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The correlation between polimorphism of CYP7a1 gene and glycated hemoglobin in hypertensive patients with type 2 diabetes mellitus
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Abstracts / Atherosclerosis 263 (2017) e111ee282
patients with cholestasis. The data for other specific types of cholestatic syndromes except for primary biliary cirrhosis is sparse in the literature. The administration of lipid-lowering agents (LLA) in these syndromes is still controversial. Methods: Herein, we present a rare case of a new-onset, rapid, and severe hypercholesterolemia due to cholestatic liver syndrome. Results: A 39-year-old male patient was referred to our outpatient clinic due to severe new-onset of intermittent right hypochondriac pain, jaundice and hypercholesterolemia (total cholesterol of 958 mg/dl, LDL cholesterol of 871 mg/dl). Paraclinical examinations revealed cholestasis due to inflammation and stenosis of the common bile duct. The patient underwent an ERCP, removal of the ductal gallstones and a stenting at the lower part of the common bile duct. After a follow-up period of three months, the patient remained symptom-free without evidence of jaundice recurrence. Liver enzymes decreased remarkably, while there was a subsequent improvement in patient’s lipid profile without administration of LLA (Table 1). Conclusions: This was a rare case of a new-onset, rapid, and extreme hyperlipidemia due to cholestatic liver syndrome. In such cases, treatment of the primary disorder is associated with normalization of the lipid levels. The administrations of LLA should be personalized according to the physician’s and patient’s preferences.
PO470. THE CORRELATION BETWEEN POLIMORPHISM OF CYP7A1 GENE AND GLYCATED HEMOGLOBIN IN HYPERTENSIVE PATIENTS WITH TYPE 2 DIABETES MELLITUS Nadiia Demikhova, Olga Chernatska, Olga Smiianova, Lyudmyla Vynnychenko. Sumy State University, Sumy, Ukraine Aim: Cholesterol 7-alpha-hydroxylase (CYP7A1) is a microsomal cytochrome P450 that catalyzes the first step in bile acid synthesis. Participants with insulin resistance with arterial hypertension (AH) had higher risk of hyperbileacidemia. Aim: determine of interconnection between the glycated hemoglobin (GHb) level and three genotypes of CYP7a1 gene for prevention cardiovascular complications in patients with type 2 diabetes mellitus (DM) and AH. Methods: We examined 130 patients during our clinical trial. Participants were divided in two groups. There are 80 patients with type 2 DM and AH in the 1 group and 50 persons with AH in the 2 group. The middle age are 57±0.23 and 69±0.57 ears old. The method for diagnostic different allele variants is polymerase chain reaction. All data were analyzed with the help of statistical methods (Excel 2007). Results: The genotypes AA, AC and CC are determined in 5 (6.25%), 22 (27.5%), 53 (66.25%) patients respectively in the I group and 20 (40%), 19 (38%), 11 (22%) in the II group. The levels of GHb are 6.0±0.25% and 4.9±0.31%, p<0,01 for AA genotype, 7.3±0.6% and 5.7±0.42%, p<0.05 for AC genotype, 8.2±0.59% and 6.1±0.45%, p<0.01 for CC genotype. Conclusions: CC allele of CYP7a1 gene is more popular for patients with type 2 DM and concomitant AH. CC genotype is associated with higher levels of GHb which is a risk factor of cardiovascular and cerebrovascular events. As a result the combined therapy is necessary for prevention severe complication of DM and AH especially with CC allele of CYP7a1 gene.
PO471. RESVERATROL ATTENUATES INSUIN RESISTANCE AND LEFT VENTRICULAR DIASTOLIC DYSFUNCTION IN HIGH FAT DIET INDUCED OBESITY MODEL
dyslipidemia and improve LV diastolic dysfunction in high fat diet induced obesity model. Methods: 8 week-old male spontaneously hypertensive rats (SHRs) fed with high fat diet (HFD) were randomized to either RES (SHR-HFD-R, n¼8) or obesity control groups (SHR-HFD-C, n¼8). At the age of 20 weeks, all rats underwent hemodynamic and metabolic studies and Doppler echocardiography. After scarify, we measured collagen type I, III and TGF-b1 in the heart. Results: Compared with Wistar-Kyoto rats (WKYs; normal control, n¼8), SHRs fed with HFD developed more intense degree of insulin resistance but RES significantly improved metabolic and hemodynamic parameters. Echocardiogram showed that RES improved LV diastolic dysfunction (SHRHFD-C vs. SHR-HFD-R; E/A ratio: 1.38±0.05 vs. 1.57±0.11, E/E: 26.8±7.7 vs. 17.9±3.6, p<0.05, respectively) but fail to reduce LV hypertrophy and systolic function. RES significantly lowered systolic blood pressure and postprandial serum glucose levels as compared to obesity control. Moreover, RES inhibited phosphorylation of Smad2/3 in obesity rat hearts. In line with these findings, RES significantly reduced myocardial fibrosis (TGF-b/Smad) and collagen production of the myocardium. Conclusions: We found that diet-induced obesity typically showed intense insulin resistance and a constellation of LV diastolic dysfunction, exacerbated myocardial fibrosis. Resveratrol attenuate insulin resistance and improved LV diastolic dysfunction by attenuating myocardial fibrosis.
PO472. SEX-SPECIFIC RELATIONSHIP BETWEEN COTININE-VERIFIED SECONDHAND SMOKE EXPOSURE AND DIABETES IN 85,697 NEVER-SMOKERS Byung Jin Kim1, Jin Min Han1, Jun Hyung Do2, Bum Soo Kim1, Jin Ho Kang1. 1 Div. of Cardiology, Dept. of Internal Medicine, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, South Korea; 2 Dept. of Cardiology, Ilsan Baek Hospital, Inje University, Koyang, South Korea Aim: This study was performed to evaluate sex-specific relationships of cotinine-verified second-hand smoke(SHS) exposure and diabetes in Korean never-smokers. Methods: Among individuals enrolled in Kangbuk Samsung Health Study(KSHS) and Kangbuk Samsung Cohort Study(KSCS), 85,697 self-reported never-smokers(29,660 men; age 36 ± 6.6 years) between 2011 and 2013 who had urinary cotinine measurements were included. Neversmoker was defined from self-reported questionnaire and cotinine-verified SHS was defined as urinary cotinine level above 50 ng/mL. Results: The prevalence of the SHS exposure group was 2.0% in men and 1.6% in women; the prevalence of diabetes was 2.3% in men and 1.1% in women. In men, the prevalence of diabetes was significantly higher in the SHS exposure group than the non-exposure group(4.9% vs. 2.3%, p<0.001) while in women, the SHS exposure group showed a trend toward diabetes(1.8% vs. 1.1%, p¼0.076). Multivariate regression model adjusted for variables with univariate relationships showed that the SHS exposure group significantly increased the OR for diabetes compared with the nonexposed group in women(OR[95% CI], 2.92[1.67, 5.11], p<0.001), but not in men(1.11[0.67, 1.87], p¼0.684). Log-transformed cotinine level also increased the OR for diabetes in women(1.18[1.08, 1.29], p<0.001), but not in men(1.02[0.95, 1.10], p¼0.592). Furthermore, log-transformed cotinine level was associated with increased homeostatic model assessment-insulin resistance only in women(standardized b¼0.029, p¼0.018 for women and standardized b¼0.024, p¼0.173 for men). Conclusions: This large study shows that cotinine-verified SHS group has higher prevalence of diabetes in both sex but only in women SHS exposure is independently associated with diabetes and insulin resistance after adjusting for multiple covariates.
Sang-Hyun Ihm, Seul-Ki Hong, Kiyuk Chang. Departement of Internal medicine, The Catholic University of Korea, Seoul, South Korea Aim: Obesity-induced myocardial fibrosis is associated with left ventricular (LV) diastolic dysfunction and ultimate heart failure. Resveratrol (RES) is a polyphenol that has been reported to be an anti-diabetic, anti-fibrosis and cardio protective effect. We investigated whether Resveratrol (RES) could attenuate insulin resistance including hypertension, diabetes and
PO473. HIGH SERUM PCSK9 IS ASSOCIATED WITH INCREASED RISK OF NEWONSET DIABETES AFTER TRANSPLANTATION IN RENAL TRANSPLANT RECIPIENTS
Abstracts / Atherosclerosis 263 (2017) e111ee282
patients with cholestasis. The data for other specific types of cholestatic syndromes except for primary biliary cirrhosis is sparse in the literature. The administration of lipid-lowering agents (LLA) in these syndromes is still controversial. Methods: Herein, we present a rare case of a new-onset, rapid, and severe hypercholesterolemia due to cholestatic liver syndrome. Results: A 39-year-old male patient was referred to our outpatient clinic due to severe new-onset of intermittent right hypochondriac pain, jaundice and hypercholesterolemia (total cholesterol of 958 mg/dl, LDL cholesterol of 871 mg/dl). Paraclinical examinations revealed cholestasis due to inflammation and stenosis of the common bile duct. The patient underwent an ERCP, removal of the ductal gallstones and a stenting at the lower part of the common bile duct. After a follow-up period of three months, the patient remained symptom-free without evidence of jaundice recurrence. Liver enzymes decreased remarkably, while there was a subsequent improvement in patient’s lipid profile without administration of LLA (Table 1). Conclusions: This was a rare case of a new-onset, rapid, and extreme hyperlipidemia due to cholestatic liver syndrome. In such cases, treatment of the primary disorder is associated with normalization of the lipid levels. The administrations of LLA should be personalized according to the physician’s and patient’s preferences.
PO470. THE CORRELATION BETWEEN POLIMORPHISM OF CYP7A1 GENE AND GLYCATED HEMOGLOBIN IN HYPERTENSIVE PATIENTS WITH TYPE 2 DIABETES MELLITUS Nadiia Demikhova, Olga Chernatska, Olga Smiianova, Lyudmyla Vynnychenko. Sumy State University, Sumy, Ukraine Aim: Cholesterol 7-alpha-hydroxylase (CYP7A1) is a microsomal cytochrome P450 that catalyzes the first step in bile acid synthesis. Participants with insulin resistance with arterial hypertension (AH) had higher risk of hyperbileacidemia. Aim: determine of interconnection between the glycated hemoglobin (GHb) level and three genotypes of CYP7a1 gene for prevention cardiovascular complications in patients with type 2 diabetes mellitus (DM) and AH. Methods: We examined 130 patients during our clinical trial. Participants were divided in two groups. There are 80 patients with type 2 DM and AH in the 1 group and 50 persons with AH in the 2 group. The middle age are 57±0.23 and 69±0.57 ears old. The method for diagnostic different allele variants is polymerase chain reaction. All data were analyzed with the help of statistical methods (Excel 2007). Results: The genotypes AA, AC and CC are determined in 5 (6.25%), 22 (27.5%), 53 (66.25%) patients respectively in the I group and 20 (40%), 19 (38%), 11 (22%) in the II group. The levels of GHb are 6.0±0.25% and 4.9±0.31%, p<0,01 for AA genotype, 7.3±0.6% and 5.7±0.42%, p<0.05 for AC genotype, 8.2±0.59% and 6.1±0.45%, p<0.01 for CC genotype. Conclusions: CC allele of CYP7a1 gene is more popular for patients with type 2 DM and concomitant AH. CC genotype is associated with higher levels of GHb which is a risk factor of cardiovascular and cerebrovascular events. As a result the combined therapy is necessary for prevention severe complication of DM and AH especially with CC allele of CYP7a1 gene.
PO471. RESVERATROL ATTENUATES INSUIN RESISTANCE AND LEFT VENTRICULAR DIASTOLIC DYSFUNCTION IN HIGH FAT DIET INDUCED OBESITY MODEL
dyslipidemia and improve LV diastolic dysfunction in high fat diet induced obesity model. Methods: 8 week-old male spontaneously hypertensive rats (SHRs) fed with high fat diet (HFD) were randomized to either RES (SHR-HFD-R, n¼8) or obesity control groups (SHR-HFD-C, n¼8). At the age of 20 weeks, all rats underwent hemodynamic and metabolic studies and Doppler echocardiography. After scarify, we measured collagen type I, III and TGF-b1 in the heart. Results: Compared with Wistar-Kyoto rats (WKYs; normal control, n¼8), SHRs fed with HFD developed more intense degree of insulin resistance but RES significantly improved metabolic and hemodynamic parameters. Echocardiogram showed that RES improved LV diastolic dysfunction (SHRHFD-C vs. SHR-HFD-R; E/A ratio: 1.38±0.05 vs. 1.57±0.11, E/E: 26.8±7.7 vs. 17.9±3.6, p<0.05, respectively) but fail to reduce LV hypertrophy and systolic function. RES significantly lowered systolic blood pressure and postprandial serum glucose levels as compared to obesity control. Moreover, RES inhibited phosphorylation of Smad2/3 in obesity rat hearts. In line with these findings, RES significantly reduced myocardial fibrosis (TGF-b/Smad) and collagen production of the myocardium. Conclusions: We found that diet-induced obesity typically showed intense insulin resistance and a constellation of LV diastolic dysfunction, exacerbated myocardial fibrosis. Resveratrol attenuate insulin resistance and improved LV diastolic dysfunction by attenuating myocardial fibrosis.
PO472. SEX-SPECIFIC RELATIONSHIP BETWEEN COTININE-VERIFIED SECONDHAND SMOKE EXPOSURE AND DIABETES IN 85,697 NEVER-SMOKERS Byung Jin Kim1, Jin Min Han1, Jun Hyung Do2, Bum Soo Kim1, Jin Ho Kang1. 1 Div. of Cardiology, Dept. of Internal Medicine, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, South Korea; 2 Dept. of Cardiology, Ilsan Baek Hospital, Inje University, Koyang, South Korea Aim: This study was performed to evaluate sex-specific relationships of cotinine-verified second-hand smoke(SHS) exposure and diabetes in Korean never-smokers. Methods: Among individuals enrolled in Kangbuk Samsung Health Study(KSHS) and Kangbuk Samsung Cohort Study(KSCS), 85,697 self-reported never-smokers(29,660 men; age 36 ± 6.6 years) between 2011 and 2013 who had urinary cotinine measurements were included. Neversmoker was defined from self-reported questionnaire and cotinine-verified SHS was defined as urinary cotinine level above 50 ng/mL. Results: The prevalence of the SHS exposure group was 2.0% in men and 1.6% in women; the prevalence of diabetes was 2.3% in men and 1.1% in women. In men, the prevalence of diabetes was significantly higher in the SHS exposure group than the non-exposure group(4.9% vs. 2.3%, p<0.001) while in women, the SHS exposure group showed a trend toward diabetes(1.8% vs. 1.1%, p¼0.076). Multivariate regression model adjusted for variables with univariate relationships showed that the SHS exposure group significantly increased the OR for diabetes compared with the nonexposed group in women(OR[95% CI], 2.92[1.67, 5.11], p<0.001), but not in men(1.11[0.67, 1.87], p¼0.684). Log-transformed cotinine level also increased the OR for diabetes in women(1.18[1.08, 1.29], p<0.001), but not in men(1.02[0.95, 1.10], p¼0.592). Furthermore, log-transformed cotinine level was associated with increased homeostatic model assessment-insulin resistance only in women(standardized b¼0.029, p¼0.018 for women and standardized b¼0.024, p¼0.173 for men). Conclusions: This large study shows that cotinine-verified SHS group has higher prevalence of diabetes in both sex but only in women SHS exposure is independently associated with diabetes and insulin resistance after adjusting for multiple covariates.
Sang-Hyun Ihm, Seul-Ki Hong, Kiyuk Chang. Departement of Internal medicine, The Catholic University of Korea, Seoul, South Korea Aim: Obesity-induced myocardial fibrosis is associated with left ventricular (LV) diastolic dysfunction and ultimate heart failure. Resveratrol (RES) is a polyphenol that has been reported to be an anti-diabetic, anti-fibrosis and cardio protective effect. We investigated whether Resveratrol (RES) could attenuate insulin resistance including hypertension, diabetes and
PO473. HIGH SERUM PCSK9 IS ASSOCIATED WITH INCREASED RISK OF NEWONSET DIABETES AFTER TRANSPLANTATION IN RENAL TRANSPLANT RECIPIENTS