- Email: [email protected]
The effect of egg yolk medium on human sperm binding in the hemizona assay
208
Citations from the Literature
The effect of egg yolk medium on human sperm hiing hemizona assay Lanzendorf SE; Holmgren WJ; Jeyendran RS
in the
Prentice Women’s Hospital, 333 East Superior Street, Chicago, IL 60611, USA
FERTIL STERIL 1992 58/3 (547-550) Objective: To evaluate, using the hemizona assay (HZA), whether egg yolk treatment of human sperm enhances binding to the human zona pellucida in vitro and to determine whether such a treatment is as efficient as the standard swim-up procedure for promoting sperm binding ability. Design: Ejaculates were divided into aliquots and half incubated at 37°C for 21 h in standard culture medium or combined with buffered medium containing chicken egg yolk and stored at 4°C for 21 h. A second set of ejaculates were split and processed by a standard l-h rise alone or treated with egg yolk medium. Setting: University teaching hospital. Participants: Two healthy sperm donors. Main Outcome Measure: The number of sperm tightly bound to the hemizona were measured and compared between the groups. Results: A significant increase (P 0.0001) in hemizona binding (n = 46) for egg yolk treatment (90.1 f 9.8; range 7-258) as compared with standard culture medium (53.0 f 8.8; range O-228) was observed. Similarly, a significant increase (P 0.0001) in binding (n = 37) for egg yolk treatment (74.9 f 8.2; range 7-219) as compared with samples obtained with a sperm rise (37.1 f 5.7; range 2-122). Conclusions: Treatment of human spermatozoa with an egg yolk medium at 4°C overnight significantly increases sperm binding in the HZA. Tests of human sperm function and fertilization in vitro De Yi Liu; Baker HWG Department of Obstetrics/Gynaecology, University of Melbourne. Royal Women’s Hospital, Melbourne. Vie. 3053, A US
FERTIL STERIL 1992 58/3 (465-483) Objective: To review recent studies on the development of new tests of human sperm function and evaluation of which sperm characteristics are most important for fertilization in vitro by logistic regression analysis. Study Selection: Recent studies on the relationship between putative and new tests of human sperm function and fertility in vitro or in vivo are discussed in this review. Some physiological and technical aspects are included. Main Outcome Measures: Fertilization rates in vitro and sperm tests including standard semen analysis, improved morphology assessment, objective assessment of sperm motility and movement characteristics, nuclear maturity, hypoosmotic swelling, the acrosome and the acrosome reaction, acrosin activity, human sperm-hamster oocyte penetration assay, and sperm zona pellucida (ZP) and sperm-oolemma binding. Results: The percentages of sperm with normal morphology and a normal intact acrosome, mean linearity, and the number of sperm binding to the ZP were highly significantly related to fertilization rates in vitro. Other sperm tests evaluated usually provided no additional information about fertilization rates. The human ZP is highly selective for binding of morphologically normal sperm. Acrosome-reacted human sperm have little or no ability to bind to the ZP. Conclusion: Int J Gynecol Obstet 41
Results of in vitro fertilization can be used to evaluate tests of human sperm function. Logistic regression analysis is a powerful method for determining which groups of sperm characteristics are independently related to fertilization rates. Normal morphology, linearity, acrosome status, and sperm-ZP binding are the most important characteristics for fertilization in vitro. Failure of intiauterbe inseminationin male immmdogicaI iofertility in cases in which all spermatozoa are antibody*oated Francavilla F; Roman0 R; Santucci R; Marrone V, Corrao G Dipartimento di Medicina Interna, Cattedra di Fisiopatologia Endocrina. Vniversita de L’Aquila, 67100 L;Qquila, ITA
FERTIL STERIL 1992 5813 (587-592) Objective: To determine if the overcoming of the cervical mucus barrier removes the interference of sperm-bound antibodies with fertility. Design: Prospective case series. Setting: University-based intrauterine insemination (IUI) homologous program. Patients: Nineteen patients with all spermatozoa in the ejaculate coated by antisperm antibodies. As control group, 86 consecutive patients without antisperm antibodies, treated for oligoasthenozoospermia or mucus hostility. Interventions: Intrauterine inseminations (at least 3 attempts per couple). Main Outcome Measures: The outcome of IUIs, demographic, and seminal parameters were compared between the two groups. Results: No pregnancy occurred in the couples with male immunological infertility, treated by 110 IUIs. Twentythree pregnancies occurred in 22 (25.6%) of the control group couples who were treated by 41 I IUIs. In the group of patients without antisperm antibodies, we demonstrated that the pregnancy rate (PR)/couple in oligoasthenozoospermia without teratozoospermia was similar to that achieved in normozoospermia (35% versus 38.9%), whereas it was significantly affected by teratozoospermia (3.6%). Only three patients with antisperm antibodies had teratozoospermia. Comparing the PR per couple and per cycle between the two groups of patients (with and without antisperm antibodies), excluding the patients with teratozoospermia, significant differences resulted (P 0.005 and P 0.005, respectively). The motile sperm count was not significantly different between the two groups, which also resulted to be homogeneous for demographic data. Moreover, the motile sperm count was not different between the patients with and without antisperm antibodies, who had successful IUI. Conclusions: The analysis of this trial suggests that the failure of IUI in the treatment of male immunological infertility is imputable to antisperm antibodies when they involve all spermatozoa, regardless of semen quality. Effects of ethinyl estradiol on semen quality and various hormonal parameters in a eugonadal male Lubbert H; Leo-Rossberg I; Hammerstein J Klinikum Steglitz der FU Berlin, Hindenburgaizmm 30. D-RUM Berlin 45. DELI
FERTIL STERIL 1992 58/3 (603-608) Objective: To determine the influence of estrogens on male fertility. Design: A 36-year-old eugonadal male was subjected to two different regimens of treatment with ethinyl estradiol
Citations from the Literature
The effect of egg yolk medium on human sperm hiing hemizona assay Lanzendorf SE; Holmgren WJ; Jeyendran RS
in the
Prentice Women’s Hospital, 333 East Superior Street, Chicago, IL 60611, USA
FERTIL STERIL 1992 58/3 (547-550) Objective: To evaluate, using the hemizona assay (HZA), whether egg yolk treatment of human sperm enhances binding to the human zona pellucida in vitro and to determine whether such a treatment is as efficient as the standard swim-up procedure for promoting sperm binding ability. Design: Ejaculates were divided into aliquots and half incubated at 37°C for 21 h in standard culture medium or combined with buffered medium containing chicken egg yolk and stored at 4°C for 21 h. A second set of ejaculates were split and processed by a standard l-h rise alone or treated with egg yolk medium. Setting: University teaching hospital. Participants: Two healthy sperm donors. Main Outcome Measure: The number of sperm tightly bound to the hemizona were measured and compared between the groups. Results: A significant increase (P 0.0001) in hemizona binding (n = 46) for egg yolk treatment (90.1 f 9.8; range 7-258) as compared with standard culture medium (53.0 f 8.8; range O-228) was observed. Similarly, a significant increase (P 0.0001) in binding (n = 37) for egg yolk treatment (74.9 f 8.2; range 7-219) as compared with samples obtained with a sperm rise (37.1 f 5.7; range 2-122). Conclusions: Treatment of human spermatozoa with an egg yolk medium at 4°C overnight significantly increases sperm binding in the HZA. Tests of human sperm function and fertilization in vitro De Yi Liu; Baker HWG Department of Obstetrics/Gynaecology, University of Melbourne. Royal Women’s Hospital, Melbourne. Vie. 3053, A US
FERTIL STERIL 1992 58/3 (465-483) Objective: To review recent studies on the development of new tests of human sperm function and evaluation of which sperm characteristics are most important for fertilization in vitro by logistic regression analysis. Study Selection: Recent studies on the relationship between putative and new tests of human sperm function and fertility in vitro or in vivo are discussed in this review. Some physiological and technical aspects are included. Main Outcome Measures: Fertilization rates in vitro and sperm tests including standard semen analysis, improved morphology assessment, objective assessment of sperm motility and movement characteristics, nuclear maturity, hypoosmotic swelling, the acrosome and the acrosome reaction, acrosin activity, human sperm-hamster oocyte penetration assay, and sperm zona pellucida (ZP) and sperm-oolemma binding. Results: The percentages of sperm with normal morphology and a normal intact acrosome, mean linearity, and the number of sperm binding to the ZP were highly significantly related to fertilization rates in vitro. Other sperm tests evaluated usually provided no additional information about fertilization rates. The human ZP is highly selective for binding of morphologically normal sperm. Acrosome-reacted human sperm have little or no ability to bind to the ZP. Conclusion: Int J Gynecol Obstet 41
Results of in vitro fertilization can be used to evaluate tests of human sperm function. Logistic regression analysis is a powerful method for determining which groups of sperm characteristics are independently related to fertilization rates. Normal morphology, linearity, acrosome status, and sperm-ZP binding are the most important characteristics for fertilization in vitro. Failure of intiauterbe inseminationin male immmdogicaI iofertility in cases in which all spermatozoa are antibody*oated Francavilla F; Roman0 R; Santucci R; Marrone V, Corrao G Dipartimento di Medicina Interna, Cattedra di Fisiopatologia Endocrina. Vniversita de L’Aquila, 67100 L;Qquila, ITA
FERTIL STERIL 1992 5813 (587-592) Objective: To determine if the overcoming of the cervical mucus barrier removes the interference of sperm-bound antibodies with fertility. Design: Prospective case series. Setting: University-based intrauterine insemination (IUI) homologous program. Patients: Nineteen patients with all spermatozoa in the ejaculate coated by antisperm antibodies. As control group, 86 consecutive patients without antisperm antibodies, treated for oligoasthenozoospermia or mucus hostility. Interventions: Intrauterine inseminations (at least 3 attempts per couple). Main Outcome Measures: The outcome of IUIs, demographic, and seminal parameters were compared between the two groups. Results: No pregnancy occurred in the couples with male immunological infertility, treated by 110 IUIs. Twentythree pregnancies occurred in 22 (25.6%) of the control group couples who were treated by 41 I IUIs. In the group of patients without antisperm antibodies, we demonstrated that the pregnancy rate (PR)/couple in oligoasthenozoospermia without teratozoospermia was similar to that achieved in normozoospermia (35% versus 38.9%), whereas it was significantly affected by teratozoospermia (3.6%). Only three patients with antisperm antibodies had teratozoospermia. Comparing the PR per couple and per cycle between the two groups of patients (with and without antisperm antibodies), excluding the patients with teratozoospermia, significant differences resulted (P 0.005 and P 0.005, respectively). The motile sperm count was not significantly different between the two groups, which also resulted to be homogeneous for demographic data. Moreover, the motile sperm count was not different between the patients with and without antisperm antibodies, who had successful IUI. Conclusions: The analysis of this trial suggests that the failure of IUI in the treatment of male immunological infertility is imputable to antisperm antibodies when they involve all spermatozoa, regardless of semen quality. Effects of ethinyl estradiol on semen quality and various hormonal parameters in a eugonadal male Lubbert H; Leo-Rossberg I; Hammerstein J Klinikum Steglitz der FU Berlin, Hindenburgaizmm 30. D-RUM Berlin 45. DELI
FERTIL STERIL 1992 58/3 (603-608) Objective: To determine the influence of estrogens on male fertility. Design: A 36-year-old eugonadal male was subjected to two different regimens of treatment with ethinyl estradiol