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The effect of high-fat diet on the barrier functions and inflammatory status of human vascular endothelial cells
Abstracts / Atherosclerosis 252 (2016) e1ee196
Conclusions: Adiponectin was correlated with CS and CSR of carotid artery independently. This association was probably through the effects on matrix remodeling of carotid artery.
EAS16-1026, VASCULAR BIOLOGY: ENDOTHELIUM AND SMOOTH MUSCLE CELLS. THE LABILE IRON POOL AND PARAMETERS OF ARTERIAL WALL ELASTICITY
e167
increased percentage of apoptotic cells (37,1±6,9% versus 25,4±6,2%, p<0.05). Serum following the consumption of fatty meal affected neither occludin, nor VE-cadherin as compared to fasting serum (p>0.05). However, it caused 4-fold increase of IL-33 mRNA expression (p<0.01). Conclusions: High-fat diet may destabilize the endothelial barrier and initiate inflammatory processes, thus supporting atherogenesis
teník 2, R. Buchal 2, J. Poto ckov a 1, P. Kraml 1. 1 Faculty P. Risko 1, J. Pla Hospital Kralovske Vinohrady and Charles University - 3rd Medical Facult, 2nd Department of Internal Medicine, Prague 10, Czech Republic; 2 First Faculty of Medicine- Charles University Prague, Institute of Medical Biochemistry and Laboratory Diagnostics, Prague, Czech Republic Objectives: In the iron metabolism and the development of atherosclerosis macrophages play a major role. The aim of the study was to determine the redox-active iron (LIP, labile iron pool) in monocytes in cohort of patients in secondary prevention and healthy volunteers, and its relationship to arterial wall elasticity parameters. Ă
Methods: We examined 55 men (24 probands in secondary prevention, and 31 healthy volunteers). All respondents underwent basic physical examination, venous blood was collected for basic biochemical parameters and determination of the LIP in monocytes. Duplex sonography of the carotid arteries included assessment of intima-media thickness, elasticity index (YEM, PI, stiffness index B). We measured ankle-brachial index (ABI) and abdominal visceral fat thickness (VFT). Results: The patients in secondary prevention had significantly higher LIP values than did the control group (1.94 ± 0.46 mM vs. 1.62 ± 0.49 mM, p¼0.02). As we expected, the patients had significantly worse parameters of the arterial wall elasticity (YEM p <0.01; PI p <0.05), VFT (p <0.001) and ABI (p <0.001). In regression analysis LIP correlated only with VFT and ABI (p <0.05). Conclusions: The patients in secondary prevention have significantly higher levels of redox-active iron in monocytes. LIP positively correlates with ABI and VFT. NT 13671-4/2012. The study was supported by the IGA MZ CR
EAS16-0529, VASCULAR BIOLOGY: ENDOTHELIUM AND SMOOTH MUSCLE CELLS. THE EFFECT OF HIGH-FAT DIET ON THE BARRIER FUNCTIONS AND INFLAMMATORY STATUS OF HUMAN VASCULAR ENDOTHELIAL CELLS P. Gorzelak-Pabis, E. Luczak, K. Wojdan, M. Broncel, M. Chalubinski. Szpital.im Wl. Bieganskeigo, 1 Dept. of Internal Diseases and Clinical Pharmacology- Laboratory of Tissue ImmunopharmacologyMedical University of Lodz- Poland, Lodz, Poland Objectives: The vascular endothelium forms a barrier that controls flow of solutes and proteins and the entry of leukocytes into tissue. The impairment of endothelial barrier by lipids shown in in vitro studies may be one of the key factors of atherogenesis. The aim of our study was to assess the effects of serum following fatty meal consumption on barrier functions and inflammatory status on human vascular endothelial cells Methods: Human umbilical vein endothelial cells (HUVECs) were induced with serum of five healthy volunteers taken before and 3 hours following the consumption of standardized daily required dose of fatty meal. In sera, cholesterol and triglyceride levels were assessed. HUVEC integrity was measured in the of the Real-time Cell Electric Impedance Sensing system (RTCA-DP). mRNA expression of IL-33, tight (occludin) and adherens (VEcadherin) junction proteins was analyzed by real-time PCR. Additionally, viability and apoptosis with use of annexin-V and propidium iodide staining were assessed in flow cytometry. Results: In all patients, concentration of triglycerides at 3rd hour after a consumption of fatty meal was increased (110±37mg/dl vs 182±64mg/dl). Postprandial serum caused 20% decrease of HEVEC integrity as compared to fasting serum (p<0.0001). HUVEC disintegration was accompanied by
EAS16-0467, VASCULAR BIOLOGY: ENDOTHELIUM AND SMOOTH MUSCLE CELLS. MODIFYING THE GUT MICROBIOME WITH POLYPHENOLS AND A LECTIN LIMITED DIET IMPROVES ENDOTHELIAL FUNCTION S. Gundry. The International Heart and Lung Institute, Palm Springs, USA Objectives: Microbiome (MB) alterations promote inflammation and Endothelial Dysfunction (ED). A change in diet to alter MB, was studied for effects on ED, measured by Peripheral Arterial Tonometry (PAT) in 200 pts with vascular risk factors. Methods: Pts ate a diet of leafy greens, brassica vegetables, olive oil, nuts, radical reduction of grain, legumes, and fruits; and 4 oz amts of animal proteins and Casein A2 cheeses (The Matrix Protocol). Pts took 4,000 mg Fish Oil, 200mg Grape Seed Extract, 50 mg Pycnogenol, Grapefruit Seed Extract, 1 Tbs Balsamic Vinegar before meals, Glucomannan, and Inulin as prebiotics daily. Inflammatory markers were measured for 1 year. Endothelial Reactivity (ER) before and after a 5-minute arm occlusion using the EndoPAT 2000 (Itamar, Israel) was taken at baseline and 6 months. Results: TNF-alpha levels decreased from 4.5+/-1 to 2.5+/-0.4 (nl<2.5 pg/ ml;) hs-CRP decreased from 4.5+/-2 to 0.9+/-0.8mg/L. Baseline ER was 1.88+/-0.7 (range 1.0-3.3), with 145/200 pts (72%)having ED (less than 1.60). At 6 months, ER increased to 2.25+/-0.5 (range 1.2-3.6) (p<0.01). Conclusions: We conclude that a limited grain, legume, fruit diet with emphasis on greens, olive oil, nuts, limited animal proteins; with fish oil, polyphenols and prebiotic fibers to manipulate the MB, normalizes ER and inflammation.
EAS16-0880, VASCULAR BIOLOGY: ENDOTHELIUM AND SMOOTH MUSCLE CELLS. GENOMIC REARRANGEMENTS IN HUMAN ATHEROSCLEROTIC VASCULAR TISSUES A. Sleptcov 1, 2, M. Nazarenko 1, 2, I. Lebedev 1, 2, N. Skryabin 1, 2, O. Barbarash 3, V. Puzyrev 1, 2. 1 Research Institute of Medical Genetics, Laboratory of Population Genetics, Tomsk, Russia; 2 Tomsk State University, Laboratory of Human Ontogenetics, Tomsk, Russia; 3 Research Institute for Complex Issues of Cardiovascular Diseases, Department of Multifocal Atherosclerosis, Kemerovo, Russia Objectives: Our purpose was to investigate whether coronary arteryspecific DNA alterations are present in patients with cardiometabolic comorbidity. Methods: We have collected right coronary arteries in advanced atherosclerotic plaques, intact internal mammary arteries, and white blood cells derived from the same patient (n¼10). The artery samples from ten
Conclusions: Adiponectin was correlated with CS and CSR of carotid artery independently. This association was probably through the effects on matrix remodeling of carotid artery.
EAS16-1026, VASCULAR BIOLOGY: ENDOTHELIUM AND SMOOTH MUSCLE CELLS. THE LABILE IRON POOL AND PARAMETERS OF ARTERIAL WALL ELASTICITY
e167
increased percentage of apoptotic cells (37,1±6,9% versus 25,4±6,2%, p<0.05). Serum following the consumption of fatty meal affected neither occludin, nor VE-cadherin as compared to fasting serum (p>0.05). However, it caused 4-fold increase of IL-33 mRNA expression (p<0.01). Conclusions: High-fat diet may destabilize the endothelial barrier and initiate inflammatory processes, thus supporting atherogenesis
teník 2, R. Buchal 2, J. Poto ckov a 1, P. Kraml 1. 1 Faculty P. Risko 1, J. Pla Hospital Kralovske Vinohrady and Charles University - 3rd Medical Facult, 2nd Department of Internal Medicine, Prague 10, Czech Republic; 2 First Faculty of Medicine- Charles University Prague, Institute of Medical Biochemistry and Laboratory Diagnostics, Prague, Czech Republic Objectives: In the iron metabolism and the development of atherosclerosis macrophages play a major role. The aim of the study was to determine the redox-active iron (LIP, labile iron pool) in monocytes in cohort of patients in secondary prevention and healthy volunteers, and its relationship to arterial wall elasticity parameters. Ă
Methods: We examined 55 men (24 probands in secondary prevention, and 31 healthy volunteers). All respondents underwent basic physical examination, venous blood was collected for basic biochemical parameters and determination of the LIP in monocytes. Duplex sonography of the carotid arteries included assessment of intima-media thickness, elasticity index (YEM, PI, stiffness index B). We measured ankle-brachial index (ABI) and abdominal visceral fat thickness (VFT). Results: The patients in secondary prevention had significantly higher LIP values than did the control group (1.94 ± 0.46 mM vs. 1.62 ± 0.49 mM, p¼0.02). As we expected, the patients had significantly worse parameters of the arterial wall elasticity (YEM p <0.01; PI p <0.05), VFT (p <0.001) and ABI (p <0.001). In regression analysis LIP correlated only with VFT and ABI (p <0.05). Conclusions: The patients in secondary prevention have significantly higher levels of redox-active iron in monocytes. LIP positively correlates with ABI and VFT. NT 13671-4/2012. The study was supported by the IGA MZ CR
EAS16-0529, VASCULAR BIOLOGY: ENDOTHELIUM AND SMOOTH MUSCLE CELLS. THE EFFECT OF HIGH-FAT DIET ON THE BARRIER FUNCTIONS AND INFLAMMATORY STATUS OF HUMAN VASCULAR ENDOTHELIAL CELLS P. Gorzelak-Pabis, E. Luczak, K. Wojdan, M. Broncel, M. Chalubinski. Szpital.im Wl. Bieganskeigo, 1 Dept. of Internal Diseases and Clinical Pharmacology- Laboratory of Tissue ImmunopharmacologyMedical University of Lodz- Poland, Lodz, Poland Objectives: The vascular endothelium forms a barrier that controls flow of solutes and proteins and the entry of leukocytes into tissue. The impairment of endothelial barrier by lipids shown in in vitro studies may be one of the key factors of atherogenesis. The aim of our study was to assess the effects of serum following fatty meal consumption on barrier functions and inflammatory status on human vascular endothelial cells Methods: Human umbilical vein endothelial cells (HUVECs) were induced with serum of five healthy volunteers taken before and 3 hours following the consumption of standardized daily required dose of fatty meal. In sera, cholesterol and triglyceride levels were assessed. HUVEC integrity was measured in the of the Real-time Cell Electric Impedance Sensing system (RTCA-DP). mRNA expression of IL-33, tight (occludin) and adherens (VEcadherin) junction proteins was analyzed by real-time PCR. Additionally, viability and apoptosis with use of annexin-V and propidium iodide staining were assessed in flow cytometry. Results: In all patients, concentration of triglycerides at 3rd hour after a consumption of fatty meal was increased (110±37mg/dl vs 182±64mg/dl). Postprandial serum caused 20% decrease of HEVEC integrity as compared to fasting serum (p<0.0001). HUVEC disintegration was accompanied by
EAS16-0467, VASCULAR BIOLOGY: ENDOTHELIUM AND SMOOTH MUSCLE CELLS. MODIFYING THE GUT MICROBIOME WITH POLYPHENOLS AND A LECTIN LIMITED DIET IMPROVES ENDOTHELIAL FUNCTION S. Gundry. The International Heart and Lung Institute, Palm Springs, USA Objectives: Microbiome (MB) alterations promote inflammation and Endothelial Dysfunction (ED). A change in diet to alter MB, was studied for effects on ED, measured by Peripheral Arterial Tonometry (PAT) in 200 pts with vascular risk factors. Methods: Pts ate a diet of leafy greens, brassica vegetables, olive oil, nuts, radical reduction of grain, legumes, and fruits; and 4 oz amts of animal proteins and Casein A2 cheeses (The Matrix Protocol). Pts took 4,000 mg Fish Oil, 200mg Grape Seed Extract, 50 mg Pycnogenol, Grapefruit Seed Extract, 1 Tbs Balsamic Vinegar before meals, Glucomannan, and Inulin as prebiotics daily. Inflammatory markers were measured for 1 year. Endothelial Reactivity (ER) before and after a 5-minute arm occlusion using the EndoPAT 2000 (Itamar, Israel) was taken at baseline and 6 months. Results: TNF-alpha levels decreased from 4.5+/-1 to 2.5+/-0.4 (nl<2.5 pg/ ml;) hs-CRP decreased from 4.5+/-2 to 0.9+/-0.8mg/L. Baseline ER was 1.88+/-0.7 (range 1.0-3.3), with 145/200 pts (72%)having ED (less than 1.60). At 6 months, ER increased to 2.25+/-0.5 (range 1.2-3.6) (p<0.01). Conclusions: We conclude that a limited grain, legume, fruit diet with emphasis on greens, olive oil, nuts, limited animal proteins; with fish oil, polyphenols and prebiotic fibers to manipulate the MB, normalizes ER and inflammation.
EAS16-0880, VASCULAR BIOLOGY: ENDOTHELIUM AND SMOOTH MUSCLE CELLS. GENOMIC REARRANGEMENTS IN HUMAN ATHEROSCLEROTIC VASCULAR TISSUES A. Sleptcov 1, 2, M. Nazarenko 1, 2, I. Lebedev 1, 2, N. Skryabin 1, 2, O. Barbarash 3, V. Puzyrev 1, 2. 1 Research Institute of Medical Genetics, Laboratory of Population Genetics, Tomsk, Russia; 2 Tomsk State University, Laboratory of Human Ontogenetics, Tomsk, Russia; 3 Research Institute for Complex Issues of Cardiovascular Diseases, Department of Multifocal Atherosclerosis, Kemerovo, Russia Objectives: Our purpose was to investigate whether coronary arteryspecific DNA alterations are present in patients with cardiometabolic comorbidity. Methods: We have collected right coronary arteries in advanced atherosclerotic plaques, intact internal mammary arteries, and white blood cells derived from the same patient (n¼10). The artery samples from ten