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Techniques for the Analysis of Complex Genomes
Comp. Biochem. Physiol. Vol. 105B, No. 2, pp. 421-422, 1993
Printed in Great Britain
BOOK REVIEWS
Lipoprotein Analysis; a Practical Approach--Edited by C. A. CONVERSE and E. R. SKINNER.251 pp. 1992. IRL/Oxford University Press, Oxford. $65. This volume deals with lipoprotein (L) separation and analysis in clinical studies; separation of human apoL; immunological methods for quantifying apoL and L; separation of HDL and LDL; L turnover and metabolism; L-receptor interactions; assay of L-lipase and hepatic lipase; cholesterol esterifying enzymes; assay of 3-hydroxy-methylglutaryl coenzyme A (HMG-CoA) reductase; analysis of tissue L. Lipoproteins are important in heart disease, stroke, atheroma and other diseases and a better understanding of their role will help in controlling these malfunctions. Intraeellular Protein Degradation. "In Focus" Series---By F. J. DOHERTYand R. J. MAYER. 61 pp. 1992. IRL/Oxford University Press, Oxford. $14.95.
studying the physicochemical properties of proteins and their underlying molecular structure. The chapters deal with thermodynamics; chemical potential of proteins (P); simultaneous equilibria of a P with several ligands; binding of one ligand at multiple P sites; multiple binding with ligand interactions; ligand distribution; binding by multimer P; hemoglobin; equilibria involving covalent and non-covalent ligands; intramolecular and solvent interactions of P; transfer of P to apolar media and the dynamic interaction of P and membranes; detection and measurement of the statics and dynamics of P-ligand and P-P associations; effects of temperature and pressure on molecular associations and on single-chain P; dissociation of P dimers; effect of pressure on oligomeric P and P aggregates; biological specificity and ligand binding. The approach accentuates the basic principles and the distinction between what can and cannot be taken for granted. Biosynthesis of Tetrapyrroles---Edited by P. M. JORDAN.309 pp. 1992. Elsevier, Amsterdam. D.F1.225. $115.50.
Proteins are continually broken down and synthesized in the body. Protein degradation is involved in cellular homeostasis, degenerative (Alzheimer's) and viral disease. The chapters deal with intracellular proteolysis; lysosomes; ubiqultin pathway; molecular determination of protein half-life. The book is aimed at advanced undergraduates.
This new edition brings up to date the information on gene structure and transcription machinery in prokaryotes; regulation of transcription in prokaryotes; gene structure and transcription machinery in eukaryotes; regulation of transcription in eukaryotes; The new advances in RNA processing, DNA-binding proteins, are described. The book is aimed at advanced undergraduates.
Studies on the biosynthesis of tetrapyrroles showed that glycine was the major source of nitrogen of protoporphyrin (P)-IX via 5-aminolaevulinic acid (DALA). P-IX is an essential intermediate of chlorophyll, bacterial chlorophyll and vitamin Bt2. A new development is the discovery that factor F430 is a tetrapyrrole nickel complex, an essential growth factor for the Archaobacteria, with the methylation of carbon atoms at varying positions of the macrocyclic system. There has also been the discovery of a second pathway for the synthesis of DALA based not on glycine but on glutamate. This book deals with synthesis of DALA and its transformation into uroporphyrinogen III (U-III); conversion of U-III into haem; synthesis of vitamin Bt2; biochemistry of coenzyme F430 involved in methagenesis; biosynthesis and regulation of photosynthetic pigment formation; structure and biosynthesis of bacteriochlorophylls; the genes of tetrapyrrole biosynthesis;
Techniques for the Analysis of Complex Genomes---Edited by R. ANAND. 239 pp. 1992. Academic Press, London. Paperback £19.50.
Dibenzanthracenes and Environmental Carcinogenesis---By W. F. KARCHER.271 pp. 1992. Cambridge University Press, Cambridge. £75.
DNA cloning technology has made great advances with the use of the yeast artificial chromosome (YAC) and bacteriophage PI. This book describes the practical methods for long-range physical mapping of the human genome; analysis of genomic DNAs by pulsed-field gel electophoresis; bacteriophage P 1 cloning system; construction, characterization and screening of YAC libraries; cloning human telomeres in YAC; structural instability of YAC clones and the use of recombinant deficient yeast host strains; the analysis of YAC clones; high-density gridded YAC filters; transcribed sequences within YACs, HTF island cloning and cDNA library screening; YAC modifying vectors; reconstructions of megabase-sized genomic regions using overlapping YACs; generation of region-specific probes by microdissection and universal enzymatic DNA amplification.
In the 1930s the dibenzanthracenes (D) were identified as skin carcinogens. This book deals with the structure, nomenclature and physichemical properties of D; preparation of D (D, benzonaphthacene, pentacene); occurrence and exposure; analysis and spectra; metabolism and cellular interactions; mutagenicity and toxicity; carcinogenicity; structure-activity relationships. D have been associated with scrotal cancers of chimney-sweeps; cancer in workers in the coke-oven industry, coal gasification and steel workers; also in those involved in the use of mineral oils and cutting oils.
Gene Structure and Transcription; Second Edition. "In Focus" Series--By T. BEEBEEand J. BURKE. 89 pp. 1992. IRL/Oxford University Press, Oxford. $14.95.
Protein Interactions--By GREGORIOWEBER. 293 pp. 1992. Routledge, Chapman & Hall, New York, U.S.A. $55, Canada $68.95. This book summarizes the author's 40 years' experience of 421
Release of Genetically Engineered and other Micro-organisms--Edited by J. C. FRY and M. J. DAY. 178 pp. 1992. Cambridge University Press, Cambridge. £45. The risks and legislation concerning the release of genetically engineered microorganisms (GEM) and the use of specifically selected un-engineered organisms is discussed in this book. The chapters deal with rhizobacteria as soil innoculants in agriculture; bacteria and agricultural animals-survival and gene transfer; use of xenobiotic degrad-
Printed in Great Britain
BOOK REVIEWS
Lipoprotein Analysis; a Practical Approach--Edited by C. A. CONVERSE and E. R. SKINNER.251 pp. 1992. IRL/Oxford University Press, Oxford. $65. This volume deals with lipoprotein (L) separation and analysis in clinical studies; separation of human apoL; immunological methods for quantifying apoL and L; separation of HDL and LDL; L turnover and metabolism; L-receptor interactions; assay of L-lipase and hepatic lipase; cholesterol esterifying enzymes; assay of 3-hydroxy-methylglutaryl coenzyme A (HMG-CoA) reductase; analysis of tissue L. Lipoproteins are important in heart disease, stroke, atheroma and other diseases and a better understanding of their role will help in controlling these malfunctions. Intraeellular Protein Degradation. "In Focus" Series---By F. J. DOHERTYand R. J. MAYER. 61 pp. 1992. IRL/Oxford University Press, Oxford. $14.95.
studying the physicochemical properties of proteins and their underlying molecular structure. The chapters deal with thermodynamics; chemical potential of proteins (P); simultaneous equilibria of a P with several ligands; binding of one ligand at multiple P sites; multiple binding with ligand interactions; ligand distribution; binding by multimer P; hemoglobin; equilibria involving covalent and non-covalent ligands; intramolecular and solvent interactions of P; transfer of P to apolar media and the dynamic interaction of P and membranes; detection and measurement of the statics and dynamics of P-ligand and P-P associations; effects of temperature and pressure on molecular associations and on single-chain P; dissociation of P dimers; effect of pressure on oligomeric P and P aggregates; biological specificity and ligand binding. The approach accentuates the basic principles and the distinction between what can and cannot be taken for granted. Biosynthesis of Tetrapyrroles---Edited by P. M. JORDAN.309 pp. 1992. Elsevier, Amsterdam. D.F1.225. $115.50.
Proteins are continually broken down and synthesized in the body. Protein degradation is involved in cellular homeostasis, degenerative (Alzheimer's) and viral disease. The chapters deal with intracellular proteolysis; lysosomes; ubiqultin pathway; molecular determination of protein half-life. The book is aimed at advanced undergraduates.
This new edition brings up to date the information on gene structure and transcription machinery in prokaryotes; regulation of transcription in prokaryotes; gene structure and transcription machinery in eukaryotes; regulation of transcription in eukaryotes; The new advances in RNA processing, DNA-binding proteins, are described. The book is aimed at advanced undergraduates.
Studies on the biosynthesis of tetrapyrroles showed that glycine was the major source of nitrogen of protoporphyrin (P)-IX via 5-aminolaevulinic acid (DALA). P-IX is an essential intermediate of chlorophyll, bacterial chlorophyll and vitamin Bt2. A new development is the discovery that factor F430 is a tetrapyrrole nickel complex, an essential growth factor for the Archaobacteria, with the methylation of carbon atoms at varying positions of the macrocyclic system. There has also been the discovery of a second pathway for the synthesis of DALA based not on glycine but on glutamate. This book deals with synthesis of DALA and its transformation into uroporphyrinogen III (U-III); conversion of U-III into haem; synthesis of vitamin Bt2; biochemistry of coenzyme F430 involved in methagenesis; biosynthesis and regulation of photosynthetic pigment formation; structure and biosynthesis of bacteriochlorophylls; the genes of tetrapyrrole biosynthesis;
Techniques for the Analysis of Complex Genomes---Edited by R. ANAND. 239 pp. 1992. Academic Press, London. Paperback £19.50.
Dibenzanthracenes and Environmental Carcinogenesis---By W. F. KARCHER.271 pp. 1992. Cambridge University Press, Cambridge. £75.
DNA cloning technology has made great advances with the use of the yeast artificial chromosome (YAC) and bacteriophage PI. This book describes the practical methods for long-range physical mapping of the human genome; analysis of genomic DNAs by pulsed-field gel electophoresis; bacteriophage P 1 cloning system; construction, characterization and screening of YAC libraries; cloning human telomeres in YAC; structural instability of YAC clones and the use of recombinant deficient yeast host strains; the analysis of YAC clones; high-density gridded YAC filters; transcribed sequences within YACs, HTF island cloning and cDNA library screening; YAC modifying vectors; reconstructions of megabase-sized genomic regions using overlapping YACs; generation of region-specific probes by microdissection and universal enzymatic DNA amplification.
In the 1930s the dibenzanthracenes (D) were identified as skin carcinogens. This book deals with the structure, nomenclature and physichemical properties of D; preparation of D (D, benzonaphthacene, pentacene); occurrence and exposure; analysis and spectra; metabolism and cellular interactions; mutagenicity and toxicity; carcinogenicity; structure-activity relationships. D have been associated with scrotal cancers of chimney-sweeps; cancer in workers in the coke-oven industry, coal gasification and steel workers; also in those involved in the use of mineral oils and cutting oils.
Gene Structure and Transcription; Second Edition. "In Focus" Series--By T. BEEBEEand J. BURKE. 89 pp. 1992. IRL/Oxford University Press, Oxford. $14.95.
Protein Interactions--By GREGORIOWEBER. 293 pp. 1992. Routledge, Chapman & Hall, New York, U.S.A. $55, Canada $68.95. This book summarizes the author's 40 years' experience of 421
Release of Genetically Engineered and other Micro-organisms--Edited by J. C. FRY and M. J. DAY. 178 pp. 1992. Cambridge University Press, Cambridge. £45. The risks and legislation concerning the release of genetically engineered microorganisms (GEM) and the use of specifically selected un-engineered organisms is discussed in this book. The chapters deal with rhizobacteria as soil innoculants in agriculture; bacteria and agricultural animals-survival and gene transfer; use of xenobiotic degrad-