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The effects of extracellular matrix components on the growth and migration of bovine retinal capillary endothelial cells and pericytes
228
Cell Biology
International
Reports,
Vol. 7 7, No. 3, March
1987
THE EFFECTS OF EXTRACELLULAR MATRIX COMPONENTS ON THE GROWTH AND MIGRATION OF BOVINE RETINAL CAPILLARY ENDOTHELIAL CELLS AND PERICYTES. L,C, McIntosh, L. Muckersie & J. V, Forrester. Department of Ophthalmology, University of Aberdeen. Angiogenesis occurs during many physiologocial and pathological tissue responses such as wound healing, tumorigenesis and retinal vascular diseases (especially diabetic retinopathy). Recent studies (Ungari et, al., 1985; Madri and Pratt, 1986; Shor et. al., 1986) have focused on the importance of the extracellular matrix in modulating endothelial cell behaviour. We have studied the effects of extracellular matrix components on the behaviour of bovine retinal capillary vascular cells. Retinal capillary endothelial cells but not pericytes require fibronectin for growth as a typical 'cobblestone' monolayer either on tissue culture plastic or on native (type I) reconstituted collagen Endothelial cell monolayers further develop gels. post-confluent two-dimensional loop structures on such matrices. Using a dye-binding growth assay (Laughton, 1984) we have shown that capillary endothelial cells and pericytes respond differently to retina-derived In addition, using a modified microgrowth factor. chemotaxis assay we have shown that capillary endothelial cells require fibronectin for migration whereas pericytes may migrate in its absence although fibroHeparin further nectin greatly facilitates movement. These results enhances these migratory responses. demonstrate the importance of extracellular tissue components in the angiogenic response in vitro and have -____ implications for --in vivo processes. This work Department, Blind.
was supported by the Scottish Home and Health, and the Royal National Institute for the
Analyt. Biochem., 140, 417 C. (1984) A. & Pratt, B. M. (1986) J. Histochem. Cytochem., 34, 85. Shor, A. M. & Shor, S. L. (1986) Microvaz. Res., 32,21 (1985) Invasion Metastasis, 2, 193. Ungari. S. et. al.,
Laughton, Madri, J.
Cell Biology
International
Reports,
Vol. 7 7, No. 3, March
1987
THE EFFECTS OF EXTRACELLULAR MATRIX COMPONENTS ON THE GROWTH AND MIGRATION OF BOVINE RETINAL CAPILLARY ENDOTHELIAL CELLS AND PERICYTES. L,C, McIntosh, L. Muckersie & J. V, Forrester. Department of Ophthalmology, University of Aberdeen. Angiogenesis occurs during many physiologocial and pathological tissue responses such as wound healing, tumorigenesis and retinal vascular diseases (especially diabetic retinopathy). Recent studies (Ungari et, al., 1985; Madri and Pratt, 1986; Shor et. al., 1986) have focused on the importance of the extracellular matrix in modulating endothelial cell behaviour. We have studied the effects of extracellular matrix components on the behaviour of bovine retinal capillary vascular cells. Retinal capillary endothelial cells but not pericytes require fibronectin for growth as a typical 'cobblestone' monolayer either on tissue culture plastic or on native (type I) reconstituted collagen Endothelial cell monolayers further develop gels. post-confluent two-dimensional loop structures on such matrices. Using a dye-binding growth assay (Laughton, 1984) we have shown that capillary endothelial cells and pericytes respond differently to retina-derived In addition, using a modified microgrowth factor. chemotaxis assay we have shown that capillary endothelial cells require fibronectin for migration whereas pericytes may migrate in its absence although fibroHeparin further nectin greatly facilitates movement. These results enhances these migratory responses. demonstrate the importance of extracellular tissue components in the angiogenic response in vitro and have -____ implications for --in vivo processes. This work Department, Blind.
was supported by the Scottish Home and Health, and the Royal National Institute for the
Analyt. Biochem., 140, 417 C. (1984) A. & Pratt, B. M. (1986) J. Histochem. Cytochem., 34, 85. Shor, A. M. & Shor, S. L. (1986) Microvaz. Res., 32,21 (1985) Invasion Metastasis, 2, 193. Ungari. S. et. al.,
Laughton, Madri, J.