- Email: [email protected]
The effect of watering frequency on the welfare quality of dairy cow
S92
Abstracts / Journal of Biotechnology 256S (2017) S44–S116
The purpose of this study was to determine the influence of hemodialysis in decreasing hemoglobin in acute renal patients. All patients received the same dose of sodium heparin (100 U.I./kg) before every hemodialysis session and the same hemodialysis blood lines, dialyzer size (15 sqm), bicarbonate cartridge 650 g NaHCO3 and dialysate solutions with K+ 2 mmol/l, Na+ 139 mmol/l, Ca++ 1.5 mmol/l, Mg++ 0.5 mmol/l, Cl− 106 mmol/l, HCO3 − 36 mmol/l, Acetate 3 mmol/l, Glc. 1 g/l, Osmolarity 296 mOsm/l have been used. The results showed that hemoglobin levels have decreased using hemodialysis with an average of 0.95% in each session and that we have to medicate our acute renal injury patients in order to maintain the hemoglobin levels in the normal range. http://dx.doi.org/10.1016/j.jbiotec.2017.06.1113 The effect of watering frequency on the welfare quality of dairy cow Silvana Popescu 1,∗ , Cristin Borda 1 , Eva Andrea Diugan 2 , Daniela Oros 1 , Mihaela Niculae 1 , Carmen Dana Sandru 1 1
Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania 2 The National Forest Administration Romsilva, Bucuresti, Romania E-mail address: [email protected] (S. Popescu). The aim of this research was to investigate the effect of the watering frequency on the welfare quality of dairy cows. The study was done in 54 dairy farms with free stall housing in Transylvania. The farms were included in three groups, according to their watering practices: A (20 farms): watering once a day, B (20 farms): watering twice a day and C (14 farms): free access to water. 4146 dairy cows ® were assessed by the Welfare Quality protocol for dairy cows and the data were statistically processed. The medians of the scores for welfare criteria and principles were significantly (P < 0.05) higher in the C farms, compared with the A and B farms. The A farms were in the “Acceptable” (12 farms) and “Not classified” (8 farms) categories, the B farms were categorised as “Enhanced” (4 farms), “Acceptable” (14 farms) and “Not classified” (2 farms), while the C farms were categorised as “Enhanced” (12 farms) and “Acceptable” (2 farms). The welfare quality of dairy cows was significantly better in the farms providing permanent access to water to the cows. The results indicate the need for permanent access to water for an improved welfare of dairy cows. http://dx.doi.org/10.1016/j.jbiotec.2017.06.1114 Quality of drinking water in rural areas of western Romania with reference to parasites Kalman Imre ∗ , Adriana Morar, Marius Ilie, Florin Hora, Corina Badea, Mirela Imre Department of Animal Production and Veterinary Public Health, Faculty of Veterinary Medicine, Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” from Timisoara, Romania E-mail address: kalman [email protected] (K. Imre). Safe drinking water sources is a general concern within the European Union countries. Nowadays, water wells constitute a very important untreated potable water sources in many rural areas
of western Romania. However, they may become contaminated with different waterborne pathogens posing a risk for the human health. In this study, a total of 22 private household and community drinking water wells were investigated for the presence of “Giardia” spp. and “Cptosporidium” spp. Water samples were collected by microfiber filtration, concentrated in agreement with USEPA 1623 protocol, and screened for the pathogens presence through immunofluorescence microscopy and PCR techniques. Two samples (9.1%) showed positive results with both techniques for “Giardia”, but no “Cryptosporidium” was identified. The ruminant origin “G. duodenalis” assemblage E and the human origin sub-assemblage AII were recorded. The results indicate that the drinking water wells are potentially under the influence of other neighbor possible contaminated surface waters and can jeopardize the public health. Acknowledgements: This work was supported by a grant of the Romanian National Authority for Scientific Research and Innovation, CNCS – UEFISCDI, project number PN-II-RU-TE-2014-4-1300. http://dx.doi.org/10.1016/j.jbiotec.2017.06.1115 Bioimaging of modified bacterial nanocellulose using optical and electron microscopy Andrey Nikolaevich Agafonov 1,∗ , Marina Yurievna Yazykova 2 , Arina Ilinichna Sizova 2 1
Department of Nanoengineering, Samara National Research University, Samara, Russia 2 Department of Biochemistry, Biotechnology and Bioengineering, Samara National Research University, Samara, Russia E-mail address: [email protected] (A.N. Agafonov). Bacterial nanocellulose (BNC) is a natural product with high potential for biomedical application. As a novel wound dressing material, it needs appropriate modification to obtain antimicrobial activity. In the present study 6-O-metilsulfonilaminoatsetat and 6-O-tolilsulfonilaminoatsetat of BCN (product of bacteria Gluconacetobacter sucrofermentans B-11267) were preparated by modification of a cellulose ester and structure analysis to reveal alteration of micropores’ number from control sample was maid. Bright-field optical microscopy (Biomed-4) and scanning electron microscopy (FEI Quanta 200) techniques were used for these purposes. Control sample showed typical for BNC threedimensional netted composition with random arrangement of ribbon-like microfibrils 0.1–1 m. On its surface, cylindrical micropores 0.1–2 m apparent size with depth of lying down within 5 m also occurred. In spite of essential alterations in modified samples’ structure of microfibrils, stratified structure of the surface and micropores’ system were kept. However, number of micropores per unit area significantly decreased and was reduced by an order of magnitude in both modified samples. To come to conclusion, it could be explained by incorporation of functional groups during modification process. http://dx.doi.org/10.1016/j.jbiotec.2017.06.1116
Abstracts / Journal of Biotechnology 256S (2017) S44–S116
The purpose of this study was to determine the influence of hemodialysis in decreasing hemoglobin in acute renal patients. All patients received the same dose of sodium heparin (100 U.I./kg) before every hemodialysis session and the same hemodialysis blood lines, dialyzer size (15 sqm), bicarbonate cartridge 650 g NaHCO3 and dialysate solutions with K+ 2 mmol/l, Na+ 139 mmol/l, Ca++ 1.5 mmol/l, Mg++ 0.5 mmol/l, Cl− 106 mmol/l, HCO3 − 36 mmol/l, Acetate 3 mmol/l, Glc. 1 g/l, Osmolarity 296 mOsm/l have been used. The results showed that hemoglobin levels have decreased using hemodialysis with an average of 0.95% in each session and that we have to medicate our acute renal injury patients in order to maintain the hemoglobin levels in the normal range. http://dx.doi.org/10.1016/j.jbiotec.2017.06.1113 The effect of watering frequency on the welfare quality of dairy cow Silvana Popescu 1,∗ , Cristin Borda 1 , Eva Andrea Diugan 2 , Daniela Oros 1 , Mihaela Niculae 1 , Carmen Dana Sandru 1 1
Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania 2 The National Forest Administration Romsilva, Bucuresti, Romania E-mail address: [email protected] (S. Popescu). The aim of this research was to investigate the effect of the watering frequency on the welfare quality of dairy cows. The study was done in 54 dairy farms with free stall housing in Transylvania. The farms were included in three groups, according to their watering practices: A (20 farms): watering once a day, B (20 farms): watering twice a day and C (14 farms): free access to water. 4146 dairy cows ® were assessed by the Welfare Quality protocol for dairy cows and the data were statistically processed. The medians of the scores for welfare criteria and principles were significantly (P < 0.05) higher in the C farms, compared with the A and B farms. The A farms were in the “Acceptable” (12 farms) and “Not classified” (8 farms) categories, the B farms were categorised as “Enhanced” (4 farms), “Acceptable” (14 farms) and “Not classified” (2 farms), while the C farms were categorised as “Enhanced” (12 farms) and “Acceptable” (2 farms). The welfare quality of dairy cows was significantly better in the farms providing permanent access to water to the cows. The results indicate the need for permanent access to water for an improved welfare of dairy cows. http://dx.doi.org/10.1016/j.jbiotec.2017.06.1114 Quality of drinking water in rural areas of western Romania with reference to parasites Kalman Imre ∗ , Adriana Morar, Marius Ilie, Florin Hora, Corina Badea, Mirela Imre Department of Animal Production and Veterinary Public Health, Faculty of Veterinary Medicine, Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” from Timisoara, Romania E-mail address: kalman [email protected] (K. Imre). Safe drinking water sources is a general concern within the European Union countries. Nowadays, water wells constitute a very important untreated potable water sources in many rural areas
of western Romania. However, they may become contaminated with different waterborne pathogens posing a risk for the human health. In this study, a total of 22 private household and community drinking water wells were investigated for the presence of “Giardia” spp. and “Cptosporidium” spp. Water samples were collected by microfiber filtration, concentrated in agreement with USEPA 1623 protocol, and screened for the pathogens presence through immunofluorescence microscopy and PCR techniques. Two samples (9.1%) showed positive results with both techniques for “Giardia”, but no “Cryptosporidium” was identified. The ruminant origin “G. duodenalis” assemblage E and the human origin sub-assemblage AII were recorded. The results indicate that the drinking water wells are potentially under the influence of other neighbor possible contaminated surface waters and can jeopardize the public health. Acknowledgements: This work was supported by a grant of the Romanian National Authority for Scientific Research and Innovation, CNCS – UEFISCDI, project number PN-II-RU-TE-2014-4-1300. http://dx.doi.org/10.1016/j.jbiotec.2017.06.1115 Bioimaging of modified bacterial nanocellulose using optical and electron microscopy Andrey Nikolaevich Agafonov 1,∗ , Marina Yurievna Yazykova 2 , Arina Ilinichna Sizova 2 1
Department of Nanoengineering, Samara National Research University, Samara, Russia 2 Department of Biochemistry, Biotechnology and Bioengineering, Samara National Research University, Samara, Russia E-mail address: [email protected] (A.N. Agafonov). Bacterial nanocellulose (BNC) is a natural product with high potential for biomedical application. As a novel wound dressing material, it needs appropriate modification to obtain antimicrobial activity. In the present study 6-O-metilsulfonilaminoatsetat and 6-O-tolilsulfonilaminoatsetat of BCN (product of bacteria Gluconacetobacter sucrofermentans B-11267) were preparated by modification of a cellulose ester and structure analysis to reveal alteration of micropores’ number from control sample was maid. Bright-field optical microscopy (Biomed-4) and scanning electron microscopy (FEI Quanta 200) techniques were used for these purposes. Control sample showed typical for BNC threedimensional netted composition with random arrangement of ribbon-like microfibrils 0.1–1 m. On its surface, cylindrical micropores 0.1–2 m apparent size with depth of lying down within 5 m also occurred. In spite of essential alterations in modified samples’ structure of microfibrils, stratified structure of the surface and micropores’ system were kept. However, number of micropores per unit area significantly decreased and was reduced by an order of magnitude in both modified samples. To come to conclusion, it could be explained by incorporation of functional groups during modification process. http://dx.doi.org/10.1016/j.jbiotec.2017.06.1116