The application of fuchsine dyes in the detection of naphthalene derivatives in thin-layer chromatography

MICROCHEMICAL

13,405-406

JOURNAL

(1968)

The Application of Fuchsine Dyes in the Detection of Naphthalene Derivatives in Thin- Layer Chromatography I J~ZEF Department

of Organic

SLIWIOK

Chemistry,

Received

University,

January

Katowice,

Poland

16, 1968

The conditions of the detection and the characteristics of the used fuchsine dyes were the same as described in a previous publication ( 1). The Kieselgel G was producted by Merck-Darmstadt. Plates were spread at 0.7 mm thickness according to standard procedures with “Camag” apparatus. The plates were activated at 110°C for 30 minutes immediately before use. Benzene and acetone in a volume ratio of 24:l was used for the mobile phase. The chromatograms were developed to the height of 14 cm at temperature 21°C in a period of 30 minutes. Chemicals used for the chromatography analysis shown in Table 1 were pure. The plates were sprayed with water solutions of TABLE DEVELOPING

ABXITIES

OF THE

I

APPLIED

NAPHTHALENE

Substances

Naphthalene a-Bromonaphthalene a-Nitronaphthalene a-Naphthylamine a-Naphthol a-Acetylonaphthalene a-Naphthalene sulfonic a-Methylnaphthalene P-Methoxynaphthalene P-Naphthylamine p-Naphthol @-Methylnaphthalene 1, 2-diaminonaphthalene 2,6-dimethylnaphthalene

acid

FUCHSINE

DYES

FOR SINGLE

DERIVATIVES

New fuchsine

Basic fuchsine

(a)

(a)

-

-

10 5 5 8 5 5 5 -

15 10 10 15 10 12 15 -

0 0.35 0.38 0.64

10

15

0.10 -

0.31 0.32 0.37

406

iLIWIOK

the above-mentioned fuchsine dyes. White or gray spots of the separated naphthalene derivative appeared immediately on the red background. Table 1 shows the results. In the present investigation it was found that substances without functional groups or with polarity groups, cannot be detected by fuchsine dyes. The quality and the quantity of functional groups in the compounds have influence on the detecting abilities of the fuchsine dyes. The new fuchsine was a more effective developing medium than basic fuchsine. REFERENCE 1.

J., The application of fuchsine acids by thin-layer chromatography.

SLIWIOK,

dyes in the detection of higher fatty Micro&em. J. 13, 108-110, 1968.