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The effect of DNA repair inhibitors on the frequency of DNA breaks
The aim of study was tn investdate relationsbetwenn childran’scog nitivnIunctions and behaviorin eonnt~tin wi*l detected blood had Iewl. WesekctedJlchiklmn. aged Q-loysarsfrom Eratislava.tiich is indu?rtrial city with heary traffic. Blood lead (PbB) kwsl wa dstwtcd by AAS method. Children w&s inwst@stad by nsumbehaviorrl tests battery(psychcmotqr,attention. short tarm msmoryrnd intellect abif. itiesb Individual health and social history of children was oriented on prenatal and wstwtald~lopment. on behaviorand anqualityol Iwn
ad&n&red one &ery 48 h. This tesultad in progressiveincr0a.w in spoManeoub SWD activity in sucwrsiw pwtinieetion petids. while tho convulsive.Pc-inducedactivity.decreased.The obs& changes suppest the developmentof tolerance to the convulsiveelfects of PC and kindling or releasing SW0 from inhibitow effects nf convulsiw activfr/ To check the validrw of that assumption Exrwimem II ~89 performed. PC a doses ot inducing canvutSiveactitiV. #.a 75D.oW; 5caow and 25o.wa IUlkg was appw to 5BparateIlrows of rats ten times every 24 h. For b&r eval&3n of ehangas in the central “era
crsasbd by Friendvirus[I .I]. ToxoplasmaGondii 131or Lagicnel&apneumophila infection14).A quest& arose tx+WIor these findings can be generalizedto Gthar drugs during infeciions related & AIOS. Themfore. kgihphtyof some other drug5 durinQ Frmndvrms or Toxoplasma Gondii infection was inwetigatsd. Pentazoeine end pethldine &fhality incontra~t,tIomorphin~wemnQchan~eddutinQFiiendvirYs infection vnDBA-2 mice. On the c&r hand, an acetychatin dstemse mhtitor nsostiQminwas significant3 more toxic in DE.42 mice infected with Friendvirusaa wstler inthefirst tilial generalionof BALSleBIOAmice infected with 8 non lethal dose of ToxoplasmsGondii. Thesr ~b?iuHs suggest that Friendvirus or ToxoplasmaGandii inkctions Irelating to AIDS] may influenw toxicitqof maw lbul notalll druQ3.
be confirmedforintravenousrppliiatbn of o!i~o&r brnakdawn pmdtlcts. but nor ‘or pectin 111.Tile experilnsnt pressntod ham WY* performed to clear tlta effect of orally adminialeted pectin an the incorporation and elimination of lead. #dterMsnd Mefkcds: Young male AVNflpcV WistsrlAchbtitcke tats rsec&d a pectin rich dist and bw dazes of terd (2tXJ#g PMnL drlnkinfwaterl in a SW& ewmimant.
tion Pwbds was oberwd. In all rats. aft= (he series of PC injectbns. the respmzo to PTZ (increasein SWD aclivity)was mduced. ?hus. the results of the ExrrerimemII suatxestM) tiwmenl of the lolerancd to PC. The obtained data fromboth experime& show that repeated PC inieclions influence the cwwulsiv? and nonconv&ive advifies in oo&d was. There still mmaim a auestion if them is anv connff tion between the decline in reaction to PTZ. which suggest~achangs in CNS sxcitabilii. and the increase in SWD acQd!y Kwnxds:
penicillin: neurotoxicity;seizures
LsUmlky of Morphh, Psntuocins, Pothfdlna qd C4ocatlpmin In hffcs Infutod wkh Fdsnd Virus ov Toxoplastns GmKlll
It hbs bean shown lhat lethality of morphine and THC has besn irv
+.
LBborato,yof UeveEop.Toaicalogy~ hstdute d En/x Medicine Aead SCLCR. Olslrice v Odick@h ho&h. Czech Rwubk Adducts indueeclin tha ONA by chemical mutagens may be alkaliI. ~bile. or may ba mmartsd to DNA bmakp bq the actnonof DNA re pairen~s(sndllnucleases). Whentbe repairof DNA breaks is inhib ited in its polymerirstbn step bv t-~-0_aratinafcranosylCyrosi~~~r~Cl and hydraxywaa (HUi. the accumulation of DNA is obsetved in cells treated with mutagens. ii :!e step pacadmg the incision by the ew cbnuclease is inhibited by novobiocin. the dmcant of DNA breaks is
hlywwds:
cwamic libfes; in vifro: WAR
ad&n&red one &ery 48 h. This tesultad in progressiveincr0a.w in spoManeoub SWD activity in sucwrsiw pwtinieetion petids. while tho convulsive.Pc-inducedactivity.decreased.The obs& changes suppest the developmentof tolerance to the convulsiveelfects of PC and kindling or releasing SW0 from inhibitow effects nf convulsiw activfr/ To check the validrw of that assumption Exrwimem II ~89 performed. PC a doses ot inducing canvutSiveactitiV. #.a 75D.oW; 5caow and 25o.wa IUlkg was appw to 5BparateIlrows of rats ten times every 24 h. For b&r eval&3n of ehangas in the central “era
crsasbd by Friendvirus[I .I]. ToxoplasmaGondii 131or Lagicnel&apneumophila infection14).A quest& arose tx+WIor these findings can be generalizedto Gthar drugs during infeciions related & AIOS. Themfore. kgihphtyof some other drug5 durinQ Frmndvrms or Toxoplasma Gondii infection was inwetigatsd. Pentazoeine end pethldine &fhality incontra~t,tIomorphin~wemnQchan~eddutinQFiiendvirYs infection vnDBA-2 mice. On the c&r hand, an acetychatin dstemse mhtitor nsostiQminwas significant3 more toxic in DE.42 mice infected with Friendvirusaa wstler inthefirst tilial generalionof BALSleBIOAmice infected with 8 non lethal dose of ToxoplasmsGondii. Thesr ~b?iuHs suggest that Friendvirus or ToxoplasmaGandii inkctions Irelating to AIDS] may influenw toxicitqof maw lbul notalll druQ3.
be confirmedforintravenousrppliiatbn of o!i~o&r brnakdawn pmdtlcts. but nor ‘or pectin 111.Tile experilnsnt pressntod ham WY* performed to clear tlta effect of orally adminialeted pectin an the incorporation and elimination of lead. #dterMsnd Mefkcds: Young male AVNflpcV WistsrlAchbtitcke tats rsec&d a pectin rich dist and bw dazes of terd (2tXJ#g PMnL drlnkinfwaterl in a SW& ewmimant.
tion Pwbds was oberwd. In all rats. aft= (he series of PC injectbns. the respmzo to PTZ (increasein SWD aclivity)was mduced. ?hus. the results of the ExrrerimemII suatxestM) tiwmenl of the lolerancd to PC. The obtained data fromboth experime& show that repeated PC inieclions influence the cwwulsiv? and nonconv&ive advifies in oo&d was. There still mmaim a auestion if them is anv connff tion between the decline in reaction to PTZ. which suggest~achangs in CNS sxcitabilii. and the increase in SWD acQd!y Kwnxds:
penicillin: neurotoxicity;seizures
LsUmlky of Morphh, Psntuocins, Pothfdlna qd C4ocatlpmin In hffcs Infutod wkh Fdsnd Virus ov Toxoplastns GmKlll
It hbs bean shown lhat lethality of morphine and THC has besn irv
+.
LBborato,yof UeveEop.Toaicalogy~ hstdute d En/x Medicine Aead SCLCR. Olslrice v Odick@h ho&h. Czech Rwubk Adducts indueeclin tha ONA by chemical mutagens may be alkaliI. ~bile. or may ba mmartsd to DNA bmakp bq the actnonof DNA re pairen~s(sndllnucleases). Whentbe repairof DNA breaks is inhib ited in its polymerirstbn step bv t-~-0_aratinafcranosylCyrosi~~~r~Cl and hydraxywaa (HUi. the accumulation of DNA is obsetved in cells treated with mutagens. ii :!e step pacadmg the incision by the ew cbnuclease is inhibited by novobiocin. the dmcant of DNA breaks is
hlywwds:
cwamic libfes; in vifro: WAR