Ten cases of oral cancers treated with concurrent chemoradiotherapy using superselective intra-arterial infusion via superficial temporal and occipital arteries

Poster Session p53-mediated cell cycle arrest. In addition, increased expression of MUC1 in cancer cells that promotes cancer cell invasion through beta-catenin, resulting in the initiation of epithelial-mesenchymal transition which promotes the formation of metastases. However, little data are available regarding the role of MUC1 in oral cancers. Then, this study examined the impact of MUC1 against oral squamous cell carcinoma. Materials and Methods: Five human oral squamous cell carcinoma (HOSCC) cell lines, HSC-2, HSC-3, HSC-4, Ca9-22 and SAS, were used in this study. Caspase activity was determined by Caspase-Glo. We measured the expression of MUC1 mRNA and protein by RT-PCR and Western blotting in oral cancer cells, respectively. Morphological change was observed by light microscopy. Results: It was demonstrated that MUC1 mRNA and protein certainly expressed on 5 oral cancer cells. As a result of real time qRT-PCR, MUC1 expression level was the highest in HSC-4 cells. MUC1 knockdown with MUC1 siRNA in HSC-4 cells revealed to decrease the number of HSC-4 cells. Caspase activity was also determined. Expression of MUC1 was increased in the hypoxia condition. We observed concomitant increase of HIF-1alpha and MUC1 in HSC-4 cells. Conclusion: These findings suggested that MUC1 might serve as a potential therapeutic target in HOSCC. References: 1. Sebastien Aubert, Valerie Fauquette, Brigitte Hemon, et al. : MUC1, a New Hypoxia Inducible Factor Target Gene, Is an Actor in Clear Renal Cell Carcinoma Tumor Progression. Cancer Res 69: 5707-5715, 2009. 2. Hollingsworth MA, Swanson BJ. Mucins in cancer: protection and control of the cell surface. Nat Rev Cancer 4: 45-60, 2004.

POSTER 90 Ten cases of oral cancers treated with concurrent chemoradiotherapy using superselective intra-arterial infusion via superficial temporal and occipital arteries S. Ibaragi: Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, N. Yoshioka, T. Okui, Y. Kunisada, A. Morisawa, Y. Fujii, K. Kishimoto, T. Shimo, K. Mitsudo, I. Tohnai, A. Sasaki Purpose: Post-operative problems of advanced oral cancer are consisted from functional disorder of mastication, swallowing and speech, and cosmetic disturbance. One of the therapeutic strategies to solve these problems and preserve organs is concurrent chemoradiotherapy using superselective intra-arterial infusion. We have applied this therapy to the treatment of advanced oral cancers from 2011. The purpose of this study was to investigate the therapeutic results and complications of treatment with this therapy for advanced oral cancers. e-102

Materials and Methods: Between January 2011 and March 2014, 10 patients (4 male and 6 female) with T2, T3 or T4 oral squamous cell carcinomas (T2: 2 cases, T3: 2 cases, T4: 6 cases) underwent chemoradiotherapy using superselective intra-arterial infusion via superficial temporal and occipital arteries with docetaxel (DOC) and cisplatin (CDDP). Treatment consisted of superselective intra-arterial infusions (DOC: total 60-70 mg/m2, CDDP: total 150-175 mg/m2) and daily concurrent radiation therapy (2 Gy/ fraction/ day, total 60-70 Gy) for 6-7 weeks. Toxicities encountered during therapy were evaluated according to the National Cancer Institute Common Terminology Criteria for Adverse Events version 4.0. Results: The patients’ ages ranged from 37 to 74 years (mean: 58.8 years). The primary tumor sites included the tongue (5 cases), upper gingiva (4 cases) and floor of mouth (1 case). The therapy as described above was completed in 9 cases and Seldginger’s method was also need to be used in 1 case because of vascular occlusion of lingual artery. After completion of the therapy, primary site complete response was achieved in 9 of 10 cases (90%). Grade3 mucositis and dysphagia occurred in all cases (100%). Grade3 dermatitis occurred in 6 cases (60%). Grade3 nausea/vomiting occurred in 1 case (10%) All other toxicities were grade 2 or less. Five of 10 patients underwent neck dissection after the therapy. The median follow-up for all patients was 24.9 months. During follow-up, all patients were alive without disease. Conclusions: Chemoradiotherapy using intra-arterial infusion provided good overall survival and locoregional controls without major complications. References: 1. Organ preservation with daily concurrent chemoradiotherapy using superselective intra-arterial infusion via a superficial temporal artery for T3 and T4 head and neck cancer. Mitsudo K, Shigetomi T, Fujimoto Y, Nishiguchi H, Yamamoto N, Furue H, Ueda M, Itoh Y, Fuwa N, Tohnai I. Int J Radiat Oncol Biol Phys. 79, 1428-35, 2011. 2. Arterial chemoradiotherapy for locally advanced tongue cancer: analysis of retrospective study of therapeutic results in 88 patients. Fuwa N, Kodaira T, Furutani K, Tachibana H, Nakamura T, Nakahara R, Tomoda T, Inokuti H, Daimon T. Int J Radiat Oncol Biol Phys. 72, 1090-1100, 2008

POSTER 91 Two cases of multiple idiopathic root resorption in maxilla K. Sugahara: Tokyo dental college, Y. Hamada, M. Takano, C. Saito, T. Shibahara Multiple idiopathic root resorption (MIRR) is a rare disease in which the roots of multiple teeth are resorbed within a short period of time without any particular cause. We present 2 cases of MIRR in maxilla. Case 1: A 39-year-old woman was referred to our clinic with a chief complaint of discomfort in the right maxillary AAOMS  2014