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The effect of Naja nigricollis venom on blood clotting
Abstracts for Card Indexes
191
Further studies on the biochemistry of the toxins from the sea wasp Chironex fleckeri: H. D. CxoxE and T. E. B. KrErr, Toxicon,1971, 9,145. (Australian Defence Scientific Service, Defence Standards Laboratories, Melbourne, Victoria 3032, Australia) . Abstract-Extracts from the tentacles of Chironez Jleckeri (Cnidaria) were resolved into two lethal fractions by chromatography on CM-Sephadex C-S0 . These were apparently identical to the two fractions previously resolved on Sephadex G-200. The hemolysin in the extracts was also examined by thin-layer chromatography on Sephadex G-200 and by various electrophonetic techniques . It was shown that the hemolysin associated markedly with the various support media, which made electrophoresis of little use. The tentacle extracts and a toxin obtained by a milking technique were compared by chromatography and shown to be very similar if not identical in their biological activities . Some data on the stability of the hemolysin was also obtained .
Studies on puff adder (Bibs arietans) venom-I. Purification and properties of protease A : S. J. VAx Dhlt WALT and F. J. JouahItT, Toxicon, 1971, 9, 153 . (The National Chemical Research Laboratory, Council for Scientific and Industrial Research, P.O. Box 395, Pretoria, Republic of South Africa) . Abstract-The proteases from puff adder venom were separated by gradient elution from CM~ellulose, and protease A was further purified by Sephadex G-75 gel filtration. It was found to have a molecular weight of 21,400 and it showed a tendency to associate particularly at higher concentrations . The amino-acid composition and physical properties of protease A are reported . It had no esterase activity towards ATEE or TAME and could not be inhibited by DFP, TPCK, TLCIC or PMA. The enzyme requires the presence of calcium to prevent sutolysis.
In vitro anaphylactic response to staphylococcal enterotoxin : MAx E. McIrrrosx and D$Ax D. WATT, Toxicon,1971, 9,163. (Laboratory ofNeurological Research, LosAngeles County -University of Southern California Medical School, Los Angeles, California, U.S.A. and Division of Biological Sciences, Midwest Research Institute, Kansas City, Mo., U.S.A.). Abstract-Purified staphylococcal enterotoxin type B from strain S6 of Staphylococcus aureus is a specific antigen for in vitro anaphylaxis . The anaphylactic reaction is obtained in sensitized uterine horn and lung from theguinea pig and sensitized platelets, represented by whole-blood challenge, from the rabbit . Our results suggest that staphylococcal enterotoxin type B can be detected in the submicrogram range by in vitro anaphylaxis. Other methods of detection do not offer this level of sensitivity.
Effects of Naja nigricollis venom on blood and tissue histamine : A. H. MOIIAn~n, M. S. EIrSIgtOUGI and R. M. HAI~n, Toxicon, 1971, 9,169. (Department of Physiology, Faculty of Medicine Ein-Shams University, Cairo, Egypt) . Abstract-Ngjanigricollisvenom produces a marked increase of blood histamine and release of tissue histamine. This latter effect is inhibited by EDTA and Egyptian polyvalent antivenin (containing no specific antivenin against Ngja nigricollis venom) .
The effect of Naja nigricollis venom on blood clotting : A. H. MOHAMBD, M. S. EL-$EROUGI and R. M. HAS, Toxicon,1971, 9,173. (Department ofPhysiology, Faculty of Medicine, Ein-Shams University, Cairo, Egypt). Ab~strad-Ngja nigricollis venom has an in viva anticoagulant action which is heat-labile and is prevented by protamine and polyvalent horse anti-snake venom serum containing no specific antivenin against this venom. This anticoagulant action may be attributed partly to an antithromboplastin-like action and partly to heparin release. This venom has also a weak in vitro coagulant action which is masked in viva by the stronger anticoagulant effect .
191
Further studies on the biochemistry of the toxins from the sea wasp Chironex fleckeri: H. D. CxoxE and T. E. B. KrErr, Toxicon,1971, 9,145. (Australian Defence Scientific Service, Defence Standards Laboratories, Melbourne, Victoria 3032, Australia) . Abstract-Extracts from the tentacles of Chironez Jleckeri (Cnidaria) were resolved into two lethal fractions by chromatography on CM-Sephadex C-S0 . These were apparently identical to the two fractions previously resolved on Sephadex G-200. The hemolysin in the extracts was also examined by thin-layer chromatography on Sephadex G-200 and by various electrophonetic techniques . It was shown that the hemolysin associated markedly with the various support media, which made electrophoresis of little use. The tentacle extracts and a toxin obtained by a milking technique were compared by chromatography and shown to be very similar if not identical in their biological activities . Some data on the stability of the hemolysin was also obtained .
Studies on puff adder (Bibs arietans) venom-I. Purification and properties of protease A : S. J. VAx Dhlt WALT and F. J. JouahItT, Toxicon, 1971, 9, 153 . (The National Chemical Research Laboratory, Council for Scientific and Industrial Research, P.O. Box 395, Pretoria, Republic of South Africa) . Abstract-The proteases from puff adder venom were separated by gradient elution from CM~ellulose, and protease A was further purified by Sephadex G-75 gel filtration. It was found to have a molecular weight of 21,400 and it showed a tendency to associate particularly at higher concentrations . The amino-acid composition and physical properties of protease A are reported . It had no esterase activity towards ATEE or TAME and could not be inhibited by DFP, TPCK, TLCIC or PMA. The enzyme requires the presence of calcium to prevent sutolysis.
In vitro anaphylactic response to staphylococcal enterotoxin : MAx E. McIrrrosx and D$Ax D. WATT, Toxicon,1971, 9,163. (Laboratory ofNeurological Research, LosAngeles County -University of Southern California Medical School, Los Angeles, California, U.S.A. and Division of Biological Sciences, Midwest Research Institute, Kansas City, Mo., U.S.A.). Abstract-Purified staphylococcal enterotoxin type B from strain S6 of Staphylococcus aureus is a specific antigen for in vitro anaphylaxis . The anaphylactic reaction is obtained in sensitized uterine horn and lung from theguinea pig and sensitized platelets, represented by whole-blood challenge, from the rabbit . Our results suggest that staphylococcal enterotoxin type B can be detected in the submicrogram range by in vitro anaphylaxis. Other methods of detection do not offer this level of sensitivity.
Effects of Naja nigricollis venom on blood and tissue histamine : A. H. MOIIAn~n, M. S. EIrSIgtOUGI and R. M. HAI~n, Toxicon, 1971, 9,169. (Department of Physiology, Faculty of Medicine Ein-Shams University, Cairo, Egypt) . Abstract-Ngjanigricollisvenom produces a marked increase of blood histamine and release of tissue histamine. This latter effect is inhibited by EDTA and Egyptian polyvalent antivenin (containing no specific antivenin against Ngja nigricollis venom) .
The effect of Naja nigricollis venom on blood clotting : A. H. MOHAMBD, M. S. EL-$EROUGI and R. M. HAS, Toxicon,1971, 9,173. (Department ofPhysiology, Faculty of Medicine, Ein-Shams University, Cairo, Egypt). Ab~strad-Ngja nigricollis venom has an in viva anticoagulant action which is heat-labile and is prevented by protamine and polyvalent horse anti-snake venom serum containing no specific antivenin against this venom. This anticoagulant action may be attributed partly to an antithromboplastin-like action and partly to heparin release. This venom has also a weak in vitro coagulant action which is masked in viva by the stronger anticoagulant effect .