- Email: [email protected]
The evaluation of mutagenic potential of some dental cements in used dentistry with salmonella Ames Microsome test and SOS chromotest
Poster presentations / Current Opinion in Biotechnology 24S (2013) S48–S143
mediate some of the genetic influence on normal skeletal growth and thus influence osteoporosis risk. The aim of the present study was to examine possible associations of T34C polymorphism in the CYP17 gene with femoral (F-BMD), spinal BMD (S-BMD), as well as circulating alkaline phosphatase (ALP), osteocalcin (OC; formation markers), beta-CrossLaps (CTx; resorption marker) in 350 Slovak postmenopausal women (62.26 ± 0.44 years). Women were selected according to strict inclusion criteria. Genetic polymorphism was detected by PCR-RFLP method. Genotype frequencies and frequencies of fractures were tested using the chi-square test. The differences of quantitative variables between the genotypes were analyzed by covariance analysis (GLM procedure) after correction of the measurements for age and BMI. The prevalence of each genotype was 30.9%, 49.0%, and 19.7% for TT, TC, and CC genotypes, respectively. No significant effect of the T34C polymorphism was recorded on femoral and spinal BMD, as well as on biochemical bone turnover markers in the tested population. However; heterozygous TC individuals had tendency to insignificantly lower F-BMD and S-BMD values with increasing OC and CTx concentrations which could indicate increased bone remodeling rate in this genotype group. The results can contribute to more detailed insight into the genetics of osteoporosis with respect to a population of Slovak postmenopausal women. Acknowledgement: The study was supported by the grant KEGA 035UKF-4/2013. http://dx.doi.org/10.1016/j.copbio.2013.05.297 The evaluation of mutagenic potential of some dental cements in used dentistry with salmonella Ames Microsome test and SOS chromotest Güven Uraz 1 , Baran Dadako˘glu 1 , Ebru Yılmaz 2 1 Gazi University, Faculty of Science, Department of Biology, Ankara, Turkey 2 Gazi University, Vocational School of Health Sciences, Ankara, Turkey E-mail address: [email protected] (G. Uraz).
Cements used in dentistry can bring important negative effects on human health due to their chemical structures. Chemicals can cause DNA damage and cancer cells. Various short-time tests are used to investigate DNA damage and cancer cells. Salmonella Ames Microsome test and SOS chromotest are commonly used shorttime tests. Three various cements with three different doses Cavex (zinc-oxide eugenol), Harvard (zinc phosphate), Dentsply Poly-FPlus (zinc-polycarboxylate) were used in this study. These cements were tested with both DMSO and artificial saliva which were used for solvent. Salmonella AMES Microsome test was used to identify mutagenic activity, SOS chromotest was used to identify genotoxic activity. According to the test results in DMSO, the tests using TA98 strain with S9 microsome enzyme, 40 mg doses of Poly-F-Plus and 13 mg doses of Harvard cements were not mutagenic but the other doses may have mutagenic effect. All doses of Cavex may have mutagenic effect. The tests using TA98 strain without S9 microsome enzyme and the test using TA100 strain w/wo S9 microsome enzyme, all doses of the all cements may have mutagenic effect. According to the test results in artificial saliva, the test using TA 98 strain w/wo s9 microsome enzyme all doses of Harvard, Poly-F Plus and 13 mg doses of Cavex cements may have mutagenic effect. The other doses of Cavex cement not identified due to inhibition. TA 100 strain w/wo s9 enzyme all doses of the Harvard, Poly-F Plus and Cavex siman may have a mutagenic effect. http://dx.doi.org/10.1016/j.copbio.2013.05.298
S99
Leptin increases ADAMTS9 gene expression in human chondrocytes Kursat Oguz Yaykasli 1 , Emine Yaykasli 2 , Mustafa Ozsahin 3 , Mustafa Uslu 4 , Ertugrul Kaya 5 1 Department of Medical Genetic, Medical Faculty, Duzce University, Duzce, Turkey 2 Department of Medical Biology and Genetic, Institute of Health Science, Duzce University, Duzce, Turkey 3 Department of Physical Medicine and Rehabilitation, Medical Faculty, Duzce University, Duzce, Turkey 4 Department of Orthopaedics and Traumatology, Medical Faculty, Duzce University, Duzce, Turkey 5 Department of Medical Pharmacology, Medical Faculty, Duzce University, Duzce, Turkey E-mail address: [email protected] (K.O. Yaykasli).
Rheumatoid arthritis (RA) is multifactorial and inflammatory disease. Even RA affects approximately 1% of world population, the etiology of RA is not elucidated in detail. ADAMTS9 is one of the aggrecanese, and known to be upregulated in inflammatory disease like RA. Leptin, the first characterized adipocytokines, was discovered in 1994. Leptin involves in several vital biological processes especially in immunity and inflammation. The elevated serum leptin level in RA was reported before. In this study, the potential role of leptin in etiology of RA was investigated. Recombinant human leptin was exposed to human primary cell culture at certain concentrations (100, 250, 500, 1000 ng/ml), and incubated for 12 hours and 24 hours. At the end of the incubation, RNA was extracted and 2 g of total RNA was reverse-transcribed by random primer. ADAMTS9 and actin gene expression were analyzed by Real-Time PCR method. The highest elevated ADAMTS9 gene expression was obtained at 1000 ng/ml dose for 24 hours. The irreversible destruction of extracellular matrix by aggrecanases like ADAMTS9 is key event of RA. The researchers focused on the causes of elevated expression level of aggrecanases in RA. Our study demonstrated that ADAMT9 gene expression was upregulated by leptin at 1000 ng/ml. Therefore, leptin with inflammatory properties may involve in the etiology of RA, but further investigations are needed to clarify in detail. http://dx.doi.org/10.1016/j.copbio.2013.05.299 Polymorphisms of TNF-alpha and coagulation genes in the etiopathogenesis of Sheehan’s Syndrome Nazife Tascioglu 1 , Elif Funda Sener 2 , Müge Gülcihan Onal 1 , Halit Diri 3 , Fahri Bayram 3 , Munis Dundar 1 1
Erciyes University, Medical School, Department of Medical Genetics, Kayseri, Turkey 2 Erciyes University, Medical School, Department of Medical Biology, Kayseri, Turkey 3 Erciyes University, Medical School, Department of Endocrinology, Kayseri, Turkey E-mail address: [email protected] (M.G. Onal). Sheehan’s Syndrome (SS) is the pituitary hormone deficiency which develops after severe postpartum hemorrhage. As the main pathology that leads to SS is ischemic pituitary necrosis, disorders of coagulation seem to be the main contributing factor in the etiopathogenesis. In addition, pituitary autoantibodies are present in many patients and thereby TNF-␣ gene may also play a role. We aimed to investigate FII (G20210A), FV (G1691A), MTHFR (C677T and A1298C), and TNF-␣ (−308 G>A) gene polymorphisms in patients with SS. Peripheral blood samples were obtained from
mediate some of the genetic influence on normal skeletal growth and thus influence osteoporosis risk. The aim of the present study was to examine possible associations of T34C polymorphism in the CYP17 gene with femoral (F-BMD), spinal BMD (S-BMD), as well as circulating alkaline phosphatase (ALP), osteocalcin (OC; formation markers), beta-CrossLaps (CTx; resorption marker) in 350 Slovak postmenopausal women (62.26 ± 0.44 years). Women were selected according to strict inclusion criteria. Genetic polymorphism was detected by PCR-RFLP method. Genotype frequencies and frequencies of fractures were tested using the chi-square test. The differences of quantitative variables between the genotypes were analyzed by covariance analysis (GLM procedure) after correction of the measurements for age and BMI. The prevalence of each genotype was 30.9%, 49.0%, and 19.7% for TT, TC, and CC genotypes, respectively. No significant effect of the T34C polymorphism was recorded on femoral and spinal BMD, as well as on biochemical bone turnover markers in the tested population. However; heterozygous TC individuals had tendency to insignificantly lower F-BMD and S-BMD values with increasing OC and CTx concentrations which could indicate increased bone remodeling rate in this genotype group. The results can contribute to more detailed insight into the genetics of osteoporosis with respect to a population of Slovak postmenopausal women. Acknowledgement: The study was supported by the grant KEGA 035UKF-4/2013. http://dx.doi.org/10.1016/j.copbio.2013.05.297 The evaluation of mutagenic potential of some dental cements in used dentistry with salmonella Ames Microsome test and SOS chromotest Güven Uraz 1 , Baran Dadako˘glu 1 , Ebru Yılmaz 2 1 Gazi University, Faculty of Science, Department of Biology, Ankara, Turkey 2 Gazi University, Vocational School of Health Sciences, Ankara, Turkey E-mail address: [email protected] (G. Uraz).
Cements used in dentistry can bring important negative effects on human health due to their chemical structures. Chemicals can cause DNA damage and cancer cells. Various short-time tests are used to investigate DNA damage and cancer cells. Salmonella Ames Microsome test and SOS chromotest are commonly used shorttime tests. Three various cements with three different doses Cavex (zinc-oxide eugenol), Harvard (zinc phosphate), Dentsply Poly-FPlus (zinc-polycarboxylate) were used in this study. These cements were tested with both DMSO and artificial saliva which were used for solvent. Salmonella AMES Microsome test was used to identify mutagenic activity, SOS chromotest was used to identify genotoxic activity. According to the test results in DMSO, the tests using TA98 strain with S9 microsome enzyme, 40 mg doses of Poly-F-Plus and 13 mg doses of Harvard cements were not mutagenic but the other doses may have mutagenic effect. All doses of Cavex may have mutagenic effect. The tests using TA98 strain without S9 microsome enzyme and the test using TA100 strain w/wo S9 microsome enzyme, all doses of the all cements may have mutagenic effect. According to the test results in artificial saliva, the test using TA 98 strain w/wo s9 microsome enzyme all doses of Harvard, Poly-F Plus and 13 mg doses of Cavex cements may have mutagenic effect. The other doses of Cavex cement not identified due to inhibition. TA 100 strain w/wo s9 enzyme all doses of the Harvard, Poly-F Plus and Cavex siman may have a mutagenic effect. http://dx.doi.org/10.1016/j.copbio.2013.05.298
S99
Leptin increases ADAMTS9 gene expression in human chondrocytes Kursat Oguz Yaykasli 1 , Emine Yaykasli 2 , Mustafa Ozsahin 3 , Mustafa Uslu 4 , Ertugrul Kaya 5 1 Department of Medical Genetic, Medical Faculty, Duzce University, Duzce, Turkey 2 Department of Medical Biology and Genetic, Institute of Health Science, Duzce University, Duzce, Turkey 3 Department of Physical Medicine and Rehabilitation, Medical Faculty, Duzce University, Duzce, Turkey 4 Department of Orthopaedics and Traumatology, Medical Faculty, Duzce University, Duzce, Turkey 5 Department of Medical Pharmacology, Medical Faculty, Duzce University, Duzce, Turkey E-mail address: [email protected] (K.O. Yaykasli).
Rheumatoid arthritis (RA) is multifactorial and inflammatory disease. Even RA affects approximately 1% of world population, the etiology of RA is not elucidated in detail. ADAMTS9 is one of the aggrecanese, and known to be upregulated in inflammatory disease like RA. Leptin, the first characterized adipocytokines, was discovered in 1994. Leptin involves in several vital biological processes especially in immunity and inflammation. The elevated serum leptin level in RA was reported before. In this study, the potential role of leptin in etiology of RA was investigated. Recombinant human leptin was exposed to human primary cell culture at certain concentrations (100, 250, 500, 1000 ng/ml), and incubated for 12 hours and 24 hours. At the end of the incubation, RNA was extracted and 2 g of total RNA was reverse-transcribed by random primer. ADAMTS9 and actin gene expression were analyzed by Real-Time PCR method. The highest elevated ADAMTS9 gene expression was obtained at 1000 ng/ml dose for 24 hours. The irreversible destruction of extracellular matrix by aggrecanases like ADAMTS9 is key event of RA. The researchers focused on the causes of elevated expression level of aggrecanases in RA. Our study demonstrated that ADAMT9 gene expression was upregulated by leptin at 1000 ng/ml. Therefore, leptin with inflammatory properties may involve in the etiology of RA, but further investigations are needed to clarify in detail. http://dx.doi.org/10.1016/j.copbio.2013.05.299 Polymorphisms of TNF-alpha and coagulation genes in the etiopathogenesis of Sheehan’s Syndrome Nazife Tascioglu 1 , Elif Funda Sener 2 , Müge Gülcihan Onal 1 , Halit Diri 3 , Fahri Bayram 3 , Munis Dundar 1 1
Erciyes University, Medical School, Department of Medical Genetics, Kayseri, Turkey 2 Erciyes University, Medical School, Department of Medical Biology, Kayseri, Turkey 3 Erciyes University, Medical School, Department of Endocrinology, Kayseri, Turkey E-mail address: [email protected] (M.G. Onal). Sheehan’s Syndrome (SS) is the pituitary hormone deficiency which develops after severe postpartum hemorrhage. As the main pathology that leads to SS is ischemic pituitary necrosis, disorders of coagulation seem to be the main contributing factor in the etiopathogenesis. In addition, pituitary autoantibodies are present in many patients and thereby TNF-␣ gene may also play a role. We aimed to investigate FII (G20210A), FV (G1691A), MTHFR (C677T and A1298C), and TNF-␣ (−308 G>A) gene polymorphisms in patients with SS. Peripheral blood samples were obtained from