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The biodegradable polycaprolactone three-dimensional scaffold fabricated by melt spinning and multilayer deposition for bone tissue engineering
Free Papers—Poster Presentations tooth germs were enzymatically separated from dental epithelium and cultured in the absence and presence of Shh and BMPs. Odontoblast differentiation markers were examined by Northern Blot analysis. Results: Shh mRNA was produced by inner dental epithelium; the expression was not constant but varied with development and cytodifferentiation of ameloblasts along the cusp-to-cervix axis. Inner dental epithelium expressed the Shh receptor Patched-2, indicating autocrine signalling loops. Shh protein (SHH), but not RNA, was present in underlying differentiating preodontoblasts, probably resulting from gradual diffusion from the epithelial layers. Patched-1 was expressed in dental mesenchyme, reflecting paracrine loops of action. Interestingly, BMPs were also expressed in both preameloblasts and preodontoblasts, indicating functional interactions between the two signalling molecules during their differentiation. In dental mesenchyme primary cultures, SHH upregulated the expression levels of odontoblast differentiation markers including Alp and Osteopontin. The stimulatory effect of SHH on ALPase activity was synergistically enhanced by BMP2 or BMP4. Conclusion: Our data show that inner dental epithelium is a highly-regulated and Shh-expressing structure. SHH directly acts on dental mesenchyme and regulates odontoblast differentiation in cooperation with BMP2 and BMP4.
poly-vinyl template. The pattern of the layer was a grid pattern with average gaps area of 500 m2 . The contact points of each filament were connected by stamping in the warm water. The multilayer scaffolds were made by depositing of monolayer scaffolds and stamping. The physical properties and biocompatibilities of the scaffolds were evaluated. Methods: The physical properties of the scaffolds including the surface morphologies, mechanical strength and degradation behaviours were assessed. The cellscaffold constructs were made by seeding mouse osteoblast cell lines at 1 × 105 cells/scaffold and cultured for 21 days. MTT and enzyme-linked immunosorbent assay test of alkaline phosphatase and osteocalcin were performed to evaluate the cell proliferation and differentiation respectively. The alizarin red S staining was used to quantify the mineralisation of the constructs. Results: The scaffolds had interconnecting structures, pore size and surface morphology suitable for cell attachment with sufficient strength for using in the oral region. The cells proliferated well during culture period. The increase of alkaline phosphatase activity, osteocalcin level and the degree of mineralisation indicated the well differentiation. Conclusion: The study demonstrated that the scaffolds fabricated by MSMD technique were compatible for bone regeneration. doi:10.1016/j.ijom.2009.03.691
N. Thuaksuban ∗ , T. Nuntanaranont, W. Pattanachot, S. Suttapreyasri, K. Sutin Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Prince of Songkla University, Hatyai, Songkhla, Thailand
Background and Objectives: The novel technique of melt spinning and multilayer deposition (MSMD) was practical and economical for fabricating the biodegradable polycaprolactone (PCL) three-dimensional scaffolds. In brief, the PCL monofilaments were fabricated by melting and extruding through custom made extruder followed by stretching. The filaments were arranged to form the monolayer scaffolds on the custom made
without history of any systemic disease. TLRs expression in inflammatory granulation tissue and atheromatous plaques was analysed using quantitative reverse transcriptase-polymerase chain reaction profiling. Results: TLR3 and TLR 7 in granulation tissue showed a relatively high differentiation—TLR3 variance reached 1.1, and TLR7 variance was 0.95 (standard deviations were 1.049 and 0.97, respectively), so in case of these two TLRs neither median nor mean values should be considered reliable. The mean mRNA copy number of TLR1 is higher in granulation tissue than in atheromatous plaque whereas TLR2, TLR6 and TLR7 readings in granulation tissue are noticeably lower than those in atheromata. In the case of TLR3, TL4, TLR5 and TLR8, granular tissue and atheroma readings are practically equal. Conclusion: TLR2 seems to be the most sensitive marker of the relationship between carotid atherosclerosis and periodontal disease. doi:10.1016/j.ijom.2009.03.692
P220 The role of interferon-gamma in the aetiology of atheroma and periodontitis I. Niedzielska 1,∗ , M. Tkacz 2 Department of Craniomaxillofacial Surgery, Silesian Medical University, Katowice, Poland 2 Institute of Computer Science, University of Silesia, Poland
1
doi:10.1016/j.ijom.2009.03.690
P218 The biodegradable polycaprolactone threedimensional scaffold fabricated by melt spinning and multilayer deposition for bone tissue engineering
595
P219 The role of toll-like receptors in carotid atherosclerosis and periodontal disease I. Niedzielska 1,∗ , M. Tkacz 2 , H. Borgiel-Marek 1 1 Department of Craniomaxillofacial Surgery, Silesian Medical University, Katowice, Poland 2 Institute of Computer Science, University of Silesia, Poland
Background and Objectives: The role of toll-like receptors (TLRs) in atherosclerosis and periodontosis has been investigated but is not clear. Aim of the study was to assess the relationships between concentrations of toll-like receptors in inflammatory granulation tissue within periodontal pocket and atheroma of the same patient. Methods: The study population consisted of 15 patients with carotid atherosclerosis and periodontosis. Control group comprised 15 patients with periodontosis but
Background and Objectives: The pleiotropic cytokine, interferon-gamma (IFN-gamma), is a key proinflammatory mediator abundantly expressed in atherosclerotic lesions and periodontosis. The aim of this study was to determine relationships between the concentrations of IFN-gamma complexes in inflammatory granulation tissue obtained from periodontal pockets and atheromatous plaques of the same patient, and compare with control specimens. Methods: The study population consisted of 15 patients from the Department of General Surgery selected for carotid endarterectomy. Control group comprised 15 patients whose general condition was good; inflammatory granulation tissue was obtained from osseous defects during open flap curettage. IFNG, IFNGR1 and IFNGR2 expression in inflammatory granulation tissue and atheromatous plaques was analysed using quantitative reverse
poly-vinyl template. The pattern of the layer was a grid pattern with average gaps area of 500 m2 . The contact points of each filament were connected by stamping in the warm water. The multilayer scaffolds were made by depositing of monolayer scaffolds and stamping. The physical properties and biocompatibilities of the scaffolds were evaluated. Methods: The physical properties of the scaffolds including the surface morphologies, mechanical strength and degradation behaviours were assessed. The cellscaffold constructs were made by seeding mouse osteoblast cell lines at 1 × 105 cells/scaffold and cultured for 21 days. MTT and enzyme-linked immunosorbent assay test of alkaline phosphatase and osteocalcin were performed to evaluate the cell proliferation and differentiation respectively. The alizarin red S staining was used to quantify the mineralisation of the constructs. Results: The scaffolds had interconnecting structures, pore size and surface morphology suitable for cell attachment with sufficient strength for using in the oral region. The cells proliferated well during culture period. The increase of alkaline phosphatase activity, osteocalcin level and the degree of mineralisation indicated the well differentiation. Conclusion: The study demonstrated that the scaffolds fabricated by MSMD technique were compatible for bone regeneration. doi:10.1016/j.ijom.2009.03.691
N. Thuaksuban ∗ , T. Nuntanaranont, W. Pattanachot, S. Suttapreyasri, K. Sutin Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Prince of Songkla University, Hatyai, Songkhla, Thailand
Background and Objectives: The novel technique of melt spinning and multilayer deposition (MSMD) was practical and economical for fabricating the biodegradable polycaprolactone (PCL) three-dimensional scaffolds. In brief, the PCL monofilaments were fabricated by melting and extruding through custom made extruder followed by stretching. The filaments were arranged to form the monolayer scaffolds on the custom made
without history of any systemic disease. TLRs expression in inflammatory granulation tissue and atheromatous plaques was analysed using quantitative reverse transcriptase-polymerase chain reaction profiling. Results: TLR3 and TLR 7 in granulation tissue showed a relatively high differentiation—TLR3 variance reached 1.1, and TLR7 variance was 0.95 (standard deviations were 1.049 and 0.97, respectively), so in case of these two TLRs neither median nor mean values should be considered reliable. The mean mRNA copy number of TLR1 is higher in granulation tissue than in atheromatous plaque whereas TLR2, TLR6 and TLR7 readings in granulation tissue are noticeably lower than those in atheromata. In the case of TLR3, TL4, TLR5 and TLR8, granular tissue and atheroma readings are practically equal. Conclusion: TLR2 seems to be the most sensitive marker of the relationship between carotid atherosclerosis and periodontal disease. doi:10.1016/j.ijom.2009.03.692
P220 The role of interferon-gamma in the aetiology of atheroma and periodontitis I. Niedzielska 1,∗ , M. Tkacz 2 Department of Craniomaxillofacial Surgery, Silesian Medical University, Katowice, Poland 2 Institute of Computer Science, University of Silesia, Poland
1
doi:10.1016/j.ijom.2009.03.690
P218 The biodegradable polycaprolactone threedimensional scaffold fabricated by melt spinning and multilayer deposition for bone tissue engineering
595
P219 The role of toll-like receptors in carotid atherosclerosis and periodontal disease I. Niedzielska 1,∗ , M. Tkacz 2 , H. Borgiel-Marek 1 1 Department of Craniomaxillofacial Surgery, Silesian Medical University, Katowice, Poland 2 Institute of Computer Science, University of Silesia, Poland
Background and Objectives: The role of toll-like receptors (TLRs) in atherosclerosis and periodontosis has been investigated but is not clear. Aim of the study was to assess the relationships between concentrations of toll-like receptors in inflammatory granulation tissue within periodontal pocket and atheroma of the same patient. Methods: The study population consisted of 15 patients with carotid atherosclerosis and periodontosis. Control group comprised 15 patients with periodontosis but
Background and Objectives: The pleiotropic cytokine, interferon-gamma (IFN-gamma), is a key proinflammatory mediator abundantly expressed in atherosclerotic lesions and periodontosis. The aim of this study was to determine relationships between the concentrations of IFN-gamma complexes in inflammatory granulation tissue obtained from periodontal pockets and atheromatous plaques of the same patient, and compare with control specimens. Methods: The study population consisted of 15 patients from the Department of General Surgery selected for carotid endarterectomy. Control group comprised 15 patients whose general condition was good; inflammatory granulation tissue was obtained from osseous defects during open flap curettage. IFNG, IFNGR1 and IFNGR2 expression in inflammatory granulation tissue and atheromatous plaques was analysed using quantitative reverse