- Email: [email protected]
The correction of the hormonal insufficiency of the ovaries with the transplantation of the ovarian tissue
144
THURSDAY,
trimester appears to offer the most optimal time for fetal cell recovery from maternal blood for the purpose of prenatal diagnosis.
P4.16.18 SOMATIC MUTATIONS AND GENETIC POLYMORPHISMS OF THE PPPRlR3 GENE IN PATIENTS WITH SEVERAL TYPES OF CANCERS S. Takakura (11, A. Okamoto (2), .I. Yokota (l), T. Tanaka (2), T. Kohno
(l), T. Yamada (l), K. Shimizu (l), S. Ohwada (l), (1)National Cancer Center Research Institute, l-l, Tsukiji 5-chome, Chuo-ku, Tokyo, Japan, 1040045, (2) Jikei University School of Medicine, Tokyo, Japan. Objective: Recent studies on mEN and PPP2RlB (a regulatory subunit of PPZA) mutations in human cancer indicate that aberrations of intracellular signaling pathways via protein phosphatases (PP) are involved in human carcinogenesis. We examined genetic alterations of the PPPlR3 gene located at chromosome 7q31, which encodes the regulatory subunit 3 of PPl, in various types of human cancers to asses the role of this gene in human carcinogenesis. Study Methods: All of coding exons of PPPlR3 were examined for mutations and polymorphisms in 104 cancer cell lines and 192 primary tumors by PCR-SSCP and direct sequencing. Results: Sixteen mutations of the PPPlR3 gene were detected in 9 of 104 cancer cell lines and 5 of 192 primary tumors, and they occurred in a subset of ovarian cancer, lung cancer, colorectal cancer, and gastric cancer. Sixteen mutations detected consisted of 3 nonsense mutations, 10 missense mutations, 2 silent mutations and a mutation in intron sequence. Seven novel single nucleotide polymorphisms (SNPs) associated with the substitution of amino acids were also identified in cancer patients, in addition to three known nonsynonymous SNPs, including three previously reported ones as having an impact on the susceptibility to insulin resistant disorders. Conclusions: Differences in the activities and properties of multiple PPPlR3 proteins, which are produced in human cells due to variable somatic mutations and genetic polymorphisms in the PPPlR3 gene, can be involved in human carcinogenes
P4.16.19 f3,-GLYCOPROTEIN AND IMMUNE PLACENTAL TISSUES OF WOMEN PREGNANCY LOSS
COMPLEXES LOCATION WITH REPEATED
G.L.Gromiko. L.B.Zubjitskya, I.P.Pavlov Medical Univercity, D.O.Ott Institute of Obstetrics and Gynecology, St.Petersburg,
IN
Russia
Objectives: To study the consequence of antiphospholipid antibodies (aPL) on pregnancy complications we investigate localization of flzglycoprotein I @,-GP I) in placental tissues of women with recurrent pregnancy loss. Having a regulatory role in blood coagulation, fl,-GP I appear significantly related to most frequent complications (thrombosis and fetal loss) in patients with aPL. Study Methods: 46 samples of placentae and blood sera of women with repeated pregnancy losses were investigated. Method of fluorescent antibodies in indirect modification with sera of women that were tested on the presence of anti-cardiolipin antibodies on bovine heart was used. For elimination of cross-reacted antibodies to bovine cardiolipin and save antibodies to fl,-GP I tested sera were adsorbed by standart cardiolipin antigen. Placentae cryostat sections of women were treated for detecting complement-fixing immune complexes (IC). In parallel after washing, placentae sections were treated by adsorbede sera containing fl,-GP I. Results: Specific luminescence of IC was detected in 91% of women, aPL in 78%. Luminescence was detected on membrans of syncytiotrophoblast and on endothelium of chorion vessels. The topographical identity of a luminescence of complement-fixing IC is established with sites of a luminescences, obtained at handling washing placentae sections by adsorbede sera. Conclusions: We assume presence of the fl,-GP I in a structure of IC localized in a placental tissue. Being an antigenic target of aPL, fl,-GP I can be immediately connected to development of placental thrombosis
SEPTEMBER
P4.16.20 THE APPLICATION GENNO-ENGINEERING azp- INTERFERON THE TREATMENT NONSPECIFIC INFLAMMATORY DISEASES OF WOMEN’S REPRODUCTION ORGANS T.V. Bannvcova (l), T.N. Dyomina (2), G.D. Mysuna (2),
(1) (2)
7
IN
Donetsk State Regional Centre Gruarding Maternity and Childhood, Donetsk, Panfilova, Ukraine. Donetsk State Medical University, Done&k, Illycha, Ukraine.
Objectives: The aim of given research was the study of the effect gennoengineering ~1~~ interferon during treatment nonspecific inflammatory diseases of women’s internal genitals. Study Methods: It was conducted clinic-immunology investigation 108 women with nonspecific inflammatory diseases of ovaries and of uterine appendages. The 70 patients had immunocorrection therapy by “Laferon” (preparation ~1~~ interferon), the 40 patients -had the traditional antibacterial treatment. Results: The suppresion cell and humoral links of the immunity (the deficit T-cell, the dysimmunoglobulynemia), the factors of the nonspecific stability (the reduction of metabolic potence leukocytes) the interferon-deficit state (the higher level serum interferon’s, the reduction of the production the direct CIand y interferon) was revealed in patients of two groups. It was marked the normalization of functions the immunocompetents system’s in the group, who was treated by the “Laferon.” The convalescence was coming in 97.1% of the patients, it was marked improvement of state considerable 2.9% of patients. The positive dynamics of the index immunity wasn’t marked in the patient, who had antibacterial therapy: 72.5% women has convalescence; the state has improved in 20%; the therapy was not effective for 7.5% of the patients. Conclusions: The using a preparation ~1~~ interferon to allow back to normalcy the functions of the immunocompetent systems’ and to raise the effectiveness therapy nonspecific inflammatory diseases of internal genitals females.
P4.16.21 THE CORRECTION OF THE HORMONAL INSUFFICIENCY OF THE OVARIES WITH THE TRANSPLANTATION OF THE OVARIAN TISSUE L. Dept. PED/AD GYN, Done&k regional center of Maternity
and Child Protection, Done&k, Ukraine. Objective: The task of our invention is the correction of the hormonal insufficiency of the ovaries by the transplantation of the culture of the ovarian tissue cells. Study Methods: A new thing of this method is that the culture of the cells of the ovarian tissue is used as a transplant. The safety and the functional ability of the transplant is a main condition of the successful hormonal correction of the sufficient function of the ovaries. Results: The cells of the fetus ovarian tissue, which do not have an antigen activity, have not undergone the rejection, function on being introduced into the organism, producing estradiol and progesterone, in connection with this it appeared a possibility with the help of allotransplantation to correct the insufficiency of the ovarian function. Effective functioning transplanted culture of the ovarian tissue and absorption of estrogens, produced by it, is possible due to the increased adequate and sufficient permeability through the walls newly formed capsule. Conclusions: Thus, the quality of the introduced material was improved, the permeability throng the formed capsule was increased, the duration of the functioning of the transplant cells was increased, the culture in the form of suspension was introduced and activation of blood supply round the formed capsule, contributing to the formation and appearance of estrogens, lead to the start of the mechanisms of H-H-O regulation and initiation of extragonadal synthesis of estrogens and progesterone.
P4.16.22 THE ROLE OF AMNIOTIC FLUID INTERPHASE FISH ANALYSIS IN PATIENT MANAGEMENT W.C. Leung (1,2), E.J.T. Winsor (l), G. Seaward (l), R. Windrim (l),
D. Chitayaat (l), G. Ryan (1) (1) University of Toronto Perinatal Complex, Toronto, Ontario, Canada. (2) Dept. OBIGYN, University of Hong Kong, Hong Kong, China.
THURSDAY,
trimester appears to offer the most optimal time for fetal cell recovery from maternal blood for the purpose of prenatal diagnosis.
P4.16.18 SOMATIC MUTATIONS AND GENETIC POLYMORPHISMS OF THE PPPRlR3 GENE IN PATIENTS WITH SEVERAL TYPES OF CANCERS S. Takakura (11, A. Okamoto (2), .I. Yokota (l), T. Tanaka (2), T. Kohno
(l), T. Yamada (l), K. Shimizu (l), S. Ohwada (l), (1)National Cancer Center Research Institute, l-l, Tsukiji 5-chome, Chuo-ku, Tokyo, Japan, 1040045, (2) Jikei University School of Medicine, Tokyo, Japan. Objective: Recent studies on mEN and PPP2RlB (a regulatory subunit of PPZA) mutations in human cancer indicate that aberrations of intracellular signaling pathways via protein phosphatases (PP) are involved in human carcinogenesis. We examined genetic alterations of the PPPlR3 gene located at chromosome 7q31, which encodes the regulatory subunit 3 of PPl, in various types of human cancers to asses the role of this gene in human carcinogenesis. Study Methods: All of coding exons of PPPlR3 were examined for mutations and polymorphisms in 104 cancer cell lines and 192 primary tumors by PCR-SSCP and direct sequencing. Results: Sixteen mutations of the PPPlR3 gene were detected in 9 of 104 cancer cell lines and 5 of 192 primary tumors, and they occurred in a subset of ovarian cancer, lung cancer, colorectal cancer, and gastric cancer. Sixteen mutations detected consisted of 3 nonsense mutations, 10 missense mutations, 2 silent mutations and a mutation in intron sequence. Seven novel single nucleotide polymorphisms (SNPs) associated with the substitution of amino acids were also identified in cancer patients, in addition to three known nonsynonymous SNPs, including three previously reported ones as having an impact on the susceptibility to insulin resistant disorders. Conclusions: Differences in the activities and properties of multiple PPPlR3 proteins, which are produced in human cells due to variable somatic mutations and genetic polymorphisms in the PPPlR3 gene, can be involved in human carcinogenes
P4.16.19 f3,-GLYCOPROTEIN AND IMMUNE PLACENTAL TISSUES OF WOMEN PREGNANCY LOSS
COMPLEXES LOCATION WITH REPEATED
G.L.Gromiko. L.B.Zubjitskya, I.P.Pavlov Medical Univercity, D.O.Ott Institute of Obstetrics and Gynecology, St.Petersburg,
IN
Russia
Objectives: To study the consequence of antiphospholipid antibodies (aPL) on pregnancy complications we investigate localization of flzglycoprotein I @,-GP I) in placental tissues of women with recurrent pregnancy loss. Having a regulatory role in blood coagulation, fl,-GP I appear significantly related to most frequent complications (thrombosis and fetal loss) in patients with aPL. Study Methods: 46 samples of placentae and blood sera of women with repeated pregnancy losses were investigated. Method of fluorescent antibodies in indirect modification with sera of women that were tested on the presence of anti-cardiolipin antibodies on bovine heart was used. For elimination of cross-reacted antibodies to bovine cardiolipin and save antibodies to fl,-GP I tested sera were adsorbed by standart cardiolipin antigen. Placentae cryostat sections of women were treated for detecting complement-fixing immune complexes (IC). In parallel after washing, placentae sections were treated by adsorbede sera containing fl,-GP I. Results: Specific luminescence of IC was detected in 91% of women, aPL in 78%. Luminescence was detected on membrans of syncytiotrophoblast and on endothelium of chorion vessels. The topographical identity of a luminescence of complement-fixing IC is established with sites of a luminescences, obtained at handling washing placentae sections by adsorbede sera. Conclusions: We assume presence of the fl,-GP I in a structure of IC localized in a placental tissue. Being an antigenic target of aPL, fl,-GP I can be immediately connected to development of placental thrombosis
SEPTEMBER
P4.16.20 THE APPLICATION GENNO-ENGINEERING azp- INTERFERON THE TREATMENT NONSPECIFIC INFLAMMATORY DISEASES OF WOMEN’S REPRODUCTION ORGANS T.V. Bannvcova (l), T.N. Dyomina (2), G.D. Mysuna (2),
(1) (2)
7
IN
Donetsk State Regional Centre Gruarding Maternity and Childhood, Donetsk, Panfilova, Ukraine. Donetsk State Medical University, Done&k, Illycha, Ukraine.
Objectives: The aim of given research was the study of the effect gennoengineering ~1~~ interferon during treatment nonspecific inflammatory diseases of women’s internal genitals. Study Methods: It was conducted clinic-immunology investigation 108 women with nonspecific inflammatory diseases of ovaries and of uterine appendages. The 70 patients had immunocorrection therapy by “Laferon” (preparation ~1~~ interferon), the 40 patients -had the traditional antibacterial treatment. Results: The suppresion cell and humoral links of the immunity (the deficit T-cell, the dysimmunoglobulynemia), the factors of the nonspecific stability (the reduction of metabolic potence leukocytes) the interferon-deficit state (the higher level serum interferon’s, the reduction of the production the direct CIand y interferon) was revealed in patients of two groups. It was marked the normalization of functions the immunocompetents system’s in the group, who was treated by the “Laferon.” The convalescence was coming in 97.1% of the patients, it was marked improvement of state considerable 2.9% of patients. The positive dynamics of the index immunity wasn’t marked in the patient, who had antibacterial therapy: 72.5% women has convalescence; the state has improved in 20%; the therapy was not effective for 7.5% of the patients. Conclusions: The using a preparation ~1~~ interferon to allow back to normalcy the functions of the immunocompetent systems’ and to raise the effectiveness therapy nonspecific inflammatory diseases of internal genitals females.
P4.16.21 THE CORRECTION OF THE HORMONAL INSUFFICIENCY OF THE OVARIES WITH THE TRANSPLANTATION OF THE OVARIAN TISSUE L. Dept. PED/AD GYN, Done&k regional center of Maternity
and Child Protection, Done&k, Ukraine. Objective: The task of our invention is the correction of the hormonal insufficiency of the ovaries by the transplantation of the culture of the ovarian tissue cells. Study Methods: A new thing of this method is that the culture of the cells of the ovarian tissue is used as a transplant. The safety and the functional ability of the transplant is a main condition of the successful hormonal correction of the sufficient function of the ovaries. Results: The cells of the fetus ovarian tissue, which do not have an antigen activity, have not undergone the rejection, function on being introduced into the organism, producing estradiol and progesterone, in connection with this it appeared a possibility with the help of allotransplantation to correct the insufficiency of the ovarian function. Effective functioning transplanted culture of the ovarian tissue and absorption of estrogens, produced by it, is possible due to the increased adequate and sufficient permeability through the walls newly formed capsule. Conclusions: Thus, the quality of the introduced material was improved, the permeability throng the formed capsule was increased, the duration of the functioning of the transplant cells was increased, the culture in the form of suspension was introduced and activation of blood supply round the formed capsule, contributing to the formation and appearance of estrogens, lead to the start of the mechanisms of H-H-O regulation and initiation of extragonadal synthesis of estrogens and progesterone.
P4.16.22 THE ROLE OF AMNIOTIC FLUID INTERPHASE FISH ANALYSIS IN PATIENT MANAGEMENT W.C. Leung (1,2), E.J.T. Winsor (l), G. Seaward (l), R. Windrim (l),
D. Chitayaat (l), G. Ryan (1) (1) University of Toronto Perinatal Complex, Toronto, Ontario, Canada. (2) Dept. OBIGYN, University of Hong Kong, Hong Kong, China.