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Synaptology of synaptic glomeruli with substance P-immunoreactive central terminals in the lamina II of the chicken spinal cord
S216
1903
THE EFFECTS OF NOXIOUS MECHANICAL STIMULATION OF VARIOUS SEGMENTAL AREAS ON SYSTEMIC BLOOD PRESSURE AND HEART RATE IN ANESTHETIZED RATS WITH THE SPINAL CORD INTACT OR TRANSECTED AT THE CERVICAL LEVEL. ‘A. KIMURA. *H. OHSAWA. ‘A. SAT0 AND *x of Gerontologv. Sakaecho. It&$&&& SATO. ’RDartment of Autonomic Nervous System. Tokyo MetroDolitan &t&e Tokvo 173. *Laboratory of Physioloev. Tsukuba College of Technolma. Tsukuba-shi. Tsukuba 305. JaDan.
The effects of noxious mechanical stimulation of various segmental areas on systemic blood pressure and heart rate were examined in anesthetized rats with central nervous system (CNS) intact or acutely spinalized. In CNS intact rats pinching for 20 s applied on any segmental skin produced an increase in blood pressure and heart rate, indicating an existence of diffuse reflex organization, although stimulation of a forepaw and a hindpaw produced the largest responses, while stimulation of the low back and the thigh produced the least responses. In acutely spinalized rats pinching for 20 s applied on chest, abdomen and back of the body produced large increase in blood pressure and heart rate, while the limb and lower back stimulation induced only a small increase in blood pressure, but not increase in heart rate, indicating an existence of strong segmental organization in spinal rats. These results suggest the existence of the two types of reflex responses, the supraspinal and propriospinal ones, in the somato-cardiovascular reflex.
SOMATIC AFFERENT REGULATION OF CYTOTOXIC ACTIVITY OF SPLENIC NATURAL KILLER CELLS AND SPLENIC BLOOD FLOW IN ANESTHETIZED RATS. A. KIMURA. N. NAGAI AND A. SATO. Depmment of Au@nomic Nervous System. Tokyo Metropoli&n Institute of Geromecho. It&a&i-ku, Tokvo 173. Jw
1904
The effects of non-noxious and noxious mechanical stimulation of the skin on cytotoxic activity of splenic natural killer (NK) cells and splenic blood flow were examined in anesthetized rats. Bilateral brushing of body surface between the lateral chest and hindlimb for 30 min did not significantly influence cytotoxic activity of splenic NK cells, splenic blood flow or splenic sympathetic efferent nerve activity. Bilateral pinching stimulation of the skin of the hindpaws for 30 min reduced cytotoxic activity of splenic NK cells and splenic blood flow to 67 + 8% and 82 & 3% of the control value, respectively. Pinching the hindpaws for 30 min increased splenic sympathetic efferent nerve activity to 143 + 15% of control. Electrical stimulation (10 V, 10 Hz) of splanchnic nerve for 20 min produced a decrease in cytotoxic activity of splenic NK cells and splenic blood flow. The pinching of hindpaw induced suppression of cytotoxic activity of NK cells was abolished after surgical transection of splenic sympathetic nerve or spinal cord at the cervical level. These results indicate that the suppressionof cytotoxic activity of NK cells and splenic blood flow following noxious pinching stimulationis a supra-spinal reflexresponsemediatedvia splenicsympatheticnerve.
1905
SYNAFXUGY OF SYNAPTICGLOMERULI WIlli SUBSTANCE P-I MMlMRE4CTIVE CEN’IRAL TERMINALS IN IHE LMINA II OF ‘IliE CHICKENSPINALCORD. 1,
Yamanashi Med. Univ.,
Shimokato.
Tamaho, Nakakoma, Yamanashi,
400-38.
Japan.
We studied qmaptic architecture of the smptic glomeruli that contain substance P-immunoactive central terminals in the lamina II of the chicken cervical cord, using serial sectioning and postemhedding immunocytochemical technique. Each of the synaptic glomeruli contains l-5 central terminals end 14-80 peripheral elements. We found several synaptic relations between the central terminal and peripheral elements and between peripheral elements as follows: (1) asymmetrical axodendritic synapses between central axon terminals and peripheral dendrites, (2) asymterminals and postsynaptic vesicle-containing metrical qynapses between presynaptic central (3) symmetrical synapses between presynaptic vesicle-containirq profiles and postsynap profiles, (4) reciprocal synapses between central terminals and peripher31 vesicle tic central terminals, (5) symmetrical synapses between peripheral vesicle-containing profiles and containing profiles, symmetrical synapses between peripheral vesicle-containing profiles. peripheral dendrites , (6) lhese findings suggest that the primary afferent terminals which may convey noxious stimuli excite periphez31 elements on the one hand and may receive some modulations from the peripheral elements on the other hand in the synaptic glomeruli in the lamine II of the chicken spinal cord.
1903
THE EFFECTS OF NOXIOUS MECHANICAL STIMULATION OF VARIOUS SEGMENTAL AREAS ON SYSTEMIC BLOOD PRESSURE AND HEART RATE IN ANESTHETIZED RATS WITH THE SPINAL CORD INTACT OR TRANSECTED AT THE CERVICAL LEVEL. ‘A. KIMURA. *H. OHSAWA. ‘A. SAT0 AND *x of Gerontologv. Sakaecho. It&$&&& SATO. ’RDartment of Autonomic Nervous System. Tokyo MetroDolitan &t&e Tokvo 173. *Laboratory of Physioloev. Tsukuba College of Technolma. Tsukuba-shi. Tsukuba 305. JaDan.
The effects of noxious mechanical stimulation of various segmental areas on systemic blood pressure and heart rate were examined in anesthetized rats with central nervous system (CNS) intact or acutely spinalized. In CNS intact rats pinching for 20 s applied on any segmental skin produced an increase in blood pressure and heart rate, indicating an existence of diffuse reflex organization, although stimulation of a forepaw and a hindpaw produced the largest responses, while stimulation of the low back and the thigh produced the least responses. In acutely spinalized rats pinching for 20 s applied on chest, abdomen and back of the body produced large increase in blood pressure and heart rate, while the limb and lower back stimulation induced only a small increase in blood pressure, but not increase in heart rate, indicating an existence of strong segmental organization in spinal rats. These results suggest the existence of the two types of reflex responses, the supraspinal and propriospinal ones, in the somato-cardiovascular reflex.
SOMATIC AFFERENT REGULATION OF CYTOTOXIC ACTIVITY OF SPLENIC NATURAL KILLER CELLS AND SPLENIC BLOOD FLOW IN ANESTHETIZED RATS. A. KIMURA. N. NAGAI AND A. SATO. Depmment of Au@nomic Nervous System. Tokyo Metropoli&n Institute of Geromecho. It&a&i-ku, Tokvo 173. Jw
1904
The effects of non-noxious and noxious mechanical stimulation of the skin on cytotoxic activity of splenic natural killer (NK) cells and splenic blood flow were examined in anesthetized rats. Bilateral brushing of body surface between the lateral chest and hindlimb for 30 min did not significantly influence cytotoxic activity of splenic NK cells, splenic blood flow or splenic sympathetic efferent nerve activity. Bilateral pinching stimulation of the skin of the hindpaws for 30 min reduced cytotoxic activity of splenic NK cells and splenic blood flow to 67 + 8% and 82 & 3% of the control value, respectively. Pinching the hindpaws for 30 min increased splenic sympathetic efferent nerve activity to 143 + 15% of control. Electrical stimulation (10 V, 10 Hz) of splanchnic nerve for 20 min produced a decrease in cytotoxic activity of splenic NK cells and splenic blood flow. The pinching of hindpaw induced suppression of cytotoxic activity of NK cells was abolished after surgical transection of splenic sympathetic nerve or spinal cord at the cervical level. These results indicate that the suppressionof cytotoxic activity of NK cells and splenic blood flow following noxious pinching stimulationis a supra-spinal reflexresponsemediatedvia splenicsympatheticnerve.
1905
SYNAFXUGY OF SYNAPTICGLOMERULI WIlli SUBSTANCE P-I MMlMRE4CTIVE CEN’IRAL TERMINALS IN IHE LMINA II OF ‘IliE CHICKENSPINALCORD. 1,
Yamanashi Med. Univ.,
Shimokato.
Tamaho, Nakakoma, Yamanashi,
400-38.
Japan.
We studied qmaptic architecture of the smptic glomeruli that contain substance P-immunoactive central terminals in the lamina II of the chicken cervical cord, using serial sectioning and postemhedding immunocytochemical technique. Each of the synaptic glomeruli contains l-5 central terminals end 14-80 peripheral elements. We found several synaptic relations between the central terminal and peripheral elements and between peripheral elements as follows: (1) asymmetrical axodendritic synapses between central axon terminals and peripheral dendrites, (2) asymterminals and postsynaptic vesicle-containing metrical qynapses between presynaptic central (3) symmetrical synapses between presynaptic vesicle-containirq profiles and postsynap profiles, (4) reciprocal synapses between central terminals and peripher31 vesicle tic central terminals, (5) symmetrical synapses between peripheral vesicle-containing profiles and containing profiles, symmetrical synapses between peripheral vesicle-containing profiles. peripheral dendrites , (6) lhese findings suggest that the primary afferent terminals which may convey noxious stimuli excite periphez31 elements on the one hand and may receive some modulations from the peripheral elements on the other hand in the synaptic glomeruli in the lamine II of the chicken spinal cord.