- Email: [email protected]
The effect of dietary antioxidants upon human blood monocyte immune functions in vitro
THE EFFECT OF DIETARY ANTIOXIDANTS UPON HUMAN BLOOD MONOCYTE IMMUNE FUNCTIONS IN VITRO J.R. Powell, M E.J Barratt, A. Hillebrand, T.L Hurst and G Nebe von Caron cell Biology arsi Phy.siology, (Jnilever Research PX, (‘olworth House. Sharnhrook, Beds, MK-II ILQ. lJK
THE EFFECTS OF EPIDERMAL GROWTH FACTOR ADMINISTRATION WAYS ON GLYCOGEN METABOLISM AND BLOOD GLUCOSE LEVELS H. Sayan, B. Gdntil, A. Tiir~lmaz, N. Celebi Department of Physiology, University of Gazi, Turkey and Department of Pharmaceutical Technology, Gazi University Faculty of Pharmacy, Turkey
Dietary antioxidants were examined for functional effects on monocyte inflammatory processes. Human blood cell preparations were diluted in RPM1 1640 medium and cultured in 5% CO2 at 37°C for IS hrs with Sng/ml lipopolysaccharide or I &ml phytohaemagglutinin together with a range of dietary antioxidants At the end of this culture period the supernatants were collected and ILl/3. IL-6 and TNFcr concentrations determined by ELISA (Quantikine, R&D Systems, Europe). Phagocytosis of fluorescently labelled bacteria and the resulting respiratory burst, detected by the reduction of dihydrorhodamine following uptake of unlabelled bacteria, were detected by flow cytometry (Phagotest/‘Bursttest, Orpegen, Pharma, Heidelberg). The results showed that a range of dietary antioxidants inhibited the induced production of IL-lfl, IL-6 and TNFcl in a dose dependant manner The inhibitory levels generally fell within normal physiological plasma concentrations. The antioxidants had no affect upon either bacterial phagocytosis or respiratory burst. These results suggest that although dietary antioxidants can modulate redox-sensitive inflammatory signals they do not compromise innate defensive processes.
In this study was investigated the effects of Epidermal Growth Factor (EGF) administration ways on glycogen metabolism and blood glucose levels in cold-restraint stress rats (4°C for 4 hours). Gastric mucosal injury associated with hepatic dysfunction. Several studies report different effects of EGF on glycogen metabolism in hepatocytes. The liver is a target tissue for EGF. EGF stimulates glycogen degradation in both rat and mouse hepatocytes. We studied the interaction between EGF and cold-restraint stress in liver glycogen metabolism. Animals were divided to 9 groups. The controls were untreated the other group exposed coldrestraint stress and treated with EGF. EGF administered by intraperitoneally (IP) in saline solition and by intragastric (IG) way in microemultion (ME) or in water for 7 days (3Opg/kg/day). The liver glycogen content decreased by oral ME-EGF treated group (2,79&0.93) compared with IP EGF in saline treated group (6,81*1.98) and IG EGF in water treated group (10.99+1,42). In conclusion; our results ways and forms at the indicated that EGF administration treatment of stress ulcers induce 2 opposite effects on glycogen metabolism: 1) Intragastric ME-EGF treatment decreased glycogen content 2) IP-EGF in water or IG-EGF treatment in saline stimulates glycogen deposition in coldrestraint stressed rats.
LIPID PEROXIDATION PROCESSES IN ORGANISM DURING CRUSH SYNDROME R. R. Rukhadze, N. Pavliashvili, V. A. Kipiani Tbilisi State Medical University, Tbilisi, Georgia
CHANGES IN THE LEVELS OF LUNG ANTIOXIDANT DEFENCE AND THE ROLE OF ANTIOXIDANT THERAPY IN MANAGEMENT OF PATIENTS WITH ADULT RESPIRATORY DISTRESS SYNDROME B. Shakh, V. Lapshin, 1. Boldina, T. Corshunova Scientific Research Institute qf Emergency iiid, St Petersburg, RU.Y.YiO
The actuality of research of processes occurring in crush syndrome is undoubtable. We used the method of electron paramagnetic resonance (EPR) for revealing the intensity of lipid peroxidation and its influence on the cellular metabolism in crush syndrome. It is found out, that the EPR signal of oxidised ceruloplasmin increases, the EPR signal of Fe3’- transferrinn decreases in comparison with control indices in the blood of rats during crush syndrome. At the same time, the EPR signals of methemoglobin and nonhemic iron nitrozilic complexes (Fe%NO) appear in the EPR spectrum of blood. Thus, decrease of antioxidative ability of blood, production of generators of free-radical forms of oxygen (Fe2’, NO), activation of lipid peroxidation, inactivation of adrenoreceptors, hemolysis of etythrocytes, developing of hypoxia occur during crush syndrome. Also it is shown, that increase of intensity of EPR signals of free radicals and iron-sulphurous (FeS) complexes in comparison with control indice occur in the EPR spectrum of liver of rats, this indicates the disturbance of electron transport on NAD.H: ubiquinone-oxidoreductase section in mitochondria of hepatocytes, also EPR signals of MI?+ and MO” containing complexes and nonhemic iron nitrozilic complexes (FeS-NO) increase in comparison with control indices, this points out the inactivation of superoxide dismutase and destruction of membrane structures. Thus, we can conclude, that developing of hypoxia and &hernia of hepatocytes, activation of lipid peroxidation and destruction of mitochondrial electron transport chain occur during crush syndrome. Reversibility of above-mentioned changes depends on duration of exposure.
One of the most frequent causes of death of patients operated on generalised peritonitis is the development of adult respiratory distress syndrome (ARDS). The state of lung’s antioxidant protection (AP) was investigated in 26 patients with ARDS, who were operated on generalised peritonitis. The AP evaluation was real&d by method of arterial and mixed venous blood luminometry. There was comparison group, consists of 1I patients, operated on generalised peritonitis,
but without
ARDS.
We studied
also IO non-surgical patients, as control group. All patients, who had been occurred for ARDS, were found a considerable reduction of lung’s AP that was confirmed by significantly increasing of the
arterial
blood
luminescention
luminescention.
On the contrary,
in groups
ARDS
without
an arterial
blood
was essentially
lower
than the same parameter in a venous blood. The new antioxidant remedy “OLIFEN” (Russia) was administrated in ARDS group. It was injected
intravenously
(i/v) in dose IO mgikg with 7 mgimin
speed of injection. The drug’s administration was accompanied b! considerable reduction of arterial blood luminescention at 19 patients. The coefficient of P,Ol/F,02 was increasing simultaneously. There is no the same effect at 7 patients. The 14 of 19 patients,
who
were
being
noticed a positive effect from when the 7 patients without
“olifen”‘s admission are recovered.
results of therap) died. Conclusions: I. The development of ARDS is being accompanied by reduction of lung’s AP. 2. “Olifen” is an effective drug with antioxidant activity, that is evidence to use it tf ARDS develops.
12
THE EFFECTS OF EPIDERMAL GROWTH FACTOR ADMINISTRATION WAYS ON GLYCOGEN METABOLISM AND BLOOD GLUCOSE LEVELS H. Sayan, B. Gdntil, A. Tiir~lmaz, N. Celebi Department of Physiology, University of Gazi, Turkey and Department of Pharmaceutical Technology, Gazi University Faculty of Pharmacy, Turkey
Dietary antioxidants were examined for functional effects on monocyte inflammatory processes. Human blood cell preparations were diluted in RPM1 1640 medium and cultured in 5% CO2 at 37°C for IS hrs with Sng/ml lipopolysaccharide or I &ml phytohaemagglutinin together with a range of dietary antioxidants At the end of this culture period the supernatants were collected and ILl/3. IL-6 and TNFcr concentrations determined by ELISA (Quantikine, R&D Systems, Europe). Phagocytosis of fluorescently labelled bacteria and the resulting respiratory burst, detected by the reduction of dihydrorhodamine following uptake of unlabelled bacteria, were detected by flow cytometry (Phagotest/‘Bursttest, Orpegen, Pharma, Heidelberg). The results showed that a range of dietary antioxidants inhibited the induced production of IL-lfl, IL-6 and TNFcl in a dose dependant manner The inhibitory levels generally fell within normal physiological plasma concentrations. The antioxidants had no affect upon either bacterial phagocytosis or respiratory burst. These results suggest that although dietary antioxidants can modulate redox-sensitive inflammatory signals they do not compromise innate defensive processes.
In this study was investigated the effects of Epidermal Growth Factor (EGF) administration ways on glycogen metabolism and blood glucose levels in cold-restraint stress rats (4°C for 4 hours). Gastric mucosal injury associated with hepatic dysfunction. Several studies report different effects of EGF on glycogen metabolism in hepatocytes. The liver is a target tissue for EGF. EGF stimulates glycogen degradation in both rat and mouse hepatocytes. We studied the interaction between EGF and cold-restraint stress in liver glycogen metabolism. Animals were divided to 9 groups. The controls were untreated the other group exposed coldrestraint stress and treated with EGF. EGF administered by intraperitoneally (IP) in saline solition and by intragastric (IG) way in microemultion (ME) or in water for 7 days (3Opg/kg/day). The liver glycogen content decreased by oral ME-EGF treated group (2,79&0.93) compared with IP EGF in saline treated group (6,81*1.98) and IG EGF in water treated group (10.99+1,42). In conclusion; our results ways and forms at the indicated that EGF administration treatment of stress ulcers induce 2 opposite effects on glycogen metabolism: 1) Intragastric ME-EGF treatment decreased glycogen content 2) IP-EGF in water or IG-EGF treatment in saline stimulates glycogen deposition in coldrestraint stressed rats.
LIPID PEROXIDATION PROCESSES IN ORGANISM DURING CRUSH SYNDROME R. R. Rukhadze, N. Pavliashvili, V. A. Kipiani Tbilisi State Medical University, Tbilisi, Georgia
CHANGES IN THE LEVELS OF LUNG ANTIOXIDANT DEFENCE AND THE ROLE OF ANTIOXIDANT THERAPY IN MANAGEMENT OF PATIENTS WITH ADULT RESPIRATORY DISTRESS SYNDROME B. Shakh, V. Lapshin, 1. Boldina, T. Corshunova Scientific Research Institute qf Emergency iiid, St Petersburg, RU.Y.YiO
The actuality of research of processes occurring in crush syndrome is undoubtable. We used the method of electron paramagnetic resonance (EPR) for revealing the intensity of lipid peroxidation and its influence on the cellular metabolism in crush syndrome. It is found out, that the EPR signal of oxidised ceruloplasmin increases, the EPR signal of Fe3’- transferrinn decreases in comparison with control indices in the blood of rats during crush syndrome. At the same time, the EPR signals of methemoglobin and nonhemic iron nitrozilic complexes (Fe%NO) appear in the EPR spectrum of blood. Thus, decrease of antioxidative ability of blood, production of generators of free-radical forms of oxygen (Fe2’, NO), activation of lipid peroxidation, inactivation of adrenoreceptors, hemolysis of etythrocytes, developing of hypoxia occur during crush syndrome. Also it is shown, that increase of intensity of EPR signals of free radicals and iron-sulphurous (FeS) complexes in comparison with control indice occur in the EPR spectrum of liver of rats, this indicates the disturbance of electron transport on NAD.H: ubiquinone-oxidoreductase section in mitochondria of hepatocytes, also EPR signals of MI?+ and MO” containing complexes and nonhemic iron nitrozilic complexes (FeS-NO) increase in comparison with control indices, this points out the inactivation of superoxide dismutase and destruction of membrane structures. Thus, we can conclude, that developing of hypoxia and &hernia of hepatocytes, activation of lipid peroxidation and destruction of mitochondrial electron transport chain occur during crush syndrome. Reversibility of above-mentioned changes depends on duration of exposure.
One of the most frequent causes of death of patients operated on generalised peritonitis is the development of adult respiratory distress syndrome (ARDS). The state of lung’s antioxidant protection (AP) was investigated in 26 patients with ARDS, who were operated on generalised peritonitis. The AP evaluation was real&d by method of arterial and mixed venous blood luminometry. There was comparison group, consists of 1I patients, operated on generalised peritonitis,
but without
ARDS.
We studied
also IO non-surgical patients, as control group. All patients, who had been occurred for ARDS, were found a considerable reduction of lung’s AP that was confirmed by significantly increasing of the
arterial
blood
luminescention
luminescention.
On the contrary,
in groups
ARDS
without
an arterial
blood
was essentially
lower
than the same parameter in a venous blood. The new antioxidant remedy “OLIFEN” (Russia) was administrated in ARDS group. It was injected
intravenously
(i/v) in dose IO mgikg with 7 mgimin
speed of injection. The drug’s administration was accompanied b! considerable reduction of arterial blood luminescention at 19 patients. The coefficient of P,Ol/F,02 was increasing simultaneously. There is no the same effect at 7 patients. The 14 of 19 patients,
who
were
being
noticed a positive effect from when the 7 patients without
“olifen”‘s admission are recovered.
results of therap) died. Conclusions: I. The development of ARDS is being accompanied by reduction of lung’s AP. 2. “Olifen” is an effective drug with antioxidant activity, that is evidence to use it tf ARDS develops.
12