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The effect of flavonoids on regenerated hair follicles with pigmentation
Abstracts from the 41st Annual Meeting / Journal of Dermatological Science 86 (2017) e1–e95
Conclusion: This study suggests that BPO gel and adapalene gel showed comedolytic effects on rhino mice via a different mechanism each other. http://dx.doi.org/10.1016/j.jdermsci.2017.02.183 P09-13[O3-06] The effect of flavonoids on regenerated hair follicles with pigmentation Nobuhiko Taguchi 1,2,∗ , Minoru Yuriguchi 2 , Takumi Honma 1 , Toshihiro Hata 1 , Emi Kamiya 1 , Ai Kobayashi 1 , Ryosuke Kitai 2 , Hitomi Aoki 2 , Takahiro Kunisada 2 1 General Research & Development Institute, HoyuCo., Ltd., Japan 2 Department of Tissue and Organ Development, Gifu University Graduate School of Medicine, Japan If the skin of adult mice is removed and if a wide area of the skin is wounded, regeneration of the epidermis and dermis occurs, and several days later, new hair follicles are formed from the epidermis. However, in recent years, melanocyte stem cells were found to move to the epidermis from existing hair follicles near the wound and become epidermal pigment cells. In cases where there is movement to the area of hair follicle regeneration, the regeneration of hair follicles with pigmentation has been confirmed. In our study, we conducted an experiment with the hypothesis that by applying flavonoids onto the wounds of mice, inflammation would be suppressed and therefore there would be a higher ratio of regenerated hair follicles with pigmentation. In addition, the effect of Yerba Santa (Eriodictyon angustifolium and E. californicum) which contains various flavonoids, was also studied. Yerba Santa grows on the western coast of America and has traditionally been used in the treatment of wounds. The results of the experiment showed a significant effect of flavonoids (two OH groups in the B ring) on new hair growth with pigmentation, but no effect of flavonoids (one OH groups in the B ring). In addition, even with E. angustifolium, a new hair regeneration effect with pigmentation was observed, but not with E. californicum. E. angustifolium contains flavonoids (two OH groups in the B ring) levels are higher than those found in E. californicum. In contrast, we did not observe a significant difference in the quantity of flavonoids (one OH groups in the B ring) between E. angustifolium and E. californicum. In this report, we also report not only the effect of regenerated hair but also the effect of hair growth/anti-gray hair.
e63
Category 10 (P10): Immunology 1: Adaptive Immunity P10-01[I-3] Dual aspects of B cells in tumor immunity; B cells are capable of positive and negative regulation for tumor immunity against B16 melanoma Tadahiro Kobayashi 1,∗ , Takashi Matsushita 1 , Yasuhito Hamaguchi 1 , Minoru Hasegawa 2 , Manabu Fujimoto 3 , Kazuhiko Takehara 1 1
Department of Dermatology, Faculty of Medicine, Institute of Medical, Pharmaceutical, and Health Sciences, Kanazawa University, Ishikawa, Japan 2 Dermatology, University of Fukui, Fukui, Japan 3 Dermatology, University of Tsukuba, Tsukuba, Japan B cells are known to be critical mediators of tumor immunity; however, the precise mechanism is remain unclear. In this study, we investigated the role of B cells in the tumor immunity using melanoma mouse model. B-cell linker protein (BLNK) is required for B cell development as evidenced by BLNK-deficient (BLNK−/− ) mice, in which the development and function of B cells are severely impaired. B16F10 melanoma grew more aggressively in BLNK−/− mice as compared to wild-type mice. As expected, tumor-infiltrating B cell numbers were decreased in BLNK−/− mice. Adoptive transfer of wild-type B cells into BLNK−/− mice attenuated B16F10 melanoma growth with increase numbers of B cells and T cells infiltrating into tumors. In addition, percentages of interferon-g and tumor necrosis factor-a producing tumor-infiltrating T cells were restored. These results support the concept that B cells enhance tumor immunity by promoting T cell infiltration into tumors and cytokine production within the tumor microenvironment. On the other hand, an ability of B cells to negatively regulate contact hypersensitivity and experimental autoimmune encephalomyelitis responses is attributed to B-cell provision of IL-10. The B-cell subset that produces IL-10 is identified as regulatory B cells (Bregs). In phosphatase and tensin homolog (PTEN)-deficient mice, Breg subset was significantly expanded and B16F10 melanoma grew more aggressively as compared to control mice. Tumor-infiltrating B cell numbers were significantly increased in B cell-specific PTEN-deficient mice. Interestingly, the tumor-infiltrating B cell consisted mostly of Bregs. These results suggest that Bregs negatively regulate tumor immunity within the tumor microenvironment. Taken together, B cells have dual aspects in tumor immunity.
http://dx.doi.org/10.1016/j.jdermsci.2017.02.184 http://dx.doi.org/10.1016/j.jdermsci.2017.02.185
Conclusion: This study suggests that BPO gel and adapalene gel showed comedolytic effects on rhino mice via a different mechanism each other. http://dx.doi.org/10.1016/j.jdermsci.2017.02.183 P09-13[O3-06] The effect of flavonoids on regenerated hair follicles with pigmentation Nobuhiko Taguchi 1,2,∗ , Minoru Yuriguchi 2 , Takumi Honma 1 , Toshihiro Hata 1 , Emi Kamiya 1 , Ai Kobayashi 1 , Ryosuke Kitai 2 , Hitomi Aoki 2 , Takahiro Kunisada 2 1 General Research & Development Institute, HoyuCo., Ltd., Japan 2 Department of Tissue and Organ Development, Gifu University Graduate School of Medicine, Japan If the skin of adult mice is removed and if a wide area of the skin is wounded, regeneration of the epidermis and dermis occurs, and several days later, new hair follicles are formed from the epidermis. However, in recent years, melanocyte stem cells were found to move to the epidermis from existing hair follicles near the wound and become epidermal pigment cells. In cases where there is movement to the area of hair follicle regeneration, the regeneration of hair follicles with pigmentation has been confirmed. In our study, we conducted an experiment with the hypothesis that by applying flavonoids onto the wounds of mice, inflammation would be suppressed and therefore there would be a higher ratio of regenerated hair follicles with pigmentation. In addition, the effect of Yerba Santa (Eriodictyon angustifolium and E. californicum) which contains various flavonoids, was also studied. Yerba Santa grows on the western coast of America and has traditionally been used in the treatment of wounds. The results of the experiment showed a significant effect of flavonoids (two OH groups in the B ring) on new hair growth with pigmentation, but no effect of flavonoids (one OH groups in the B ring). In addition, even with E. angustifolium, a new hair regeneration effect with pigmentation was observed, but not with E. californicum. E. angustifolium contains flavonoids (two OH groups in the B ring) levels are higher than those found in E. californicum. In contrast, we did not observe a significant difference in the quantity of flavonoids (one OH groups in the B ring) between E. angustifolium and E. californicum. In this report, we also report not only the effect of regenerated hair but also the effect of hair growth/anti-gray hair.
e63
Category 10 (P10): Immunology 1: Adaptive Immunity P10-01[I-3] Dual aspects of B cells in tumor immunity; B cells are capable of positive and negative regulation for tumor immunity against B16 melanoma Tadahiro Kobayashi 1,∗ , Takashi Matsushita 1 , Yasuhito Hamaguchi 1 , Minoru Hasegawa 2 , Manabu Fujimoto 3 , Kazuhiko Takehara 1 1
Department of Dermatology, Faculty of Medicine, Institute of Medical, Pharmaceutical, and Health Sciences, Kanazawa University, Ishikawa, Japan 2 Dermatology, University of Fukui, Fukui, Japan 3 Dermatology, University of Tsukuba, Tsukuba, Japan B cells are known to be critical mediators of tumor immunity; however, the precise mechanism is remain unclear. In this study, we investigated the role of B cells in the tumor immunity using melanoma mouse model. B-cell linker protein (BLNK) is required for B cell development as evidenced by BLNK-deficient (BLNK−/− ) mice, in which the development and function of B cells are severely impaired. B16F10 melanoma grew more aggressively in BLNK−/− mice as compared to wild-type mice. As expected, tumor-infiltrating B cell numbers were decreased in BLNK−/− mice. Adoptive transfer of wild-type B cells into BLNK−/− mice attenuated B16F10 melanoma growth with increase numbers of B cells and T cells infiltrating into tumors. In addition, percentages of interferon-g and tumor necrosis factor-a producing tumor-infiltrating T cells were restored. These results support the concept that B cells enhance tumor immunity by promoting T cell infiltration into tumors and cytokine production within the tumor microenvironment. On the other hand, an ability of B cells to negatively regulate contact hypersensitivity and experimental autoimmune encephalomyelitis responses is attributed to B-cell provision of IL-10. The B-cell subset that produces IL-10 is identified as regulatory B cells (Bregs). In phosphatase and tensin homolog (PTEN)-deficient mice, Breg subset was significantly expanded and B16F10 melanoma grew more aggressively as compared to control mice. Tumor-infiltrating B cell numbers were significantly increased in B cell-specific PTEN-deficient mice. Interestingly, the tumor-infiltrating B cell consisted mostly of Bregs. These results suggest that Bregs negatively regulate tumor immunity within the tumor microenvironment. Taken together, B cells have dual aspects in tumor immunity.
http://dx.doi.org/10.1016/j.jdermsci.2017.02.184 http://dx.doi.org/10.1016/j.jdermsci.2017.02.185