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adrb3
Oral Presentation
e163
Sympathetic nerves inhibit MSC migration and osteogenisis in distraction osteogenesis via norepinephrine/adrb3 L. Wang ∗ , Z. Du, J. Cao, D. Lei State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, the Fourth Military Medical University, Xi’an, China Background: It is unclear how the sympathetic nerves affect mesenchymal stem cell (MSC) migration and differentiation in bone regeneration. Distraction osteogenesis (DO) provides an ideal model of bone regeneration due to its unique nature as a self-engineering tissue. Objectives: To test the hypothesis that sympathetic nervous mediator norepinephrine (NE) inhibits MSC migration and osteogenic differentiation via 3-adrenergic receptor (adrb3) on MSCs in mandibular DO. Methods: Sympathetic denervation was performed by either resection of the sympathetic ganglion or administering sympathetic inhibitors in a rat model of mandibular DO, followed by immunohistochemistry and bone histomophometric analysis. The effects of NE to MSC migration and osteogenic differentiation were determined by Boyden chamber and osteogenic assays, respectively. Findings: Data showed that sympathetic denervation enhanced bone formation in mandibular DO, and depleted NE in tensile stress-induced bone callus and down-regulated adrb3 on MSCs, promoting MSC migration from perivascular regions to boneforming units. NE inhibited stroma-derived factor-1-induced MSC in vitro migration and expression of the migration-related gene MMP-2, whereas knockdown of abrd3 using siRNA abolished the inhibition of MSC migration. NE inhibited in vitro calcified node formation of MSCs and expression of the osteogenic marker ALP and RUNX2, whereas knockdown of abrd3 using siRNA abolished the inhibition of MSC osteogenic differentiation. Conclusions: Sympathetic nerves negatively regulate MSC migration and differentiation via NE/abrd3 in mandibular DO. It provides a possible way to enhance bone formation of DO, and may facilitate understanding the relationship between MSC mobilization and sympathetic nervous system in tissue regeneration processes. http://dx.doi.org/10.1016/j.ijom.2015.08.861 Using free navigation reference points and prefabricated bone plates for zygoma fracture model surgeries T. Wang 1,2,∗ , H. Ma 1,3 , C. Tseng 2 , Y. Chou 1,3 , K. Cai 2 1
Taipei Veterans General Hospital, Taipei, Taiwan National Central University, Taoyuan, Taiwan 3 National Yang-Ming University, Taipei, Taiwan 2
Background: Surgical navigation systems have been an important tool in maxillofacial surgery, helping surgeons create a presurgical plan, locate lesions, and provide guidance. Previous studies used predetermined markers as navigation references for the screw holes; however, unexpected situations may occur, making the predetermined surgical plan unreliable. Objectives: Instead of determining surgical reference positions preoperatively, a new method was developed that surgeons can use intraoperatively determined surface markers in a more flexible manner. Methods: Eight zygomatic fractures were created in four skull models, and preoperative CT image data were imported
Fig. 1. Preparing the STL image file.
Fig. 2. Using FNRPs and pre-bent bone plates for the zygoma reduction model surgery. (a) The STL image and the SLA model. Pre-bent plates were fixed on the SLA model. (b) The screw hole positions which will be on the dislocated zygoma (red dots on red zygoma) were calculated. During model surgery, the surgeon selected three marks on the zygoma and registered them as FNRPs (yellow dots on green zygoma) (c) Pre-bent bone plates from the SLA model. (d) A navigation system was used to mark and create screw holes.
into a newly developed navigation program for presurgical planning (Fig. 1). This program calculated the ideal positions to use as navigation references and as screw holes. During reduction, markers on fractured bone were selected, registered and calculated as free navigation reference points (FNRPs). These markers were used to monitor the position of the dislocated bone intraoperatively. Meanwhile, titanium bone plates were prefabricated on stereolithography (SLA) models for osteosynthesis (Fig. 2). Postoperative CT data were obtained, and surgical errors were evaluated. Findings and conclusions: The mean displacement was 0.83 ± 0.38 mm, and the average rotation around the x-axis was 0.66 ± 0.59◦ , y-axis was 0.77 ± 0.54◦ , and z-axis was 0.79 ± 0.42◦ . This is comparable to that of previous works. Combining presurgical planning and a newly developed navigation program to generate FNRPs that can assist in secondary zygoma reduction should be an accurate and practical method. Further application is necessitated to prove its clinical value. http://dx.doi.org/10.1016/j.ijom.2015.08.862
e163
Sympathetic nerves inhibit MSC migration and osteogenisis in distraction osteogenesis via norepinephrine/adrb3 L. Wang ∗ , Z. Du, J. Cao, D. Lei State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, the Fourth Military Medical University, Xi’an, China Background: It is unclear how the sympathetic nerves affect mesenchymal stem cell (MSC) migration and differentiation in bone regeneration. Distraction osteogenesis (DO) provides an ideal model of bone regeneration due to its unique nature as a self-engineering tissue. Objectives: To test the hypothesis that sympathetic nervous mediator norepinephrine (NE) inhibits MSC migration and osteogenic differentiation via 3-adrenergic receptor (adrb3) on MSCs in mandibular DO. Methods: Sympathetic denervation was performed by either resection of the sympathetic ganglion or administering sympathetic inhibitors in a rat model of mandibular DO, followed by immunohistochemistry and bone histomophometric analysis. The effects of NE to MSC migration and osteogenic differentiation were determined by Boyden chamber and osteogenic assays, respectively. Findings: Data showed that sympathetic denervation enhanced bone formation in mandibular DO, and depleted NE in tensile stress-induced bone callus and down-regulated adrb3 on MSCs, promoting MSC migration from perivascular regions to boneforming units. NE inhibited stroma-derived factor-1-induced MSC in vitro migration and expression of the migration-related gene MMP-2, whereas knockdown of abrd3 using siRNA abolished the inhibition of MSC migration. NE inhibited in vitro calcified node formation of MSCs and expression of the osteogenic marker ALP and RUNX2, whereas knockdown of abrd3 using siRNA abolished the inhibition of MSC osteogenic differentiation. Conclusions: Sympathetic nerves negatively regulate MSC migration and differentiation via NE/abrd3 in mandibular DO. It provides a possible way to enhance bone formation of DO, and may facilitate understanding the relationship between MSC mobilization and sympathetic nervous system in tissue regeneration processes. http://dx.doi.org/10.1016/j.ijom.2015.08.861 Using free navigation reference points and prefabricated bone plates for zygoma fracture model surgeries T. Wang 1,2,∗ , H. Ma 1,3 , C. Tseng 2 , Y. Chou 1,3 , K. Cai 2 1
Taipei Veterans General Hospital, Taipei, Taiwan National Central University, Taoyuan, Taiwan 3 National Yang-Ming University, Taipei, Taiwan 2
Background: Surgical navigation systems have been an important tool in maxillofacial surgery, helping surgeons create a presurgical plan, locate lesions, and provide guidance. Previous studies used predetermined markers as navigation references for the screw holes; however, unexpected situations may occur, making the predetermined surgical plan unreliable. Objectives: Instead of determining surgical reference positions preoperatively, a new method was developed that surgeons can use intraoperatively determined surface markers in a more flexible manner. Methods: Eight zygomatic fractures were created in four skull models, and preoperative CT image data were imported
Fig. 1. Preparing the STL image file.
Fig. 2. Using FNRPs and pre-bent bone plates for the zygoma reduction model surgery. (a) The STL image and the SLA model. Pre-bent plates were fixed on the SLA model. (b) The screw hole positions which will be on the dislocated zygoma (red dots on red zygoma) were calculated. During model surgery, the surgeon selected three marks on the zygoma and registered them as FNRPs (yellow dots on green zygoma) (c) Pre-bent bone plates from the SLA model. (d) A navigation system was used to mark and create screw holes.
into a newly developed navigation program for presurgical planning (Fig. 1). This program calculated the ideal positions to use as navigation references and as screw holes. During reduction, markers on fractured bone were selected, registered and calculated as free navigation reference points (FNRPs). These markers were used to monitor the position of the dislocated bone intraoperatively. Meanwhile, titanium bone plates were prefabricated on stereolithography (SLA) models for osteosynthesis (Fig. 2). Postoperative CT data were obtained, and surgical errors were evaluated. Findings and conclusions: The mean displacement was 0.83 ± 0.38 mm, and the average rotation around the x-axis was 0.66 ± 0.59◦ , y-axis was 0.77 ± 0.54◦ , and z-axis was 0.79 ± 0.42◦ . This is comparable to that of previous works. Combining presurgical planning and a newly developed navigation program to generate FNRPs that can assist in secondary zygoma reduction should be an accurate and practical method. Further application is necessitated to prove its clinical value. http://dx.doi.org/10.1016/j.ijom.2015.08.862