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Synthesis and characterization of rutile phase of TiO2 nanoparticle for biological investigations
Abstracts
S77
Keywords: Sorbitol dehydrogenase, Organic solvent, ANS binding assay, Circular dichrosiom
E-mail addresses: [email protected] (R. Javaheri), [email protected] (M.M. Bagheri-Mohagheghi), [email protected] (M.M. Amir)
doi:10.1016/j.clinbiochem.2011.08.170
Introduction: Nanoparticles are very small materials with sizes less than 1000 nm which have different physical and chemical properties. Among the various kinds of nanoparticles, titanium dioxide (TiO2) has been used in different fields including biology. This nanoparticle has many applications such as sunscreen lotions, food products, painting colors, and photocatalytic analysis of organic materials as a semi-conductor. There are three crystal phases for TiO2: anatase, rutile and brookite of which the rutile phase has a higher chemical activity. In contrast to anatase nanoparticles which are produced in low temperatures, rutile ones can be obtained from a higher temperature. There are different ways to synthesize nanoparticles including chemical precipitation, microemulsion, hydrothermal, gel–sol and sol–gel. The sol–gel process is one of the most popular synthesis procedures of the titanium dioxide nanoparticle. Methods: In this study, ethanol, titanium IV isopropylate and acetyle acetone were mixed, then the nanoparticle TiO2 was obtained by an optimized protocol of a sol–gel process. Results: The obtained product was rutile (one of the TiO2 phases) which was determind by X-ray diffraction (XRD). The purity percentage of the product was over 95%. The average size of the nanoparticle was calculated at about 27 nm. In addition, the FTIR spectroscopy was used to investigate the structure of the rutile. In the FTIR spectrum of the rutile, a broad peak in the region 400–516 cm−1 demonstrates Ti–O–Ti vibrations. Conclusion: In this research, a rutile TiO2 nanoparticle was successfully synthesized. Another important conclusion is that the dried gel of TiO2 is transferred to the furnace if the floor surface for placing the nanoparticle (for instance, the fire-brick used in this research) is wider. However, there are reports of applications of TiO2 in biological and particularly medical studies.
Poster – [A-10-249-1] Antibacterial activity of ZnO nanofluids against E. coli O157:H7 Jalal Razieh, Saliani Mahsa, Kafshdar Goharshadi Elahe Department of Chemistry, Faculty of Sciences, Ferdowsi University of Mashhad, 91779, Iran E-mail addresses: [email protected] (J. Razieh), [email protected] (S. Mahsa) Introduction: At present, inorganic metal oxides like TiO2, MgO, CaO and ZnO have attracted interest as antimicrobial agents because of their safety and stability. They are also generally regarded as safe materials to human beings and animals. Among them, zinc oxide nanostructures are at the forefront of research due to their unique properties and widespread applications. In this study, the antibacterial behaviour of suspension of zinc oxide nanoparticles (ZnO nanofluids) against an important foodborne pathogen E. coli O157: H7 (NTCC. 12900) has been investigated. Methods: In order to determine MIC (minimum inhibitory concentration) of ZnO nanoparticles, the antibacterial tests were performed by measuring the growth curve of E. coli O157:H7 in the TSB broth medium in the presence of nanofluids containing 2, 1.5, 0.75, and 0.375 μg/ml of ZnO nanoparticles. Also, for characterizing the MBC (minimum bacterial concentration) of ZnO nanoparticles against E. coli O157:H7, bactericidal tests were performed in TSA medium at the same concentration of ZnO nanoparticles. Results: ZnO nanofluids with concentration of 1.5 μg/ml inhibited the bacterial growth completely in TSB broth medium. So, the MIC of ZnO nanoparticles against E. coli μg/ml was 1.5 μg/ml. Also, bacterial growth was completely inhibited in plates supplemented with 2 μg/ ml of ZnO nanoparticles. Hence, the MBC of ZnO nanoparticles against E. coli is 2 μg/ml. Discussion: ZnO nanoparticles show considerable antibacterial properties against E. coli and their antibacterial activity is dose dependent. So the antibacterial activity increases by increasing the nanoparticle concentration. These results suggest that ZnO nanoparticles can be used externally to control the spreading of bacterial infections. Thus, the preparation, characterization, surface modification, and functionalization of nanosized inorganic particles open the possibility of formulation of a new generation of bactericidal materials. Keywords: ZnO nanoparticles, Antibacterial activity, MIC, MBC, E. coli O157:H7
doi:10.1016/j.clinbiochem.2011.08.171
E Poster – [A-10-447-1] Synthesis and characterization of rutile phase of TiO2 nanoparticle for biological investigations Rahele Javaheria, Zahra Saghiria, Roshanak Ghafarian Ziraka, Mohammad Sarmad Nabavib, Masoud Saleh-Moghadama, Mohammad Mehdi Bagheri-Mohagheghic, Mohammad Malvandi Amira a Graduate Department of Biochemistry, Faculty of Science, Payame Noor University of Mashhad, Iran b Department of Natural Resources and Environmental Engineering, Faculty of Agricultural Sciences, Payame Noor University of Mashhad, Iran c School of Physics, Damghan University, Damghan, Iran
Keywords: Titanium dioxide nanoparticles, Sol–gel method, Rutile, XRD, FTIR doi:10.1016/j.clinbiochem.2011.08.172
E Poster – [A-10-449-2] The effects of TiO2 nanoparticles and doxorubicin complexes on the structure of DNA and inducing of apoptosis in MCF7 cell line Hekmat Azadeh, Saboury Ali Akbar University of Tehran, Tehran, Iran E-mail addresses: [email protected] (H. Azadeh), [email protected] (S.A. Akbar) Introduction: Breast cancer, which affects a significant percentage of human beings, takes place when abnormal cells grow out of control in one or both breasts. Anthracyclines, typically doxorubicin (DOX) are widely used antibiotics for medical treatments of breast cancer. The combinations of more than one drug can defeat adverse effects correlated to high doses of single drugs via multiple mechanisms such as sparing doses of all compounds. Methods: We studied the interaction between TiO2 nanoparticles (TiO2NPs), ct DNA and DOX using UV–visible and fluorescence spectroscopies at 37 °C. Growth inhibitory and apoptosis effects of DOX and TiO2NPs complexes on MCF7 cells were measured by MTT assay. Results and Conclusion: By the analysis of UV–visible titration and thermal denaturation studies of ct DNA, it was found that combination of DOX with TiO2NPs could form a new complex with double-helical ct DNA. In addition, the interaction of doxorubicin in various TiO2NP concentrations represented strong interaction between doxorubicin and
S77
Keywords: Sorbitol dehydrogenase, Organic solvent, ANS binding assay, Circular dichrosiom
E-mail addresses: [email protected] (R. Javaheri), [email protected] (M.M. Bagheri-Mohagheghi), [email protected] (M.M. Amir)
doi:10.1016/j.clinbiochem.2011.08.170
Introduction: Nanoparticles are very small materials with sizes less than 1000 nm which have different physical and chemical properties. Among the various kinds of nanoparticles, titanium dioxide (TiO2) has been used in different fields including biology. This nanoparticle has many applications such as sunscreen lotions, food products, painting colors, and photocatalytic analysis of organic materials as a semi-conductor. There are three crystal phases for TiO2: anatase, rutile and brookite of which the rutile phase has a higher chemical activity. In contrast to anatase nanoparticles which are produced in low temperatures, rutile ones can be obtained from a higher temperature. There are different ways to synthesize nanoparticles including chemical precipitation, microemulsion, hydrothermal, gel–sol and sol–gel. The sol–gel process is one of the most popular synthesis procedures of the titanium dioxide nanoparticle. Methods: In this study, ethanol, titanium IV isopropylate and acetyle acetone were mixed, then the nanoparticle TiO2 was obtained by an optimized protocol of a sol–gel process. Results: The obtained product was rutile (one of the TiO2 phases) which was determind by X-ray diffraction (XRD). The purity percentage of the product was over 95%. The average size of the nanoparticle was calculated at about 27 nm. In addition, the FTIR spectroscopy was used to investigate the structure of the rutile. In the FTIR spectrum of the rutile, a broad peak in the region 400–516 cm−1 demonstrates Ti–O–Ti vibrations. Conclusion: In this research, a rutile TiO2 nanoparticle was successfully synthesized. Another important conclusion is that the dried gel of TiO2 is transferred to the furnace if the floor surface for placing the nanoparticle (for instance, the fire-brick used in this research) is wider. However, there are reports of applications of TiO2 in biological and particularly medical studies.
Poster – [A-10-249-1] Antibacterial activity of ZnO nanofluids against E. coli O157:H7 Jalal Razieh, Saliani Mahsa, Kafshdar Goharshadi Elahe Department of Chemistry, Faculty of Sciences, Ferdowsi University of Mashhad, 91779, Iran E-mail addresses: [email protected] (J. Razieh), [email protected] (S. Mahsa) Introduction: At present, inorganic metal oxides like TiO2, MgO, CaO and ZnO have attracted interest as antimicrobial agents because of their safety and stability. They are also generally regarded as safe materials to human beings and animals. Among them, zinc oxide nanostructures are at the forefront of research due to their unique properties and widespread applications. In this study, the antibacterial behaviour of suspension of zinc oxide nanoparticles (ZnO nanofluids) against an important foodborne pathogen E. coli O157: H7 (NTCC. 12900) has been investigated. Methods: In order to determine MIC (minimum inhibitory concentration) of ZnO nanoparticles, the antibacterial tests were performed by measuring the growth curve of E. coli O157:H7 in the TSB broth medium in the presence of nanofluids containing 2, 1.5, 0.75, and 0.375 μg/ml of ZnO nanoparticles. Also, for characterizing the MBC (minimum bacterial concentration) of ZnO nanoparticles against E. coli O157:H7, bactericidal tests were performed in TSA medium at the same concentration of ZnO nanoparticles. Results: ZnO nanofluids with concentration of 1.5 μg/ml inhibited the bacterial growth completely in TSB broth medium. So, the MIC of ZnO nanoparticles against E. coli μg/ml was 1.5 μg/ml. Also, bacterial growth was completely inhibited in plates supplemented with 2 μg/ ml of ZnO nanoparticles. Hence, the MBC of ZnO nanoparticles against E. coli is 2 μg/ml. Discussion: ZnO nanoparticles show considerable antibacterial properties against E. coli and their antibacterial activity is dose dependent. So the antibacterial activity increases by increasing the nanoparticle concentration. These results suggest that ZnO nanoparticles can be used externally to control the spreading of bacterial infections. Thus, the preparation, characterization, surface modification, and functionalization of nanosized inorganic particles open the possibility of formulation of a new generation of bactericidal materials. Keywords: ZnO nanoparticles, Antibacterial activity, MIC, MBC, E. coli O157:H7
doi:10.1016/j.clinbiochem.2011.08.171
E Poster – [A-10-447-1] Synthesis and characterization of rutile phase of TiO2 nanoparticle for biological investigations Rahele Javaheria, Zahra Saghiria, Roshanak Ghafarian Ziraka, Mohammad Sarmad Nabavib, Masoud Saleh-Moghadama, Mohammad Mehdi Bagheri-Mohagheghic, Mohammad Malvandi Amira a Graduate Department of Biochemistry, Faculty of Science, Payame Noor University of Mashhad, Iran b Department of Natural Resources and Environmental Engineering, Faculty of Agricultural Sciences, Payame Noor University of Mashhad, Iran c School of Physics, Damghan University, Damghan, Iran
Keywords: Titanium dioxide nanoparticles, Sol–gel method, Rutile, XRD, FTIR doi:10.1016/j.clinbiochem.2011.08.172
E Poster – [A-10-449-2] The effects of TiO2 nanoparticles and doxorubicin complexes on the structure of DNA and inducing of apoptosis in MCF7 cell line Hekmat Azadeh, Saboury Ali Akbar University of Tehran, Tehran, Iran E-mail addresses: [email protected] (H. Azadeh), [email protected] (S.A. Akbar) Introduction: Breast cancer, which affects a significant percentage of human beings, takes place when abnormal cells grow out of control in one or both breasts. Anthracyclines, typically doxorubicin (DOX) are widely used antibiotics for medical treatments of breast cancer. The combinations of more than one drug can defeat adverse effects correlated to high doses of single drugs via multiple mechanisms such as sparing doses of all compounds. Methods: We studied the interaction between TiO2 nanoparticles (TiO2NPs), ct DNA and DOX using UV–visible and fluorescence spectroscopies at 37 °C. Growth inhibitory and apoptosis effects of DOX and TiO2NPs complexes on MCF7 cells were measured by MTT assay. Results and Conclusion: By the analysis of UV–visible titration and thermal denaturation studies of ct DNA, it was found that combination of DOX with TiO2NPs could form a new complex with double-helical ct DNA. In addition, the interaction of doxorubicin in various TiO2NP concentrations represented strong interaction between doxorubicin and