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The First Case of Canine Strongyloides stercoralis Infection in Finland
284
ESVP/ECVP Proceedings 2009
141:4, 2009
25
26
LYMPHANGIECTATIC FIBROUS POLYP OF THE TONSIL IN A DOG A.-L. Bauchet, E. Balme, J.-L. Thibault, J.-J. Fontaine and N. Cordonnier Department of Pathology, Veterinary School of Alfort, 7 avenue du Ge´ne´ral de Gaulle, 94704 Maisons-Alfort, France Introduction: In dogs, the most frequent tonsillar lesions are tonsillitis, lymphoid hyperplasia and malignant neoplasms, especially squamous cell carcinoma and lymphoma. Benign tonsillar proliferations such as inflammatory polyps are uncommon. In man, several benign and malignant lesions of tonsils are reported, including a rare entity, the lymphangiectatic fibrous polyp. We report a case of a single tonsillar polyp in a 13-year-old female Bichon dog with moderate inspiratory dyspnoea associated with a pedunculated pink mass growing from the left palatine tonsil and partially obstructing the pharyngeal lumen. Materials and Methods: Tissue specimens were prepared for histology by standard protocols. Immunohistochemistry was performed using the streptavidin-biotin-complex method and anti-human LYVE-1 and CD3 antibodies. Results: Histologically, the polyp was covered by a non-keratinized pluristratified squamous epithelium and was formed by several lymphoid follicles and a central core of numerous severely ectatic lymphatic vessels. Endothelial cells lining the dilated vessels labelled positively for LYVE-1, an antigen specific for lymphatic endothelial cells. Conclusions: We describe a case of an unusual tonsillar polyp in a dog sharing strong clinical and histological similarities with the rare human lymphangiectatic fibrous polyp.
THE INFLUENCE OF PUFA ON NUMBERS OF CUTANEOUS MAST CELLS AND EOSINOPHILS IN DOGS WITH ATOPIC DERMATITIS D. Denk *, W. Hermanns *, S. Bettenay y and R. Mueller* *Ludwig-Maximilians-University, Munich, Germany and yColorado State University, Colorado, USA Introduction: Canine atopic dermatitis (AD) constitutes an important skin disease commonly seen in veterinary practice. Treatment is multifaceted and often involves polyunsaturated fatty acid (PUFA) supplementation. The aim of this double-blinded, randomized, placebo-controlled study was to assess the influence of PUFA on inflammatory cell infiltration of the skin. Materials and Methods: Twenty-eight dogs with AD were assigned to one of three groups and supplemented for 10 weeks with either one of two fatty acid products or a placebo. Skin biopsies were taken prior to and at the end of the trial. Clinical signs and pruritus were evaluated. The number of mast cells (MCs) and eosinophils (ELs) per mm2 was quantified. Results: There was no significant difference between cell counts before and after the study, nor when comparing groups amongst themselves. Positive correlations were observed between cell counts and pruritus and between cell counts and clinical signs. Conclusions: The long survival time of inactive inflammatory cells and the relatively short study period may account for these findings. Since only MCs and ELs were evaluated, a decline in other cell populations involved in AD cannot be discounted. The observed positive correlations provide further evidence that PUFAs influence mediators of effector cells.
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THE FIRST CASE OF CANINE STRONGYLOIDES STERCORALIS INFECTION IN FINLAND K. Dillard *, S. Saari y and M. Anttila* *Finnish Food Safety Authority Evira, Pathology Unit, Helsinki and y University of Helsinki, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Helsinki, Finland Introduction: Strongyloides stercoralis is a small intestinal threadworm of dogs, cats and primates. It is mostly encountered in tropical and subtropical countries. Materials and Methods: A 10-week-old Yorkshire terrier puppy was submitted for necropsy examination after 3 weeks of intermittent diarrhoea, vomiting and pain on defecation followed by sudden death. The puppy was born and raised in a Finnish kennel. Samples for histology were collected during necropsy and processed routinely and stained with haematoxylin and eosin. Faecal samples from the other dogs in the kennel were examined using the Baerman method and collected nematodes were examined by light microscopy and scanning electron microscopy. Results: Numerous intramucosal nematodes and larvae with associated moderate lymphoplasmacytic infiltrates were located in the small intestine. The adult nematodes were 2 e 2.5 mm long and had a rhabditiform oesophagus, paired genital tract, platymyrian meromyrian musculature, an intestine composed of uninucleate cells and a hexagonal mouth with six papillae. The larvae had a prominent genital primordium. Conclusions: This case shows that Strongyloides stercoralis can complete its life cycle and cause disease in dogs in Northern Europe.
MODIFICATIONS OF VISCERAL LYMPHOID TISSUE INDUCED IN CHICKENS BY AN ORALLY DELIVERED LECTIN G. Dinescu, A. Pop, I. Togoe, M. Militaru, E. Ciobotaru and T. Soare Faculty of Veterinary Medicine Bucharest, Romania Introduction: Lectins are carbohydrate-binding proteins and are described in almost all living organisms. They are involved in many biological processes, including immunomodulation. Some plant lectins are also known as antinutrients due to their pathological effects on the mucosa of the gastrointestinal tract. The aim of the present study was to evaluate the morphological changes in chickens following oral administration of potato shoot lectin. Materials and Methods: A potato shoot lectin preparation was obtained by affinity chromatography and administered in the drinking water to 3-day-old chickens for 7 days. The chickens were then killed and submitted for necropsy examination. Samples of intestine, kidney, spleen, liver and myocardium were processed for histopathological evaluation with Masson’s trichrome stain. Results: No gross lesions were detected. Increased reactivity of gutassociated lymphoid tissue and of the visceral lymphoid tissue of the liver was noticed in all treated chickens. The presence of visceral lymphoid nodules in the myocardium was an unusual finding. Conclusions: Administration of potato shoot lectin to chickens stimulates the formation of visceral lymphoid nodules, especially in the liver and myocardium.
ESVP/ECVP Proceedings 2009
141:4, 2009
25
26
LYMPHANGIECTATIC FIBROUS POLYP OF THE TONSIL IN A DOG A.-L. Bauchet, E. Balme, J.-L. Thibault, J.-J. Fontaine and N. Cordonnier Department of Pathology, Veterinary School of Alfort, 7 avenue du Ge´ne´ral de Gaulle, 94704 Maisons-Alfort, France Introduction: In dogs, the most frequent tonsillar lesions are tonsillitis, lymphoid hyperplasia and malignant neoplasms, especially squamous cell carcinoma and lymphoma. Benign tonsillar proliferations such as inflammatory polyps are uncommon. In man, several benign and malignant lesions of tonsils are reported, including a rare entity, the lymphangiectatic fibrous polyp. We report a case of a single tonsillar polyp in a 13-year-old female Bichon dog with moderate inspiratory dyspnoea associated with a pedunculated pink mass growing from the left palatine tonsil and partially obstructing the pharyngeal lumen. Materials and Methods: Tissue specimens were prepared for histology by standard protocols. Immunohistochemistry was performed using the streptavidin-biotin-complex method and anti-human LYVE-1 and CD3 antibodies. Results: Histologically, the polyp was covered by a non-keratinized pluristratified squamous epithelium and was formed by several lymphoid follicles and a central core of numerous severely ectatic lymphatic vessels. Endothelial cells lining the dilated vessels labelled positively for LYVE-1, an antigen specific for lymphatic endothelial cells. Conclusions: We describe a case of an unusual tonsillar polyp in a dog sharing strong clinical and histological similarities with the rare human lymphangiectatic fibrous polyp.
THE INFLUENCE OF PUFA ON NUMBERS OF CUTANEOUS MAST CELLS AND EOSINOPHILS IN DOGS WITH ATOPIC DERMATITIS D. Denk *, W. Hermanns *, S. Bettenay y and R. Mueller* *Ludwig-Maximilians-University, Munich, Germany and yColorado State University, Colorado, USA Introduction: Canine atopic dermatitis (AD) constitutes an important skin disease commonly seen in veterinary practice. Treatment is multifaceted and often involves polyunsaturated fatty acid (PUFA) supplementation. The aim of this double-blinded, randomized, placebo-controlled study was to assess the influence of PUFA on inflammatory cell infiltration of the skin. Materials and Methods: Twenty-eight dogs with AD were assigned to one of three groups and supplemented for 10 weeks with either one of two fatty acid products or a placebo. Skin biopsies were taken prior to and at the end of the trial. Clinical signs and pruritus were evaluated. The number of mast cells (MCs) and eosinophils (ELs) per mm2 was quantified. Results: There was no significant difference between cell counts before and after the study, nor when comparing groups amongst themselves. Positive correlations were observed between cell counts and pruritus and between cell counts and clinical signs. Conclusions: The long survival time of inactive inflammatory cells and the relatively short study period may account for these findings. Since only MCs and ELs were evaluated, a decline in other cell populations involved in AD cannot be discounted. The observed positive correlations provide further evidence that PUFAs influence mediators of effector cells.
27
28
THE FIRST CASE OF CANINE STRONGYLOIDES STERCORALIS INFECTION IN FINLAND K. Dillard *, S. Saari y and M. Anttila* *Finnish Food Safety Authority Evira, Pathology Unit, Helsinki and y University of Helsinki, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Helsinki, Finland Introduction: Strongyloides stercoralis is a small intestinal threadworm of dogs, cats and primates. It is mostly encountered in tropical and subtropical countries. Materials and Methods: A 10-week-old Yorkshire terrier puppy was submitted for necropsy examination after 3 weeks of intermittent diarrhoea, vomiting and pain on defecation followed by sudden death. The puppy was born and raised in a Finnish kennel. Samples for histology were collected during necropsy and processed routinely and stained with haematoxylin and eosin. Faecal samples from the other dogs in the kennel were examined using the Baerman method and collected nematodes were examined by light microscopy and scanning electron microscopy. Results: Numerous intramucosal nematodes and larvae with associated moderate lymphoplasmacytic infiltrates were located in the small intestine. The adult nematodes were 2 e 2.5 mm long and had a rhabditiform oesophagus, paired genital tract, platymyrian meromyrian musculature, an intestine composed of uninucleate cells and a hexagonal mouth with six papillae. The larvae had a prominent genital primordium. Conclusions: This case shows that Strongyloides stercoralis can complete its life cycle and cause disease in dogs in Northern Europe.
MODIFICATIONS OF VISCERAL LYMPHOID TISSUE INDUCED IN CHICKENS BY AN ORALLY DELIVERED LECTIN G. Dinescu, A. Pop, I. Togoe, M. Militaru, E. Ciobotaru and T. Soare Faculty of Veterinary Medicine Bucharest, Romania Introduction: Lectins are carbohydrate-binding proteins and are described in almost all living organisms. They are involved in many biological processes, including immunomodulation. Some plant lectins are also known as antinutrients due to their pathological effects on the mucosa of the gastrointestinal tract. The aim of the present study was to evaluate the morphological changes in chickens following oral administration of potato shoot lectin. Materials and Methods: A potato shoot lectin preparation was obtained by affinity chromatography and administered in the drinking water to 3-day-old chickens for 7 days. The chickens were then killed and submitted for necropsy examination. Samples of intestine, kidney, spleen, liver and myocardium were processed for histopathological evaluation with Masson’s trichrome stain. Results: No gross lesions were detected. Increased reactivity of gutassociated lymphoid tissue and of the visceral lymphoid tissue of the liver was noticed in all treated chickens. The presence of visceral lymphoid nodules in the myocardium was an unusual finding. Conclusions: Administration of potato shoot lectin to chickens stimulates the formation of visceral lymphoid nodules, especially in the liver and myocardium.