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The cytochrome P4501A1 T6325C polymorphism is associated with the risk of hypertension
110A
POSTERS: Epidemiology/Special Populations
The number of CRF adjusted for age and sex was significantly related to the presence of IMT or CP (p ⬍ 0.001). The presence of two or more CRF was related to IMT (OR: 1.8; CI 95% 1.5-2.3). The presence of two or more CRF was poorly related to the presence of CP (OR: 1.2; CI 95% 0.9-15), while the presence of three or more of this was associated with the detection of CP (OR: 1.4; CI 95%: 1.1 – 1.8). In a multivariate analysis model, age, male sex, hypertension, diabetes and hypercholesterolemia were independently related with the presence of CL, while age, hypertension, and family history for cardiovascular diseases were significantly and independently related to the presence of plaque. Conclusions: Our data show that age and hypertension are the main independent predictors of carotid atherosclerosis in this Mediterranean population. The presence of two or more CRF related with an increased risk of developing IMT, while the presence of three or more CRF is related to increased risk of developing CP. These findings, confirms the importance of considering the global cardiovascular risk of a patient and to encourage primary cardiovascular prevention strategy, even in a Mediterranean population. Key Words: Arterial Hypertension, Cardiovascular Risk Factors, Carotid Lesions
AJH–May 2005–VOL. 18, NO. 5, PART 2
P-289 PHYSICAL EXERCISE DOES NOT HELP REDUCE BLOOD PRESSURE IN VERY ELDERLY POPULATIONS Eyal Leibovitz, Dimitri Manevich, Reuven J Viskoper, Dov Gavish. Internal Medicine “A”, Wolfson Medical Center, Holon, Israel; Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel; The Holland Immigrants’ Senior Home, Hertselia, Israel; The Forum for Cardiovascular Disease Prevention, Tel Aviv, Israel. We studied the effect of physical activity on the blood pressure levels of elderly residents of a senior home. The habitants of the Holland immigrants’ senior home in Hertselia, Israel (155 people, mean age 85⫾ 1, median 85 years) were enrolled. Data was obtained from the medical charts. The participants were considered physically active if they were swimming, walking or doing gymnastics, at least twice a week on a regular basis. In this analysis we included only the “independent” population (90, 58%). All values are given in average⫾S.E. of average. Mean blood pressure was 131⫾2 / 73⫾1 mmHg and the pulse rate was 71⫾1 beats per minute. Fifty-seven participants (63%) were physically active. Mean blood pressure levels, pulse rate, BMI and rates of hypertension, diabetes and IHD were similar between the groups (table 1). When analyzing blood pressure levels according to physical activity and HTN status (figure 1), hypertension did not affect the results. Blood
P-288 THE CYTOCHROME P4501A1 T6325C POLYMORPHISM IS ASSOCIATED WITH THE RISK OF HYPERTENSION Vasco Lanc¸a, Paula Alcaˆntara, Jose´ B Nogueira, Maria J Laires, Manuel Bicho. Laborato´rio de Gene´tica, Faculdade de Medicina de Lisboa, Lisboa, Portugal; Servic¸o de Medicina I, Hospital de Santa Maria, Lisboa, Portugal; Laborato´rio de Bioquı´mica, Faculdade de Motricidade Humana, Lisboa, Portugal. Cytochrome P4501A1 (CYP1A1) catalyses the hydroxylation of estradiol and arachidonic acid (AA) producing vasoactive substances, such as catecholestrogens and eicosanoids. Estrogens and eicosanoids are considered to be involved in the pathophysiology of hypertension. However the role of Cytochrome P4501A1 activity in the pathophysiology of hypertension is unclear. CYP1A1 has been known to express genetic variance. Some of its polymorphisms are associated with changes in Cytochrome P4501A1 activity. The C allele of T6325C and the G allele of A4889G polymorphisms have been associated with elevated cytochrome P4501A1 activity. We hypothesized that CYP1A1 polymorphisms would be associated with the risk of hypertension.The hypothesis was tested through a case control study in 208 control and 75 hypertensive male and female subjects. Diabetes and thyroid disease were exclusion factors. We determined the genotype for three CYP1A1 polymorphisms (T6325C, A4889G, and C4887A), using PCR-RFLP, in our population. We observed that the C allele of the CYP1A1 T6325C polymorphism was associated with hypertension (p⫽0.008, chi-square test). The odds ratio was 2.3 with a 95% confidence interval of 1.3 to 4.1. No association was found between the two other CYP1A1 gene polymorphisms and hypertension. These results implicate the C allele of the CYP1A1 T6325C polymorphism as a risk factor for the development of hypertension and suggest that the activity of cytochrome P4501A1 may play a role in the pathophysiology of this disease. Key Words: Cytochrome p450 1A1, Hypertension, Population Genetics
pressure levels did not vary between the active and non-active participants, when analyzing according to age quartiles (active vs. non-active: under 80.75 years: 127⫾4 / 74⫾2 vs. 128⫾4 / 77⫾2 mmHg; between the ages 80.75 and 83 years: 125⫾4 / 72⫾2 vs. 129⫾3 / 72⫾2. mmHg; between the ages 83 and 88: 133⫾5 / 73⫾2 mmHg vs. 133⫾5 / 76⫾3 mmHg; and over 88 years: 147⫾13 / 71⫾4 vs. 129⫾6/ 70⫾2 mmHg). Physical exercise has no beneficial effect on blood pressure levels in very elderly populations. Participant data according to exercise status Age (years) Systolic BP (mmHg) Diastolic BP (mmHg) Pulse rate (*bpm) BMI (kg/m2) Hypertension (%) Diabetes mellitus (%) IHD (%)
Physical exercise
Leisure
p Value
83 ⫾ 1 131 ⫾ 3 73 ⫾ 1 72 ⫾ 1 26 ⫾ 1 63 14 39
85 ⫾ 1 130 ⫾ 2 74 ⫾ 1 68 ⫾ 2 26 ⫾ 1 67 21 27
0.14 0.78 0.58 0.09 0.99 0.46 0.28 0.20
* bpm - beats per minute
Key Words: Blood Pressure, Elderly, Physical Exercise
P-290 OXYGEN SATURATION BY PULSE OXIMETRY: A GOOD PREDICTOR OF HYPERTENSION IN OLDER MEN Aurelio Leone, Chiara Bellotto, Danilo Giannini, Alberto Balbarini. Cardio-thoracic, University of Pisa, Angiology Branch, Castelnuovo Magra, SP, Italy; Internal Medicine, Emergency Medicine Unit, La Spezia, SP, Italy. Background: Few data yet exist on the relationship between Blood Pressure (BP) and oxygen saturation (OS) easiest to be measured, now-
POSTERS: Epidemiology/Special Populations
The number of CRF adjusted for age and sex was significantly related to the presence of IMT or CP (p ⬍ 0.001). The presence of two or more CRF was related to IMT (OR: 1.8; CI 95% 1.5-2.3). The presence of two or more CRF was poorly related to the presence of CP (OR: 1.2; CI 95% 0.9-15), while the presence of three or more of this was associated with the detection of CP (OR: 1.4; CI 95%: 1.1 – 1.8). In a multivariate analysis model, age, male sex, hypertension, diabetes and hypercholesterolemia were independently related with the presence of CL, while age, hypertension, and family history for cardiovascular diseases were significantly and independently related to the presence of plaque. Conclusions: Our data show that age and hypertension are the main independent predictors of carotid atherosclerosis in this Mediterranean population. The presence of two or more CRF related with an increased risk of developing IMT, while the presence of three or more CRF is related to increased risk of developing CP. These findings, confirms the importance of considering the global cardiovascular risk of a patient and to encourage primary cardiovascular prevention strategy, even in a Mediterranean population. Key Words: Arterial Hypertension, Cardiovascular Risk Factors, Carotid Lesions
AJH–May 2005–VOL. 18, NO. 5, PART 2
P-289 PHYSICAL EXERCISE DOES NOT HELP REDUCE BLOOD PRESSURE IN VERY ELDERLY POPULATIONS Eyal Leibovitz, Dimitri Manevich, Reuven J Viskoper, Dov Gavish. Internal Medicine “A”, Wolfson Medical Center, Holon, Israel; Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel; The Holland Immigrants’ Senior Home, Hertselia, Israel; The Forum for Cardiovascular Disease Prevention, Tel Aviv, Israel. We studied the effect of physical activity on the blood pressure levels of elderly residents of a senior home. The habitants of the Holland immigrants’ senior home in Hertselia, Israel (155 people, mean age 85⫾ 1, median 85 years) were enrolled. Data was obtained from the medical charts. The participants were considered physically active if they were swimming, walking or doing gymnastics, at least twice a week on a regular basis. In this analysis we included only the “independent” population (90, 58%). All values are given in average⫾S.E. of average. Mean blood pressure was 131⫾2 / 73⫾1 mmHg and the pulse rate was 71⫾1 beats per minute. Fifty-seven participants (63%) were physically active. Mean blood pressure levels, pulse rate, BMI and rates of hypertension, diabetes and IHD were similar between the groups (table 1). When analyzing blood pressure levels according to physical activity and HTN status (figure 1), hypertension did not affect the results. Blood
P-288 THE CYTOCHROME P4501A1 T6325C POLYMORPHISM IS ASSOCIATED WITH THE RISK OF HYPERTENSION Vasco Lanc¸a, Paula Alcaˆntara, Jose´ B Nogueira, Maria J Laires, Manuel Bicho. Laborato´rio de Gene´tica, Faculdade de Medicina de Lisboa, Lisboa, Portugal; Servic¸o de Medicina I, Hospital de Santa Maria, Lisboa, Portugal; Laborato´rio de Bioquı´mica, Faculdade de Motricidade Humana, Lisboa, Portugal. Cytochrome P4501A1 (CYP1A1) catalyses the hydroxylation of estradiol and arachidonic acid (AA) producing vasoactive substances, such as catecholestrogens and eicosanoids. Estrogens and eicosanoids are considered to be involved in the pathophysiology of hypertension. However the role of Cytochrome P4501A1 activity in the pathophysiology of hypertension is unclear. CYP1A1 has been known to express genetic variance. Some of its polymorphisms are associated with changes in Cytochrome P4501A1 activity. The C allele of T6325C and the G allele of A4889G polymorphisms have been associated with elevated cytochrome P4501A1 activity. We hypothesized that CYP1A1 polymorphisms would be associated with the risk of hypertension.The hypothesis was tested through a case control study in 208 control and 75 hypertensive male and female subjects. Diabetes and thyroid disease were exclusion factors. We determined the genotype for three CYP1A1 polymorphisms (T6325C, A4889G, and C4887A), using PCR-RFLP, in our population. We observed that the C allele of the CYP1A1 T6325C polymorphism was associated with hypertension (p⫽0.008, chi-square test). The odds ratio was 2.3 with a 95% confidence interval of 1.3 to 4.1. No association was found between the two other CYP1A1 gene polymorphisms and hypertension. These results implicate the C allele of the CYP1A1 T6325C polymorphism as a risk factor for the development of hypertension and suggest that the activity of cytochrome P4501A1 may play a role in the pathophysiology of this disease. Key Words: Cytochrome p450 1A1, Hypertension, Population Genetics
pressure levels did not vary between the active and non-active participants, when analyzing according to age quartiles (active vs. non-active: under 80.75 years: 127⫾4 / 74⫾2 vs. 128⫾4 / 77⫾2 mmHg; between the ages 80.75 and 83 years: 125⫾4 / 72⫾2 vs. 129⫾3 / 72⫾2. mmHg; between the ages 83 and 88: 133⫾5 / 73⫾2 mmHg vs. 133⫾5 / 76⫾3 mmHg; and over 88 years: 147⫾13 / 71⫾4 vs. 129⫾6/ 70⫾2 mmHg). Physical exercise has no beneficial effect on blood pressure levels in very elderly populations. Participant data according to exercise status Age (years) Systolic BP (mmHg) Diastolic BP (mmHg) Pulse rate (*bpm) BMI (kg/m2) Hypertension (%) Diabetes mellitus (%) IHD (%)
Physical exercise
Leisure
p Value
83 ⫾ 1 131 ⫾ 3 73 ⫾ 1 72 ⫾ 1 26 ⫾ 1 63 14 39
85 ⫾ 1 130 ⫾ 2 74 ⫾ 1 68 ⫾ 2 26 ⫾ 1 67 21 27
0.14 0.78 0.58 0.09 0.99 0.46 0.28 0.20
* bpm - beats per minute
Key Words: Blood Pressure, Elderly, Physical Exercise
P-290 OXYGEN SATURATION BY PULSE OXIMETRY: A GOOD PREDICTOR OF HYPERTENSION IN OLDER MEN Aurelio Leone, Chiara Bellotto, Danilo Giannini, Alberto Balbarini. Cardio-thoracic, University of Pisa, Angiology Branch, Castelnuovo Magra, SP, Italy; Internal Medicine, Emergency Medicine Unit, La Spezia, SP, Italy. Background: Few data yet exist on the relationship between Blood Pressure (BP) and oxygen saturation (OS) easiest to be measured, now-