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The effect of UVA irradiation and indomethacin treatment on the contact hypersensitivity reaction in the mice
CANDIDACIDAL PROTEINS PURIFIED PROM HUhfAN EPIDERMIS wERERBvJxALeD TO BE HISTIDINE-RICH PROTEIN @LAGGRIN) AND Hl HISTDNES. MASATO KASHWA, MASARU OKUDA: and HISASHI TAKAHASHI Department of Dermatology, Teikyo Umversity, School of Medicine, Tokyo. Citric acid extractable proteins of human epidermis showed candidacidal activity! which may play a role in the non-immuological defense mechamsm. T%eactive proteins were fractionated into at least two groups by Mono S column chromatography using an HPLC. The first group was arginine and histidine rich, and reacred to an anti fdaggrin antibody. The second group was further fractionated into three purified proteins by Phenyl5PW RP column chromatography. They reacxed to anti Hl histone antibody, and their amino acid cmnpositions were almost the same as those of subclasses of Hl histone. The histidine-rich protein and Hl histones might be good candidates for chemical barrier in epidermis because in skin candidiasis the micrwrganisms cannot invade deeper than granular layer where the histidine-rich protein is synthesized, and HI histone may be released from disappearing nuclei.
ANTIGEN PRESENTING CELLS AND T LYMPHOCYTE INDUCTION PHASE OF CONTACT HYPERSENSITIVITY
IN THE
HIDEKI MORITA, MIKA HORI, TAKAKO KIHARA, YUKIO KITANO, AND SEICHIRO SAGAMI. Depatment of Dermatology, Hyogo College of Medicine, Nishinomiya. When FITC was painted on the abdominal skin of mice, FITCcells appeared in 24 hours in the inguinal lymph node.They were relatively largein size,and was not T lymphocyte. When FITC was paintedon eithera murine tailskinor skinpre-treated with tape stripping, the number of FITCcellsintheinguinal lymph node was significantly less then those in the positive control. In the inguinal lymph nodes on the 4th day after PC1 had been paintedon theabdominalskinof mice,L3T4+ cells, which expressed IL-2R,increased in number. On the other hand, when PC1 was painted on either tail skin or tape-stripped skin L3T4* IL-2R+cells did not increase in the regional lymph node.
THE EFFECT OF UVA IRRADIATION AND INDOMETHACIN TREATMENT ON THE CONTACT HYPERSENSITIVITY REACTION IN THE MICE YCON-KEE PARK, SEUNG KYUNG HANN. MOO YON CHO Department of Dermatology, Yonsei University College of Medicine, Seoul, Korea To study the effect of large dose(800Jlcm2) of UVA irradiation and indomethacin treatment on contact hypersensitivity reaction in the mice, the following ftems were evaluated: the change of contact hypersensitivity(CH) following UVA irradiation; the change in prostaglandin E following UVA irradiation; the change in CH following indomethacin treatment ; the change in CH following combined indomethacin and UVA treatment. The results are summarized as follows. 1. Systemic suppression of CH was observed in the DNFB painting on the WA non-irradiated abdomen following UVA irradiation on the backs of mice. 2. The plasma level of pmstaglandin E in UVA irradiated mice increased the first day after irradiation and then gradually decreased up to 10 days following irradiation. 3. The CH was depressed in the indomethacin only treated mice. The CH was depressed in the combined indomethacin and UVA treated mice as much as indomethacin only treated mice.
DTH TO NICKEL SULFATE IS ASSOCIATED WITH I-A REGION IN MICE.
NORIHISA ISHII, RARUMI ISHII, HIROSHI NARAJIMA. of Dermatology, Yokohama City Univ. School
Department
Medicine,
of
Yokohama.
Genetic control of delayed type hypersensitivity (DTH) to nickel sulfate antigen was studied using various strains of inbred mice. Mice with class II Ad,k,s hsplotypes showed a high magnitude of response, whereas those with class I[ Ab,f were low responders. These results indicate that nickel sulfate DTH may be influenced by the I-A region of H-Z. Transfer experiments revealed that these responses might be mediated by L3T4+, lyt-2- T cells.
A STUDY OF IRRITANT FOTENCY USING PATCH TEST, SKIN ORGAN CULTUREANDtXXfMALHU1UMANKERATINOCrPECULTUREME'IVODS HEE CHUL EUN, JOON MO YANG, YCXJSHIN LEE Department of Dermatology, College of Medicine, Seoul National University Hospital, 28 Chongno-ku Yunkun-dong, SEOUL, KOREA For the detection of skin irritant potency, animal models and human models have been used. Recently, skin organ culture and cell cutnre ntxlelsare being tried. To determine the difference between in viva and in vitro models for the detection of irritancy, the authors patch tested with 3 different irritants to 11 human volunteers and checked the clinical score and measured the reactions with laser Doppler flowneter. The morphologic observation of skin organ cultured spacimens and MPT on the normal human keratinczyte cultured cells were pxformed after incubation with 3 irritants of different concentrations. There was some correlation between each model, however some difference was also observed in the detection of irritant ptencey. Limitation of each model is also discussed.
PRO~ICN PERIPHERAL
OF IL-4, IL-2, IFWI AND 'RF-qBY BL(xID LYMPEX!fl'ES IN AlllPIC DEIlMATITIS
L-mswAKA FURUE, TAKENORI -HI, EWYUKI OcJm, HIDEMI N'+KAGV& YAS.M%A ISHIBASHI, YL?TAI0HS?!SAKI*,KAZUAKI HAMA* Univ. of Tokyo, on0 Pharmaceutical Co.*
The prcxluctionof hw IL-~, IL-2, IF?+Iand l'w-aby Ipripheral blmd lyqhoqte s (PBL) was examined in healthy control (n=l9), Or in patients with atopic dermatitis (AD, n= 17) or with other skin diseases (n=5). PBL (3xlO"/ml)~JX stimulated with Con A(lOfl/ml) and culture supernatantwas assayed 24hr after stwation. EJOsignificant difference of the production of IM and Ii%-r was observed in AD or in non AD control. In contrast, the production of IL-2 01 'INF-o(was significantly lowar in AD than that of healthy control. No correlation ms observed b&ween IgE and ths production of these fourcytokines in AD.
ANTIGEN PRESENTING CELLS AND T LYMPHOCYTE INDUCTION PHASE OF CONTACT HYPERSENSITIVITY
IN THE
HIDEKI MORITA, MIKA HORI, TAKAKO KIHARA, YUKIO KITANO, AND SEICHIRO SAGAMI. Depatment of Dermatology, Hyogo College of Medicine, Nishinomiya. When FITC was painted on the abdominal skin of mice, FITCcells appeared in 24 hours in the inguinal lymph node.They were relatively largein size,and was not T lymphocyte. When FITC was paintedon eithera murine tailskinor skinpre-treated with tape stripping, the number of FITCcellsintheinguinal lymph node was significantly less then those in the positive control. In the inguinal lymph nodes on the 4th day after PC1 had been paintedon theabdominalskinof mice,L3T4+ cells, which expressed IL-2R,increased in number. On the other hand, when PC1 was painted on either tail skin or tape-stripped skin L3T4* IL-2R+cells did not increase in the regional lymph node.
THE EFFECT OF UVA IRRADIATION AND INDOMETHACIN TREATMENT ON THE CONTACT HYPERSENSITIVITY REACTION IN THE MICE YCON-KEE PARK, SEUNG KYUNG HANN. MOO YON CHO Department of Dermatology, Yonsei University College of Medicine, Seoul, Korea To study the effect of large dose(800Jlcm2) of UVA irradiation and indomethacin treatment on contact hypersensitivity reaction in the mice, the following ftems were evaluated: the change of contact hypersensitivity(CH) following UVA irradiation; the change in prostaglandin E following UVA irradiation; the change in CH following indomethacin treatment ; the change in CH following combined indomethacin and UVA treatment. The results are summarized as follows. 1. Systemic suppression of CH was observed in the DNFB painting on the WA non-irradiated abdomen following UVA irradiation on the backs of mice. 2. The plasma level of pmstaglandin E in UVA irradiated mice increased the first day after irradiation and then gradually decreased up to 10 days following irradiation. 3. The CH was depressed in the indomethacin only treated mice. The CH was depressed in the combined indomethacin and UVA treated mice as much as indomethacin only treated mice.
DTH TO NICKEL SULFATE IS ASSOCIATED WITH I-A REGION IN MICE.
NORIHISA ISHII, RARUMI ISHII, HIROSHI NARAJIMA. of Dermatology, Yokohama City Univ. School
Department
Medicine,
of
Yokohama.
Genetic control of delayed type hypersensitivity (DTH) to nickel sulfate antigen was studied using various strains of inbred mice. Mice with class II Ad,k,s hsplotypes showed a high magnitude of response, whereas those with class I[ Ab,f were low responders. These results indicate that nickel sulfate DTH may be influenced by the I-A region of H-Z. Transfer experiments revealed that these responses might be mediated by L3T4+, lyt-2- T cells.
A STUDY OF IRRITANT FOTENCY USING PATCH TEST, SKIN ORGAN CULTUREANDtXXfMALHU1UMANKERATINOCrPECULTUREME'IVODS HEE CHUL EUN, JOON MO YANG, YCXJSHIN LEE Department of Dermatology, College of Medicine, Seoul National University Hospital, 28 Chongno-ku Yunkun-dong, SEOUL, KOREA For the detection of skin irritant potency, animal models and human models have been used. Recently, skin organ culture and cell cutnre ntxlelsare being tried. To determine the difference between in viva and in vitro models for the detection of irritancy, the authors patch tested with 3 different irritants to 11 human volunteers and checked the clinical score and measured the reactions with laser Doppler flowneter. The morphologic observation of skin organ cultured spacimens and MPT on the normal human keratinczyte cultured cells were pxformed after incubation with 3 irritants of different concentrations. There was some correlation between each model, however some difference was also observed in the detection of irritant ptencey. Limitation of each model is also discussed.
PRO~ICN PERIPHERAL
OF IL-4, IL-2, IFWI AND 'RF-qBY BL(xID LYMPEX!fl'ES IN AlllPIC DEIlMATITIS
L-mswAKA FURUE, TAKENORI -HI, EWYUKI OcJm, HIDEMI N'+KAGV& YAS.M%A ISHIBASHI, YL?TAI0HS?!SAKI*,KAZUAKI HAMA* Univ. of Tokyo, on0 Pharmaceutical Co.*
The prcxluctionof hw IL-~, IL-2, IF?+Iand l'w-aby Ipripheral blmd lyqhoqte s (PBL) was examined in healthy control (n=l9), Or in patients with atopic dermatitis (AD, n= 17) or with other skin diseases (n=5). PBL (3xlO"/ml)~JX stimulated with Con A(lOfl/ml) and culture supernatantwas assayed 24hr after stwation. EJOsignificant difference of the production of IM and Ii%-r was observed in AD or in non AD control. In contrast, the production of IL-2 01 'INF-o(was significantly lowar in AD than that of healthy control. No correlation ms observed b&ween IgE and ths production of these fourcytokines in AD.