The glycoproteins of Acacia erioloba exudates

nted in

TEIN

cacia erioi~ba

ATES~

DAVID W. GAMMON, ALISTAIRM. STEPHEN.AND SHIRLEYC. CHURMS

De~arcmeni of Organic Che~~t~,

~nive~i~

of Cape Town,

ptcd for publication, Au

alactose occ~

~nted, in part, at the Int , and the 8th Int~mational 62151

03.50

rides,

U.S.

in three

15

MON. A. M. STEPHEN. S. C. CHURMS

cia e

~LYCOP

s of concanavalin

D. W. GA

N, A. M. STEP

1 Elu

L)

aphy of A8OP on DEAE-~~1~10~: carbo

STEPHEN,

Elut

1

-

l5

-0.

1

-

5

-

(x5

(mL) &ion with proteinase K: (a) st

eluted ~th M NaCI:

RESULT

S. C. CHURMS

ab~rbance at 220 nm, ----; amino

AND DISCUSSION

ical locations. Since

ucts of 2 su~essive

AC

eriolo 1

G~~COP 2

Fig. 3. SDS-PAGE o (channels 4 and 7), a b, (b) albumin, (c) Channels l-5 were st

a

S 4

s

D. W. GAMMON, A. M. SIPHON, lb)

(a)

A‘ 60%

Amberiite IR- 120 tH+)

Insoluble

SOlUble

Al

saturated

Insoluble

Soluble

A2

eo *I. saturated

(i) dialysed (ii)~reeze-dried

(i 1 dialysed (ii) l~eeze-dried

Scheme

1.

A8305

(i) diolysed (ii)

freeze-dried

ABOP

Scheme 1. Fractionation of gum samp!es A and A’ from A. erioloba.

onents (“a continuous s

S. C. C

Fig. 4. Chromate c~bohydrate, -;

y, on a ~lumn (10 x 0.5 cm) of byd~xyapatite, of (a) A, (b) Al, and (c) A2: ate buffer (pH 7.4), --.-a-. ante at 220 nm, -----; ph

CH20Ac

I

Scheme 2. O~gin of the si

ent ions in the mass 5pectNm of 1.

1

~ETHY~TION

2,3,4,62,3,4,6-

. STEPHEN, S. C. CHU

Al (

ANALYSES FOR MENDED

1) AND PRODU~S

DERIVED ~ERE~OM

5”

1

23h

-

2,3,4,~Gal-

6

2,3,6-Gal 2,3,4-Gal 2,4-Gal Uroni~ acid

14 12 6 21”

39 4 5 -

8 11 11 8 -

30 27 31 12 n.d.

I 8

J1.2”

l~tegrated interim

5. 4. 4.

2.8 3.4 7.3

0.33 1 0.

S

J1.2

integrated interim

5.07

3.4

1

protons not included.

ata insistent

~th th

S

JI,2

integrated interim

5.25

3.4

0.5

4.56 4.54

7.1 7.4

1.5

of Pear

et al.%. cP.p.m. do~fieid

8

J12

l~t~grated i~te~~~

4.52

7.2

1

Assignment

. eJ,,5value is tentative.

1

D. W. GAMMON, A.

STEPHEN, S. C. CHUR

-- ??-I -- 96-l ---------------

(b)

--------

143-==108~ 190-155

J

237--,202J

--=-216---l&31

Scheme

3.

entat rved. mentationz7 in each arises, as expectedz8, from abJ, fragmentation,

not react~~31 in

(b) acid II; m/z val is from te~inal u he de~vative of I includes an ion m/z 314

cac~a erioloba GLYCOPRO~INS

io-Gel P-2) of the pro cts of hydrol~is of PA1 with ba~um hy yproline, ------; optics rotation at 365 nm, -.-.--.-.

17

ON, A. M. STEPHEN.

S. C. C

S

After removal oft

ACKNO

~DGMENTS

RENCES

LLEN, A. NEVBERGER, Fkw~,

Carbo~ydr.

C. L. GILL, C. G. A.

C. L. GILL, A. M.

Poiym., 5 (1985) 251-273. CNAB. AND

G. DE PINTO, Phytochemistry, 23

JEFFREY. AND F. J. MCDOUGALL,

F~ytochemistry, 24

Phytochemist~, 22 (1983) 2481-

171

a GLYCOPRO~INS

BERS, AND

F. Stmw, Anal. Chem.,