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The Effect of siRNA-Ku70 on the Expression of Apoptosis-Related Genes in A549DDP
October 2010, Vol 138, No. 4_MeetingAbstracts Poster Presentations: Tuesday, November 2, 2010 | October 2010
The Effect of siRNA-Ku70 on the Expression of Apoptosis-Related Genes in A549DDP Jie Zhang, PhD; Ke Wang, PhD 2nd hospital of Jilin University, Changchun, Peoples Rep of China Chest. 2010;138(4_MeetingAbstracts):264A. doi:10.1378/chest.10807 Abstract PURPOSE: To investigate the effect of SiRNA-Ku70 in drug-resistance in A549DDP cell line. METHODS: Expression of apoptosis-related genes was examined by PCR-Array assay. RESULTS: Expression of BAX,P53 and P73 increased significantly whereas the expression of TRAF and BFAR decreased in SiRNA-Ku70 A549DDP cells compared to A549DDP cells (P< 0.05) . CONCLUSION: These results show that Ku70 might play an important role in drugresistance in A549DDP cell line. Silencing of Ku70 can be attributed to the elevation of apoptotic genes- BAX, P53 and P73 expression; reduction of anti-apoptotic genes TRAF and BFAR. This study suggests that silencing of Ku70 might reverse the drugresistance in A549DDP cell line. CLINICAL IMPLICATIONS: Ku70 might be a new target for the drug-resistant reversion. DISCLOSURE: Jie Zhang, No Financial Disclosure Information; No Product/Research Disclosure Information 12:45 PM - 2:00 PM
The Effect of siRNA-Ku70 on the Expression of Apoptosis-Related Genes in A549DDP Jie Zhang, PhD; Ke Wang, PhD 2nd hospital of Jilin University, Changchun, Peoples Rep of China Chest. 2010;138(4_MeetingAbstracts):264A. doi:10.1378/chest.10807 Abstract PURPOSE: To investigate the effect of SiRNA-Ku70 in drug-resistance in A549DDP cell line. METHODS: Expression of apoptosis-related genes was examined by PCR-Array assay. RESULTS: Expression of BAX,P53 and P73 increased significantly whereas the expression of TRAF and BFAR decreased in SiRNA-Ku70 A549DDP cells compared to A549DDP cells (P< 0.05) . CONCLUSION: These results show that Ku70 might play an important role in drugresistance in A549DDP cell line. Silencing of Ku70 can be attributed to the elevation of apoptotic genes- BAX, P53 and P73 expression; reduction of anti-apoptotic genes TRAF and BFAR. This study suggests that silencing of Ku70 might reverse the drugresistance in A549DDP cell line. CLINICAL IMPLICATIONS: Ku70 might be a new target for the drug-resistant reversion. DISCLOSURE: Jie Zhang, No Financial Disclosure Information; No Product/Research Disclosure Information 12:45 PM - 2:00 PM