- Email: [email protected]
The healing of acute tendon injuries: effects of intrasound therapy and low intensity pulsed ultrasound
524
Abstracts
The healing of acute tendon injuries: effects of intrasound therapy and low intensity pulsed ultrasound A.I. Aiyegbusi
a,
*, F.I.O. Duru a, S.R.A. Akinbo
b
FASCIA SCIENCE AND CLINICAL APPLICATIONS: ABSTRACTS
a Department of Anatomy College of Medicine, University of Lagos, Nigeria1 b Department of Physiotherapy, College of Medicine, University of Lagos, Nigeria2 * Corresponding author. Aiyegbusi A.I. PhD, Dept. of Anatomy, College of Medicine, University of Lagos, Lagos, Nigeria. Tel.: +2348023212513. E-mail address: [email protected] (A.I. Aiyegbusi).
Background and Purpose This study investigated the effects of low and high intensity intrasound therapy (LITR&HITR) given twice daily and Low intensity pulsed ultrasound (LIPUS) on the morphology of the healing tendon.
Methods Forty-five male Sprague-Dawley albino rats, randomized into five groups were further subdivided into groups A and B except for Group 1 which had only one group. Groups 2e5 underwent an induced crush injury. The five groups were allocated to: serve as controls (Group 1), received no treatment (Group 2), LIPUS once daily (Group 3), LITR twice
daily (Group 4), and HITR twice daily (Group 5). Intrasound therapy (ITR) and LIPUS treatment was commenced 24 hours post injury and was given twice daily and once daily respectively over the first 14 days post injury. The animals in subgroup A were sacrificed on day 15 post injury, and those in subgroup B were sacrificed on day 31 post injury. The tendons were excised and processed for histological studies. Estimation of the numerical density of the tendon cells and collagen quantity was performed in sections of tendon tissue embedded in paraffin and stained with H&E.
Results HITR and LITR given twice daily caused a significant (p<0.05) proliferation of tenoblasts in the proliferative phase and enhanced their terminal differentiation to tenocytes in the remodelling phase compared with the untreated and LIPUS treated groups. HITR and LITR also resulted in a significant increase in the volume fraction of collagen fibres compared with LIPUS.
Conclusion Intrasound had a better morphological effect on the healing tendon than LIPUS and may be an option to consider in the treatment of acute tendon injuries.
Key words Intrasound therapy, Low intensity pulsed ultrasound, Tenoblasts, Tendon injury
doi:10.1016/j.jbmt.2012.01.011
Evaluation of the effect of mechanical loading on angiogenic factors in tendinopathy Rouhollah Mousavizadeh *, Vincent Duronio, Bob McCormack, Alexander Scott Department of Medicine and Department of Physical Therapy, University of British Columbia, 772-2635 Laurel Street, Vancouver BC V5Z 1M9, Canada * Tel.: +1 6048755702. E-mail address: [email protected] (R. Mousavizadeh).
Background Tendon disorders are a significant cause of pain and morbidity amongst athletes, workers and the general public (1,2). Tendinopathy is often viewed as the result of failed or inadequate healing response through repetitive overuse (3). The clinical symptoms of tendinopathy are
1 2
Tel.: +2348030537354. Tel.: +2348037140973.
activityerelated pain, focal tenderness, and intratendinous imaging changes. Previous authors have suggested there may be an association between pain and neurovascular changes resulting from tendon overuse in tendinopathy patients (4,5). In order to examine the effects of repetitive overuse on the expression of angiogenic genes which regulate neovascularization in tendinopathy, primary human tendon cells were subjected to cyclic strain.
Methods By using FlexcellÒ Tension Systems, the isolated tendon cells from human hamstring tendons (excess ACL autograft material) were exposed to cyclic tension (1Hz frequency and 10% strain). RNA samples were isolated at different time points and gene expression was evaluated by qPCR. Zymography assay was also conducted in order to measure the activity of MMP-2 in the supernatant of tendon cell culture.
Results Initial experiments show that cyclic strain of two-dimensional primary tenocyte cell cultures (1 Hz) promotes increased expression of VEGF, bFGF, Cox-2 and IL-6 genes,
Abstracts
The healing of acute tendon injuries: effects of intrasound therapy and low intensity pulsed ultrasound A.I. Aiyegbusi
a,
*, F.I.O. Duru a, S.R.A. Akinbo
b
FASCIA SCIENCE AND CLINICAL APPLICATIONS: ABSTRACTS
a Department of Anatomy College of Medicine, University of Lagos, Nigeria1 b Department of Physiotherapy, College of Medicine, University of Lagos, Nigeria2 * Corresponding author. Aiyegbusi A.I. PhD, Dept. of Anatomy, College of Medicine, University of Lagos, Lagos, Nigeria. Tel.: +2348023212513. E-mail address: [email protected] (A.I. Aiyegbusi).
Background and Purpose This study investigated the effects of low and high intensity intrasound therapy (LITR&HITR) given twice daily and Low intensity pulsed ultrasound (LIPUS) on the morphology of the healing tendon.
Methods Forty-five male Sprague-Dawley albino rats, randomized into five groups were further subdivided into groups A and B except for Group 1 which had only one group. Groups 2e5 underwent an induced crush injury. The five groups were allocated to: serve as controls (Group 1), received no treatment (Group 2), LIPUS once daily (Group 3), LITR twice
daily (Group 4), and HITR twice daily (Group 5). Intrasound therapy (ITR) and LIPUS treatment was commenced 24 hours post injury and was given twice daily and once daily respectively over the first 14 days post injury. The animals in subgroup A were sacrificed on day 15 post injury, and those in subgroup B were sacrificed on day 31 post injury. The tendons were excised and processed for histological studies. Estimation of the numerical density of the tendon cells and collagen quantity was performed in sections of tendon tissue embedded in paraffin and stained with H&E.
Results HITR and LITR given twice daily caused a significant (p<0.05) proliferation of tenoblasts in the proliferative phase and enhanced their terminal differentiation to tenocytes in the remodelling phase compared with the untreated and LIPUS treated groups. HITR and LITR also resulted in a significant increase in the volume fraction of collagen fibres compared with LIPUS.
Conclusion Intrasound had a better morphological effect on the healing tendon than LIPUS and may be an option to consider in the treatment of acute tendon injuries.
Key words Intrasound therapy, Low intensity pulsed ultrasound, Tenoblasts, Tendon injury
doi:10.1016/j.jbmt.2012.01.011
Evaluation of the effect of mechanical loading on angiogenic factors in tendinopathy Rouhollah Mousavizadeh *, Vincent Duronio, Bob McCormack, Alexander Scott Department of Medicine and Department of Physical Therapy, University of British Columbia, 772-2635 Laurel Street, Vancouver BC V5Z 1M9, Canada * Tel.: +1 6048755702. E-mail address: [email protected] (R. Mousavizadeh).
Background Tendon disorders are a significant cause of pain and morbidity amongst athletes, workers and the general public (1,2). Tendinopathy is often viewed as the result of failed or inadequate healing response through repetitive overuse (3). The clinical symptoms of tendinopathy are
1 2
Tel.: +2348030537354. Tel.: +2348037140973.
activityerelated pain, focal tenderness, and intratendinous imaging changes. Previous authors have suggested there may be an association between pain and neurovascular changes resulting from tendon overuse in tendinopathy patients (4,5). In order to examine the effects of repetitive overuse on the expression of angiogenic genes which regulate neovascularization in tendinopathy, primary human tendon cells were subjected to cyclic strain.
Methods By using FlexcellÒ Tension Systems, the isolated tendon cells from human hamstring tendons (excess ACL autograft material) were exposed to cyclic tension (1Hz frequency and 10% strain). RNA samples were isolated at different time points and gene expression was evaluated by qPCR. Zymography assay was also conducted in order to measure the activity of MMP-2 in the supernatant of tendon cell culture.
Results Initial experiments show that cyclic strain of two-dimensional primary tenocyte cell cultures (1 Hz) promotes increased expression of VEGF, bFGF, Cox-2 and IL-6 genes,