TBARS production and dopamine uptake in rat striatai slices after preincubation: effect of MPTP

thedobmine ; take s&m. TEARS end PHjdopaminc ($H]OA) uptake wxe measured befwe or ether incuba,lon 137%. 150 mini wi(h w WRhou, ,he presence a, differen, coecen,ra,ion~ of MPTF! Pieincubation ot slices induced an overproductionof TSAAS and a ma@ reductionof [3H]DAuptake The addilion of MPTP in premoubaliOnmedium suppressed the ownproduction of TSARS a, 1W4 M and intensified the inhibtion of pH]DA uptake in e dose-dependentmanner MPTP effe-z,was totally mh~bi,ed by Ldeprenyl (MAO-B inhibitor). These data ehcw that duiig C&Icubatio”. the oxidation of MPTP to MPP+ lead to an unexpectedpa. tectti against peroxidati~, whereas this compxmd 1sdeeuibed es inducing free radical production (31.

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A&words: MPTP: TBARS: dopamine uptake: slice% prelnsubstion

M. Hurb6nkov6.A. Kai#bd. I. GrambliCkw6. Cww-Onent of -

Asbertos belongs ,O adverssfac,ors of acupatana, oenmg and #eneml environmerewhioh may oause asbestosis. branchal carc~oma and nwsotheliimas of pleura and peritoneum. evemuallq mesmhe 11oma5of other orgaos after long ,*,m *xpas”re. Conseq”en,~. S”bS,C w,es of asbeet.% have bee” increasir& used in industw. This swdy was designed to investi#ate diiferences of some lava#e parameters b&was” convol animab and those which were dusted by long end shwt amorite and wollastonite fiiers. We iNtilled dusts invatracheel to W,s,e, rats during ten weeks (a 1 m#Awek]. Three “anti ef,er (he first i”sWetion ,he animals were lavapad and the fdlowin# part*,% were inw.tloated: the numberof 01 b+ortcboabecfar leve#e (GAL),-the numbef of alveolar m&@ages (AMUml GAL, phapocynio anivtv. viabili of AM. AMlpranuloeyle radio in bva#e IBid. Iaotatedehydrogenase(LDH). acid phosphatsseIACW.TNF-sl~ha and IFNgamma release bylhe lavaps leukocyles.While insIiWltin of wollastonib bng and short f&redid no, influencesigmficendyIheperame,e~~swtied. beween amosite lbnp and shonfibargroup marked differences in prame,ers studied were found, the br+# fibers more effective Cornoared with the control. in ohewxvbc ecttihr and AcP release wre noted. In the bn# amo& iit& g&p signiamly de. creased number of AM/ml GAL. depressionof phagocylic activihland

leukocvtes/ml