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The effect of short-term lipid infusion on liver function and biliary secretion in rats
Section 12 - LIVER
Date: Tuesday, September 8 13.30-l Chair: M. Deutz (Netherlands)
well as the lithogenic index (CSI). The bile flow of all groups was similar. In the liver no significant change in weight and function tests were observed. Liver cholesterol content was 50% higher in the LCT group and showed greater fatty infiltration on histologic examination. Although large quantities of cholesterol were detected no radiolabeled cholesterol was found in the liver and bile. This study indicates that LCT emulsion causes an increase of cholesterol content of the liver and the bile that probably originates from enhanced mobilization of cholesterol to the liver. It seems likely that the lithogenicity of the bile in rats is directly related to the type of triglycerides infused.
4.30
Rubin, M. (Petah-Tiqva) The Effect of ShortTerm Lipid Infusion on Liver Function and Biliary Secretion in Rats Cabre, E. (Badalona) Effect of Portal-Systemic Shunting on the Plasma Lipid Polyunsaturated Fatty Acid (PUFA) Content in Liver Cirrhosis (LC) Radoch, E. (Hannover 61) Peripheral Insulin Resistance as Main Cause of Impaired Glucose Tolerance in Patients with Liver Cirrhosis Nielsen, K. (Copenhagen) Nitrogen Balance and Whole Body Protein Turnover during Hyperalimentation of Malnourished Patients with Liver Cirrhosis Nielsen, K. (Copenhagen) Protein Requirement and Retention in Malnourished Patients with Liver Cirrhosis
P.120 Effect of portal-systemic shunting on the plasma lipid polyunsaturated fatty acid (PUFA) content in liver cirrhosis (LC)
Skullman, S. (Linkiiping) Malnutrition Impair Protein Synthesis in Regenerating Liver
E. Cabr6, M. de Ramdn, R. Planas, J.C. Quer, J. Boix, C. Pastor, J.M. Llovet and M.A. Gassull Dept. of Gastroenterology, Hosp. Germans Trias i Pujol, Badalona. Spain
Garcia-Vielba, J. (Leon) Total Parenteral Nutrition-Associated Alterations of Bilirubin Metabolism in Rats
Plasma PUFA deficit occurs in advanced LC due to both hepatocellular failure and malnutrition. Indirect data suggest that portal-systemic shunting might also contribute to PUFA deficit in LC: 1) A substantial amount of FA are absorbed via the portal vein in LC, 2) PUFA levels are lower in LC with or without chronic encephalopathy, and 3) in rats, eicosanoid biosynthesis increases after portacaval anastomosis (PCA). Aim: To investigate the effect of PCA on the plasma FA profile in LC. Methods: 22 Child-Pugh class A and B cirrhotics (18M, 4F, 54.6 _+ 2.0 yrs) were randomized to be treated with either a PCA (n = 11) or vasoactive drugs (propranolol + isosorbide mononitrate) (control group; n = 11) 3 weeks after recovering from an episode of variceal bleeding. Routine liver function tests, nutritional parameters (TSF, MAMC, serum albumin) and plasma FA profile (capillary column GLC) were measured before and 3 and 6 months after PCA or starting drugs. MANOVA for repeated measured and unpaired t-test were used for statistical analysis. Results: No changes in percent plasma saturated, monoenoic, and essential FA were found in either group. Long-chain n6 PUFA (specially arachidonate) decreased during follow-up in PCA (MANOVA: p = 0.027) but not in control patients (p = 0.007 between groups at 6 mo.). Percent long-chain n3 PUFA (mainly docosahexanoate) increased over the first 3 mo. in control (MANOVA: p = 0.03) but not in PCA patients (p = 0.033 between groups). These changes occurred in spite that parameters of hepatic synthesis and nutritional status did not differ between both groups during follow-up. Conclusions: 1) Portacaval shunting is an additional factor for plasma PU FA deficit in liver cirrhosis. 2) This phenomenon appears not to be related to impairment in liver function or nutritional status.
Viell, B. (Mannheim 1) Non-Transmitter Indoles, Kynurenine and Encephalopathy in Liver Cirrhosis (LC) P.119 The effect of short-term lipid infusion on liver function and biliary secretion in rats M. Rubin. Z. Halpern. A. Moser, A. Devir, E. AntebiandA. Wennberg Sackler Faculty of Medicine, Tel-Aviv University, Israel and Vitrum. Sweden Lipid infusion is alleged to result in hepatobiliary complications, therefore the effect of different lipid emulsions was studied in 65 rats. Rats were infused for 48 hours with a 20% lipid emulsion (35 mg/kg/day) while being on fat free diet (Table). Bile duct was cannulated and bile was collected during the last hour of infusion for lipid composition and bile flow determination. Liver function tests, cholesterol content, weight, and histology were determined. In addition LCT Cr’41 radiolabelled was infused in two rats. Bile composition Cholesterol Phospholipid Bile salts CSI Bile composition Cholesterol Phospholipid Bile salts CSI
‘p i 0.05.tp <
Control Saline (11) 0.16f0.07 0.88f0.34 16.63k6.3 0.64-tO.38 MCT + LCT Lipofundin (10) 0.21 f0.04 1 .Ol kO.27 17.9ozt3.54 0.63f0.15 0.010
LCT Endolipid (9) 0.2s*0.13t 1.33kO.60’ 16.75i7.50 0.82kO.29
lntralipid (10) 0.28*0.12t 1.35kO.37 20.24k5.88 0.72&0.41
Structured (10) 0.19+0.05 0.98 f 0.24 16.96k4.15 0.63+0.15
MCT MCT (9) 0.22kO.04 1.12+0.20 21.42t6.13 0.57kO.08
Biliary lipid composition of the LCT groups only showed a significant increase in cholesterol and phospholipid levels as 90
Date: Tuesday, September 8 13.30-l Chair: M. Deutz (Netherlands)
well as the lithogenic index (CSI). The bile flow of all groups was similar. In the liver no significant change in weight and function tests were observed. Liver cholesterol content was 50% higher in the LCT group and showed greater fatty infiltration on histologic examination. Although large quantities of cholesterol were detected no radiolabeled cholesterol was found in the liver and bile. This study indicates that LCT emulsion causes an increase of cholesterol content of the liver and the bile that probably originates from enhanced mobilization of cholesterol to the liver. It seems likely that the lithogenicity of the bile in rats is directly related to the type of triglycerides infused.
4.30
Rubin, M. (Petah-Tiqva) The Effect of ShortTerm Lipid Infusion on Liver Function and Biliary Secretion in Rats Cabre, E. (Badalona) Effect of Portal-Systemic Shunting on the Plasma Lipid Polyunsaturated Fatty Acid (PUFA) Content in Liver Cirrhosis (LC) Radoch, E. (Hannover 61) Peripheral Insulin Resistance as Main Cause of Impaired Glucose Tolerance in Patients with Liver Cirrhosis Nielsen, K. (Copenhagen) Nitrogen Balance and Whole Body Protein Turnover during Hyperalimentation of Malnourished Patients with Liver Cirrhosis Nielsen, K. (Copenhagen) Protein Requirement and Retention in Malnourished Patients with Liver Cirrhosis
P.120 Effect of portal-systemic shunting on the plasma lipid polyunsaturated fatty acid (PUFA) content in liver cirrhosis (LC)
Skullman, S. (Linkiiping) Malnutrition Impair Protein Synthesis in Regenerating Liver
E. Cabr6, M. de Ramdn, R. Planas, J.C. Quer, J. Boix, C. Pastor, J.M. Llovet and M.A. Gassull Dept. of Gastroenterology, Hosp. Germans Trias i Pujol, Badalona. Spain
Garcia-Vielba, J. (Leon) Total Parenteral Nutrition-Associated Alterations of Bilirubin Metabolism in Rats
Plasma PUFA deficit occurs in advanced LC due to both hepatocellular failure and malnutrition. Indirect data suggest that portal-systemic shunting might also contribute to PUFA deficit in LC: 1) A substantial amount of FA are absorbed via the portal vein in LC, 2) PUFA levels are lower in LC with or without chronic encephalopathy, and 3) in rats, eicosanoid biosynthesis increases after portacaval anastomosis (PCA). Aim: To investigate the effect of PCA on the plasma FA profile in LC. Methods: 22 Child-Pugh class A and B cirrhotics (18M, 4F, 54.6 _+ 2.0 yrs) were randomized to be treated with either a PCA (n = 11) or vasoactive drugs (propranolol + isosorbide mononitrate) (control group; n = 11) 3 weeks after recovering from an episode of variceal bleeding. Routine liver function tests, nutritional parameters (TSF, MAMC, serum albumin) and plasma FA profile (capillary column GLC) were measured before and 3 and 6 months after PCA or starting drugs. MANOVA for repeated measured and unpaired t-test were used for statistical analysis. Results: No changes in percent plasma saturated, monoenoic, and essential FA were found in either group. Long-chain n6 PUFA (specially arachidonate) decreased during follow-up in PCA (MANOVA: p = 0.027) but not in control patients (p = 0.007 between groups at 6 mo.). Percent long-chain n3 PUFA (mainly docosahexanoate) increased over the first 3 mo. in control (MANOVA: p = 0.03) but not in PCA patients (p = 0.033 between groups). These changes occurred in spite that parameters of hepatic synthesis and nutritional status did not differ between both groups during follow-up. Conclusions: 1) Portacaval shunting is an additional factor for plasma PU FA deficit in liver cirrhosis. 2) This phenomenon appears not to be related to impairment in liver function or nutritional status.
Viell, B. (Mannheim 1) Non-Transmitter Indoles, Kynurenine and Encephalopathy in Liver Cirrhosis (LC) P.119 The effect of short-term lipid infusion on liver function and biliary secretion in rats M. Rubin. Z. Halpern. A. Moser, A. Devir, E. AntebiandA. Wennberg Sackler Faculty of Medicine, Tel-Aviv University, Israel and Vitrum. Sweden Lipid infusion is alleged to result in hepatobiliary complications, therefore the effect of different lipid emulsions was studied in 65 rats. Rats were infused for 48 hours with a 20% lipid emulsion (35 mg/kg/day) while being on fat free diet (Table). Bile duct was cannulated and bile was collected during the last hour of infusion for lipid composition and bile flow determination. Liver function tests, cholesterol content, weight, and histology were determined. In addition LCT Cr’41 radiolabelled was infused in two rats. Bile composition Cholesterol Phospholipid Bile salts CSI Bile composition Cholesterol Phospholipid Bile salts CSI
‘p i 0.05.tp <
Control Saline (11) 0.16f0.07 0.88f0.34 16.63k6.3 0.64-tO.38 MCT + LCT Lipofundin (10) 0.21 f0.04 1 .Ol kO.27 17.9ozt3.54 0.63f0.15 0.010
LCT Endolipid (9) 0.2s*0.13t 1.33kO.60’ 16.75i7.50 0.82kO.29
lntralipid (10) 0.28*0.12t 1.35kO.37 20.24k5.88 0.72&0.41
Structured (10) 0.19+0.05 0.98 f 0.24 16.96k4.15 0.63+0.15
MCT MCT (9) 0.22kO.04 1.12+0.20 21.42t6.13 0.57kO.08
Biliary lipid composition of the LCT groups only showed a significant increase in cholesterol and phospholipid levels as 90