LLOYD E. DONALDSON 712 Bentley Court, Tyler, TX. USA. 75703 Nineteen independent embryo transfer operators tested the efficacy of three superovulation regimens using a new FSH product (SUPER-OV@, Ausa Jntemational Inc. Tyler TX USA). Each operator was asked to use one or two treatment regimens at the recommended dose of 75 units per cow. The total dose was divided into equal parts and [l] three injections were admin&ered at 24 h intervals (3x1): [2] six injections were given at 12 h intervals (3x2) or [3] eight injections were given at 12 h intervals (4x2). Prostaglandin was given 48 h after the fit FSH injection to control ‘Ihe wperovulated cows classified as naval. There ~SlfUS. a large highly significant between operators 4.2 transferable/ total to The three regimens were at the level (By of data X,=(X+0.5)oJ, P 0.06 for embryos).
The mean
percent transferable 73f36%, mean fertilized 89+28% the fertilized degenerate The distribution the embryos was skewed. median transferable was 4 (10% of and the zero embryos of collections). the total recovered the was 7 (5.2% of and the zero embryos of collections). is one the frequency zero embryo after superovulation 533 cows F.S.H.P.’ (Schering Rahway NJ) at 30% 17% respectively transferable and embryos Il)onaldson, Theriogenology 22205-212 The best embryo collections with SUPER-OV@ 54155 and in another using a dose of units in 4x2 regimen. six batches SUPER-OV@ used this study (700,701,705,709,718,720) produced the same numbers of transferable embryos (5.8, 6.9, 6, 6.3, 5.4 and 5.7 respectively; by ANOVA of transformed data P = 0.401). It was concluded that the 3x1, 3x2 and 4x2 treatment regimens using SUPER-OV@ were effective in superovulating cattle when used under commercial conditions. There was a high percent transferable due to a high fertilization rate and a low embryo degeneration rate. There was no variation in the effectiveness of superovulation by six different batches of SUPER-OV@. The frequency of zero embryo collections was half the levels reported with F.S.H.-P.“.