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Targeted PDT with phthalocyanine nanoparticles
Poster Abstracts
209
doi:10.1016/j.pdpdt.2011.03.282
P077
P076
Synthesis and characterization of gold nanoparticles based on water-soluble Purpurin-18-N-methyl-D-glucamine
Targeted PDT with phthalocyanine nanoparticles B. Kogan 1 , A. Pankratov 2 , A. Butenin 1 , R. Feyzulova 1 , T. Andreeva 2 , O. Yuzhakova 1 , R. Yakubovskaya 2 , V. Negrimovsky 1 , E. Lukyanets 1 , G. Vorozhtsov 1 1 2
State Research Center ‘‘NIOPIK’’, Moscow, Russia Hertsen Moscow Research Institute of Oncology, Russia
Nanoparticles (NPs) of highly hydrophobic photosensitizers (PSs) do not have photodynamic activity almost, while molecules of such PSs are very active often. This enables the targeted delivery of photosensitizer by means of NPs laser ablation. Tumor-bearing mice and NPs of different unsubstituted phthalocyanines were used in our studies. Suspension of NPs injected into the tail vein of mice after that tumor was irradiated with pulses of Q-switched ruby laser. The resulting fluorescence is indicative of the appearance of photosensitizer molecules in tumor. Distribution of fluorescence intensity in the depth of the tumor was measured. Examples of PDT treatment (mice-hybrid F1, females, sarcoma S-37) are presented in table. NPs of Zn phthalocyanine (ZnPc) were used. PDT with diode laser ( = 668 nm) carried out after pulse irradiation. Tumor growth inhibition (TGI (%) = (1 − V/V0 ) × 100) was measure of treatment effectiveness. Here V and V0 are tumor volumes in the study and control group respectively.
No. of group
ZnPc dose (mg/kg)
1 2 3 4
1 3
Number of pulses 10 10 10 0
PDT
+ + − +
TGI on the day after treatment, % 7
11
13
15
95 100 21 34
93 100 26 23
94 100 18 3
93 99 6 −17
The table shows that the effectiveness of PDT after pulse irradiation is very high (groups 1 and 2), whereas the pulsed irradiation or PDT alone is ineffective under these conditions (groups 3 and 4). In conclusion, the proposed method solves the problem of targeted delivery of hydrophobic photosensitizers and eliminates the side effects of phototoxicity. This work was supported by the Moscow Government. doi:10.1016/j.pdpdt.2011.03.283
Lkhagvadulam Byambajav, Kim Jung Hua, IL Yoon, ShimYoung Key PDT Research Institute, School of Nano System Engineering, Inje University, Gimh, Republic of Korea We have synthesized a new type of water soluble ionic photosensitizer Purpurin-18-N-methyl-D-glucamine. And it was transformed into a small gold nanoparticles stabilized by the photosensitizer without adding any particular reducing agents and surfactants. UV—vis spectroscopy, transmission electron microscopy (TEM), dynamic light scattering (DLS), TGA, XRD, ATR-IR, 1 H NMR, EA, MS were used to confirm the formation of gold nanoparticles and photosensitizer. doi:10.1016/j.pdpdt.2011.03.284 P078 Intracellular photodynamic therapy with novel photosensitisers loaded nanoparticule N. Saydan 1 , G. Asci 2 , M. Goksel 2 , A. Celik 2 1
Gebze Institute of Technology, Molecular Biology and Genetics, 41400-Kocaeli-TR, Turkey 2 Gebze Institute of Technology, Department of Chemistry, 41400 Kocaeli-TR, Turkey Photodynamic thereapy (PDT) is based on the use of photosentisicer and light to produce reactive oxygen species, which cause death of target cancer cells. Finding a suitable photosensitiser is crucial in improving the efficiency of PDT. Our aim is to develop phthalocyanine as a novel photosensitiser and polymeric nanoparticules (NP) as delivery systems for the PDT. For this purpose, a series of phthalocyanines were synthesized and encapsulated in polymeric nanoparticules. NPs were prepared by solvent emulsion method and characterized by Zeta-sizer and scanning electron microscope (SEM). Loading capacity and efficiency together with hydrophobic/hydrophilic characters of NP have been investigated. Preliminary results showed that application above-mentioned system in PDT studies with mesothelioma cells in vitro indicative of successful applications. doi:10.1016/j.pdpdt.2011.03.285
209
doi:10.1016/j.pdpdt.2011.03.282
P077
P076
Synthesis and characterization of gold nanoparticles based on water-soluble Purpurin-18-N-methyl-D-glucamine
Targeted PDT with phthalocyanine nanoparticles B. Kogan 1 , A. Pankratov 2 , A. Butenin 1 , R. Feyzulova 1 , T. Andreeva 2 , O. Yuzhakova 1 , R. Yakubovskaya 2 , V. Negrimovsky 1 , E. Lukyanets 1 , G. Vorozhtsov 1 1 2
State Research Center ‘‘NIOPIK’’, Moscow, Russia Hertsen Moscow Research Institute of Oncology, Russia
Nanoparticles (NPs) of highly hydrophobic photosensitizers (PSs) do not have photodynamic activity almost, while molecules of such PSs are very active often. This enables the targeted delivery of photosensitizer by means of NPs laser ablation. Tumor-bearing mice and NPs of different unsubstituted phthalocyanines were used in our studies. Suspension of NPs injected into the tail vein of mice after that tumor was irradiated with pulses of Q-switched ruby laser. The resulting fluorescence is indicative of the appearance of photosensitizer molecules in tumor. Distribution of fluorescence intensity in the depth of the tumor was measured. Examples of PDT treatment (mice-hybrid F1, females, sarcoma S-37) are presented in table. NPs of Zn phthalocyanine (ZnPc) were used. PDT with diode laser ( = 668 nm) carried out after pulse irradiation. Tumor growth inhibition (TGI (%) = (1 − V/V0 ) × 100) was measure of treatment effectiveness. Here V and V0 are tumor volumes in the study and control group respectively.
No. of group
ZnPc dose (mg/kg)
1 2 3 4
1 3
Number of pulses 10 10 10 0
PDT
+ + − +
TGI on the day after treatment, % 7
11
13
15
95 100 21 34
93 100 26 23
94 100 18 3
93 99 6 −17
The table shows that the effectiveness of PDT after pulse irradiation is very high (groups 1 and 2), whereas the pulsed irradiation or PDT alone is ineffective under these conditions (groups 3 and 4). In conclusion, the proposed method solves the problem of targeted delivery of hydrophobic photosensitizers and eliminates the side effects of phototoxicity. This work was supported by the Moscow Government. doi:10.1016/j.pdpdt.2011.03.283
Lkhagvadulam Byambajav, Kim Jung Hua, IL Yoon, ShimYoung Key PDT Research Institute, School of Nano System Engineering, Inje University, Gimh, Republic of Korea We have synthesized a new type of water soluble ionic photosensitizer Purpurin-18-N-methyl-D-glucamine. And it was transformed into a small gold nanoparticles stabilized by the photosensitizer without adding any particular reducing agents and surfactants. UV—vis spectroscopy, transmission electron microscopy (TEM), dynamic light scattering (DLS), TGA, XRD, ATR-IR, 1 H NMR, EA, MS were used to confirm the formation of gold nanoparticles and photosensitizer. doi:10.1016/j.pdpdt.2011.03.284 P078 Intracellular photodynamic therapy with novel photosensitisers loaded nanoparticule N. Saydan 1 , G. Asci 2 , M. Goksel 2 , A. Celik 2 1
Gebze Institute of Technology, Molecular Biology and Genetics, 41400-Kocaeli-TR, Turkey 2 Gebze Institute of Technology, Department of Chemistry, 41400 Kocaeli-TR, Turkey Photodynamic thereapy (PDT) is based on the use of photosentisicer and light to produce reactive oxygen species, which cause death of target cancer cells. Finding a suitable photosensitiser is crucial in improving the efficiency of PDT. Our aim is to develop phthalocyanine as a novel photosensitiser and polymeric nanoparticules (NP) as delivery systems for the PDT. For this purpose, a series of phthalocyanines were synthesized and encapsulated in polymeric nanoparticules. NPs were prepared by solvent emulsion method and characterized by Zeta-sizer and scanning electron microscope (SEM). Loading capacity and efficiency together with hydrophobic/hydrophilic characters of NP have been investigated. Preliminary results showed that application above-mentioned system in PDT studies with mesothelioma cells in vitro indicative of successful applications. doi:10.1016/j.pdpdt.2011.03.285