- Email: [email protected]
Sweden — Carbohydrate biosensor surface for uropathogenic bacteria detection
Biosensors & Bioelectronics Vol. 10 No. 5 (1995)
of Hope Hospital report on "Biosensors: A viable monitoring technology?". Biosensors for practical in vivo and in vitro applications are dependent on the effective integration of several biological and physical technologies. Some of the more recent advances aimed at taking techniques of fundamental and academic interest to various forms of practical reagentless biochemical analysis are highlighted, w i t h a s s o c i a t e d c l i n i c a l and c o m m e r c i a l consequences. The paper describes some of the most recent developments in biosensor research, in particular those relating to material aspects of fabrication, including multilayer films for sensor applications, advances in ISFETs, conjugated polymers, new developments in quartz crystal based biosensors, as well as a d v a n c e s in a m p e r o m e t r i c e n z y m e e l e c t r o d e s and the application of devices for continuous monitoring. Contact: Department of Medicine, Section of Clinical Biochemistry, University of Manchester, Hope Hospital, Eccles Old Rd, Salford M6 8HD, UK.
Japan - Interactions between collagen-binding stress protein HSP47 and collagen. Analysis of kinetic parameters by surface plasmon resonance biosensor In J. BIOL. CHEM. (269/49 (31224-31228) 1994), T. Natsume, T. Koide, S. -I. Yokota, K. Hirayoshi & K. Nagata, of Nippon Meat Packers Inc. r e p o r t on " I n t e r a c t i o n s b e t w e e n collagen-binding stress protein HSP47 and collagen. Analysis of kinetic parameters by surface plasmon resonance biosensor". In this report, the interaction of HSP47 and types I to V collagen were analyzed using BIAcore TM system, an optical biosensor based on the principles of surface plasmon resonance. Types I-V collagen were purified from porcine skin, porcine articular cartilage, bovine lens capsule, and porcine placenta and immobilized on sensorchips of the BIAcore TM system at a surface c o n c e n t r a t i o n o f 10-15 n g / m m 2. P u r i f i e d recombinant mouse HSP47 (rmHSP47) expressed in Escherichia coli was passed over the
AB~RACTS
sensorchips at a flow rate of 2 ~l/min and binding curves of rmHSP47 to collagens were monitored. Using this approach, accurate association and dissociation rate constants were determined in addition to dissociation constants, rmHSP47 was found to bind to types I-V collagen with similar dissociation constants of the order of 10 .7 M. This relatively low dissociation constant resulted from the ra~id dissociation rate constant (k(diss4) 10 -2 s- ) and considerably high (k(ass) 2 x 10 M -1 s-1) association rate constant. These kinetic parameters may reflect a transient interaction between HSP47 and procollagen in vivo. Contact: Research and Development Center, Nippon Meat Packers Inc., 3-3 Midorigahara, Tsukuba City, Ibaraki 300-26, Japan.
Sweden - Carbohydrate biosensor surface for uropathogenic bacteria detection In BIOTECHNOLOGY (12/13 (1376-1378) 1994) K.G.I. Nilsson & C.-F Mandenius of Glycorex AB report on "A carbohydrate biosensor surface for the detection of uropathogenic bacteria". The authors have developed a new surface for use in biosensors that is based on a gold plate covered with a specific carbohydrate receptor structure. The carbohydrate, GaloO-4Gal, was bound covalently via a thioalkylcarboxy- spacer, or a d s o r b e d as a n e o g l y c o p r o t e i n , to a two-dimensional gold surface. Both types of surfaces showed high specificity in the binding of the u r o p a t h o g e n i c b a c t e r i a P - f i m b r i a t e d Escherichia coli compared to the binding of non-infectious bacteria. The signal to noise ratio is sufficiently high to allow specific detection of the bacteria in biosensor applications. Contact: Glycorex AB, Solveg. 41, S-223 70 Lund, Sweden.
Sweden - Microbiosensor based on an integrated thermopile In ANAL. CHIM. ACTA (299/2 (165-170) 1994) B. Xie, M. Mecklenburg, B. Danielsson, O. Ohman & F. Winquist, of University of Lund
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of Hope Hospital report on "Biosensors: A viable monitoring technology?". Biosensors for practical in vivo and in vitro applications are dependent on the effective integration of several biological and physical technologies. Some of the more recent advances aimed at taking techniques of fundamental and academic interest to various forms of practical reagentless biochemical analysis are highlighted, w i t h a s s o c i a t e d c l i n i c a l and c o m m e r c i a l consequences. The paper describes some of the most recent developments in biosensor research, in particular those relating to material aspects of fabrication, including multilayer films for sensor applications, advances in ISFETs, conjugated polymers, new developments in quartz crystal based biosensors, as well as a d v a n c e s in a m p e r o m e t r i c e n z y m e e l e c t r o d e s and the application of devices for continuous monitoring. Contact: Department of Medicine, Section of Clinical Biochemistry, University of Manchester, Hope Hospital, Eccles Old Rd, Salford M6 8HD, UK.
Japan - Interactions between collagen-binding stress protein HSP47 and collagen. Analysis of kinetic parameters by surface plasmon resonance biosensor In J. BIOL. CHEM. (269/49 (31224-31228) 1994), T. Natsume, T. Koide, S. -I. Yokota, K. Hirayoshi & K. Nagata, of Nippon Meat Packers Inc. r e p o r t on " I n t e r a c t i o n s b e t w e e n collagen-binding stress protein HSP47 and collagen. Analysis of kinetic parameters by surface plasmon resonance biosensor". In this report, the interaction of HSP47 and types I to V collagen were analyzed using BIAcore TM system, an optical biosensor based on the principles of surface plasmon resonance. Types I-V collagen were purified from porcine skin, porcine articular cartilage, bovine lens capsule, and porcine placenta and immobilized on sensorchips of the BIAcore TM system at a surface c o n c e n t r a t i o n o f 10-15 n g / m m 2. P u r i f i e d recombinant mouse HSP47 (rmHSP47) expressed in Escherichia coli was passed over the
AB~RACTS
sensorchips at a flow rate of 2 ~l/min and binding curves of rmHSP47 to collagens were monitored. Using this approach, accurate association and dissociation rate constants were determined in addition to dissociation constants, rmHSP47 was found to bind to types I-V collagen with similar dissociation constants of the order of 10 .7 M. This relatively low dissociation constant resulted from the ra~id dissociation rate constant (k(diss4) 10 -2 s- ) and considerably high (k(ass) 2 x 10 M -1 s-1) association rate constant. These kinetic parameters may reflect a transient interaction between HSP47 and procollagen in vivo. Contact: Research and Development Center, Nippon Meat Packers Inc., 3-3 Midorigahara, Tsukuba City, Ibaraki 300-26, Japan.
Sweden - Carbohydrate biosensor surface for uropathogenic bacteria detection In BIOTECHNOLOGY (12/13 (1376-1378) 1994) K.G.I. Nilsson & C.-F Mandenius of Glycorex AB report on "A carbohydrate biosensor surface for the detection of uropathogenic bacteria". The authors have developed a new surface for use in biosensors that is based on a gold plate covered with a specific carbohydrate receptor structure. The carbohydrate, GaloO-4Gal, was bound covalently via a thioalkylcarboxy- spacer, or a d s o r b e d as a n e o g l y c o p r o t e i n , to a two-dimensional gold surface. Both types of surfaces showed high specificity in the binding of the u r o p a t h o g e n i c b a c t e r i a P - f i m b r i a t e d Escherichia coli compared to the binding of non-infectious bacteria. The signal to noise ratio is sufficiently high to allow specific detection of the bacteria in biosensor applications. Contact: Glycorex AB, Solveg. 41, S-223 70 Lund, Sweden.
Sweden - Microbiosensor based on an integrated thermopile In ANAL. CHIM. ACTA (299/2 (165-170) 1994) B. Xie, M. Mecklenburg, B. Danielsson, O. Ohman & F. Winquist, of University of Lund
iii