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MSP1 polymorphism in primary hyperlipidemia patients and control group
S184
Abstracts
distribution and allelic frequencies of polymorphism were determined and compared in primary hyperlipidemic and normolipidemic subjects. Result: The results showed that activity of plasma cholesterol ester transfer was significantly higher in primary hyperlipidemic than in controls (p = 0.001). The genotype and allelic frequencies for this polymorphism differed significantly between primary hyperlipidemic patients and controls (p = 0.022 and p = 0.039, respectively). Conclusion: The genotype and allelic frequencies for D442G polymorphism of cholesterol ester transfer protein gene were statistically different compared to control. Keywords: D442G polymorphism, Cholesterol ester transfer protein activity, Iranian population doi:10.1016/j.clinbiochem.2011.08.946
Poster — [A-10-1229-2] The genotype and allelic frequencies of CETP/MSP1 polymorphism in primary hyperlipidemia patients and control group Hassanzadeh Taghia, Saidijam Massoudb, Latifi Zeinaba, Paoli Maxc a Research Center for Molecular Medicine, Hamadan University of Medical Science, Hamadan, Iran b Molecular Medicine and Genetics Department, School of Medicine, Hamadan University of Medical Science, Hamadan, Iran c School of Pharmacy and Center for Biomolecular Science, University of Nottingham, Nottingham, UK E-mail addresses: [email protected] (H. Taghi), [email protected] (S. Massoud), [email protected] (L. Zeinab), [email protected] (P. Max) Introduction: Primary hyperlipidemia is considered to be major risk factor for pancreatitis, atherosclerosis and coronary heart disease. Gene polymorphism of cholesterol ester transfer protein is known to be associated with changes in lipid levels. Method: Polymerase chain reaction and restriction fragment length polymorphisms were used for genotype detection. Genotype distribution and allelic frequencies of polymorphism were determined and compared in primary hyperlipidemia and normolipidemic subjects. Result: The results showed that activity of plasma cholesterol ester transfer was significantly higher in primary hyperlipidemic than in controls (p = 0.001). The genotype and allelic frequencies for this polymorphism were not statistically different between primary hyperlipidemic patients and controls. Conclusion: The genotype and allelic frequencies for MSP1 polymorphism of cholesterol ester transfer protein gene were not statistically different compared to control. Keywords: MSP1 polymorphism, Cholesterol ester transfer protein activity, Iranian population
doi:10.1016/j.clinbiochem.2011.08.947
Oral — [A-10-1229-3] Effect of cholesteryl ester transfer protein MSP1 polymorphism on serum lipid levels and CETP activity in Iranian primary hyperlipidemic patients Hassanzadeh Taghia, Saidijam Massoudb, Latifi Zeinaba, Paoli Maxc a Research Center for Molecular Medicine, Hamadan University of Medical Science, Hamadan, Iran
b
Molecular Medicine and Genetics Department, School of Medicine, Hamadan University of Medical Science, Hamadan, Iran c School of Pharmacy and Center for Biomolecular Science, University of Nottingham, Nottingham, UK E-mail addresses: [email protected] (H. Taghi), [email protected] (S. Massoud), [email protected] (L. Zeinab), [email protected] (P. Max) Introduction: Cholesteryl ester transfer protein (CETP) gene polymorphism is known to be associated with changes in lipid profiles. Primary hyperlipidaemia is considered to be a major risk factor for pancreatitis, atherosclerosis and coronary heart disease. We studied the effects of MSPI polymorphism of cholesteryl ester transfer on serum lipid levels and CETP activity in Iranian primary hyperlipidemic patients. Methods: This study included 316 Iranian individuals (102 primary hyperlipidaemic patients and 214 healthycontrol). Polymerase chain reaction and restriction fragment length polymorphisms were used for genotype detection. To determine the relationship between MSP1 polymorphism and lipid levels, lipids and CETP activity were measured in primary hyperlipidemic and normolipidaemic subjects, with and without MSP1 polymorphism. Results: Plasma CETP activity was significantly (P< 0.001) higher in primary hyperlipidemic individuals than in controls. Plasma HDL-C was higher in both groups, in the AA genotype than in the GA and GG genotypes, whereas the serum CETP activity was lower in AA genotype compared with other genotypes (GA and GG). In addition Plasma TC was higher in both groups, in the AA genotype than in the GA genotypes. Conclusions: The results showed that MSP1 polymorphism of CETP gene was associated with changes in lipids profile and plasma CETP activity in the selected population and might have a role in contributing to genetic risk of developing coronary artery disease. Keywords: Cholesteryl ester transfer protein, Iranian population, Primary hyperlipidaemia, MSP1 polymorphism doi:10.1016/j.clinbiochem.2011.08.948
Poster — [A-10-1240-1] Investigation about the quantity of reduction the generation time of Cl. novyi (type B) vaccinal strain bacteria (infectious necrotic Hepatitis factor), with increasing various quantity of vitamin solution to culture Lalalizadeh Samaneh Mashhad, Islamic Republic of Iran E-mail address: [email protected] Introduction: Type B Novyi species belongs to clostridia. That is gram-positive and anaerobic which is able to make α fatal toxin. Because of the toxin, it is very important to produce vaccine against the disease. This research tried to reduce the generation time in order to make pure toxin to obtain high quality vaccine. Method: At first we must make culture and then add vitamin solution (biotin, nicotin amid, B1, B12, B6) in order to prepare growing process. Then we incubate foregoing culture for 24 h to control and avoid it from being contaminated. After that vaccinal strain of Cl. novyi (type B) was inoculated in it. At the end, we used the produced toxin to determine MLD after passing 24 h. Results and conclusion: Observations showed that vitamin solution added to culture caused reduction of generation time from 72 to 24 h. In the reduction period (72–24 = 48 h), MLD was promoted, thus, produced toxin had better quality. These observations are very important for making high quality vaccine. In many researches, the role of various vitamins on reduction the generation
Abstracts
distribution and allelic frequencies of polymorphism were determined and compared in primary hyperlipidemic and normolipidemic subjects. Result: The results showed that activity of plasma cholesterol ester transfer was significantly higher in primary hyperlipidemic than in controls (p = 0.001). The genotype and allelic frequencies for this polymorphism differed significantly between primary hyperlipidemic patients and controls (p = 0.022 and p = 0.039, respectively). Conclusion: The genotype and allelic frequencies for D442G polymorphism of cholesterol ester transfer protein gene were statistically different compared to control. Keywords: D442G polymorphism, Cholesterol ester transfer protein activity, Iranian population doi:10.1016/j.clinbiochem.2011.08.946
Poster — [A-10-1229-2] The genotype and allelic frequencies of CETP/MSP1 polymorphism in primary hyperlipidemia patients and control group Hassanzadeh Taghia, Saidijam Massoudb, Latifi Zeinaba, Paoli Maxc a Research Center for Molecular Medicine, Hamadan University of Medical Science, Hamadan, Iran b Molecular Medicine and Genetics Department, School of Medicine, Hamadan University of Medical Science, Hamadan, Iran c School of Pharmacy and Center for Biomolecular Science, University of Nottingham, Nottingham, UK E-mail addresses: [email protected] (H. Taghi), [email protected] (S. Massoud), [email protected] (L. Zeinab), [email protected] (P. Max) Introduction: Primary hyperlipidemia is considered to be major risk factor for pancreatitis, atherosclerosis and coronary heart disease. Gene polymorphism of cholesterol ester transfer protein is known to be associated with changes in lipid levels. Method: Polymerase chain reaction and restriction fragment length polymorphisms were used for genotype detection. Genotype distribution and allelic frequencies of polymorphism were determined and compared in primary hyperlipidemia and normolipidemic subjects. Result: The results showed that activity of plasma cholesterol ester transfer was significantly higher in primary hyperlipidemic than in controls (p = 0.001). The genotype and allelic frequencies for this polymorphism were not statistically different between primary hyperlipidemic patients and controls. Conclusion: The genotype and allelic frequencies for MSP1 polymorphism of cholesterol ester transfer protein gene were not statistically different compared to control. Keywords: MSP1 polymorphism, Cholesterol ester transfer protein activity, Iranian population
doi:10.1016/j.clinbiochem.2011.08.947
Oral — [A-10-1229-3] Effect of cholesteryl ester transfer protein MSP1 polymorphism on serum lipid levels and CETP activity in Iranian primary hyperlipidemic patients Hassanzadeh Taghia, Saidijam Massoudb, Latifi Zeinaba, Paoli Maxc a Research Center for Molecular Medicine, Hamadan University of Medical Science, Hamadan, Iran
b
Molecular Medicine and Genetics Department, School of Medicine, Hamadan University of Medical Science, Hamadan, Iran c School of Pharmacy and Center for Biomolecular Science, University of Nottingham, Nottingham, UK E-mail addresses: [email protected] (H. Taghi), [email protected] (S. Massoud), [email protected] (L. Zeinab), [email protected] (P. Max) Introduction: Cholesteryl ester transfer protein (CETP) gene polymorphism is known to be associated with changes in lipid profiles. Primary hyperlipidaemia is considered to be a major risk factor for pancreatitis, atherosclerosis and coronary heart disease. We studied the effects of MSPI polymorphism of cholesteryl ester transfer on serum lipid levels and CETP activity in Iranian primary hyperlipidemic patients. Methods: This study included 316 Iranian individuals (102 primary hyperlipidaemic patients and 214 healthycontrol). Polymerase chain reaction and restriction fragment length polymorphisms were used for genotype detection. To determine the relationship between MSP1 polymorphism and lipid levels, lipids and CETP activity were measured in primary hyperlipidemic and normolipidaemic subjects, with and without MSP1 polymorphism. Results: Plasma CETP activity was significantly (P< 0.001) higher in primary hyperlipidemic individuals than in controls. Plasma HDL-C was higher in both groups, in the AA genotype than in the GA and GG genotypes, whereas the serum CETP activity was lower in AA genotype compared with other genotypes (GA and GG). In addition Plasma TC was higher in both groups, in the AA genotype than in the GA genotypes. Conclusions: The results showed that MSP1 polymorphism of CETP gene was associated with changes in lipids profile and plasma CETP activity in the selected population and might have a role in contributing to genetic risk of developing coronary artery disease. Keywords: Cholesteryl ester transfer protein, Iranian population, Primary hyperlipidaemia, MSP1 polymorphism doi:10.1016/j.clinbiochem.2011.08.948
Poster — [A-10-1240-1] Investigation about the quantity of reduction the generation time of Cl. novyi (type B) vaccinal strain bacteria (infectious necrotic Hepatitis factor), with increasing various quantity of vitamin solution to culture Lalalizadeh Samaneh Mashhad, Islamic Republic of Iran E-mail address: [email protected] Introduction: Type B Novyi species belongs to clostridia. That is gram-positive and anaerobic which is able to make α fatal toxin. Because of the toxin, it is very important to produce vaccine against the disease. This research tried to reduce the generation time in order to make pure toxin to obtain high quality vaccine. Method: At first we must make culture and then add vitamin solution (biotin, nicotin amid, B1, B12, B6) in order to prepare growing process. Then we incubate foregoing culture for 24 h to control and avoid it from being contaminated. After that vaccinal strain of Cl. novyi (type B) was inoculated in it. At the end, we used the produced toxin to determine MLD after passing 24 h. Results and conclusion: Observations showed that vitamin solution added to culture caused reduction of generation time from 72 to 24 h. In the reduction period (72–24 = 48 h), MLD was promoted, thus, produced toxin had better quality. These observations are very important for making high quality vaccine. In many researches, the role of various vitamins on reduction the generation