- Email: [email protected]
The expression of natural cytotoxicity receptors and cytokines production by NK cells for uterine endometrium and peripheral blood in infertile women
68
Abstracts of poster session / Journal of Reproductive Immunology 115 (2016) 56–70
P1-41 The efficacy of paternal lymphocytes immunizations in patients with recurrent miscarriages and the presence of autoantibodies ˛ Hanna Motak-Pochrzest, Andrzej Malinowski The Opole University of Technology, Faculty of Physical Education and Physiotherapy, Opole, Poland Object: The objective of this study was to evaluate the efficacy of paternal lymphocytes immunizations (PLI) in patients with RM and the presence of autoantibodies. Materials and methods: 227 patients with a history of 3 or more RM were evaluated. None of the participants had autoimmune disease. Patients with previously found autoantibodies (ACA, 2GPI, LA, ATA and ANA) were included after miscarriage despite treatment during pregnancies. All patients were qualified on the basis of the negative lymphocytotoxic test (LCT) and lack of lymphocyte activity in mixed lymphocyte reaction test (MLR). Results: In the group of 227 women who received PLI, 189 pregnancies resulted in term delivery (success rate = 83.3%). According to the incidence of autoantibodies we have observed success rates of pregnancies in: 42 patients with the presence of ANA (84%), 57 patients with the presence of APL (71.25%)and 38 patients with the presence of ATA (79.1%). The presence of ANA and low titers of APL did not influence the efficacy of PLI (p > 0.01). Medium or high titers of APL significantly reduced the rate of successful pregnancies by 61.9% (p < 0.01) after PLI. The presence of ANA in the sera was not a risk factor for the subsequent pregnancy. Conclusions: The results of our studies suggest that this treatment performed according to an appropriate immunization protocol was demonstrated to be safe and effective in RM patients irrespective of the presence of autoantibodies. http://dx.doi.org/10.1016/j.jri.2016.04.212 P1-42 The expression of natural cytotoxicity receptors and cytokines production by NK cells for uterine endometrium and peripheral blood in infertile women Atsushi Fukui, Kohei Fuchinoue, Hitomi Chiba, Ayano Funamizu, Ayako Taima, Mai Kamoi, Hideki Mizunuma Hirosaki University Graduate School of Medicine, Japan Objectives: Most abundant immune cell at secretory phase to early pregnancy in the uterus is NK cell. GM-CSF is produced by NK cell, T cell and so on and is important for the embryo quality and growth, and uterine endometrial environment. Recently, GM-CSF added medium is available in IVF-ET. The aim of this study is to know the function of NK cells by the evaluation of those surface antigen and cytokines production and clinical outcome of IVF-ET. Methods: Peripheral blood and uterine endometrium were obtained at the midsecretory phase prior to IVF-ET. The expression of NKp46 and CD16 (CD56dim and CD56bright) and the production of cytokines (GM-CSF, TNF-␣, IFN-␥, IL-4 and IL-10) by NK cells were analyzed using flow cytometry. Results: Endometrial CD56dim/NKp46+ cells were significantly lower in IVF-ET failed group (n = 14) compared with implanted group (n = 19, p < 0.05). Endometrial TNF-␣ producing NK cells were significantly higher in failed group compared with implanted group
(p < 0.05). In implanted group, endometrial CD56bright/NKp46+ (p < 0.05) cells and IFN-␥ (p < 0.05) producing NK cells were significantly lower in aborted group (n = 5) compared with ongoing group (n = 9). There was a significant positive correlation in the percentage of GM-CSF producing NK cells between peripheral blood and endometrium (r2 = 0.379, p < 0.01). Conclusions: Peripheral blood and endometrial NK cell may have important roles for the achievement and maintenance of pregnancy. http://dx.doi.org/10.1016/j.jri.2016.04.213 P1-43 The status of sperm immobilizing-antibody (SI-Ab) detected in infertile females (therapy and pathogenic mechanisms) Akiko Hasegawa, Hardiyanto Lutfi, Minoru Shigeta, Hiroaki Shibahara Hyogo College of Medicine, Japan WHO guidelines state that anti-sperm antibodies (ASAs) may be found in cases of male infertility associated with sperm impairment, because sperm antigens are isolated from the immune system by the blood-testes barrier. Females may also produce ASAs after exposure to sperm, which contain foreign antigens for females. Most ASAs in female patients’ sera which were detected by ordinary methods did not show harmful effects on sperm. ASAs which were detected by a complement-dependent sperm immobilization test (termed SI-Abs), however, significantly correlated to infertility. This suggested that ASAs caused tremendous damage to sperm when involved in complement activation. Since SI-Abs were reported to be a plausible pathogenic factor, many physicians and researchers have been investigating treatments and mechanisms for producing SI-Abs. Recently, laboratory data for SI-Abs has become available from several testing companies. Following a flow diagram we have suggested, suitable treatments for individuals can be chosen based on the data. Furthermore, our scientific analysis revealed that one of the SI-Abs reacted to CD52 in male reproductive tissues (mrt-CD52, which is a glycosyl-phosphatidyl-inositol anchor protein having a complement-suppressive function). Taken together, we concluded that the sperm impairment caused by the SI-Ab was associated with inhibition of the complementsuppressive activity of mrt-CD52. http://dx.doi.org/10.1016/j.jri.2016.04.214 P1-44 Transcriptomic analysis – Cytokine micropattern and novel biomarker genes in spermatogenic arrests Maciej Kurpisz, Agnieszka Malcher, Natalia Rozwadowska, Piotr Jedrzejczak Institute of Human Genetics, Pol. Acad. Sci. Poznan, Poland Genes of immunological system may act as predictive factor for azoospermia molecular diagnosis, treatment and monitoring. We have analyzed 20 testicular biopsy samples with Affymetrix Human Gene 1.0 ST microarrays – 16 were obtained from patients various types of NOA and four were with normal spermatogenesis. Six of the patients with NOA syndrome were subjected to gonadotropin therapy (hCG+rFSH) and one positive re-analyzed in microarrays before and (one case) after successful treatment. Genes
Abstracts of poster session / Journal of Reproductive Immunology 115 (2016) 56–70
P1-41 The efficacy of paternal lymphocytes immunizations in patients with recurrent miscarriages and the presence of autoantibodies ˛ Hanna Motak-Pochrzest, Andrzej Malinowski The Opole University of Technology, Faculty of Physical Education and Physiotherapy, Opole, Poland Object: The objective of this study was to evaluate the efficacy of paternal lymphocytes immunizations (PLI) in patients with RM and the presence of autoantibodies. Materials and methods: 227 patients with a history of 3 or more RM were evaluated. None of the participants had autoimmune disease. Patients with previously found autoantibodies (ACA, 2GPI, LA, ATA and ANA) were included after miscarriage despite treatment during pregnancies. All patients were qualified on the basis of the negative lymphocytotoxic test (LCT) and lack of lymphocyte activity in mixed lymphocyte reaction test (MLR). Results: In the group of 227 women who received PLI, 189 pregnancies resulted in term delivery (success rate = 83.3%). According to the incidence of autoantibodies we have observed success rates of pregnancies in: 42 patients with the presence of ANA (84%), 57 patients with the presence of APL (71.25%)and 38 patients with the presence of ATA (79.1%). The presence of ANA and low titers of APL did not influence the efficacy of PLI (p > 0.01). Medium or high titers of APL significantly reduced the rate of successful pregnancies by 61.9% (p < 0.01) after PLI. The presence of ANA in the sera was not a risk factor for the subsequent pregnancy. Conclusions: The results of our studies suggest that this treatment performed according to an appropriate immunization protocol was demonstrated to be safe and effective in RM patients irrespective of the presence of autoantibodies. http://dx.doi.org/10.1016/j.jri.2016.04.212 P1-42 The expression of natural cytotoxicity receptors and cytokines production by NK cells for uterine endometrium and peripheral blood in infertile women Atsushi Fukui, Kohei Fuchinoue, Hitomi Chiba, Ayano Funamizu, Ayako Taima, Mai Kamoi, Hideki Mizunuma Hirosaki University Graduate School of Medicine, Japan Objectives: Most abundant immune cell at secretory phase to early pregnancy in the uterus is NK cell. GM-CSF is produced by NK cell, T cell and so on and is important for the embryo quality and growth, and uterine endometrial environment. Recently, GM-CSF added medium is available in IVF-ET. The aim of this study is to know the function of NK cells by the evaluation of those surface antigen and cytokines production and clinical outcome of IVF-ET. Methods: Peripheral blood and uterine endometrium were obtained at the midsecretory phase prior to IVF-ET. The expression of NKp46 and CD16 (CD56dim and CD56bright) and the production of cytokines (GM-CSF, TNF-␣, IFN-␥, IL-4 and IL-10) by NK cells were analyzed using flow cytometry. Results: Endometrial CD56dim/NKp46+ cells were significantly lower in IVF-ET failed group (n = 14) compared with implanted group (n = 19, p < 0.05). Endometrial TNF-␣ producing NK cells were significantly higher in failed group compared with implanted group
(p < 0.05). In implanted group, endometrial CD56bright/NKp46+ (p < 0.05) cells and IFN-␥ (p < 0.05) producing NK cells were significantly lower in aborted group (n = 5) compared with ongoing group (n = 9). There was a significant positive correlation in the percentage of GM-CSF producing NK cells between peripheral blood and endometrium (r2 = 0.379, p < 0.01). Conclusions: Peripheral blood and endometrial NK cell may have important roles for the achievement and maintenance of pregnancy. http://dx.doi.org/10.1016/j.jri.2016.04.213 P1-43 The status of sperm immobilizing-antibody (SI-Ab) detected in infertile females (therapy and pathogenic mechanisms) Akiko Hasegawa, Hardiyanto Lutfi, Minoru Shigeta, Hiroaki Shibahara Hyogo College of Medicine, Japan WHO guidelines state that anti-sperm antibodies (ASAs) may be found in cases of male infertility associated with sperm impairment, because sperm antigens are isolated from the immune system by the blood-testes barrier. Females may also produce ASAs after exposure to sperm, which contain foreign antigens for females. Most ASAs in female patients’ sera which were detected by ordinary methods did not show harmful effects on sperm. ASAs which were detected by a complement-dependent sperm immobilization test (termed SI-Abs), however, significantly correlated to infertility. This suggested that ASAs caused tremendous damage to sperm when involved in complement activation. Since SI-Abs were reported to be a plausible pathogenic factor, many physicians and researchers have been investigating treatments and mechanisms for producing SI-Abs. Recently, laboratory data for SI-Abs has become available from several testing companies. Following a flow diagram we have suggested, suitable treatments for individuals can be chosen based on the data. Furthermore, our scientific analysis revealed that one of the SI-Abs reacted to CD52 in male reproductive tissues (mrt-CD52, which is a glycosyl-phosphatidyl-inositol anchor protein having a complement-suppressive function). Taken together, we concluded that the sperm impairment caused by the SI-Ab was associated with inhibition of the complementsuppressive activity of mrt-CD52. http://dx.doi.org/10.1016/j.jri.2016.04.214 P1-44 Transcriptomic analysis – Cytokine micropattern and novel biomarker genes in spermatogenic arrests Maciej Kurpisz, Agnieszka Malcher, Natalia Rozwadowska, Piotr Jedrzejczak Institute of Human Genetics, Pol. Acad. Sci. Poznan, Poland Genes of immunological system may act as predictive factor for azoospermia molecular diagnosis, treatment and monitoring. We have analyzed 20 testicular biopsy samples with Affymetrix Human Gene 1.0 ST microarrays – 16 were obtained from patients various types of NOA and four were with normal spermatogenesis. Six of the patients with NOA syndrome were subjected to gonadotropin therapy (hCG+rFSH) and one positive re-analyzed in microarrays before and (one case) after successful treatment. Genes