE MARROW TR4NSPLANXXlION PLUS THYMUS GRAFT Y OHNISI%INOUE and T. HORIO, Department of Dermatology, Kansai Mcdii University, Osaka, Japan.
EXPRESSION OF ANOROGEN RECEPTOR, TYPE I AND TYPE II 5 a -REDUCTASE IN HUIAN DERYAL PAPILLA CELLS
It is unknown why nude mice arc haiiless, which luck thymus and normal Tall ontogeny. To cxaminc whcthcr thcrc is a d&t mlatiomhip bctwcen thcsc two phamtypcs of an autcsomal ruxssivc single gcac nu, i.e. hairless and T&xagc bonnal@, BALB/&Jcl+u/nu (BALB/wu) mice wcrc lethally itmdiaa and transplanted with auogeneic bone marrow cells (BMT) witi$wihwt thymus grai? (THG) fium wild type CS?JXbSJ-+/+ (B6/J) mice. Maawqic hair growth was obscrwd in a group of [B6/JBMTtB6/J-THG], but not in a group of [BM-BMT]. ‘Ibc hair gmwtb in the forma group was cyclic at tic& 3 week intervals and rcpcatcd several times during the observation period up to 4 months. Our observation may indicate that T&age alls play a role to qulatc the hair cycle in nude mice.
.I. NAKANISHI’, S. ITAMP, K. ADACHI’ and 8. TAKAYASU2, ’ Aeachi Research Laboratories, ShlwJdn Resewoh Center. and * DepartrMnt
of Dermatology,
Oita Medical
Universi(%
Japan
WI invrrligatsd Ihe expression of wdrbDan rmxplor, lypa I and type II Sa-reductrre in Cultured human dmmbl paptlh cells (DPQ tram VW~OUI body tltet By pOly~IWrbta chlln reaCtiOn rmpllficrtion. Androgan receptor mRNAs wore detected in DPC from beard, prilluy hair and frontal scalp hair, whik tha exprrrion was weak in DPC from Occipital scalp hair. Qpa II 5a-raductasa vat expressed only in beard DPC. btd type I 6 ~1-reduc(ase was equally expressed in all types of DPC. Th sxprrsslons of the rrwptor and Iha enzymes wem not bffeccted by androgm tnrtment. There results indicate lhal DPC from beard, uiltary hair and frontal scalp hair am mdrogpn tar@ cells and ttm difference of sonsitlvity of hairs to androgan is partially controlled by Me cell-typo specific expression
01 5 a -reductats.
113
110 SWEAT DUCT MILlA - COMPUTER-GENERATED RECONSTRUCTION ANARYSIS OF STRUCTURE
3-DIMENSIONAL
ELEVATED PLASMA SOD LEVELS IN SYSTEMIC SCLEROSIS
-
A. MORITA and T. TSUJI, Department of Dermatology, Nagoya City University MedicalSchool. Nagoya,Japan Y. HONDA, Kumamoto Kumamoto,
K. EGAWA, T. ONO, Department University khool of Medicine. Japan
of Dermatolow.
The structures of d complete and an mromplete sweat duct nullurn were studled by three-dlmenslonal reconstructi~l (JDR) analyst based on sew11 sectlom stammg with hernatoxylmeosm and ~mmunohrstochem~cal stammg wth CEA and CA 50 antibodies. The mode of connectxa ot the eccrine duct wth the cysts was slmllar m the tu’o mlha; the eccrme duct penetrated into the cyst wall at the center ot its base, formed a circular path wthm the wall, and opened into the mner cavity. CA 50 was detected throushout the entxe cyst of the complete mihum. whk ,t was not detected at the most apx3.l portion ot the mcomplete millurn, where the cyst wall fused wth the overlying eplderrms. Our results demonstrated that an mcomplete mkhum LS the result of fusion between cells derived from an eccrme duct and those derived tram the surroundmg epldermls. whk the formatlo” of a complete mihum dose not mvolve fusion.
Although the etiology and pathogenesis of systemic sclerosis (SS) are essentially unknown, SS has teen designatedas a collagen vamlar disease. The pathophysiokgy of SS is understood on the basis of the following three concepts: injury of the vessel wall, disturbed immune response and defective control of connectivetissue metabolism. Several environmental agents that generate oxygen free radicals have been reported to be associated with SS. The following hypothesis was previously suggested:free radiils interact with liids, DNA, proteins, prokoglycans and collagenand damagecapillary walls through pemxidationof lipid componentsof the endothelial cell membrane. Thus, endothelial cell damage may precede fibrosis. Oxygen free radical in plasmaof SS, however. has not been estimated. In this study we analyzedplasma sufxoxide dkmutase (SOD) laveb and fcund that plasma SOD lavels in SS were significantly higher than those in cunfrols. The SS patients with hipher SOD levels had a prevalence of skin sclerosis. These data suggest that the elevated plasma SOD levels may reflect a high producrionof oxygen free radicalsin SS.
111
114
LOCALlUTlON OF GR0IWll-l FACTORS IN HUMAN FETAL HAlR FOLLICLES IN THE SECONDTRIMESTER
Disaccharide analysis of glywwminoglycans produced by fiioblwts from the peripheral and central park of morphea in the thrwdimensional culture.
MAKIYAMA’, L.T.SMITl+, and KA.HOLSROO@. ‘Depaltmenfs of Siokgkal Sb’udure and Medklne/DemMtokgy. Unlvwsity of Washlngkn school Of Medicine, Seattle. WA, end zlklvenily of florida. Gainesville, FL, U.S.A.
Y. YOKOYAMA. 0. ISHIKAWA and Y. MIYACHI. Department of Dermatology. Gunma University School of Medidne, Maebashi, Japan
Certain growlh fac4orshave been mpottad to kfluence follkular development in animal. In human. immatura hair folliis dlfferenliate into the bulbous hair peg stage at 18 to 15 wsaks esumatadgaalatknaI rge (EGA), and afler 19 weeks EGA, they a#xoad~ matum morphology. Anllbodies that racognize EGF. TGF-a. PDGF A and B chains warn uwd to study human fetal hair follkles in this second trimester. Both wilh anti-EGF and an6TGF-a antibodies,weak to moderate Immunomadivny was seen in the inner mot sheath (IRS), the bulge, the inner petl of the outer roof sheath (ORS). hair medulla, cottax, and matrix csllr; Me sebaceousgfand axhlb4ladsbungar staining. Confocal laser scanning micmscopyrevwlad pundate staking for EGF and TGF-a in both cytoplasmk and nuclear compa&wnts of the lnMgec&Is. Using anti-PDGFA chain and 6 chain antitxniies. moderate immunoreadivify was detected in IRS, the sebaceousgtand, md the interkr cells of the bulge; the IRS and the bulge sho%d malnly diffuaa. cy3oplaamkslaking along together v&h punclate paitem. Themsuits kdkatal that soluble gmMh facto!~ indudlng EGF. TOFU, PDGFs may play a mla In the grwth mgulatiin of fatal hair follicles.
Diwccharida analysis of glycowminoglycans (GAGS) of dermai fiiroblasts from the peripheral and central pads of two patients with motphea was performed by HPLC after PMP labeling. Fiirobiasts supplemented with L-ascorbic acid 2-phosphate can render cells to the organization of the dermis-like structure in vitro. The total amount of GAG was almost the same in both peripheral and central parts. With respect to the GAG composition, ADi4S (dermatan sulfate) increased in both parts in comparison with that of fibroblasts from normal individuals. This compositional change was similar to the sclerotic skin of systemic sderoderma. In eddilkm. the ratios of ADMS / ADI-HA (hyaluronic add) in the peripheral parts were much higher than those of the central parts. This may reflect that the sclerotic tiange In the peripheral part Is in progress and in remisaion in the central part, In conclusion, cultured fiirobiasts from morphea can conserve the abnormal phenotype in this culture system.