- Email: [email protected]
Taming torics and attacking astigmatism
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Abstracts / Contact Lens & Anterior Eye 35S (2012) e33–e50
ance is reasonably robust with respect to the day of week on which the assessment occurs, older participants may be more likely to complete all surveys than younger participants. http://dx.doi.org/10.1016/j.clae.2012.10.053
College of Optometry Forest Grove, more precise fitting of toric soft lenses on the astigmatic Oregon, USA patient. SATURDAY MAY 26, 2012 CONFERENCE SESSION 22: PRACTICAL CONTACT LENSES CONT’D, HALL 5, 11:00–11.50 CHAIR: JUDITH MORRIS
Can the effects of collagen cross-linking on human corneal cell population in-vitro?
http://dx.doi.org/10.1016/j.clae.2012.10.055
Ithar M. Beshtawi 1,∗ , Clare O’Donnell 2 , Chantal Hillarby 3 , Arun Brahma 4 , Fiona Carley 1,2,3,4 , Hema Radhakrishnan 1,2,3,4
Modern materials and their clinical implications
1
Faculty of Life Sciences, University of Manchester, Manchester, UK Optegra, Didsbury, Manchester, UK 3 Faculty of Medicine, University of Manchester, Manchester, UK 4 Manchester Royal Eye Hospital, Manchester, UK E-mail address: [email protected] (I.M. Beshtawi).
Ian Pyzer 1,2
2
Purpose: Corneal collagen cross-linking is a recently developed procedure to treat keratoconus. Research has been conducted to study the effects of treatment; in-vitro studies have been mainly done using animal models. We explored the feasibility of using eyebanked human corneas to examine the effects of collagen crosslinking on corneal cells in-vitro. Method: Two corneas were used, one cornea was crosslinked according to the standard protocol and the other cornea was exposed to riboflavin only as a control. Confocal microscopy was used to image the corneal layers and assess the endothelial cell and stromal keratocyte morphometry. TUNEL technique was used to assess apoptosis of the corneal cells. Results: The human cornea was successfully treated. Additionally we were able to collect morphometric data for the keratocytes and endothelial cells in both corneas. Anterior keratocyte density was 1037.6 ± 90.2 and 1379.5 ± 214.6 cells/mm2 in the crosslinked and control cornea respectively. Posterior keratocyte density was 432.7 ± 10.9 and 455.1 ± 58.4 cells/mm2 in the cross-linked and control cornea respectively and endothelial cell density was measured as 1744.6 ± 178.8 and 2282.9 ± 132.6 cells/mm2 in the cross-linked and control cornea respectively. Apoptosis detection by the TUNEL technique showed positive staining in the anterior portion of the cross-linked cornea only. No apoptotic nuclei were detected at any level in the control cornea. Conclusions: Human corneas in organ-culture can be successfully cross-linked allowing the effects of this treatment to be assessed in-vitro. The techniques developed in this preliminary study provide a model for assessing the effects of treatment and revised cross-linking protocols on layers of the human cornea invitro. SATURDAY MAY 26, 2012 CONFERENCE SESSION 21: PRACTICAL CONTACT LENSES, HALL 5, 09:30–10.30 ACCREDITED FOR 1 CL CET – DISPENSING OPTICIANS/CONTACT LENS SPECIALIST/OPTOMETRISTS COPE: 34134-CL CHAIR: JUDITH MORRIS
http://dx.doi.org/10.1016/j.clae.2012.10.054 Taming torics and attacking astigmatism Mark Andre ∗ , Patrick Caroline E-mail address: andrem@pacificu.edu (M. Andre). This lecture will examine the issue of how the astigmatic FAAO patient is managed with toric soft contact lenses. We will discuss contemporary fitting techniques that allow for a Pacific University
1
Johnson & Johnson, UK Johnson & Johnson, Ireland E-mail address: [email protected]. 2
In order to improve patient satisfaction with soft contact lenses, we need to better understand the various factors affecting their performance. Differences in material characteristics which affect patient experience will be discussed in detail, concentrating on those which have a direct bearing on comfort and discontinuation while also dispelling myths about others that do not. Oxygen, modulus, wettability, coefficient of friction, water content as well as differences in UV protection will be explored in this lecture. Understanding how the laboratory measurements for some of these the material properties are achieved including those that do not currently have an ISO standard, will be explored to allow practitioners to appreciate the methodology behind the data. The lecture will help the eye care professional considering both their new and existing wearers, to better appreciate how differences in design & material can provide significant benefits. SATURDAY MAY 26, 2012 CONFERENCE SESSION 23: PRACTICAL CONTACT LENSES CONT’D, HALL 5, 14:30–16.00 ACCREDITED FOR 1 CL CET – DISPENSING OPTICIANS/CONTACT LENS SPECIALISTS/OPTOMETRISTS CHAIR: NIGEL BURNETT HODD
http://dx.doi.org/10.1016/j.clae.2012.10.056 Getting started: A simple guide to fitting the keratoconic patient Paul Rose Visique Total Vision, New Zealand E-mail address: [email protected]. The lecture will take the fitter through a step by step fitting of the irregular cornea by firstly identifying the type of irregular cornea by correct interpretation of topographies, then choosing the correct design and the reasons why design is so important to maximise success rate. Then a 5 step fitting system will be illustrated which will approach the fitting in a systematic way. At each of the 5 steps, rules will be given to assist the fitter with choosing the correct parameters to maximise their success rate. Also fitting tips will be given to overcome fitting issues and problems. SATURDAY MAY 26, 2012 CONFERENCE SESSION 24: PRACTICAL GRAND ROUND, HALL 6, 16:30–17.30 CHAIR: DR CATHARINE CHISHOLM
http://dx.doi.org/10.1016/j.clae.2012.10.057
Abstracts / Contact Lens & Anterior Eye 35S (2012) e33–e50
ance is reasonably robust with respect to the day of week on which the assessment occurs, older participants may be more likely to complete all surveys than younger participants. http://dx.doi.org/10.1016/j.clae.2012.10.053
College of Optometry Forest Grove, more precise fitting of toric soft lenses on the astigmatic Oregon, USA patient. SATURDAY MAY 26, 2012 CONFERENCE SESSION 22: PRACTICAL CONTACT LENSES CONT’D, HALL 5, 11:00–11.50 CHAIR: JUDITH MORRIS
Can the effects of collagen cross-linking on human corneal cell population in-vitro?
http://dx.doi.org/10.1016/j.clae.2012.10.055
Ithar M. Beshtawi 1,∗ , Clare O’Donnell 2 , Chantal Hillarby 3 , Arun Brahma 4 , Fiona Carley 1,2,3,4 , Hema Radhakrishnan 1,2,3,4
Modern materials and their clinical implications
1
Faculty of Life Sciences, University of Manchester, Manchester, UK Optegra, Didsbury, Manchester, UK 3 Faculty of Medicine, University of Manchester, Manchester, UK 4 Manchester Royal Eye Hospital, Manchester, UK E-mail address: [email protected] (I.M. Beshtawi).
Ian Pyzer 1,2
2
Purpose: Corneal collagen cross-linking is a recently developed procedure to treat keratoconus. Research has been conducted to study the effects of treatment; in-vitro studies have been mainly done using animal models. We explored the feasibility of using eyebanked human corneas to examine the effects of collagen crosslinking on corneal cells in-vitro. Method: Two corneas were used, one cornea was crosslinked according to the standard protocol and the other cornea was exposed to riboflavin only as a control. Confocal microscopy was used to image the corneal layers and assess the endothelial cell and stromal keratocyte morphometry. TUNEL technique was used to assess apoptosis of the corneal cells. Results: The human cornea was successfully treated. Additionally we were able to collect morphometric data for the keratocytes and endothelial cells in both corneas. Anterior keratocyte density was 1037.6 ± 90.2 and 1379.5 ± 214.6 cells/mm2 in the crosslinked and control cornea respectively. Posterior keratocyte density was 432.7 ± 10.9 and 455.1 ± 58.4 cells/mm2 in the cross-linked and control cornea respectively and endothelial cell density was measured as 1744.6 ± 178.8 and 2282.9 ± 132.6 cells/mm2 in the cross-linked and control cornea respectively. Apoptosis detection by the TUNEL technique showed positive staining in the anterior portion of the cross-linked cornea only. No apoptotic nuclei were detected at any level in the control cornea. Conclusions: Human corneas in organ-culture can be successfully cross-linked allowing the effects of this treatment to be assessed in-vitro. The techniques developed in this preliminary study provide a model for assessing the effects of treatment and revised cross-linking protocols on layers of the human cornea invitro. SATURDAY MAY 26, 2012 CONFERENCE SESSION 21: PRACTICAL CONTACT LENSES, HALL 5, 09:30–10.30 ACCREDITED FOR 1 CL CET – DISPENSING OPTICIANS/CONTACT LENS SPECIALIST/OPTOMETRISTS COPE: 34134-CL CHAIR: JUDITH MORRIS
http://dx.doi.org/10.1016/j.clae.2012.10.054 Taming torics and attacking astigmatism Mark Andre ∗ , Patrick Caroline E-mail address: andrem@pacificu.edu (M. Andre). This lecture will examine the issue of how the astigmatic FAAO patient is managed with toric soft contact lenses. We will discuss contemporary fitting techniques that allow for a Pacific University
1
Johnson & Johnson, UK Johnson & Johnson, Ireland E-mail address: [email protected]. 2
In order to improve patient satisfaction with soft contact lenses, we need to better understand the various factors affecting their performance. Differences in material characteristics which affect patient experience will be discussed in detail, concentrating on those which have a direct bearing on comfort and discontinuation while also dispelling myths about others that do not. Oxygen, modulus, wettability, coefficient of friction, water content as well as differences in UV protection will be explored in this lecture. Understanding how the laboratory measurements for some of these the material properties are achieved including those that do not currently have an ISO standard, will be explored to allow practitioners to appreciate the methodology behind the data. The lecture will help the eye care professional considering both their new and existing wearers, to better appreciate how differences in design & material can provide significant benefits. SATURDAY MAY 26, 2012 CONFERENCE SESSION 23: PRACTICAL CONTACT LENSES CONT’D, HALL 5, 14:30–16.00 ACCREDITED FOR 1 CL CET – DISPENSING OPTICIANS/CONTACT LENS SPECIALISTS/OPTOMETRISTS CHAIR: NIGEL BURNETT HODD
http://dx.doi.org/10.1016/j.clae.2012.10.056 Getting started: A simple guide to fitting the keratoconic patient Paul Rose Visique Total Vision, New Zealand E-mail address: [email protected]. The lecture will take the fitter through a step by step fitting of the irregular cornea by firstly identifying the type of irregular cornea by correct interpretation of topographies, then choosing the correct design and the reasons why design is so important to maximise success rate. Then a 5 step fitting system will be illustrated which will approach the fitting in a systematic way. At each of the 5 steps, rules will be given to assist the fitter with choosing the correct parameters to maximise their success rate. Also fitting tips will be given to overcome fitting issues and problems. SATURDAY MAY 26, 2012 CONFERENCE SESSION 24: PRACTICAL GRAND ROUND, HALL 6, 16:30–17.30 CHAIR: DR CATHARINE CHISHOLM
http://dx.doi.org/10.1016/j.clae.2012.10.057